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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 17, No 2 (2021): DESEMBER" : 5 Documents clear
Resistance Responses of 35 Watermelon Genotypes to Three Isolates of Fusarium oxysporum f. sp. niveum Nazly Aswani; Suryo Wiyono; Sobir Sobir
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p63-74

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is one of main diseases of watermelon. There have been very limited studies that tested watermelon genotypes to more than one isolates of Fon in Indonesia. This research aimed to determine the resistance of 35 watermelon genotypes to three Fon isolates taken from three different areas in Indonesia. Incubation period (IP) and disease index (DI) of the 35 watermelon genotypes were determined against three Fon isolates collected from Karawang (FK), Lampung (FL), and Purwakarta (FP). The experiment was arranged using a Completely Randomized Design with two replications. DI showed that six watermelon genotypes, i.e. New Hope, Sky Mountain, Southern Light, Super Sweet 66, Uranus, and Yellow Baby demonstrated moderate resistance to resistance phenotypes to all tested Fon isolates. IP and percentage of symptomatic plants (PSP) showed different responses among genotypes either to the same or to different isolates. Genotype New Orchid, for example, showed 57.14% symptomatic plants in less than 10 days after inoculation (DAI) when tested with FL isolate. Meanwhile, when tested with the same isolate, genotype New Dragon showed only 6.67% symptomatic plants in more than 23 DAI. The result of this study indicated that watermelon genotypes showing resistant to all tested isolates should be useful for breeding program to develop watermelon lines with broader resistance spectrum against Fon pathogen. The resistance genotypes selected should also demonstrate good agronomic performances and high yield to be considered as a new watermelon variety.
Genotypic Analysis of Two Local Swamp Rice Based on Microsatellite Markers Wage Ratna Rohaeni; Indrastuti A. Rumanti
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p75-82

Abstract

Argo is a local swamp rice from Kalimantan which is widely cultivated by local farmers. Exploration activities in Kalimantan found two types of local rice named Argo but demonstrated different phenotypes. Therefore, genotypic verification is needed to determine the differences between the two Argo rice genotypes. DNA fingerprint could help genotype verification rapidly and the result is not influenced by environmental factors. The aims of this study were to determine the level of similarity of Argo-1 and Argo-2 with swamp rice and other irrigated rice genotypes using microsatellite markers. A total of eight improved and local varieties were used as genetic material with the two local rice Argo (Argo-1 and Argo-2). The rice genotypes were analyzed by using eight microsatellite markers. The results of the molecular analysis showed that the two types of Argo showed different DNA bands based on the RM228 marker but genetically they were closely related. The genetic distance between the two Argo cultivars is 0.143. Argo-1 and Argo-2 were located in the same cluster as Inpara 4 and Siam KDK, but they were in the different groups from those of irrigated rice varieties such as Mekongga and Inpari 9. Based on this research, Argo-1 and Argo-2 are not recommended to be crossed with Inpara 4 and Siam KDK because the genetic diversity formed will be narrow.
Nucleotide Variations of Gamma Ray-Irradiated Chili Pepper Mutant Genotypes Based on Gene-Specific Primers Related to Fruit Size Character Nugroho, Kristianto; Trikoesoemaningtyas, Trikoesoemaningtyas; Syukur, Muhamad; Lestari, Puji
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p83-94

