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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 2, No 1 (2006): April" : 6 Documents clear
Regenerasi Tanaman pada Kultur Antera Beberapa Aksesi Padi Indica Toleran Aluminium Iswari S. Dewi; Bambang S. Purwoko; Hajrial Aswidinnoor; Ida H. Somantri; M. A. Chozin
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p30-35

Abstract

Anther culture provides the quick route in obtaining pure lines in a single generation from either green haploid plant that may be artificially or spontaneously doubled. Indica rice known as recalcitrant genotype because of its difficulty in regenerating sufficient number of green plantlets among the regenerated plants through anther culture. Whilst, research on studying anther culture ability has to be done to assure the success of rice breeding through anther culture. The objective of this research was to determine regeneration ability of five accessions of indica rice tolerance to aluminum through application of putrescine in anther culture. Completely randomized design with 15 replications was used in this research. Treatments consisted of five accessions of aluminum tolerance indica rice, ie. CT6510-24-1-3, Grogol, Hawara Bunar, Krowal, and Sigundil. Callus induction medium based on N6 medium + 10-3 M putrescine, while regeneration medium based on MS + 10-3 M putrescine. The results indicated that culture ability is controlled by the genotype. From this research, Grogol, Krowal and Sigundil were selected as accessions having good rice anther culture ability, and therefore can be used as parents for developing new rice varieties tolerance to aluminum through anther culture.
Pengaruh Cekaman Aluminium terhadap Kandungan Asam Organik dalam Kalus dan Pinak Tomat (Lycopersicon esculentum Mill.) Wening Enggarini; Erly Marwani
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p24-29

Abstract

The purpose of this research was to evaluate the effects of Al stress on citric, malic and oxalic acid content of L. esculentum cv. Intan callus and plantlet, also aluminum content of L. esculentum plantlet. Callus was induced from cotyledone of L. esculentum on Murashige & Skoog (MS) media containing 10-7 M NAA and 10-6 kinetin. The callus was then transferred step wisely at 3 weeks interval to media containing 220, 275, 330, 385, 440, 550, 825, and 1100 μM AlCl3. The callus cultures on the control media and media with the addition of 550 μM AlCl3 were able to regenerate and produce shoots after 8 passages of subculture. The shoots from media with the addition of 550 μM AlCl3 were transferred into the media with addition of 825 μM AlCl3, then to the media with 1100 μM AlCl3. The High Pressure Liquid Chromatography (HPLC) analysis showed that Al stress callus and plantlets contained malic acid, but no citric and oxalic acid. The content of malic acid in callus decreased with increasing AlCl3 concentration from 0 to 385 μM. On the other hand, the content of malic acid in callus increased with increasing AlCl3 concentration from 440 μM to 1100 μM. Similarly, the content of malic acid in root increased with increasing concentration of AlCl3 from 550 μM to 1100 μM. The result of Neutron Activation Analysis showed that Al content in root decreased as the amount of AlCl3 increased in the media. These results suggested that L. esculentum callus and plantlet respond to the Al stress by producing higher amount of malic acid.
Produksi dan Evaluasi Antibodi Poliklonal untuk Deteksi Toksin Photorhabdus spp. Yadi S Suryadi; Ifa S Manzila; Alina Thenawidjaya Akhdiya; Etty Pratiwi
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p16-23

Abstract

Production and Evaluation of Polyclonal Antibody forDetection of Photorhabdus spp. Toxin. Yadi Suryadi, IfaManzila, Alina Akhdiya, and Etty Pratiwi. The researchwas aimed to produce and evaluate polyclonal antibody(PAb) for specific Photorhabdus spp. bacterial toxin detection.Photorhabdus spp. toxin of HJ isolates which was purifiedusing Hi Prep. 16/60 Sephacryl S-200 HR column chromatographyrevealed three different peaks of polypeptides.The results showed that the protein concentration of crudeantigen protein (supernatant) was 3,711 μg/μl, whilst fractionof protein was 1,95 x 10-2 μg/μl, respectively. The bioassayusing Tenebrio molitor larvae-3 indicated that after 48 happlication, the percentage of larvae mortality by crude antigenwas lower (73%) than by fraction antigen (93%). Basedupon NCM-ELISA test, PAb of fraction protein derived fromHJ isolate reacted with Photorhabdus spp. antigen yieldedstronger or darker violet color on membrane than that ofcrude protein. In addition, it was observed that PAb coulddifferentiate specifically Photorhabdus spp. toxin with otherbacterial filtrate such as Xanthomonas oryzae pv oryzae, X.campestris pv glycinea, Ralstonia solanacearum, Pseudomonassyringae pv glycinea and P. fluorescens, however itshowed cross reaction with Escherichia coli. Further testsare needed in optimizing PAb-Photorhabdus spp. sensitivityto achieve effective concentration for detection of Photorhabdusspp. toxin as well as specificity test against otherbacterial antigens.
Analisis Sidik Jari DNA Plasma Nutfah Kedelai Menggunakan Markah SSR Santoso, Tri Joko; Utami, Dwinita Wikan; Septiningsih, Endang M.
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p1-7

