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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Vol 44, No 3 (2019): September" : 12 Documents clear
Hydrolyzation of duck meat protein using Bacillus cereus TD5B protease, pepsin, trypsin and their potency as an angiotensin converting enzyme inhibitor Winarti, A.; Rahmawati, F.; Fitriyanto, N. A.; Jamhari, J.; Erwanto, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.266-276

Abstract

This study was aimed to explore their potency of protein-bioactive of native ducks-meat after enzymatic hydrolysis by Bacillus-cereus TD5B-protease, Pepsin, and Trypsin as an angiotensin-converting enzyme (ACE) inhibitor. The samples: ducks-meats from 10 months age of male Mojosari and Magelang-Duck. The experiments: individually hydrolysis of meat-protein using protease-enzyme (0.1 % w/w) from Bacillus-cereus TD5B, Pepsin, or Trypsin. The observed parameters: protein concentration, protein molecular weight, ACE-inhibitor activity, and IC-value (IC50). Data of protein concentration were statistically analyzed using T-Test, while data of SDS-PAGE and ACE-inhibiting activity were analyzed descriptively. The results showed that soluble protein concentration increased due to the hydrolysis process, from 0.826±0.108 mg/mL to 1.050±0.197 mg/mL (Microbial-protease), 2.122±0.141 mg/mL (pepsin), 1.641±0.071 mg/mL (trypsin) for Mojosari-duck and 0.642±0.038 mg/mL to 1.171±0.534 mg/mL(Microbial-protease), 2.100±0.376 mg/mL(pepsin), 1.725±0.092 mg/mL(trypsin) for Magelang-duck. The SDS-PAGE pattern showed that there was a decrease of molecular weight of duck-meats due to the hydrolysis process, from the range of 196.53-43.88 kDa to the range of 71.35-10.12 kDa. Duck-meat protein hydrolysate had ACE-inhibiting activity 71.7%(Mojosari-Microbial-Protease) IC50 54μg/mL, 57%(Mojosari-Pepsin) IC50 151μg/mL, 75.8%(Mojosari-Trypsin) IC50 51μg/mL and 52.8%(Magelang-Microbial-Protease) IC50 83μg/mL, 78,5%(Magelang-Pepsin) IC50 85μg/mL, 83.9%(Magelang-Trypsin) IC50 22μg/mL. In conclusion, hydrolysate of Magelang duck-meat used Trypsin had better potency as an ACE-inhibitor. 
Analysis in making decision of farmer to select bull frozen semen in Indonesia Agustine, R.; Bintara, S.; Andarwati, S.; Muzayyanah, M. A. U.; Widi, T. S. M.; Putra, A. R. S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.323-332

Abstract

The research objectives was to analyze the farmer’s decision in selecting breed of bull frozen semen for Artificial Insemination (AI) and determine the factors influencing the decision. There were five bull frozen semen options which simulated in this research, such as Simmental, Limousine, Ongole Grade (Peranakan Ongole / PO), Brahman, and other option. ”Other” option was used if the farmer did not recognize what breed of frozen semen was inseminated to their cow. Farmers must choose only one option of bull frozen semen that suitable for their cow. This study was conducted by collecting data using questionaire from 400 beef cattle’s farmers in Central Java Province and Yogyakarta Province. Respondents were selected by multistage random sampling technique. Descriptive statistical analysis and multinomial logistic regression analysis was used in this study The results revealed that 42% of farmers were more interested in inseminating their cows with bull semen from Simmental, and 30.75% of farmers were more interested in Limousin. Other variables such as family member, AI’s cost, land ownership, cow breed which lastly being inseminated, geographical factor, and farmer’s knowledge about the inseminatorswere variables that determine farmer’s decision process.

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