Abstract

Fruit size is a quantitative character controlled by several genes and mutations in those genes could lead to phenotypic changes in fruits. Single nucleotide polymorphism (SNP) is a molecular marker that could be used to detect mutation in genes at the nucleotide level that could not be done by conventional markers. The aim of this study was to analyze nucleotide variations, primarily SNP, in mutant chili pepper genotypes using gene-specific primers related to fruit size characters to measure the genetic changes occured, identify the SNPs that were associated with fruit size character, and analyze the phylogenetic among the mutant genotypes. Sanger sequencing was conducted on six mutant[K21]  chili pepper genotypes from M2 generation and Lingga as origin variety using five pairs of newly designed gene-specific primers corresponding to fruit size characters. A total of 41 base changes were identified in the gamma ray-irradiated chili pepper mutants, consisting of 22 insertions/deletions (indels) and 19 SNPs. As many as 17 nonsynonymous SNPs which caused amino acid changes, in contrast to the other 2 synonymous SNPs, were found in these mutant genotypes. Association analysis between identified SNPs to fruit length and diameter showed nonsignificant association, therefore the functional marker related to targeted gene could not be developed. The genotype L269 showed a separation from Lingga in dendrogram reflecting a large number of genetic changes, showed its potential as a new germplasm in development of improved variety in the future.
Molecular Analyses and Resistance Evaluation to Bacterial Leaf Blight on Swampy Rice Genotypes Ahmad Dadang; Siti Yuriah; Nonon Saribanon; Joko Prasetiyono; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p95-104

Abstract

Swampland is a prospective marginal land to be developed for rice cultivation. Rice plants that are adaptive to swampland usually have good tolerance to Fe. Previously, we obtained several rice lines tolerant to Fe toxicity in the field. This study aimed to analyze the swampy rice genotypes using STS molecular markers linked to bacterial leaf blight (BLB)-resistant genes and evaluate the resistance level of the genotypes against three BLB races under the greenhouse condition. This research was conducted in the Molecular Biology Laboratory and Greenhouse of the Indonesian Center for Agricultural Biotechnology andGenetic Resources Research and Development from January to May 2021. Eighteen swampy rice varieties/lines and two check varieties for Fe toxicity and BLB, respectively, were used in this research. The eight STS molecular markers linked to BLB resistance genes were used for genotyping analysis. The results showed that all rice plants tested contained xa5 allele gene, 19 genotypes had Xa4 allele gene, 17 genotypes had Xa7 allele gene, 9 genotypes had xa13 allele gene, 5 genotypes had xa13 allele gene, and 6 genotypes had Xa4 and Xa21 allele genes. Line B146E-4 was estimated to have 7 resistance alleles genes, meanwhile lines B14301E-KA-17-a, B14315E-KA-1, and B13-1-a have 6 resistance allele genes. The resistance evaluation of the genotypes against the three BLB races (races III, IV, and VIII) under the greenhouse condition showed that line B14315E-KA-1 was confirmed to be highly resistant to BLB disease. This line can be a promising candidate for swampy rice that is resistant toBLB disease.
Rapid Multiplication of Sugarcane Seedlings of PSJT 941 Variety from Tissue Culture Using Ethephon and Paclobutrazol Arinta Rury Puspitasari; Aprilia Mutia Dewi; Sri Winarsih
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p105-112

Abstract

Indonesia's sugar self-sufficiency was targeted since 2014 but the target has not been reached yet. One of the major constraints is the limited healthy seedlings available for new sugarcane area expansion. The mass production of healthy sugarcane seedlings may be achieved by rapid multiplication of tissue culture-derived seedlings using plant growth regulators (PGR) applied in the nursery house. The aim of this study was to determine the effect of ethephon and pacloburazol (PBZ) on the growth and seedling multiplication of sugarcane PSJT 941 variety. The research was carried out in the nursery house at Pasuruan Experimental Station, Indonesian Sugarcane Research Center. This study was arranged using a randomized block design with three replications. The treatments included (a) 3.5 ml/l ethephon, (b) 7.0 ml/l ethephon, (c) 1.5 ml/l PBZ, (d) 3.0 ml/l PBZ, and (e) control (without application of neither ethephon nor PBZ). The results showed that the highest number of shoots per clump was obtained from 3.0 ml/l PBZ treatment (8.7 shoots/clump) after 3 months of application. In the contrary, PBZ reduced the plant height compared with control and ethephon. Ethephon and PBZ treatments improved seed multiplication 8–52 times, much higher than the control that produced seed multiplication value six times. Further study is necessary to obtain optimal PBZ concentration for better stem height by decreasing the PBZ inhibition effect on stem growth.

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