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INDONESIA
Indonesian Journal of Cancer Chemoprevention
Published by Indonesian Society for Cancer Chemoprevention
ISSN : 23558989     EISSN : 20880197     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, and Anti-Carcinogenesis; Cancer Detection; Stem Cell Biology; Immunology; in vitro and in silico Exploration of Chemopreventive Mechanism; and Natural Products.
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Articles 20 Documents
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Search results for , issue "Vol 2, No 3 (2011)" : 20 Documents clear
Combination of Leunca Herb Ethanolic Extract and Doxorubicin Suppresses HeLa Cells’ Growth Sarmoko Sarmoko; Dyaningtyas Dewi Pamungkas Putri; Endah Puspitasari; Anindyajati Anindyajati; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp281-285

Abstract

Leunca (Solanum nigrum L.)ethanolic extractshowedcytotoxic activity on several cancer cell lines (HepG2, HT-29) and showed anti-proliferative activityon MCF-7 cells. Its application as a combinationagent in chemotherapy will increase the effectivity and reduce the toxicity of chemotherapy. We predict that application of combinatorial chemotherapy in cancer treatment will be more effective and less toxic compared to single treatment. Our research aims to investigate the cytotoxic activitiy of leunca herbs ethanolic extract alone and in combination with doxorubicin on HeLa cell line. MTT assay was conducted to measure the growth inhibitory effect of leunca herbs ethanolic extract and combinatorial treatments. Leunca herb ethanolic extract (5, 50, 250 μg/ml) increased the cytotoxic effect of  doxorubicin compared to doxorubicin alone. The strongest cytotoxic activity resulted from the combination of 250 μg/ml leunca herbs ethanolic extract and 250 nM doxorubicin. Based on our results, leunca herbs ethanolic extract is a potential chemopreventive agent, while its molecular mechanism needs to be explored.Keywords : Leunca herbs ethanolic extract, doxorubicin, HeLa, MTT assay
MCF-7 Resistant Doxorubicin are Characterized by Lamelapodia, Strong Adhesion on Substrate and P-gp Overexpression Dyaningtyas Dewi Pamungkas Putri; Sarmoko Sarmoko; Rifki Febriansah; Endah Puspitasari; Nur Ismiyati; Aditya Fitriasari
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp304-309

Abstract

The prognosis of breast cancer patients is closely associated with the response of tumor cells to chemotherapy agent. Doxorubicin is one of the primary chemotherapeutic agents used for the treatment of breast cancer. Resistance to chemotherapy is believed to cause treatment failure in cancer patients. Furthermore, long time exposure to chemotherapeutic agent induces cancer cells resistance. MCF-7 sensitive cells used as chemoresistance model have overexpression P-gp (P-glycoprotein). Chemoresistance was established by treating MCF-7 cells with 0.5 µg/ml doxorubicin-contained medium for a week. 50% inhibiting concentration (IC50) doxorubicin on MCF-7 cells/DOX were determined using MTT assay. Western blot assay and immunocytochemistry assay was performed to determine the expression of P-gp. Morphological of MCF-7 cell/DOX was changing to become larger and have lamellapodia. IC50 value of doxorubicin was 700 nM on MCF-7/DOX and 400 nM on sensitive MCF-7 cells. The MCF-7/DOX sensitivity to doxorubicin was decreased, shown by 1.5 fold higher IC50 of doxorubicin on MCF-7/DOX compared to MCF-7 sensitive cells. Treatment doxorubicin to sensitive MCF-7 cells leads to the increasing P-gp expression. The P-gp level expression has strong correlation with the low sensitivity of MCF-7/DOX to doxorubicin.Keywords: doxorubicin, resistance cells, sensitive MCF-7 cell
In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Tati Herlina; Unang Supratman; Anas Subarnas; Supriyatna Sutardjo; Suseno Amien; Hideo Hayashi
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp286-290

Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of  1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents. Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
Anticancer Evaluation of Plants from Indonesian Tropical Rain Forests Jamilah Abbas
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp310-318

Abstract

The anticancer activities of medicinal plants from Indonesia’s tropical rainforests were investigated against Hela cell line. Maytenfoliol from leaves of Calophyllumtetrapterum 3-epibetulinic acid from stem bark of C. tomentosum Wight and D.A-friedo-oleanan-3-on from stem bark of C. moonii showed anticancer activity. The compound 3-epibetulinic acid showed the more potent anticancer activity than maytenfoliol and D.A-friedo-oleanan-3- with thean IC50 value were 3.17 μg/mL, 4.89 μg/mL and 5.63 μg/mL, respectively.Keywords: Anticancer evaluation, Hela cell line, Calophyllumtetrapterum. C. tomentosum Wight and C. moonii
The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells Astrid Ayu Maruti; Ilham Augusta F.; Dyaningtyas Dewi Pamungkas Putri; Adam Hermawan; Muthi' Ikawati
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp291-294

Abstract

Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum
Molecular Docking of Robustaflavone Derivatives on Pi3k Protein Target and The Correlation With Its Cytotoxic Activity on Cancer Cells Sri Handayani; Zalinar Udin
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp319-324

