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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 10 Documents
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Search results for , issue "Vol 16, No 1 (2010): June" : 10 Documents clear
Marka Mikrosatelit sebagai Alternatif Uji BUSS dalam Perlindungan Varietas Tanamam Padi Sugiono Moeljopawiro
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p1-7

Abstract

This study was conducted at theIndonesian Center for Agricultural Biotechnology and GeneticResources Research and Development to seek the feasibility ofusing SSR markers for DUS testing in rice variety. Releasedvariety Fatmawati together with seven other reference varietieswere analyzed using genetic analyzer for their variability, tenSSR markers were used. Hundred and twenty five alleleslocated on chromosomes 1, 2, 5, 6, 7, 10, and 11 were detectedin eight varieties studied, where the number of alleles rangingfrom 6 (BPC) to 33 (Fatmawati), with the predominantmarkers such as RM11 on Fatmawati, Maros, Barumun,Gilirang, and Memberamo varieties, RM237 on Cisadane andBP630 varieties, and RM133 and RM287 markers on BPCvariety. The genetic distance-based results in the unrootedneighbor-joining tree revealed that the eight varieties as well astheir populations clustered separately. Genetic variabilitywithin each variety indicated that these varieties were still inhigher degree of heterogeneity. This demonstrated the powerof SSR marker in differentiating varieties as well as populationwithin variety, which, therefore, SSR marker could berecommended in plant variety protection.
Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir Imron Riyadi
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p57-63

Abstract

Thekind and concentration of enzyme that appropriate affectedisolation process and result of plant protoplast. The researchwas conducted to enhance the protoplast rendements of wingedbean (Psophocarpus tetragonolobus L.) that was isolated byCellulase RS and Macerozyme R-10 enzyme as single and itscombination in a solution. Concentrations of enzyme usedwere as much as 2.0-3.0% w/v for Cellulase RS and 0.4-0.6%w/v for Macerozyme R-10. The solution containt mannitol 25mM as an osmotycum. Isolation process was done on shakerwith 50 rpm (rotation per minute) speed in dark room for 5hours. Results showed that C3 treatment (concentration ofCellulase RS enzyme as much as 3.0% w/v) resultedprotoplasts density 3.49 x 105 protoplasts/g fresh weight ofhypocotyl and M2 treatment (concentration of Macerozyme R-10 enzyme as much as 0.5% w/v) resulted 3.19 x 105protoplasts/g. Whereas the best combined enzymes treatmentwas achieved by C3M2 (combination between Cellulase RS asmuch as 3.0% and Macerozyme R-10 enzyme as much as 0.5%w/v) which resulted protoplasts density 4.94 x 105protoplasts/g fresh weight of hypocotyl. The protoplast wasintact and viable.
Evaluasi Plasma Nutfah Kacang Tunggak (Vigna unguiculata L.) di Lahan Masam Mamik Setyowati; Sutoro Sutoro
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p44-48

Abstract

Plant genetic resources are as a source of geneticvariability and can be used to develop new varieties tolerant toabiotic and biotic stress. Evaluation of cowpea germplasm toabiotic stress, such as acidic soil has to be done to obtaininformation of their tolerance. Cowpea germplasm collectionheld in ICABIOGRAD was tested under acidic soil conditionin Jasinga, West Java and Bogor as control during March-June2007. The criteria of tolerance to acidic soil was determinedwhen the grain yield of cowpea under acidic soil more than80% compare to those yield under non acidic soil, whilesusceptible to acidic soil when lower than 25%. Result ofexperiment showed that accession of LO-3-38, Ces-41-6,TVX-4661-01D-A, IT82-889-A, TV.3381-0-2j-B and KacangTolo Loreng were tolerant to acidic soil, while Kacang Dadapdan Kacang Tolo were susceptible. These accessions could beused as parent materials for genetic study related to acidic soilstress.
Karakterisasi Beberapa Sifat Kualitatif dan Kuantitatif Plasma Nutfah Ubi Kayu (Manihot esculenta Crantz.) Nani Zuraida
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p49-56

