cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 11, No 1 (2015): April" : 5 Documents clear
Pembentukan Pustaka Genom, Resekuensing, dan Identifikasi SNP Berdasarkan Sekuen Genom Total Genotipe Kedelai Indonesia I Made Tasma; Dani Satyawan; Habib Rijzaani
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p7-16

Abstract

Resequencing of the soybean genome facilitates SNP marker discoveries useful for supporting the national soybean breedingprograms. The objectives of the present study were to construct soybean genomic libraries, to resequence the whole genome offive Indonesian soybean genotypes, and to identify SNPs based on the resequence data. The studies consisted of genomiclibrary construction and quality analysis, resequencing the whole-genome of five soybean genotypes, and genome-wide SNPidentification based on alignment of the resequence data with reference sequence, Williams 82. The five Indonesian soybeangenotypes were Tambora, Grobogan, B3293, Malabar, and Davros. The results showed that soybean genomic library wassuccessfully constructed having the size of 400 bp with library concentrations range from 21.2–64.5 ng/μl. Resequencing of thelibraries resulted in 50.1 x 109 bp total genomic sequence. The quality of genomic library and sequence data resulted from thisstudy was high as indicated by Q score of 88.6% with low sequencing error of only 0.97%. Bioinformatic analysis resulted in atotal of 2,597,286 SNPs, 257,598 insertions, and 202,157 deletions. Of the total SNPs identified, only 95,207 SNPs (2.15%) werelocated within exons. Among those, 49,926 SNPs caused missense mutation and 1,535 SNPs caused nonsense mutation. SNPsresulted from this study upon verification will be very useful for genome-wide SNP chip development of the soybean genome toaccelerate breeding program of the soybean.
Segregation Analysis of SSR, SNP, and AFLP Markers in F2 Population of Solanum lycopersicum × S. arcanum (Analisis Segregasi Marka SSR, SNP, dan AFLP pada Populasi F2 Persilangan Solanum lycopersicum × S. arcanum) Chaerani Chaerani; R. E. Voorrips
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p1-6

Abstract

Distorted marker segregation is a common phenomenon in interspecific cross of various crops. Previous mapping study of earlyblight fungus (Alternaria solani) resistance loci showed 52% marker distortion in the genetic linkage map of 176 F2 progeniesderived from Solanum lycopersicum cv. Solentos × S. arcanum LA2157. The objectives of this study were to analyze in detail themarker segregation in the map and to determine the cause of segregation distortion by calculating the allele and genotypefrequencies of each marker. Out of 371 mapped markers, 192 markers deviated from the expected Mendelian ratio of 1 : 2 : 1.Distorted markers occurred in all chromosomes, ranging from 1% to 92%. Surplus of S. arcanum homozygotes contributed mostto the skewness (40%), followed by heterozygotes (18%), and S. lycopersicum homozygotes (5%). The allele frequencies of 152markers deviated from the expected allele homogeneity frequency, indicating that their segregation might be affected bygamethophytic selection. Sixty-one markers deviated from the expected F2 genotype frequency distribution, indicating that theirsegregation might be influenced by zygotic selection. Thirty-seven of the distorted markers showed deviation from expectedfrequencies of allele homogeneity and F2 genotype frequency distribution. Distorted markers can be retained in linkage analysissince chromosomal regions containing distorted markers showed linkage with early blight fungus resistance loci. Furtheridentification of the mechanism contributing segregation distortion requires detailed and extensive mapping studies.
Induksi dan Regenerasi Kalus Jagung yang Ditransformasi dengan Gen CsNitr1-L melalui Penembakan Partikel A. Dinar Ambarwati; Edy Listanto; Slamet Slamet; Umar Umar; Sustiprijatno Sustiprijatno; Sutoro Sutoro
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p25-32

Abstract

The success in development of transgenic plants is influenced by the regeneration system. The objective of the study was toassess the response of maize genotypes to regeneration system of organogenesis and embryogenesis, after transformed withCsNitr1-L gene through particle bombardment. Induction and callus regeneration of maize immature embryos of inbred linesUlt:cm.1#, ARC 178-123-112-XB3, and AZ2 were conducted through organogenesis, whereas those inbred lines AZ1, AZ2,P4G19(S)C2.59.3.3.1.3 and P4S3.29.4.4.1 were conducted through embryogenesis somatic. Transformation of CsNitr1-L gene wasdone with the distance of bombardment of 7 cm and 9 cm and calli were then selected using 10 mg/l hygromycin. All explants(100%) of inbred lines Ult:cm.1# formed organogenic callus, while callus formation of ARC 178-123-112-XB3 was 94.3% and AZ2was 60.5%. Ult:cm.1# was the most responsive line to the regeneration of organogenesis and produced 24 green shoots,compared with ARC 178-123-112-XB3 which produced one green shoot and AZ2 that did not produce green shoots. The highestpercentage of embryogenic calli formed through somatic embryogenesis was obtained on inbred lines AZ1 (85.4%) and thelowest was on P4S3.29.4.4.1 (18.9%). Inbred lines AZ1 had the highest percentage of regeneration (50.7%) and produced 62plants, followed by P4G19(S)C2.59.3.3.1.3 that produced 17 plants (2.8%) and P4S3.29.4.4.1 which produced two plants.Preliminary identification on 31 putative transgenic plants through PCR analysis produced 22 plants (70.96%) that contained nptIIgene.
Analisis Molekuler dan Keragaan Agronomis Galur-galur Padi BC1F1 Persilangan Code x qTSN4 dan Code x qDTH8 (Molecular Analysis and Agronomic Performance of BC1F1 Crosses Code x qTSN4 and Code x qDTH8) Tasliah Tasliah; Ma'sumah Ma'sumah; Kurniawan R. Trijatmiko; Joko Prasetiyono
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p17-24

