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INDONESIA
Jurnal Biotek Medisiana Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 23015810     EISSN : 23548800     DOI : -
Core Subject : Health,
Health Professions
Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of non-communicable diseases and infectious.
Arjuna Subject : -
Articles 6 Documents
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Search results for , issue " Vol 1, No 2 (2012)" : 6 Documents clear
Kemampuan Direct Current Yang Diubah Menjadi Alternating Current Dalam Menghambat Perkembangan Telur Nyamuk Aedes Aegypti Hakim, Lukman; Pujiastuti, Endang; Kusnandar, Asep Jajang
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

AbstractPre-adult dengue vector is controlled through management of the environment and the use of insecticides, although they have not yet entirely reduced dengue cases. Development of method to control dengue vector physically have been conducted using electric current device converting DC into AC current.The study was conducted in three stages, aimed to find the effectiveness of the device applied with 8 electric current (AC) strengths, the application of time-contact of the current (8 kinds), and water volume (8 kinds) in the container of the device, in killing Aedes aegypti eggs.The results showed, the device could inhibit the development of Aedes aegypti eggs 86.7% at average. The most effective electric strength to kill 50% mosquitoes’ egg was 1.2 volt. On the other hand, variation of time-contact application and water volume of the device did not influence the effectiveness of the device.Since the device could not inhibit the development of Aedes aegypti eggs up to 95%, it was concluded that the AC device is not effective against aquatic stages (pre-adult) mosquito, and cannot be developed as a device to control dengue vector.Keywords: electricity, mosquito eggs, dengue fever, vector eradicationAbstrakPemberantasan vektor Dengue stadium pra dewasa, dilakukan melalui penataan lingkungan dan penggunaan insektisida, meskipun belum sepenuhnya dapat menurunkan kasus DBD. Saat ini telah dilakukan pengembangan secara fisik metode pengendalian vektor DBD menggunakan perangkat listrik dengan mengubah arus listrik DC menjadi AC. Penelitian ini dilakukan dalam tiga tahap, yang bertujuan untuk menemukan efektivitas perangkat listrik dalam membunuh telur Aedes aegypti dengan menggunakan arus listrik 8 variasi (AC) kekuatan, yang diaplikasikan saat ini dengan waktu-kontak (8 variasi), dan volume air container (8 variasi). Hasil penelitian menunjukkan, perangkat listrik tersebut dapat menghambat perkembangan telur Aedes aegypti rata-rata 86,7%. Kekuatan listrik yang paling efektif untuk membunuh telur nyamuk sampai 50% adalah 1,2 volt. Di sisi lain, variasi dari alat listrik ini yang diaplikasikan dengan waktu-kontak dan volume air tidak berpengaruh terhadap efektivitas perangkat listrik. Karena perangkat listrik tersebut tidak dapat menghambat perkembangan telur nyamuk Aedes aegypti sampai 95%, maka dapat disimpulkan bahwa perangkat arus listrik AC tidak efektif terhadap pemberantasan nyamuk pada stadium (pre-dewasa), dan tidak dapat dikembangkan sebagai perangkat listrik untuk mengendalikan vektor DBD.Kata kunci: Arus listrik, Telur nyamuk, Demam berdarah, Pemberantasan vektor
Perbedaan Warna Kontainer Berkaitan dengan Keberadaan Jentik Aedes aegypti di Sekolah Dasar Budiyanto, Anif
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