Abstract

Utilization of cassava plant genetics resourcescould be done when the agronomical and morphologicalcharacters are available. Qualitative and quantitative of plantcharacters have been studied during April-December 2006 atCikeumeuh Experimental Station, Bogor. A total of 225accessions cassava germplasm were planted in 2 m x 3 m plotsize with 100 cm x 60 cm plant spacing. The plants werefertilized by 60 kg N, 20 kg P2O5, and 90 kg K2O per ha. Onethird N and K fertilizers, and all of P were applied at plantingtime, while two third N and K were applied at 3 months afterplanting. The result showed that coefficient variation ofqualitative and quantitative characters data of cassavaaccessions collected showed high variation. Therefore theaccessions are going to be maintained and conserved forgenetic resources. The plant height, number of tuber, greenmatter weight, and harvest index were positive correlation withtuber weight. While stem diameter and character of leaf lobedid not correlate with tuber weight. Among qualitativecharacters (the colors of young leaf, leaf vein, leaf stalk length,leaf lobe, stem, tuber, and number of leaf lobe) did not showdifference on tuber weight.
Evaluasi Toleransi Plasma Nutfah Padi terhadap P Rendah di Tanah Sawah Abd. Aziz Syarif; Didy Sopandie; M. A. Chozin; K. Idris; Suwarno Suwarno
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p8-16

Abstract

A field experiment was conducted to assessgenotypic variability of tolerance and to identify tolerantgenotypes to low phosphorus (P) among rice germplasm onlowland (paddy) rice field with low available and potential Pusing strip plot design with three replications. P fertilization(with and without added P) was assigned as horizontal factorand 120 rice plant genotypes as vertical factor. Data on plantheight, tiller number, and shoot dryweight were collected. LowP tolerance was judged by relative value i.e. observation valueat the plot without P treatment devided by the value at the plotwith P treatment. The judgement was determined based onIRRI standard. Shoot P uptake of five tolerant and fivesensitive genotypes on no added P treatment was alsoobserved. The results showed that the germplasm exhibitedphenotypic and genotypic variability of low P tolerance. Thehighest genotypic variability of tolerance was shown by thevalue based on relative tillering number (28.7%), followed bythat of relative shoot dryweight (22,6%) and relative plantheight (7,6%). The highest broadsense heritability of tolerancewas shown by the value based on relative tiller number(24.1%), followed by that of relative plant height (16.6%) andrelative shoot dry weight (15.0%) Fourteen genotypes wereidentified as highly tolerant based on relative tiller number and28 genotypes based on relative shoot dryweight. The toleranceof genotype was attained by higher P uptake and higherinternal use efficiency.
Identifikasi Sumber Ketahanan Aksesi Plasma Nutfah Kedelai untuk Ulat Grayak Spodoptera litura F. Suharsono Suharsono; M. Muchlish Adie
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p29-37

Abstract

The importantaspect of development of resistant plant to insect pest is sourceof resistance. Study the resistance of 14 advance soybeanbreeding lines to common cutworm Spodoptera litura F. wasconducted at the Laboratory of Crop Protection, IndonesianLegumes and Tuber Crops Research Institute (ILETRI)Malang in February-September, 2006. Leaf damage and larvaldevelopment on resistant genotypes was recorded to measurethe level of resistance. It was found that the susceptibility ofsoybeans to the common cutworm significantly varied amongthe breeding lines. The leaf damage of IAC-100, IAC 80-596-2, and W/80-2-4-20 from larval feeding were 17.67, 18.52, and23.70% respectively lower than Wilis variety with 35.57% ofleaf damage. These breeding lines consistently possess samelevel of resistance to S. litura. In addition, the resistantbreeding lines affect on biological aspects i.e. prolongedduration of larval stage, reduced larval and pupae gain weight,and cause significant larval mortality compared with Wilisvariety. The study suggested that IAC-100, IAC 80-596-2, andW/80-2-4-20 could be used as a source of resistance for S.litura in breeding program.
Kemampuan Berbunga, Tingkat Keguguran Bunga, dan Potensi Hasil Beberapa Varietas Kedelai Suyamto Suyamto; Musalamah Musalamah
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p38-43

Abstract

Field experiments toidentify the flowering ability, flowering duration, percentageof flower abortion, and yield potential of several soybeanvarieties were conducted at two environments at Kendalpayak-Malang Research Station in April-July 2006 (dry season I) andGenteng-Banyuwangi Research Station (dry season II) in July-October 2006. A total of 20 soybean varieties were evaluatedby using randomized complete-blocks design with threereplications. The plot size was 0.8 m x 4.5 m with plantspacing 40 cm x 15 cm. The rate of fertilizers applied was 50kg urea, 100 kg SP36, and 75 kg KCl per ha. Plant control wasconducted intensively as recommended. Several charactersobserved were time to flower, flowering duration, number offlower, number of pod, number of filled pod, number of emptypod, seed yield/plant, and 100 seed weight. Combined analysisof variance show that all characters oberved were significantlyaffected by varieties. Flower abortion of soybean varietiesrange from a low of 6.6% for Lawit to a high of 39.1% forKawi. Lawit variety was used as high yielding potential parentwhich has lowest percentage of flower abortion (6.6%) andhighest percentage of flower to pod (93.4%).
Potensi Jenis Dipterocarpaceae di Hutan Produksi Cagar Biosfer Pulau Siberut, Sumatera Barat Endro Subandriono; M. Bismark; N. M. Heriyanto
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p64-71

