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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 19, No 1 (2014): February 2014" : 15 Documents clear
Viabilitas Bakteri Asam Laktat dan Aktivitas Antibakteri Produk Susu Fermentasi Komersial terhadap Beberapa Bakteri Patogen Enterik Ekawati Purwijantiningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.450

Abstract

AbstractFermented milk products could be found on the marketplace in great quantities of brand and type. Fermented milk has potentials for functional food because it has health benefits for human body and acts as antimicrobial. However from all brands and types, the viability of lactic acid bacteria (LAB) and antibacterial activity is not surely known, especially in Yogyakarta. The aim of this study was to determine the viability of lactic acid bacteria and antibacterial effect to three enteric pathogenic bacteria. Tests were carried out on 18 samples of fermented milk from supermarkets in Yogyakarta. Base on LAB viability test, 12 samples fulfilled the concentration of probiotic bacteria, number of viable bacteria at least 108 CFU/ml. Antibacterial activity from 18 brands against Staphylococcus aureus, Escherichia coli and Salmonella sp. by using well diffusion method. Fermented milk products have different antibacterial activities. Brand R showed the maximum antibacterial effect against the pathogens. It was determined that the most sensitive pathogenic bacteria to milk fermented products were S. aureus, whereas the least sensitive pathogen was Salmonella sp.Keywords: Antibacterial, fermented milk, viability of lactic acid bacteriaAbstrakProduk susu fermentasi dapat ditemukan di pasaran dalam berbagai merk dan jenis. Susu fermentasi tersebut berpotensi sebagai pangan fungsional karena memiliki manfaat kesehatan bagi manusia dan berperan sebagai antimikrobia. Akan tetapi dari berbagai merk dan jenis yang ada belum diketahui secara pasti mengenai viabilitas bakteri asam laktat (BAL) dan aktivitas antibakterinya terutama yang beredar di wilayah Yogyakarta. Penelitian ini bertujuan mengetahui viabilitas BAL dan efek antibakteri terhadap tiga bakteri patogen enterik. Uji dilakukan pada 18 sampel susu fermentasi yang berasal dari supermaket di kota Yogyakarta. Berdasarkan uji viabilitas BAL, ada 12 sampel yang memenuhi standar konsentrasi bakteri probiotik,paling tidak mengandung 108 CFU/ml. Aktivitas antibakteri dari 18 merk diuji terhadap Staphylococcus aureus, Escherichia coli dan Salmonella sp. menggunakan metode difusi sumuran. Produk minuman susu fermentasi memiliki kemampuan antibakteri yang berbeda. Merk R menunjukkan efek antibakteri paling tinggi terhadap beberapa bakteri patogen. Bakteri patogen yang paling sensitif terhadap produk susu fermentasi adalah S. aureus, yang paling tidak sensitif adalah Salmonella sp.Kata kunci: Antibakteri, susu fermentasi, viabilitas BAL
Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Teguh Budipitojo; Hevi Wihadmadyatami; Ariana -; Dewi K. Musana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.451

Abstract

AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia
Hubungan Kepadatan dan Biting Behaviour Nyamuk Anopheles farauti Dengan Kasus Malaria di Ekosistem Pantai dan Rawa (Kabupaten Biak Numfor dan Asmat) Hanna S.I. Kawulur; Hidayat Soesilohadi; Suwarno Hadisusanto; Y. Andi Trisyono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.452

Abstract

AbstractPopulation density and bitting behaviour of insect vectors are several of the factors that influence the number of cases of malaria. This study aims to determine the relationship between population density and bitting behaviour Anopheles farauti which is a vector of malaria in coastal ecosystems (Biak Numfor Regency) and swamp ecosystems (Asmat Regency) with malaria cases. The method used is human landing collection conducted at 18:00 to 6:00 a.m. inside and outside the house. The results showed that the population density of An. farauti in coastal ecosystems is relatively lower than the swamp ecosystems. Man bitting rate in coastal ecosystems is 4 and 4.66, at 95.52 and 42.38 in swamp ecosystem. An. farauti on two ecosystems research are eksofilik. Population density and bitting behaviour An. farauti in coastal ecosystems and swamp ecosystems are not positively correlated with the number of malaria cases.Keywords: population density, biting behavior, An. farauti, Biak Numfor, AsmatAbstrakKepadatan populasi dan aktivitas menggigit serangga vektor merupakan salah satu faktor yang mempengaruhi jumlah kasus malaria. Penelitian ini bertujuan menentukan hubungan kepadatan populasi dan aktivitas menggigit Anopheles farauti yang merupakan vektor malaria di ekosistem pantai (Kabupaten Biak Numfor) dan ekosistem rawa (Kabupaten Asmat) dengan kasus malaria. Metode yang digunakan adalah human landing collection yang dilakukan pada pukul 18.0006.00 di dalam dan di luar rumah. Hasil penelitian menunjukkan bahwa padat populasi An. farauti di ekosistem pantai relatif lebih rendah dibandingkan ekosistem rawa. Man bitting rate di ekosistem pantai adalah 4 dan 4,66 di ekosistem rawa 95,52 dan 42,38. An. farauti pada dua ekosistem penelitian bersifat eksofilik. Kepadatan populasi dan aktivitas mencari darah An. farauti di ekosistem pantai dan ekosistem rawa tidak berkorelasi positif dengan jumlah kasus malaria.Kata kunci: kepadatan populasi, aktivitas menggigit, An. farauti, Biak Numfor, Asmat
Komposisi Jenis dan Kepadatan Sponge (Porifera: Demospongiae) di Kepulauan Spermonde Kota Makassar Abdul Haris; Shinta Werorilangi; Sulaiman Gosalam; Andry Mas’ud
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.453

