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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 7 Documents
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Search results for , issue "Vol 7, No 2 (2011): Oktober" : 7 Documents clear
Identifikasi Entomopatogen Bakteri Merah pada Wereng Batang Coklat (Nilaparvata lugens Stål.) Tri Puji Priyatno; Yohana A Dahliani; Yadi Suryadi; I Made Samudra; Dwi Ningsih Susilowati; Iman Rusmana; Baskoro S Wibowo; Cahyadi Irwan
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p85-95

Abstract

Indentification of Entomopathogenic Red Bacterial fromBrown Planthopper (Nilaparvata lugens Stål.). Tri P.Priyatno, Yohana A. Dahliani, Yadi Suryadi, I MadeSamudra, Dwi N. Susilowati, Iman Rusmana, Baskoro S.Wibowo, and Cahyadi Irwan. Red bacteria isolated frombrown planthopper (BPH) has been proven pathogenicagainst BPH and others insects. Application of 106 to 107cells/ml of red bacteria caused 65.6-78.2% mortality of BPH.The 50% effective concentration (EC50) and lethal time of redbacteria against BPH is 2.8 x 105 cells/ml and 6.8 days,respectively. Based on phenotypic characters tested on GNMicroPlateTM Biolog kit and 16S rRNA sequneces analysis,red bacteria was identified as Serratia marcescens with 99%similarity. Red pigmen produced by S. marcescens strainBPH is secondary metabolite determined as prodigiosinshowing bactericidal activities against Xanthomonas oryzaepv. oryzae. We concluded that S. marcescens did not onlypotent as biocontrol agent to BPH, but also it can be used tocontrol plant pathogenic bacteria.
Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro Deden Sukmadjaja; Ade Mulyana
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p106-118

Abstract

(Saccharum officinarum L.) through In Vitro Culture.Deden Sukmadjaja and Ade Mulyana. The research wasconducted at the Laboratory of Tissue Culture The Biology ofCell and Tissue Researcher Group ICABIOGRAD, Bogor fromJune to November 2009 to studied growth and regenerationsresponse some varieties of sugarcane through in vitroculture. The research activities have been carried out inthree steps, i.e., callus formation, regeneration of shoots androots regeneration. The type of explants used in the studywas in vitro planlet explants of both sugarcane varieties.Seven media formulations were used for the callus inductionand regeneration of shoots, while five media formulationswere used for the roots regeneration. The resultsshowed that the highest respond for calluses induction wasBulu Lawang varieties at media formulation MS + 2.4-D 2mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varietiesat media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1.While the highest respond for regeneration of shoots wasBulu Lawang varieties at media formulation MS0 (controlMS) dan PS 951 varieties at media formulation MS + BAP 1mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1.The highest respond of roots regeneration was Bulu Lawangand PS 951 varieties at media formulation MS + IBA 1 mg.l-1.Acclimatization of plantlets produced were grew successfullyabout 90-100% in greenhouse.
Keragaman Genetik 50 Aksesi Plasma Nutfah Kedelai Berdasarkan Sepuluh Penanda Mikrosatelit Chaerani Chaerani; Nurul Hidayatun; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p96-105