Containers inside and outside the school building are breeding places for Dengue vector. Presence or absence of Ae aegypti mosquito larvae in a container could be affected by the type, location, color, material, condition and volume of container lid. The purpose of this study was to determine the condition of container that affect the presence of Ae aegypti mosquito larvae. A cross sectional survey was conducted to collect mosquito larvae in primary schools in the district of Ogan Komering Ulu, South Sumatera Province with a single larvae method. Mosquito larvae were found in 54% of surveyed-schools (54%), and mostly were Aedes aegypti larvae (91%). There was a significant correlation between dark and light color differences of the water container in the presence of mosquito larvae (P value = 0.017, CI 95% = 1.2 to 2.9). On the other hand, there was no significant correlation between different types of containers, location of containers, material of containers, the container lid and the volume of water in a container in the presence of larvae. There was a significant correlation between the difference in container’s color (light and dark) in the presence of Ae aegypti mosquito larvae. It is recommended to have regular cleaning of the containers in order to prevent dirt or mosses covered the containers which will impress of being dark color.Key words: dengue, Aedes aegypti, larvae, containers, school. AbstrakKontainer bagian dalam dan luar sekolah menjadi tempat perindukan vektor Dengue. Ada tidaknya larva Aedes aegypti didalam kontainer dapat dipengaruhi oleh tipe, lokasi, warna, material kondisi dan volume dari kontainer. Tujuan dari penelitian ini untuk menentukan kondisi kontainer yang dapat mempengaruhi adanya larva Aedes aegypti. Penelitian ini adalah survey Cross seksional yang dilakukan dengan mengumpulkan larva nyamuk di sekolah dasar di Kabupaten Ogan Komering Ulu di Sumatera Selatan dengan metode larva tunggal. Larva nyamuk ditemukan pada 54% sekolah yang di survey, dan terutama adalah larva Aedes aegypti (91%). Tedapat korelasi signifikan adanya larva nyamuk diantara kontainer yang berwarna gelap dan terang (P value = 0,017, CI 95% = 1,2-2,9). pada sisi lain ditemukan korelasi yang tidak signifikan antara tipe, lokasi, material, ukuran dan volume air dalam kontainer terhadap keberadaan larva .Terdapat korelasi yang signifikan antara kontainer yang berwarna gelap dan terang dan keberadaan larva nyamuk Aedes aegypti. Direkomendasikan untuk selalu membersihkan kontainer supaya kontainer tidak memberikan warna gelap.Kata kunci: dengue Aedes aegyti, larva, container, sekolah
Sebaran Serotipe Virus Dengue di Pontianak, Medan dan Jakarta Tahun 2008 Herman, Reni; Utami, Basundari Sri; Tuti, Sekar; Novriani, Harly
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

The severity of clinical manifestation of dengue infection depends on the 4 virus serotypes. The aim of the study is to explore the distribution of dengue virus serotypes in 3 cities (Pontianak, Medan and Jakarta) in 2008.The study was a cross-sectional designed; data were obtained from each 2 hospitals in Pontianak, Medan and Jakarta. Sera samples were collected from patients visiting internal and pediatric units in the hospital, which met the inclusion criteria, i.e. had fever for 2 – 7 days, with hemorrhagic manifestation, and/or thrombocyte level less than 100.00/mm3, haematocrit >20% , and had informed-consent signed. Sample sizes were 90 patients from each hospital. About 5 mL blood samples were collected, and serum were separated for RT-PCR testing to determine virus serotype. About 244 sera were collected, i.e. 95 sera from Pontianak, 86 sera from Medan, and 65 sera from Jakarta. Patients visiting hospital mostly had fever for 4 days. More than 60% of RT-PCR tested sera were dengue positive; with the serotype composition Den-3, Den-2 and 3, Den-3 and 1, respectively in Pontianak, Medan and Jakarta.Four dengue virus serotype circulated in Pontianak, Medan and Jakarta, with the majority of serotype 3 (Den-3) in Pontianak and Jakarta, and serotype 2 (Den-2) in Medan.Key words: DHF, dengue, serotype AbstrakDi Indonesia penyakit Demam Berdarah Dengue (DBD) sampai saat ini masih merupakan salah satu masalah kesehatan masyarakat, terutama di kota-kota besar. Tujuan dari penelitian ini adalah untuk mengetahui sebaran serotipe virus dengue tahun 2008 di kota Pontianak, Medan dan Jakarta. Desain penelitian potong lintang. Data yang dikumpulkan masing-masing dari 2 Rumah Sakit di Medan, Pontianak dan Jakarta. Sampel sera dikumpulkan dari penderita yang datang kebagian Penyakit Dalam dan bagian Anak, kriteria demam 2 sampai 7 hari, dengan manifestasi perdarahan dan atau trombosit < 100.000/mm3, Hematokrit> 20% nilai normal serta menandatangani informed concent. Jumlah sampel dari masing-masing Rumah Sakit 90 sampel. Darah penderita diambil maksimal 5 ml, serum dipisahkan dan dilakukan pemeriksaan RT-PCR untuk menentukan serotype virus. Sampel yang terkumpul pada penelitian ini 244, 95 sera dari Pontianak, 86 sera dari Medan dan 65 sera dari Jakarta. Penderita datang ke Rumah Sakit terbanyak setelah 4 hari demam. Dari Hasil pemeriksaan RT-PCR, lebih dari 60% positif, dengan komposisi serotipe terbanyak di Pontianak dengue 3, di Medan dengue 2, diikuti dengue 3 dan di Jakarta dengue 3 diikuti dengue 1.Dari hasil penelitian ini disimpulkan bahwa keempat serotipe virus dengue bersirkulasi di Pontianak, Medan dan Jakarta, dengan mayoritas virus dengue serotipe 3 di Pontianak dan Jakarta, serotipe 2 di Medan.Kata kunci:DBD, dengue, serotype
Infeksi Virus Dengue Tanpa Gejala pada Keluarga Penderita DBD di Provinsi Jawa Barat Santya, Roy NRE; Riandi, M. Umar
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

