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Articles 16 Documents

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Search results for , issue "Vol 2, No 2 (2013)" : 16 Documents clear

Analysis of Radiant Gamma Illumination Effects on Pancreatic Insulin Levels Before and After The Administration of Pare Fruit Extract (M. charantia) On Weed Mice Glucose Zulkarnain Zulkarnain; Johan A. E. Noor; Unggul P Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Gamma rays are ionizing radiation causes cells damage. Cells damage was caused by radiation related to free radicals as a product when the interaction was happened. The cell defense mechanisms against free radicals involves an active compound called antioxidant that can neutralize it. The objectives of this research were to analyze effects of gamma radiation and to investigate the effects of M. charantia extract giving on the pancreas resistance in producing insulin. One hundred eighty mice were used in this research. They were divided into four group: negatif control (K-), radiation non-extract (R-), positif control (K+) and radiation with extract (R+). The Mice were treated with 200 mg/kg BB, 400 mg/kg BB, 600 mg/kg BB, 800 mg/kg BB, and 1000 mg/kg BB. Exposure of gamma rays is given fractionally for five days with 100 rad per fraction ranging from 100 rad, 200 rad, 300 rad, 400 rad and 500 rad per treatment. Insulin levels was measured by ELISA. The results showed that radiation exposure reduced the insulin levels, increase in radiation dose causes increasing of reduction of insulin levels. The giving of M. charantia extract reduced of reduction of insulin levels and also maintained the insulin levels.

Radiation Effects on Decreased Estrogen with Isoflavone Consumption to Prevent Early Menopause in Radiation Therapy Yeni Cahyati; Didik Rahadi Santoso; Unggul P Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Radiation effect is one of which is the reduction in estrogen. Gamma rays are ionizing radiation that can lead to cell damage due to free radicals produced when the radiation interacts with cells. The level of cell damage can be minimized by antioxidants. Antioxidants used is the isoflavones of soy milk, so it can be know the effects of radiation on the decline in estrogen that accompanied the consumption of isoflavones to prevent early menopause in radiation therapy.Radiation exposure are given fractionally. The dose was 1,5 Gy in once exposure and given every 3 days up to a maximum dose of the treatment groups. Consumption of soy milk to the mice done orally. Blood was drawn and serum was made. Serum estrogen levels will be tested by using ELISA.The results showed that the ovarium disfunction and stress experienced by mice (Mus musculus) caused by radiation exposure. Maximum results in the consumption of isoflavones on estrogen levels the subject to radiation exposure is 44 mg/day and 131 mg/day of soy milk. This isoflavone dose increase in estrogen levels maximumly. The increase was the response given by the body's immune system of mice due to start reacting and isoflavones are phytoestrogens.

Biodegradable Plastics Functional Groups Methyl Acrylate and Pati Garut Relationship Against Mechanical Properties S J Iswarin; Lailatin Nuriyah; A I Sriwilujeng
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

In order to addressing the problem of plastic waste accumulating, we made biodegradable plastic from starch with methyl acrylate as initiator. The biodegradable plastics are made by grafting method on a variety of arrowroot starch composition and methyl acrylate. Variations in the composition of arrowroot starch and methyl acrylate used was 30:70; 35:65; 40:60; 45:55; and 50:50.Results of the mechanical tests e.g. tensile strength and percent elongation on plastics, show that the best value is obtained on the composition of 30:70. It reaches of (14.8 ± 2.8) MPa in tensile strength, and (5.33 ± 0.08) % in elongation percent. This value is in accordance with the results of FTIR analysis. In here, the value of tensile strength is consistence with the value of absorbance of methyl acrylate, which indicates the concentration of functional groups C-C or the number of functional groups that formed. The greater of the concentration will be the greater of the tensile strength, while the percent elongation values are influenced by the number of functional groups C-O-C formed by arrow root starch.

Thermal Neutron Flux Measurements at Room Treatment LINAC iX 15 MV Risalatul Latifah; Johan A E Noor; Bunawas Bunawas
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Linac is operated at above 10 MV will generate secondary radiation in the form of neutron emission from the reaction of the high photon energy linac striking the material components of linac such us targets, collimators and filters. Secondary radiation will increase the probability of the secondary cancer risk in patients due to increasing the dose of radiation received. This study evaluated the thermal neutron flux at the indoor spatial operated 15 MV linac using foil activation method. A total of 14 Indium-115 foil placed in the treatment room were exposed to 15 MV linac plane for 1 minute. Results showed the highest flux are around isocenter . With the flux value of additional dose due to the neutron flux at Sv/menit. FLux decreases as distance function. Related to radiation protection efforts for radiation workers, the contribution coming from the neutron dose necessary acted upon by adding shielding on the door.

Triterpenoid Fraction Antimalarial Activity Test from Methanol Extract Made by Leaf Artocarpus camansi Against Plasmodium Berghei by In Vivo Ramadhani Sucilestari; Dwi Soelistya D J; Imam Bachtiar
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Malaria disease is still a serious health problem in Indonesia. This study aimed to determine the antimalarial activity of triterpenoid compound fraction of A. camansi leaf methanol extract to P. berghei of in vivo. This experiment of using research a completely randomize design (CRD) with four different doses (0.1 mg/kg BW, 1 mg/kg BW, 10 mg/kg BW, 100 mg/kg BW) and one negative control group (1 % CMC). The results show that triterpenoid compound fraction of A. camansi leaf methanol extract was effective to reduce parasite activity of P. berghei. The most effective dose was 100 mg/kg BW for three days with daily treatment after the mice was infected by parasite.

Utilization of Pseudomonas putida and Pseudomonas fluorescens as Biosensor to Measure BOD5 In Water Level Sri Sugiarti; Setyawan P. Sakti; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Along with population growth, the need for clean water is increasing. The availability of clean water is starting to decrease due to the decline in quality and quantity of water in the environment. Water quality degradation can be caused by water pollution from industrial waste disposal. One of the parameters to measure the level of pollution is the biochemical oxygen demand (BOD). Beside titration, biosensor method is a method developed to measure the BOD. The purpose of this study is to design a microorganism-based biosensor that can be used to measure levels of Biochemical Oxygen Demand (BOD5) using a microelectrode transducer with Pseudomonas putida and Pseudomonas fluorescents as bioreceptor on phenol wastes. BOD concentration was controlled by adding phenol in water with concentration of 0 mg/L, 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L. BOD concentration as measured by using titration method and developed biosensor. Experimental result shows that the potential of the biosensor linearly correlated to the concentration of BOD in water. Membrane surface area does not contribute any effect to the output potential of the biosensor. We can conclude that both Pseudomonas putida and Pseudomonas fluorescents can be use as material for measuring BOD concentration in water.

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