Abstract

Robustaflavone derivatives have cytotoxic effect on cancer cell. Inhibition of PI3K on cancer cell has correlation with apoptotic induction. The aim of this research is to observe interaction from robustaflavone derivatives on PI3K receptor that may give contribution to the cytotoxic effect of the compounds on cancer cell line. Geometric optimization of robustaflavone derivatives structured was done used Hyperchem 7.5 software. Arguslab 4.01 software with GAdock method applied for the docking step. The docking result showed that robustaflavone 7,4',7''- trimethyl ether (RTE) has affinity on PI3K target receptor which gave the lowest Gibbs free energy (∆G -10,855 kcal/mol).Keywords: robustaflavone, PI3K, cancer, docking
In Vitro Antioxidant Activity of Zingiber officinale, Piper retrofractum, and Their Combinations Hendri Wasito; Heny Ekowati; Fitri Fauziah hayati
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp295-298

Abstract

Many plants are used empirically as antioxidant. Plants that are frequently used in Indonesian communities are Zingiber officinale and Piper retrofractum. The aim of this research was to investigate the in vitro antioxidant activity of single ethanolic extract and the combinations ofZ. officinale and P. retrofratum using free radical scavenging DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Z. officinale and P. retrofratum were extracted by maceration using 95% ethanol for 3 x 24 hours. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The concentration of the extract (μg/mL) that was required to scavenge 50% of free radicals (IC50)was calculatedusing the percent scavenging activities of six different extract concentrations. The results showed that the single ethanolic extract of Z. Officinalle produced the highest antioxidant activity with IC50of 56 μg/mL, while the antioxidant  activity of the single ethanolic extract of  P. retrofractum produced an IC50 of 3.445 μg/mL. The IC50 of combination of Z. officinale and P. retrofratum ethanolic extracts at concentration ratios of 1: 2, 1 : 1, and 2 : 1 were 148 μg/mL, 85 μg/mL, and 73 μg/mL.Keywords: Zingiber officinale, Piper retrfratum, Antioxidant, DPPH
Naringenin Enhances the Anti-Tumor Effect of Doxorubicin on HeLa Cervical Cancer Cells Through Cytotoxic Activity and Apoptosis Induction Larasati Larasati; Indri Kusharyanti; Adam Hermawan; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp325-333

Abstract

Naringenin, an abundant flavanon in the peel of citrus fruits is reported to possess anti-proliferative effect in many cancer cells. Herein, we investigated the cytotoxic effect and apoptosis induction of naringenin in combination with doxorubicin on HeLa cells. The cytotoxicity assay of naringenin, doxorubicin, and their combination were carried out by using MTT assay. Cell viability was used as the parameters to evaluate combination effectiveness. Cell cycle distribution was determined by flow cytometry and analyzed using ModFit LT 3.0 program. Apoptosic assay was done by double staining method using Ethidium Bromide-Acridine Orange. Investigation on the expression of Bax and Bcl-2 were determined by immunocytochemistry method. Naringenin and doxorubicin showed cytotoxic effect on HeLa cells with their IC50 values of 195 µM and 1 µM, respectively. Whereas combination of naringenin - doxorubicin showed greater cytotoxicity compared the single treatment of doxorubicin. The strongest cytotoxic activity was observed at a combination of 100 µM naringenin and 0,5 µM doxorubicin. Single treatment of 0,5 µM doxorubicin for 24 hours on HeLa cells induced S-phase arrest while 100 µM naringenin did not affect on HeLa cell cycle. The combination induced S-phase arrest with the increased of sub-G1 phase percentage. In accordance with the flow cytometry results, the double staining apoptosis assay results showed the increase of apoptotic cells. Naringenin, doxorubicin, and their combination also increased the expression of Bax and decreased the expression of Bcl-2. These results concluded that naringenin was a potential co-chemotherapy agent for cervical cancer due to its synergism with doxorubicin.Keywords: co-chemotherapy, naringenin, doxorubicin, HeLa cells, cytotoxicity, cell cycle, apoptosis
Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer Nunuk Aries Nurulita; Edy Meiyanto; Sugiyanto Sugiyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp274-280

Abstract

Gynura procumbens is widely used as traditional remedy in South-East Asia. Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric and HPLC. MTT (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which contained small amount of quercetin as identified from chromatographic profile both HPTLC and HPLC. FEG inhibited cell growth of WiDr, of MCF-7 and of T47D cells in time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main active compound of FEG. At this study, FEG caused less inhibition on the growth of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective effect against cancer cells and reveals prospective properties as cancer chemoprevention agent.Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivity
Antiproliferative Effect and Apoptosis Induced in Human Cell Lines by Bruguiera gymnorhiza Barks Methanol Extract Warsinah Warsinah; Sismindari Sismindari; Ratna Asmah Susidarti
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp299-303

Abstract

The Antiproliferative effects of methanol extract from Bruguiera gymnorhiza (B. gymnorhiza) barks were tested in vitro against three human cell lines: Hela, Raji and Myeloma cells. The extract was found to have antiproliferative effects against Hela, Raji and Myeloma cells with an IC50 value of 133, 504 and 384 µg/mL, respectively. The antiproliferative test was then performed on Hela, Raji, and Myeloma cells. Cytotoxicity assay of the extract was then determined using MTT method. There were some indications of apoptosis, such DNA fragmentation, as assessed by acrydine orange- ethidium bromide staining. These results indicate that extract from B. gymnorhiza barks can induce apoptosis in human cell lines.Keywords: Antiproliferative, Apoptosis, B. gymnorhiza

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