Abstract

Breeding based on molecular marker has become a routine activity in the current rice research. The development of an earlymaturity of rice variety with high yield is needed to increase national rice production. This study aimed to determine the patternof alleles for loci controlling total spikelet number and number of days to heading, as well as agronomic performances of theBC1F1 Code x qTSN4 and Code x qDTH8 populations. The study was conducted at the Indonesian Center for Biotechnology andGenetic Resources Research and Development from January to August 2014. The plant materials used were Code (a nationalvariety with bacterial blight resistance gene [Xa7]), IR64-Nils-qTSN4[YP9] (qTSN4 that contains a locus controlling the number ofspikelet), IR64-Nils-qDTH8[YP1] (qDTH8 that contains a locus controlling the number of days to heading), BC1F1 Code x qTSN4,and BC1F1 Code x qDTH8. A total of 250 BC1F1 plants of each crosses were selected using molecular markers of RM20582 for Xa7gene, RM17483 and RM6909 for QTL position of qTSN4, RM5556 and RM6838 for QTL position of qDTH8. Based on molecularanalysis, there were 63 BC1F1-qTSN4 lines and 65 BC1F1-qDTH8 lines showing heterozygote alleles for qTSN4 or qDTH8 loci andwere homozygote for Xa7 locus (HHA pattern). Five plants from each locus target were backcrossed to the recurrent parent,Code, to obtain BC2F1 seeds. The remaining BC1F1 plants were self-pollinated to obtain BC1F2 seeds. Observations on someagronomic characters demontrated that the BC1F1 plants showed higher yield potential than Code and the flowering time of theBC1F1 progenis were also earlier than Code. These results indicated that the yield potential of Code could be improved byintrogression of qTSN4 and qDTH8 loci into the Code genome.
Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik Saptowo J Pardal; Slamet Slamet; Ragapadmi Purnamaningsih; Endang G. Lestari; Sutini Sutini
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p33-40

Abstract

The development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can beproduced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormoneapplication, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carriedout by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation.Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selectedas the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected linescontained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaMgene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratoryand Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were usedas control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaMgene.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2015 2015


Reset
Filter By Issues
All Issue Vol 17, No 2 (2021): DESEMBER Vol 17, No 1 (2021): JUNE Vol 16, No 2 (2020): December Vol 16, No 1 (2020): June Vol 15, No 2 (2019): December Vol 15, No 1 (2019): June Vol 14, No 2 (2018): December Vol 14, No 1 (2018): June Vol 14, No 1 (2018): June Vol 13, No 2 (2017): Desember Vol 13, No 1 (2017): Juni Vol 12, No 2 (2016): Desember Vol 12, No 1 (2016): Juni Vol 11, No 3 (2015): Desember Vol 11, No 2 (2015): Agustus Vol 11, No 1 (2015): April Vol 10, No 3 (2014): Desember Vol 10, No 2 (2014): Agustus Vol 10, No 1 (2014): April Vol 9, No 3 (2013): Desember Vol 9, No 2 (2013): Agustus Vol 9, No 1 (2013): April Vol 8, No 3 (2012): Desember Vol 8, No 2 (2012): Agustus Vol 8, No 1 (2012): April Vol 8, No 1 (2012): April Vol 7, No 2 (2011): Oktober Vol 7, No 1 (2011): Jurnal AgroBiogen Vol 7, No 1 (2011): April Vol 6, No 2 (2010): Oktober Vol 6, No 1 (2010): April Vol 5, No 2 (2009): Oktober Vol 5, No 1 (2009): April Vol 4, No 2 (2008): Oktober Vol 4, No 1 (2008): April Vol 3, No 2 (2007): Oktober Vol 3, No 1 (2007): April Vol 2, No 2 (2006): Oktober Vol 2, No 1 (2006): April Vol 1, No 2 (2005): Oktober Vol 1, No 1 (2005): April More Issue