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Spirakel
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 20861346     EISSN : 23548819     DOI : https://doi.org/10.22435/spirakel
Core Subject : Health,
Public Health
SPIRAKEL is a media for researchers / academics / students / practitioners of Health Office, Department of Health, Public Health Service Center, to obtain or disseminate scientific information on tropical infectious diseases.
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5 Documents
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Search results for , issue "Vol 10 No 1 (2018)" : 5 Documents clear
DETEKSI TRANSMISI TRANSOVARIAL VIRUS DEN-3 PADA NYAMUK Aedes aegypti DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10 Tri Wahono; Sitti Rahmah Umniyati
SPIRAKEL Vol 10 No 1 (2018)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

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Abstract

Transovarial transmission is a phenomenon as a supporting factor for the maintenance the presence of dengue virus in an area. Vector surveillance is an effort to control dengue disease. In addition to larvae surveillance, viral surveillance carried out on adult Ae. aegypti mosquitoes can actually be used for early warning systems for dengue outbreaks. DEN 3 virus antigen detection using streptavidin biotin peroxidase complex (SPBC) immunocytochemical technique is an alternative method for vector surveillance. The study aimed to prove the existence of transovarial transmission by detecting DEN 3 virus antigen in F1 generation mosquitoes from Ae. aegypti which has been infected orally. The study design was experimental. Adult Ae. aegypti female is infected with DEN 3 virus orally then mosquitoes are allowed to run their gonotrophic cycle. The resulting egg was colonized until becoming adult mosquitoes and DEN 3 virus antigen was detected. Antigen detection using SPBC immunocytochemical technique with DSSE10 monoclonal antibody on mosquito head squash preparation in 4 and 16 days old mosquitoes. The results were analyzed descriptively. Streptavidin biotin peroxidase complex (SBPC) immunocytochemical technique can detect DEN 3 virus antigens indicated by the presence of brown color in the head squash preparation. The presence of the DEN 3 virus antigen also proves the presence of transovarial transmission in infected Ae. aegypti mosquitoes which has been infected orally.
BERBAGAI ASPEK TENTANG MALARIA DI KABUPATEN PESAWARAN, PROVINSI LAMPUNG Ritawati Ritawati; Yanelza Supranelfy
SPIRAKEL Vol 10 No 1 (2018)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

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Abstract

Malaria is one of infectious diseases that still affect health problems in Indonesia. Pesawaran District, Lampung is a malaria endemic area, Annual Parasite Insidense (API) for 5 years (2013-2017) including areas with High Case Insidence (HCI) because it has API> 5 ‰. Efforts are made towards malaria-free areas that is integrated malaria control. This requires local entomological data primarily related to species, malaria morbidity, community behavior towards malaria and data on the use of anti-malarial drugs. The method used in the writing of literature study through the study of books, articles, and scientific journals from 2008 to 2017. API Pesawaran District in 2017 highest is 7,5‰ for 10 years (2008-2017 ). Anopheles spp, found in Pesawaran District are Anopheles barbumbrosus, An. nigerrimus, An.nitidus, An peditaeniatus, An.sundaicus, An. barbirostris, An. annularis, An. minimus, An. kochi, An. aconitus, An. tessellatus, An. vagus, An. subpictus, An.indefinitus, An. maculatus and An. hyrcanus group. Community attitudes found that the variables associated with malaria incidence are the habit of going out at night, the installation of wire netting, the density of the walls of the house, the use of mosquito nets at night, the use of anti-mosquito / repellent and the use of body covering. The use of ACT as an anti malarial drug especially Falciparum in Pesawaran District, Lampung is still adequately with highest failure prevalence was 11.59% and the lowest at 2.1%.
EFEKTIVITAS GRANUL DAUN SALAM (Eugenia polyantha Wight) SEBAGAI LARVASIDA NYAMUK Aedes aegypti Priadi Eling Waskito; Widya Hary Cahyati
SPIRAKEL Vol 10 No 1 (2018)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

