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All Journal Media Penelitian dan Pengembangan Kesehatan Jurnal Biotek Medisiana Indonesia Jurnal Kefarmasian Indonesia
Reni Herman
Basic Technology Center for Biomedical and Health , the Agency for Health Research and Development
Chemistry Decision Sciences, Operations Research & Management Health Professions Medicine & Pharmacology Public Health

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DETEKSI P.VIVAX SINGLE NUCLEOTIDE POLYMORPHISM (SNP) Y976F DARI SAMPEL MONITORING PENGOBATAN DIHIDROARTEMISININ-PIPERAKUIN DI KALIMANTAN DAN SULAWESI Salwati, Ervi; Herman, Reni; Handayani, Sarwo; Tjitra, Emiliana
Media Penelitian dan Pengembangan Kesehatan Vol 22, No 3 Sep (2012)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/2905

Abstract

Abstract This study was a part of the activity of monitoring Dihydroartemisinin-Piperaquine (DHP) treatment in subjects infected with P.falciparum and P.vivax in Kalimantan and Sulawesi. SNP Y976F had been proved as the mutation in pvmdr1 gene which was related to P. vivax resistance chloroquine in Papua. Data of spreading pvmdr1 SNP Y976F outside Papua is needed for using Dihidroartemisinin-Piperakuin policy in the treatment of vivax malaria in Indonesia. Detection of SNP Y976F was done against 95 day0-samples of subjects confirmed infected with P.vivax or mixed infection of P.vivax and P.falciparum by PCR. The results showed that 88 (93%) of a total 95 samples were positive detected 976F mutant which were distributed in all sentinel sites of West Kalimantan (2of 3), Central Kalimantan (6 of 8), North Sulawesi (63 of 65), and Central Sulawesi (17 of 19).  In conclusion,  pvmdr1 SNP Y976F has been spreaded in all sentinel sites. Key words: P.vivax, pvmdr1, Single Nucleotide Polymorphism Abstrak Penelitian ini merupakan bagian kegiatan dari monitoring pengobatan Dihidroartemisinin-Piperakuin (DHP) pada subyek yang terinfeksi dengan P.vivax atau infeksi campuran P.falciparum dan P. vivax di Kalimantan dan Sulawesi. SNP Y976F merupakan mutasi pada gen pvmdr1 yang terbukti berhubungan dengan P. vivax resisten klorokuin di Papua. Dalam rangka kebijakan penggunaan Dihidroartemisinin-Piperakuin untuk pengobatan malaria vivaks di seluruh Indonesia, perlu data penyebaran parasit SNP Y976F pada gen pvmdr1 di luar Papua. Deteksi SNP Y976F dilakukan terhadap 95 sampel H0 subyek terinfeksi P. vivax atau infeksi campuran P.vivax dan P.falciparum yang telah dikonfirmasi dengan PCR. Hasil menunjukkan bahwa 88 dari 95 sampel (93%)  terdeteksi positif galur mutan 976F yang tersebar di  Kalimantan Barat (2 dari 3), Kalimantan Tengah (6 dari 8), Sulawesi Utara (63 dari 65) dan Sulawesi Tengah (17 dari 19). Kesimpulannya bahwa P.vivax galur Y976F sudah tersebar di setiap sentinel penelitian.   Kata kunci: P.vivax, pvmdr1, Single Nucleotide Polymorphism
Sebaran Serotipe Virus Dengue di Pontianak, Medan dan Jakarta Tahun 2008 Herman, Reni; Utami, Basundari Sri; Tuti, Sekar; Novriani, Harly
Jurnal Biotek Medisiana Indonesia Vol 1, No 2 (2012)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.245 KB)