Abstract

Genetic Diversity of 50 Soybean Accessions Based on TenMicrosatellite Markers. Chaerani, Nurul Hidayatun, andDwinita W. Utami. Soybean accessions in germplasmcollection have increased in number as a result ofexploration, introduction as well as development or releaseof new commercial varieties. This complicates accurate andreliable evaluation of an accession for purposes of utilizationin breeding program and discrimination of a newcommercial variety for purposes of plant variety protection.The aims of this study were to identify the genetic diversityof soybean germplasm to complement the existingphenotypic database as the basis for efficient managementand accurate discrimination of commercial varieties, and toidentify potential parents for hybridizations. Fifty soybeanaccessions consisting of 12 released varieties, 32 localvarieties, and 6 introductions were analyzed usingmicrosatellite DNA markers based on semi-automatic sizingsystem. A total of 86 alleles were detected with the numberof alleles per locus ranged from 4 to 16. Rare alleles weredetected at a rate of 53% which was shown by 68% of thegenotypes. Informativeness of the microsatellite markers asmeasured by the average gene diversity (D) orpolymorphism information content (PIC) was 0.60 and 0.58,respectively. A heterozygosity level of 0.09 as detected byseven loci was observed among 64% of the genotypes. Theaverage genetic distance among the genotypes was 0.56,which indicated the relatively low polymorphism among theanalyzed soybean germplasm. Four microsatellites thatshowed a high D or PIC value (over 0.75) were able todiscriminate between accession reliably. Each soybeanaccession had different DNA microsatellite fingerprint whichcan be used for accurate discrimination to complement theprevious conventional characterizations. UPGMA clusteringseparated the 50 accessions into 10 major clusters, whichshowed no clear pattern of clustering according to varietalgroup or geographical origin. Genetic similarity dataidentified five clusters and 15 genotypes with highest interclusteror inter-genotype genetic distances which arepotential candidates to be exploited as parents inhybridizations for development of new commercial varieties.
Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293 I Made Tasma; Ahmad Warsun; Dani Satyawan; Saptowo Jumali Pardal; Slamet Slamet
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p69-75

Abstract

Genetic Mapping of SSR Markers in Eight SoybeanChromosomes Based on F2 Population B3462 x B3293. IMade Tasma, Ahmad Warsun, Dani Satyawan, SaptowoJ. Pardal, and Slamet. Aluminum toxicity is one of the maincontrains for cultivating soybean in acid soils. GeneticHak Cipta © 2011, BB-Biogenmapping of SSR markers is one step for detecting aluminumtoxicitytolerant QTLs in soybean. Another step is tophenotype the same population at various aluminum-toxicityenvironments. The objectives of this study were to analyzethe segregation of SSR markers in progenies of an F2population and map the markers in 8 soybean chromosomes.The F2 population was previously developed bycrossing the Al-tolerant parent B3462 and the Al-sensitiveparent B3293. Polymorphic SSR markers in the parents wereused to PCR amplify DNA of the 100 F2 progenies. PCRproducts were separated using agarose or polyacrylamidegels. A Chi-Square test was done with a null hypothesis thatprogenies segregated in a 1 : 2 : 1 ratio. Results showed that125 SSR markers were polymorphics in the parents. Out of125 polymorphic markers, 122 were segregated in theprogenies of the F2 population. Among the segregatingmarkers, 114 were segregated in a 1 : 2 : 1 ratio. Only 8markers (5.6%) did not follow the 1 : 2 : 1 ratio. One hundredand nineteen SSR markers were mapped in 8 soybeanchromosomes. These include 18 markers in chromosomeA2, 10 in B1, 16 (C1), 16 (F), 10 (G), 23 (J), 16 (L), and 10 (N).Total genetic maps covered was 1,194.8 cM with averagemap distances between two adjacent markers of 10.7 cM.Further SSR marker enrichment is required to fill in the gapsof several chromosomal regions. Genetic maps presented inthis study should be useful for detection of Al-toxicitytolerant QTLs in soybean.
Keragaman Genetik 96 Aksesi Plasma Nutfah Padi Berdasarkan 30 Marka SSR Terpaut Gen Pengatur Waktu Pembungaan (HD Genes) Utami, Dwinita Wikan; Sutoro, Sutoro; Hidayatun, Nurul; Risliawati, Andari; Hanarida, Ida
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p76-84

