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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
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Search results for , issue "Vol 8, No 3 (2012): Desember" : 5 Documents clear
Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung Iswari S Dewi; Anggi Nindita; Bambang S. Purwoko; Darda Efendi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p89-96

Abstract

Propagation through tissue culture of plantspecies with rich secondary metabolites such as Jatrophacurcas L. is difficult to obtain. However, once established, itcan be used as one of the alternatives to supply uniformpropagules. The effects of auxin and cytokinin on theregulation of de novo woody plants shoot development havebeen studied through shoot induction, differentiation anddevelopment. The objective of this research was to identifyexplant and suitable culture media for in vitro shoot inductionthrough indirect organogenesis. Factorial experimentwas arranged in a completely randomized design, replicated20 times. The first factor was explants, i.e. cotyledons andhypocotyls. The second factor was MS media containingcombination of plant growth regulator IAA (0, 0.05, and 0.1mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of theexperiment showed that the fastest callus initiation wasachieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explantswere cultured. Shoots with leaves can be induced from bothcotyledons and hypocotyls. However, hypocotyls gave moreshoots and leaves than cotyledons when cultured in MS +IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotylsand cotyledons were successfully rooted in MS mediumwithout any growth regulator.
Perbanyakan Tanaman Jambu Mete (Anacardium occidentale L.) melalui Jalur Organogenesis Rossa Yunita; Ika Mariska; Christiani Tumilisar
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p113-119

Abstract

Vegetativepropagation through in vitro culture has been carried out asa technology that has the potential for obtaining seedling insignificant amounts and relatively faster. This activity can bedone through the multiplication of adventitious shoots andlateral shoots (organogenesis). The goal of this research wasto find the method of cashew micropropagation throughorganogenesis. This study consisted of 4 main activities.They were shoot induction, shoot multiplication, shootelongation, and root induction. The results showed the bestmedium composition for shoot induction was MS + BA 0.7mg/l. The suitable media for shoots multliplication was MS +thidiazuron 0.5 mg/l + zeatin 1 mg/l and for shootselongation was MS + GA 1 mg/l + zeatin + 3 mg/l. The bestmethods for root induction was by submerging in vitroshoots in a solution of IAA 100 mg/l.
Pemetaan, Karakterisasi, dan Pengembangan Primer-primer Lokus Pup1 (P uptake 1) pada Padi untuk Peningkatan Toleransi terhadap Defisiensi Fosfor Joko Prasetiyono; Tasliah Tasliah
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p120-129

Abstract

Phosphorus (P) is the second most important nutrient forplants after nitrogen, but is available in very low amount. Pdeficiency in rice would reduce the number of tillers andgrain production. There are numerous publications onexploration of genes that are associated with P. Manyresearches on P that are directed to breeding program andinvolving many countries/institutions focus on Pup1research. Pup1 (P uptake 1) is associated with P uptake hasbeen well mapped on chromosome 12 at a distance of 15.31to 15.47 Mb and microsatellite markers between RM28073and RM28102 can be used as a selection tool in the MAB(Marker Assisted Backrossing) program. Indonesia is veryconcerned with this research because of P-deficientproblem. This review aims to provide current information ofresearch that explore the genes in Pup1 locus. This reviewoutlines the history of Pup1 mapping, to explain sequenceand expression analysis of Pup1, and to inform of Pup1specific primers. The latest information is expected to beuseful for rice breeders in Indonesia, especially for thosewho are interested to P deficiency research. Study of geneswithin Pup1 locus is still ongoing, and found that somegenes do not contribute directly to P uptake. This mayindicate that Pup1 locus use other mechanisms in the Puptake. This may indicate that some genes (dirigent-like,fatty acid α-dioxygenase, aspartic proteinases) play a role inthe increasing level of lignin in P deficient condition.Increasing level of lignin would increase the volume of rootsand thus increasing P uptake and resistance to biotic andabiotic stresses. Specific markers to detect the genes in thePup1 locus have been successfully developed, and can beused for breeding and exploration activities on Indonesianrice germplasm.
Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot) Nurwita Dewi; Bambang S. Purwoko; Ida Hanarida; Agus Purwito; Iswari S. Dewi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p105-112

Abstract

Taro is a potential source of carbohydratefor anticipation of climate change. In vitro technology havenot been widely implemented for tuber crops conservation.Conservation of the crops is mostly conducted in field. Suchconservation is very susceptible to biotic and abiotic stress.The research consisted of two activities i.e: micropropagationand conservation. The objectives were to obtain taro invitro propagation and conservation method. The trial wasarranged in a factorial design with six replications. Five taroaccessions were used as the first factor for each study. Thesecond factor in propagation study was propagation mediumi.e: MS; MS + 2.9 μM IAA + 4.4 μM BA and MS + 2.9 μM IAA+ 22,2 μM BA. Shoot tip from taro sucker was used asexplant. The second factor in conservation study was MSmedium containing mannitol (0, 30, 40, and 50 g/l). Twoleavesin vitro shoots from micropropagation study was usedas explants. The addition of BA in MS medium + 2.9 μM IAAincreased the number of shoot of taro germplasm. The bestmedium for micropropagation of taro germplasm No. 21 andTalas Jahe is MS + 2,9 μM IAA + 4,4 μM BA, whereas thebest medium for No. 503, Talas Jahe and Lumbu Banten isMS + 2,9 μM IAA + 22,2 μM BA. Based on data of plantheight, percentage of leaf life and shelf life, MS medium +manitol 40 g/l was the best medium for taro germplasmconservation with prolong sub-culture interval.
Transformasi Genetik Pisang Ambon dengan Gen Kitinase dari Padi Ragapadmi Purnamaningsih; Deden Sukmadjaja
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p97-104

Abstract

One of the main constrains on theproductivity and quality enhancement of banana is wiltdiseases caused by Fusarium oxysporum (Foc). Productiondecrease by wilt disease was 63.33%. Therefore, an effort toobtain the banana new variety which is tolerant to fusariumwas absolutely necessary to be done. Genetic engineeringcan be used in new variety improvement, especially forproduction of pest and disease tolerant varieties.Transformation of banana with chi gene which expressedchitinase enzyme have been used in obtaining the plantresistant to Foc. The goals of the research were to obtain:determine lowest higromisin consentration inhibited nodulgrowth by tested four consentration of higromisin,determine optimum cocultivation time by tested three timescocultivation, tested asetosiringone added on two timescocultivation, and gen chi introduction at bananatransforman shoots with PCR. The explants used werenodule induced from pseudostem of banana cv. Ambonkuning. Genetic transformation done by sowing the explantsin bacterial suspension 0, 15, 30, and 45 minutes.The effectof asetosiringone (0 and 100 mg/l) on cocultivation mediumwas observed. The research results showed that the lowesthigromisin concentration inhibited nodule growth was 25mg/l for 5 weeks and the best time for inoculation of nodulewere 30 minute. Asetosiringone added on bacterialsuspension did not increase transformation efficiency.Chitinase gene transformation using Agrobacteriumtumefaciens on banana nodules produced 25 noduly ines ofputative transformant on selection media and 34 plantstransforman identification by PCR.

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