Abstract

Accuracy is an important issue for plant germplasm identification, especially forvarietal conformation, registration, and plant protection. A study was conducted to determine genetic variation in 96 soybean accessions based on variation in size and number alelles using fluorescently-labeled SSR (Simple Sequence Repeat) markers on a capillary-electrophoresis DNA analyzer. This technology can be used to measure sizes of DNA fragments accurately and the genotyping protocol can be automated in a high-throughput manner. In addition, the germplasm as a whole can be further analyzed to measure the amount of genetic diversity and to identify agronomically-important genes or alleles for variety improvement. Results of the study indicated that nearly all the soyben accessions tested showed unique DNA fingerprints or genetic identities. The rare alleles (frequency <5%) that might have the potential in the variety improvement program had also been detected. Identification of the 96 soybean accessions using 10 SSR markers had detected 116 alleles, ranging between 7-19 alleles per locus, with the value of PIC (Polymorphism Information Content), reflecting the value of frequency and allele variation) 0.703. The tendency for clustering together of the allelles in certain groups of the improved soyben varieties indicating that there were close genetic relationships among them. In addition, molecular differences between two accessions having the same names but with different number of registrations were detected. Furthermore, the presence of two soybean accessions with different names but having the same molecular identity was also identified.
Status Perkembangan Perbaikan Sifat Genetik Padi Menggunakan Transformasi Agrobacterium Rahmawati, Syamsidah
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p36-44

Abstract

Genetic transformation of rice becomes an important research area in recent years. Rice is staple food for almost half of world population and has been extensively used as a plant model system for monocotyledonous plant. Compare to direct DNA transfer techniques (PEG, electroporation, and DNA bombardment), Agrobacterium tumefaciens-mediated transformation was considered to be more advantageous because it is easy to handle, integration and segregation pattern are more predictable, and the likelihood to get transgenic plant with low copy number is high, thus decreasing gene silencing phenomena. Various important genes have been introduced into rice genome via Agrobacterium transformation. A number of important factors affecting the Agrobacterium transformation and the application of this technique in the next future will be discussed.
Nodulation and Growth of a Supernodulating Soybean Mutant SS2-2 Symbiotically Associated with Bradyrhizobium japonicum Lestari, Puji; Van, Kyujung; Kim, Moon Young; Lee, Suk-Ha
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p8-15

Abstract

Mutan kedelai penghasil nodulsuper menunjukkan kelemahan dalam kontrol autoregulasi pada nodulasi dan perbedaan fenotip dibandingkan dengan tipe liarnya. Studi untuk mengevaluasi karakter pertumbuhan dan nodulasi dari kedelai penghasil nodulsuper dalam asosiasinya dengan Bradyrhizobium japonicum dilakukan dalam penelitian ini. Tiga genotip kedelai, yaitu mutan kedelai penghasil nodulsuper SS2-2, tipe liarnya Sinpaldalkong 2 dan kedelai kontrol Jangyeobkong diinokulasi dengan B. japonicum USDA 110, kemudian ditumbuhkan di rumah kaca dalam kondisi terkontrol. Karakter nodulasi, fiksasi nitrogen (Acetylene Reduction Activity/ARA), pertumbuhan tanaman, dan hasil biji ditentukan untuk mengevaluasi asosiasi simbiotik antara B. japonicum dan kedelai nodulsuper. Kedelai yang diinokulasi dengan B. japonicum menunjukkan peningkatan jumlah dan berat kering nodul serta berat kering total tanaman dibandingkan dengan tanpa inokulasi. Tanaman SS2-2 yang diinokulasi menunjukkan jumlah nodul sekitar 20 kali lipat lebih tinggi daripada tipe liarnya. Inokulasi B. japonicum ternyata juga meningkatkan fiksasi nitrogen seiring dengan perkembangan nodul. Tanaman S2-2 lebih pendek dan menghasilkan fiksasi nitrogen (ARA) lebih tinggi, tetapi spesifik ARA dan hasil biji lebih rendah dibandingkan dengan tipe liarnya. Berdasarkan hasil evaluasi terhadap nodulasi dan pertumbuhannya, interaksi Rhizobium dan kedelai penghasil nodulsuper SS2-2 mempunyai respon asosiasi simbiotik lebih rendah dibandingkan kedelai penghasil nodul normal (kedelai yang tidak mendapat perlakuan mutasi).

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