Dengue Hemorrhagic Fever (DHF) in Indonesia shows an increasing trend since it was first reported in 1968. Based on data from 2003 to 2007, all districts in West Java province had reported outbreak of DHF. Dengue virus transmission is kept up by the presence of virus in the infective host in human and/or mosquitoes. A cross-sectional research was conducted to obtain the incidence of asymptomatic dengue infections in family members of the individual infected. Data was extracted from respondents who were family members of patients that showed immunological response to dengue virus infection. Out of 979 family members of patients tested, 183 (18.7%) individual showed asymptomatic positive immunological response of dengue virus infection. Respondents who leave in a district have risk to be infected 1.35 fold greater than those who leave in a city (p= 0,043; 95% CI =1,004-1,805). There is no significant risk to be infected among gender (female and male respondent) and age groups (≤ 15 years and > 15 years).There is a latent potential problem of sustainable dengue virus transmission in West Java Province. This condition requires attention because the existence of positive individuals without symptoms and their mobility play a role as a reservoir of dengue virus.Key words: dengue virus infection, asymptomatic, immunological responses AbstrakDemam Berdarah Dengue di Indonesia menunjukkan tren meningkat sejak dilaporkan sejak tahun 1968. Berdasarkan data tahun 2003-2007 seluruh Kabupaten di Jawa Barat melaporkan adanya KLB DBD. Transmisi virus dengue terus ada karena ada penularan virus antar manusia melalui nyamuk. Penelitian ini adalah Cross seksional yang dilakukan untuk mengetahui adanya insidens dengue tanpa gejala diantara anggota keluarga dari orang yang terinfeksi. Data diperoleh dari responden yang merupakan keluarga dari responden yang menunjukkan adanya respon imun terhadap virus dengue. Dari 979 anggota keluarga yang di test sebanyak 183 (18,7%) menunjukkan adanya respon imun terhadap virus dengue. Responden yang tinggal di kabupaten yang mempunyai risiko terinfeksi sebesar 1,35 lebih tinggi dari pada yang tinggal di dalam kota (p= 0,043; 95% CI = 1,004-1,805). Tidak ada risiko terinfeksi antara gender, kelompok umur (<15 tahun dan >15 tahun) yang signifikan. Terdapat problem laten untuk transmisi virus dengue yang terus menerus di Jawa Barat. Kondisi ini membutuhkan perhatian karena keberadaan individu tanpa gejala dan mobilitasnya berperan sebagai resevoa virus dengue.Kata kunci: Infeksi virus dengie, asymptomatic, respon imun
Virus-Virus Saluran Pernapasan yang Paling Banyak Ditemukan pada Anak Balita Pasien SARI (Severe Acute Respiratory Infections) -, Widoretno; Saragih, Siti Mariani; Lokida, Dewi
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