Abstract

The study ofDipterocarpaceae was done in November 2007 in SiberutBiosphere Reserve. This study conducted on threes sample plotof 0.75 ha in primery forest, logged over area (LOA) <1 yearand LOA 5 years. The result showed there were three speciesof Dipterocarpaceae: Dipterocarpus elongatus Korth., Shoreajohorensis Foxw. and Hopea mangarawan Miq. The density oftree and pole in primary forest were about 24 individual/ha and13.34 individual/ha, there were 9.3 individual/ha and 4individual/ha in LOA <1 year, than this density were 8individual/ha and 5.34 individual/ha in LOA 5 years. Forsapling and seedling, these densities were about 29individual/ha and 5.417 individual/ha in primary forest, 5individual/ha and 3.750 individual/ha in LOA <1 year, 89individual/ha and 883 individual/ha in LOA 5 years.Community couple between primary forest and LOA wich hassimilarity index value more than 50%; at tree stage, it was68,83% comparing primary forest and LOA <1 year; at polestage it was 62.62% comparing primary forest and LOA 5years; at sapling stage, there were 53.29% and 66,27%comparing with LOA <1 year and LOA 5 years; at seedlingstage it was 88.66% compared to primary forest and LOA <1year. Similarity index between LOA <1 year with LOA 5years, there were 60.26% for tree stage, 90.05% for pole stageand 51.13% for seedling stage.
Keragaman Karakter Morfologis Plasma Nutfah Spesies Padi Liar (Oryza spp.) Tintin Suhartini
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p17-28

Abstract

Wild rice spesies is relativeof cultivated rice and has many differences of its characteristic.Morphological characteristic variation on crop can be used asworking assets in breeding program. Morphologicalcharacterisation was conducted in three planting seasons in2004 until 2005 at Indonesian Center for AgriculcuralBiotechnology Research and Development green houseconsisted of 88 accessions of 18 wild rice spesies whichbelonged to 4 groups of Oryza spp. (Oryza sativa, O.officinalis, O. meyeriana, and O. ridleyi). The characteristicobserved were include 12 quantitative characters and 5qualitative characters with 23 subcharacters. The wide ofquantitative morphological character variances were found inplant high, tiller number, days of flower, total number grain/panicle, awning length and stalk diameter, while the narrowcharacter variances were found in panicle length, 1.000 grainsweigth, grain shape, total internode and internode length. Thequalitative characters variance were found in grains, stalk,ligule and leaf morphology, while panicle morphology of wildrice species was dominated by open panicle type, erect panicle,secondary branching were absent and well exserted.
Aplikasi Teknik Rekayasa Genetik dalam Perbaikan Sumber Daya Genetik Tanaman untuk Ketahanan Cekaman Biotik M. Herman
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p72-84

Abstract

The main constraint encountered in theutilization of plant genetic resources (PGR) in agriculture arebiotic stresses such as insect pests, plant diseases, and plantparasitic nematodes. The application of genetic engineeringtechniques create a great opportunity for crops improvementsparticularly for insect and plant diseases resistance. Throughgenetic engineering, genetically engineered (GE) crops havebeen developed, of which having the new traits such as resistanceto insect pests, plant diseases, and herbicide tolerance.GE crops are already widely grown and marketed in manycountries. Globally, GE crops that are commercialized consistsof four categories of traits, which are insect resistance (IR),herbicide tolerance, (HT), the combined traits of IR and HT(stacked genes), and virus resistance. Initially, GE crops hadbeen commercialized globally covering 1.7 million ha in 1996,and the cropping area increased rapidly to reach about 134million ha in 2009. Indonesia is known as a country rich inPGR, that have very high value. One of environmentallyfriendly technologies that can be applied in the utilization ofPGR in Indonesia, is genetic engineering. In Indonesia,research on plant genetic engineering had started since 1997.Commodities that are being researched to develop GE plantslimited on rice, potatoes and tomatoes. GE rice resistant tostem borer (Scirpophaga incertulas), GE potato resistant tolate blight (Phytophthora infestans), and GE tomato resistantto tomato yellow leaf curl virus (TYLCV) and cucumbermosaic virus (CMV) have been successfully developed byResearch Center for Biotechnology of Indonesian Institute ofScience and Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development(ICABIOGRAD). Those GE crops have been tested for theirresistance at the screenhouses, green houses of the biosafetycontainment, and confined field trial.

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