Abstract

Genetic Diversity of 96 Accession of Rice GermplasmUsing 30 SSR Markers Linked to Heading Date Genes (HDGenes). Dwinita W. Utami, Sutoro, Nurul Hidayatun,Andari Risliawati, and Ida Hanarida. Rice with earlymaturity is one of an important genetic resources in ricegermplasm collection. Characterization and identification ofgenetic diversity is an important issue for plant variety protection.Molecular identification by microsatellite markersusing Genetic Analyzer enables resolve of this issue. Theobjective of this research is to identify the genetic diversity of96 rice accessions based on their specific DNA fingerprintusing microsatellite markers. A total of 96 accessions consistingof a diverse variety of maturity classification weregenotyped with 30 SSR markers linked to HD genes whichspread out in 12 chromosomes of rice geneome. The total297 alleles were detected indicated the level of markerinformativeness. RM5607 generated 7 allele with the sizerange from 103 to 197 and the highest PIC at 0.90. RM3571(linked to HD12 gene) has a significant value associated withvarieties which have early maturity trait. Clustering analysisshowed the cluster based on Sub Species genome backgroundand on early maturity trait.
Plot Refugi untuk Pengelolaan Resistensi Hama terhadap Tanaman Transgenik Bt Bahagiawati Bahagiawati
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p128-137

Abstract

Refugea Plot as Insect Resistance Management inTransgenic Bt Crops. Bahagiawati. The objective of thisreview is to share information on several cases of targetinsects became resistance to transgenic-Bt crops in the fieldand the refuge strategy used to manage this problem. Btcorn and Bt cotton have been planted widely for severalyears globally. One of the risks of planting transgenic Bt cropis the ability of the target insects adapted to the Bt proteinand caused the resistance breakdown the transgenic Btplants. This phenomenon was hypothesized in early 1990sbased on the cases of several insects resistance to microbialBt sprayed in laboratories and in the field. The mode ofaction of the pest resistance to Bt-toxin have been studied inseveral laboratories. In USA, to avoid the target insectresistance to transgenic Bt crops, a program called InsectResistance Management (IRM) has been applied since 2001for farmers growing Bt crops. Lately, there have been somereports of target insects became resistance to cry1F, cry1Ab,and cry1Ac in transgenic Bt crops. A report informed aboutthe resistance of target insect in Puerto Rico was publishedin 2006, and so in South Africa in 2006/2007, and the last onein India in 2009. To avoid target’s insect become resistanceto Bt crops, a program called structural IRM and unstructuralIRM were introduced and applied in severalcountries. One of the components of IRM is planting refugeplot, a plot that planting with isogenic line of Bt crops in/nearby the area of Bt crops. This review will discuss about thecases of target insect became resistance to Bt crops in thefield, mode of action of insect resistance to Bt, the model ofIRM program in USA and the Philippines and finally therecommendation for Indonesia to prepare its IRM programfor implementing Bt crops.
Sidik Jari DNA 88 Plasma Nutfah Ubi Jalar di Indonesia Berdasarkan Delapan Penanda SSR Nurul Hidayatun; Chaerani Chaerani; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p119-127

Abstract

DNA Fingerprinting of Indonesian 88 Sweet PotatoGermplasm Based on Eight SSR Markers. NurulHidayatun, Chaerani, and Dwinita W. Utami. Indonesiapossesses a great number of sweet potato varieties.Understanding the diversity and distribution of this geneticresource is essential for its management and future use. Theobjective of this study was to elaborate the molecularcharacter as DNA finger print of Indonesian sweet potatogermplasm. Eight fluorescent labeled SSR primers wereused to amplify DNA of 88 sweet potato accessionsconsisting of improved varieties and landraces collectedfrom 7 islands in Indonesia. The amplified products weredetected using capillary electrophoresis method in CEQGenetic Analysis System machine. A total of 135 allelesranging from 8 to 36 alleles per locus with an average of 17alleles were generated. Each accession had a uniquemicrosatellite finger print marked by specific combination of11 to 22 alleles in 8 SSR loci. Dendrogram generated byUPGMA based on simple matching coefficients produced 4nonspecific groups at 80% similarity. The groups revealedthe possibilities that the accessions were distributed fromsimilar genetic resources.

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