In children, 50% respiratory infection was caused by viruses. This research was aimed to identify type of viruses causing respiratory infection among SARI patients under five years old in several regions in Indonesia.Study design of this reserach was prospective study using survey and laboratory analysis. Data was being collected from SARI surveillance. There were 215 throat specimens from 350 children under five years old patients. Specimens were collected from March 2008 to December 2008. To detect viruses in the respiratory tract, this research used Multiplex Bead Array Assay technique.Among 215 specimens tested, 91 specimens (40.12%) were positive respiratory viruses. In children under 1 year old, Coxsakie and Enterovirus (8.72%) were two viruses that mostly detected. In children aged 2 to 3 years old, the virus that mostly found was Respiratory Syncytial virus. And in children aged 4 to 5 years old, Entero and Rhinovirus were mostly found (14.29%). Based on the distribution of respiratory viruses in hospital, there were two peaks shown in the graphs. Firstly, Entero and Rhinovirus were high in Kupang and DKI Jakarta. Secondly, Respiratory Syncytial virus was found high in Kupang, Semarang, and DKI Jakarta.Respiratory Syncytial, Coxsakie, Enterovirus, and Entero Rhinovirus were mostly identified in SARI Patients under five years old.Key word: respiratory viruses, SARI, children under five years AbstrakPada anak-anak lima puluh persen penyebab infeksi saluran pernapasan adalah virus. Tujuan dari penelitian ini adalah unutk mengetahui jenis virus-virus pernapasan pada pasien balita rawat inap penderita SARI (Severe Acute Respiratory Infectiouns) di beberapa daerah di Indonesia. Desain penelitian prospektif dengan jenis studi survey dan studi laboratorium. Data berasal dari spesimen tersimpan surveilans SARI. Jumlah spesimen 215 usap tenggorok dari total 350 pasien balita. Spesimen dikumpulkan dari bulan Maret 2008-Desember 2008. Untuk mendeteksi virus-virus pada saluran pernapasan digunakan teknologi Multiplex Bead Array Assay. Diantara 215 spesimen, 91 spesimen (40,12 %) positif virus pernapasan. Pada anak dibawah 1 tahun, virus yang paling banyak ditemui adalah virus Coxsakie dan Entero Virus (8,72 %). Sedangkan pada anak usia 2-3 tahun virus yang paling banyak ditemukan adalah virus Respiratory Syncytial (17,24 %) dan pada anak usia 4-5 tahun virus yang paling banyak ditemukan adalah virus Entero dan Rhinovirus (14,29 %). Berdasarkan distribusi virus pernapasan di rumah sakit terlihat 2 puncak grafik yang menunjukkan tingginya virus Entero dan Rhino di Daerah Kupang dan DKI. Puncak grafik kedua yang menunjukkan tingginya virus Respiratory Syncytial di Daerah Kupang, Semarang dan DKI. Virus Respiratory Syncytial, Coxsakie dan entero virus serta Entero-Rhinovirus paling banyak ditemukan pada pasien balita SARI.Kata kunci: virus pernapasan, SARI, anak balita
Kadar Kolesterol Tinggi Dan Faktor-Faktor Yang Berpengaruh Terhadap Kadar Kolesterol Darah Soleha, Maratu
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

The cholesterol level in blood has become one of the risk factors of heart disease. One of the risk factors of heart disease is high cholesterol level. This article analysis variables that influence high cholesterol level in blood. Data were obtained from national health research in 2007 . Data consist of 21.178 respondents was clean up become 4.566 clean data ready to be analyze. Study design was cross sectional then analyzed with stata v. 9 followed by backward stepwise selection.Bivariate analysis shows gender, body mass index, sistole and diastole blood pressure, smoke habits and the responden who lack of strenuous activities have a tendency to hypercholesteromia risk.Key words: Hiperkolesterolemia, Indeks Massa Tubuh (IMT), sistolik, diastolik. AbstrakKadar kolesterol dalam darah yang tinggi merupakan salah satu faktor risiko penyakit jantung. Artikel ini membahas berbagai variabel yang mempengaruhi tingginya kadar kolesterol di dalam darah. Data untuk analisis diperoleh dari riset kesehatan dasar tahun 2007. Data terdiri dari 21.178 responden yang dibersihkan sehingga menjadi 4.566 data yang siap untuk dianalisis. Studi desain crossectional. Data dianalis dengan stata v.9 dilanjutkan dengan backward stepwise selection. Analisis dua variabel menunjukkan jenis kelamin, Indeks massa tubuh, tekanan darah, kebiasaan merokok dan kurangnya latihan fisik memiliki risiko hypercholesteromia.Key words: Hiperkolesterolemia, sistolik, diastolik.

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