Abstract

The severity of clinical manifestation of dengue infection depends on the 4 virus serotypes. The aim of the study is to explore the distribution of dengue virus serotypes in 3 cities (Pontianak, Medan and Jakarta) in 2008.The study was a cross-sectional designed; data were obtained from each 2 hospitals in Pontianak, Medan and Jakarta. Sera samples were collected from patients visiting internal and pediatric units in the hospital, which met the inclusion criteria, i.e. had fever for 2 – 7 days, with hemorrhagic manifestation, and/or thrombocyte level less than 100.00/mm3, haematocrit >20% , and had informed-consent signed. Sample sizes were 90 patients from each hospital. About 5 mL blood samples were collected, and serum were separated for RT-PCR testing to determine virus serotype. About 244 sera were collected, i.e. 95 sera from Pontianak, 86 sera from Medan, and 65 sera from Jakarta. Patients visiting hospital mostly had fever for 4 days. More than 60% of RT-PCR tested sera were dengue positive; with the serotype composition Den-3, Den-2 and 3, Den-3 and 1, respectively in Pontianak, Medan and Jakarta.Four dengue virus serotype circulated in Pontianak, Medan and Jakarta, with the majority of serotype 3 (Den-3) in Pontianak and Jakarta, and serotype 2 (Den-2) in Medan.Key words: DHF, dengue, serotype AbstrakDi Indonesia penyakit Demam Berdarah Dengue (DBD) sampai saat ini masih merupakan salah satu masalah kesehatan masyarakat, terutama di kota-kota besar. Tujuan dari penelitian ini adalah untuk mengetahui sebaran serotipe virus dengue tahun 2008 di kota Pontianak, Medan dan Jakarta. Desain penelitian potong lintang. Data yang dikumpulkan masing-masing dari 2 Rumah Sakit di Medan, Pontianak dan Jakarta. Sampel sera dikumpulkan dari penderita yang datang kebagian Penyakit Dalam dan bagian Anak, kriteria demam 2 sampai 7 hari, dengan manifestasi perdarahan dan atau trombosit < 100.000/mm3, Hematokrit> 20% nilai normal serta menandatangani informed concent. Jumlah sampel dari masing-masing Rumah Sakit 90 sampel. Darah penderita diambil maksimal 5 ml, serum dipisahkan dan dilakukan pemeriksaan RT-PCR untuk menentukan serotype virus. Sampel yang terkumpul pada penelitian ini 244, 95 sera dari Pontianak, 86 sera dari Medan dan 65 sera dari Jakarta. Penderita datang ke Rumah Sakit terbanyak setelah 4 hari demam. Dari Hasil pemeriksaan RT-PCR, lebih dari 60% positif, dengan komposisi serotipe terbanyak di Pontianak dengue 3, di Medan dengue 2, diikuti dengue 3 dan di Jakarta dengue 3 diikuti dengue 1.Dari hasil penelitian ini disimpulkan bahwa keempat serotipe virus dengue bersirkulasi di Pontianak, Medan dan Jakarta, dengan mayoritas virus dengue serotipe 3 di Pontianak dan Jakarta, serotipe 2 di Medan.Kata kunci:DBD, dengue, serotype
DETEKSI DAN SPESIASI PARASIT MALARIA SAMPEL MONITORING PENGOBATAN DIHYDROARTEMISININ-PIPERAQUINE DI KALIMANTAN DAN SULAWESI: MIKROSKOPIS VS POLYMERASE CHAIN REACTION Herman, Reni; Ariyanti, Endah; Salwati, Ervi; -, Delima; Tjitra, Emiliana
Media Penelitian dan Pengembangan Kesehatan Vol 21, No 3 Sept (2011)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v21i3 Sept.91.