Abstract

AbstractSponge is one of the components on the coastal and marine ecosystems, especially coral reefs and seagrass beds. Climate change and environmental conditions can affect the life of the sponge. The study is conducted to determine the distribution of the composition and density of sponge using belt transects (transect quadrant) with a size of 5x5 m, then count the number of each type of sponge contained in the transect. Study site divided into three (3) zones indicating different conditions of eutrophication. A total of 49 species were identified from 16 families of 8 orders. Composition and density of the sponge in inner zone as many as 11 families with a density of 0.96 ind/m2, and lower compared to the composition and density in the middle zone and outer zone. This is related to the difference in environmental conditions of the three zones based on eutrophication conditions.Keywords: Sponge, species composition, density, spermondeAbstrakSponge merupakan salah satu penyusun pada ekosistem pesisir dan laut, terutama pada ekosistem terumbu karang dan padang lamun. Perubahan iklim dan kondisi lingkungan dapat mempengaruhi kehidupan dari sponge. Maka dilakukan penelitian untuk mengetahui sebaran komposisi dan kepadatan sponge menggunakan metode transek belt (transek kuadran) dengan ukuran 5x5 m, kemudian menghitung jumlah dari setiap jenis sponge yang terdapat dalam transek. Lokasi penelitian terbagi atas 3 (tiga) zona eutrofikasi yang menunjukkan kondisi eutrofikasi yang berbeda. Sebanyak 49 spesies yang teridentifikasi berasal dari 16 famili 8 ordo. Komposisi dan Kepadatan sponge pada zona dalam sebanyak 11 famili dengan kepadatan 0,96 ind/m2,, lebih rendah dibandingkan dengan komposisi dan kepadatan pada zona tengah dan zona luar. Hal tersebut terkait dengan adanya perbedaan kondisi lingkungan dari ketiga zona yang terbagi berdasarkan kondisi eutrofikasi tersebut.Kata kunci: Sponge, komposisi jenis, kepadatan, spermonde
Kondisi Terumbu Karang di Kawasan Taman Nasional Laut Kepulauan Seribu DKI Jakarta Drajad Sarwo Seto; Djumanto -; Namastra Probosunu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.454

Abstract

AbstractThe objective of this research was to determine the condition of coral reefs, namely the percentage of coral covered, species distribution, community structure of coral, and the environmental conditions surround the core zone, protection zone, tourism zone, and residential zone in Kepulauan Seribu Marine National Park DKI Jakarta. The research was conducted from 8 to 16 May, 2013. The data was collected from four zones and each zone was set up into two stations as a point observation, at the depth of 79 meters. The percentage of coral coverring was calculated by line intercept transect method, coral genera was counted and identified using the belt transect method. Coral data was analyzed qualitatively based on ecological index. The results showed that the coral covering was range from 7.25 to 68.93% as categorized from bad to good condition. The number of coral was found approximately of 5.523 colonies that consisted of 45 genera and 16 families. The most abundance of coral was Porites and Montipora with percentage of 19.7% and 16.69%, respectively. Coral diversity index was ranged from 1.61 to 3.07 as indicated of low to high. Uniformity index was ranged from 0.44 to 0.68, which was the community in stressful to labile situation. Dominance index (D) was ranged from 0.06 to 0.32 showing that coral dominance was absence.Keywords: Coral reef, cover, diversity, Kepulauan SeribuAbstrakPenelitian ini bertujuan mengetahui kondisi terumbu karang yang meliputi persentase tutupan, sebaran, struktur komunitas dan kondisi lingkungan di zona inti, perlindungan, pemanfaatan wisata, dan pemukiman di kawasan Taman Nasional Laut Kepulauan Seribu DKI Jakarta. Penelitian dilaksanakan dari tanggal 8 sampai 16 Mei 2013. Pengambilan data berada pada empat zona pengelolaan dan setiap zona ditetapkan sebanyak dua stasiun pengamatan pada kedalaman 79 meter. Persentase tutupan karang dihitung dengan metode Line Intercept Transect, genera karang dihitung dan diidentifikasi menggunakan metode Belt Transect. Data jenis karang yang diperoleh dianalisis kualitatif berdasarkan indeks ekologis. Hasil penelitian diperoleh persentase tutupan karang berada pada kisaran 7,2568,93% yang dikategorikan kondisinya buruk hingga baik. Jumlah karang dari seluruh stasiun penelitian sebanyak 5.523 koloni yang terdiri dari 45 genera dan 16 famili. Genus karang yang paling sering dijumpai adalah Porites dan Montipora dengan persentase kelimpahannya masing-masing 19,7% dan 16,69%. Nilai indeks keanekaragaman (H’) karang berkisar antara 1,613,07 yang tergolong rendah hingga tinggi. Indeks keseragaman berkisar 0,440,68 yang berarti komunitas dalam keadaan tertekan hingga labil. Nilai indeks dominansi (D) berkisar 0,060,32 yang menunjukkan dominansi karang tertentu tergolong rendah.Kata kunci: Tutupan, terumbu karang, keragaman, Kepulauan Seribu

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