cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Is Fatimah
Contact Email
eksakta@uii.ac.id
Phone
+6282326298724
Journal Mail Official
eksakta@uii.ac.id
Editorial Address
Faculty of Mathematics and Natural Sciences Universitas Islam Indonesia Jl. Kaliurang Km 14, Ngaglik, Sleman, Yogyakarta, 55584
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
EKSAKTA: Journal of Sciences and Data Analysis
Published by Universitas Islam Indonesia
ISSN : 27160459     EISSN : 27209326     DOI : 10.20885
Core Subject : Science, Engineering,
Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology
Ekstakta is an interdisciplinary journal with the scope of mathematics and natural sciences that is published by Fakultas MIPA Universitas Islam Indonesia. All submitted papers should describe original, innovatory research, and modelling research indicating their basic idea for potential applications. The Journal particularly welcomes submissions that focus on the progress in the field of mathematics, statistics, chemistry, physics, biology and pharmaceutical sciences.
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 11 Documents
 1   2 
Search results for , issue "VOLUME 5, ISSUE 1, April 2024" : 11 Documents clear
Optimasi Prosedur Kultur H9C2 Kardiomioblas: Sebuah Pembelajaran untuk Membangun Model Studi In-vitro Kardiovaskular Fadhillah, Muhamad Rizqy; Arozal, Wawaimuli; Wibowo, Heri
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 1, April 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss1.art8

Abstract

Cardiovascular disease become one of the leading factors of death in the world. Thus, research is urgently needed to discover newer drugs or therapeutical agents and biological plausibility. The H9C2 cardiomyoblast originated from embryonic BDIX rat ventricular cells and was previously used in numerous in vitro studies because of its similar nature to cardiomyocytes. However, to our knowledge, there are still limited studies on the basic procedure for culturing H9C2 cardiomyoblast and arranging the best strategy to perform a suitable timeline. Here, we shared our experience in culturing the H9C2 cardiomyoblast, including harvesting and subculturing the cells. We also demonstrated the change of cell confluency, depending on the seeding number, serum concentration, and culture flask through days 1, 3, and 6, to determine their doubling-time population. H9C2 cardiomyoblasts’ doubling time is around 48-54 days with Mean±SD 2.38±0.41. However, seeding density, different culture flasks, and serum concentration have become independent factors in determining specific measures to harvest the cells for further experiments.

Page 2 of 2 | Total Record : 11

 1   2 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue VOLUME 6, ISSUE 2, October 2025 VOLUME 6, ISSUE 1, April 2025 VOLUME 5, ISSUE 2, October 2024 VOLUME 5, ISSUE 1, April 2024 VOLUME 4, ISSUE 2, August 2023 VOLUME 4, ISSUE 1, February 2023 VOLUME 3, ISSUE 2, August 2022 VOLUME 3, ISSUE 1, February 2022 VOLUME 2, ISSUE 2, August 2021 VOLUME 2, ISSUE 1, February 2021 VOLUME 1, ISSUE 2, August 2020 VOLUME 1, ISSUE 1, February 2020 VOLUME 19, ISSUE 2, August 2019 VOLUME 19, ISSUE 1, February 2019 VOLUME 18, ISSUE 2, August 2018 VOLUME 18, ISSUE 1, February 2018 VOLUME 17, ISSUE 2, August 2017 VOLUME 17, ISSUE 1, February 2017 VOLUME 16, ISSUE 2, August 2016 VOLUME 16, ISSUE 1, February 2016 VOLUME 15, ISSUE 1-2, 2015 VOLUME 14, ISSUE 2, August 2014 VOLUME 14, ISSUE 1, February 2014 VOLUME 13, ISSUE 1-2, August 2013 VOLUME 13, ISSUE 1, February 2012 VOLUME 12, ISSUE 2, August 2011 VOLUME 12, ISSUE 1, February 2011 VOLUME 11, ISSUE 2, August 2010 VOLUME 11, ISSUE 1, February 2010 More Issue