Abstract

In monitoring the treatment of malaria with Dihydroartemisinin-piperaquine (DHP), microscopic cross check and Polymerase Chain Reaction (PCR) performed to validate the results of laboratory examinations in the field. This study used finger prick samples from subjects with a diagnosis of malaria in monitoring the treatment of malaria with DHP in Kalimantan and Sulawesi. Samples taken at day 0, blood smears made on slides for microscopic and blood spot on filter paper for PCR examination. The PCR method used is a single-round multiplex polymerase chain reaction that has been modified, the examination of each species carried out in different tubes to distinguish the species P. falciparum or P. Vivax. Target of DNA amplification is a species-specific gene sequences in the small-subunit ribosomal RNA (SSUrRNA), 300 bp for P. falciparum and 276 bp for P.vivax.  P. falciparum and P.vivax identified in 229 samples of blood smears and blood spots. Microscopic and PCR gave the same results, positive 93.4% and negative 6.6% with a sensitivity of  99% and specificity 93.3%. P.falciparum sensitivity and specificity of 92% and 99%, P.vivax 97% and 94%, PCR as a gold standard. There are differences in the results of examination of 5 samples, ie with microscopic examination identified as P.vivax  while the PCR as P. falciparum. In this study, identification of  the microscopic parasite similar to the results of identification by PCR, but differ in determining the types of parasites. In general, the ability to microscopic diagnosis of malaria is very good, but confirmation by PCR is still needed.AbstrakPada monitoring pengobatan malaria  dengan Dihydroartemisinin-piperaquine (DHP),cek silang mikroskopis dan Polymerase Chain Reaction (PCR) dilakukan untuk memvalidasi hasil pemeriksaan di laboratorium lapangan. Penelitian ini menggunakan sediaan darah jari dari subyek dengan diagnosis malaria pada monitoring pengobatan malaria dengan DHP di Kalimantan dan Sulawesi. Sampel diambil pada hari 0, dibuat sediaan apus darah pada kaca benda dan sediaan tetes darah (Blood spot) pada kertas saring. Terhadap sediaan apus darah dilakukan pemeriksaan mikroskopis, dan terhadap sediaan tetes darah dilakukan pemeriksaan PCR. Metode PCR yang digunakan adalah multiplex single round Polymerase Chain Reaction yang telah dimodifikasi, pemeriksaan masing-masing spesies dilakukan pada tabung yang berbeda untuk membedakan spesies P.falciparum atau P. Vivax. Target amplifikasi DNA adalah gen species-specific sequences pada small-subunit ribosomal RNA (SSUrRNA), 300 bp untuk P.falciparum dan P.vivax. P.falciparum dan P.vivax diidentifikasi pada 229 sampel berupa sediaan apus darah pada kaca benda dan blood spot. Hasil identifikasi dengan mikroskopis dan PCR, sampel positif 93,4% dan negatif 6,6% dengan  sensitifitas 99% dan spesifisitas 93,3%. Sensitifitas dan spesifisitas P.falciparum adalah 92% dan 99%, P.vivax 97% dan 94%, dihitung dengan PCR sebagai baku standar. Terdapat perbedaan hasil pemeriksaan terhadap 5 sampel, yaitu dengan pemeriksaan mikroskopis diidentifikasi sebagai P.vivax sementara pada pemeriksaan PCR sebagai P.falciparum. Pada penelitian ini, hasil identifikasi parasit dengan mikroskopis sama dengan hasil identifikasi dengan PCR, namun berbeda pada penentuan jenis parasit. Secara umum kemampuan tenaga mikroskopis pusat untuk menegakkan diagnosis malaria sudah sangat baik, namun untuk penentuan jenis Plasmodium masih memerlukan konfirmasi PCR.
Studi in Silico Lima Senyawa Aktif Sebagai Penghambat Protein Virus Dengue Herman, Reni
Jurnal Kefarmasian Indonesia VOLUME 9, NOMOR 1, FEBRUARI 2019
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v9i1.1157

Abstract

Dengue infection is an endemic disease in the tropics and subtropics, caused by dengue virus (DENV) infection. Some compounds have been shown to have antiviral effects on some viruses. In silico study is conducted to predict the stability of natural ingredient compounds: artemisinin, catechin, mangiferin, epigallocatechin gallate (EGCG), and quercetin in their interactions with dengue virus proteins at molecular level. This study is carried out using the 2008 version of the Molecular Operating Environment (MOE) software. Ligands are ribavirin as antiviral control whereas artemisinin, mangiferin, EGCG, and quercetin with 3D mole format structures. The downloaded DENV protein with PDB document format is the DENV serotype 2 envelope protein with 1OKE code, non structural protein 3 (NS3) with 2VBC code and NS5 protein with 1L9K code. In silico test generally showed that catechin, mangiferin, EGCG, and quercetin had more stable docking ligands to DENV’s proteins. In particular, mangiferin had stable docking ligand to envelope proteins, NS3 (helicase and protease) and in NS5-methyltransferase compared to ribavirin. Catechin stabled on NS3-protease, EGCG on NS3 (helicase and protease) and quercetin on NS3-protease. Artemisinin had less stabled bonds than ribavirin. The results indicated that catechin, mangiferin, EGCG, and quercetin had potential inhibition to DENV proteins whereas mangiferin was the most potential compound to inhibit dengue virus protein targets.
Co-Authors Agustiningsih Agustiningsih Basundari Sri Utami Delima -, Delima Eka Pratiwi Emiliana Tjitra Endah Ariyanti Ervi Salwati Harly Novriani Kartika D. Puspa Ririn Ramadhany Sarwo Handayani Sekar Tuti Vivi Setiawaty