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Abstract

The usage of synthetic larvicide has many weakness, such as causing resistance. The alternative way to reduce the unwanted impact by using biolarvicide from plants such as the bay leaf (Eugenia polyantha Wight). The purpose of this research is to find out the larvicidal effect of bay leaf extract granule againts Aedes aegypti. The study was conducted with true experiment method with post test only control group design. The population of this research was Ae. aegypti larvae instar III in IVRCRD Salatiga. Total sample were 600 larvae used. The data was analyzed with univariate and bivariate analysis (Kruskal Wallis) by using SPSS. The result showed that there was correlation between bay leaf extract granule due to larvae mortality (p=0.001). The value of LC50 of bay leaf extract granule is 3,646.770 mg and LC90 is 9,430.171 mg. The value of LT50 of 3,500 mg is 27.462 hours, while LT90 is 4.778E3 hours. Based on acute toxicity test, it has affected to Ae. aegypti larvae.
EFEKTIVITAS KELAMBU BERINSEKTISIDA TERHADAP NYAMUK An.sundaicus (DIPTERA: CULICIDAE) DAN PENGGUNAANNYA DI DESA SUNGAI NYAMUK, KALIMANTAN UTARA Sugiarto Sugiarto; Upik Kesumawati Hadi; Susi Soviana; Lukman Hakim
SPIRAKEL Vol 10 No 1 (2018)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

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Abstract

Long lasting insecticide nets (LLiNs) are used in vector control programs to malaria elimination. Maintenance of LLiNs is an important factor to ensure the effectiveness of the bed nets. This study aimed to analyze the effectiveness of LLiNs against An. sundaicus and analyze knowledge, attitudes, practises (KAP). The research was conducted in the Sungai Nyamuk Village, Sebatik Sub District, Nunukan District - North Kalimantan. The method used in this research is the WHO bioassay cone test and questionnaires of KAP for the use of LLiNs. The efficacy test consists of the treatment and control groups. Treatment group was LLiNs that have been used for 6 months, 12-23 months and over 24 months, while the control group was bed nets without insecticide. The results showed that LLiNs have been used for 6 months had the highest effectiveness with mortality 24 hours and 1 hour knockdown An. sundaicus amounted to 94,13%. While LLiNs have been used for 12-23 months showed ineffective with 24-hour mortality is 71,74%, LLiNs more than 24 months (mortality 24 hours is 37,33%). KAP results showed a 100% respondents to accept the distribution of LLiNs, but not willing to wash the nets. The effectiveness of LLiNs is correlated with washing nets. The use of LLiNs to prevent malaria transmission will be effective if supported by good maintenance.
STUDI KUALITATIF PERAN PETUGAS KESEHATAN DAN KADER DESA DALAM PROGRAM PENGOBATAN MASSAL FILARIASIS DI KABUPATEN MAMUJU UTARA PROVINSI SULAWESI BARAT TAHUN 2015 Made Agus Nurjana; Hayani Anastasia; Rosmini Rosmini; Sitti Chadijah; Mujiyanto Mujiyanto; Ni Nyoman Veridiana; Octaviani Octaviani; Leonardo Taruk Lobo
SPIRAKEL Vol 10 No 1 (2018)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

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Abstract

The role of health workers and health volunteer are essential in the implementation of mass drug administration (MDA) in North Mamuju District. Several activities were conducted prior MDA, such as advocacy to policy makers (e.g regent) and socialization from district level to village level. Qualitative research methods and datacollection were carried out for six months. Interviewed to 17 health workers and 60 health volunteers were conducted by using an open questionnaire, which aimed to know the role of each health workers and health volunteers in the implementation of MDA for the last two years. The study result showed that the role of health workers and health volunteers was not fully optimised because the lack of budget and human resource. Therefore, to optimise the role of health workers and health volunteers, the authority needs to consider to allocate a sufficient budget and human resource for the implementation of MDA

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