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Environmental Science

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Articles 6 Documents

Search results for , issue "Vol 4, No 2 (2017)" : 6 Documents clear

Study of Stearic Acid Layer (SA) Microstructure on Surface Quartz Crystal Microbalance (QCM) Sensors Nurramdaniyah Nurramdaniyah; Masdiana Padega; Djoko Herry Santjojo; Setyawan P. Sakti; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

In the development of QCM-based biosensor sensors, the sensor surface is a very important part because it determine how the surface interacting with its environment especially during the immobilization of biomolecules process. In the utilization of QCM as biomolecule detecting biosensor, it is necessary to coat the polymer material on the surface of QCM. A stearic acid layer is used as an immobilization matrix layer because stearic acid has both polar and non polar properties so it can interact with biomolecules. The stearic acid layer is deposited on the polystyrene surface using a vacuum evaporation method. A study of the microstructure of the stearic acid coating was conducted to determine the possibility of the deposited stearic acid layer used as the matrix layer. In this research, microstructural study of layers of the stearic acid by using Scanning Electron Microscope (SEM) was carried out to find out the surface morphology of stearic acid. X-Ray Difraction (XRD) measurements were conducted to identify the crystal structure of stearic acid while Fourier Transform Infrared (FTIR) measurements were used to determine the stearic acid functional group. The Scanning Electron Microscope (SEM) results show the surface of the cavity and pore layers of the polystyrene layer was coated with white fibers homogeneously deposited on the surface of QCM / Polystyrene. Furthermore, the X-Ray Difraction (XRD) results showing a spectrum peak at an angle of 2q = 26,66° indicates the existence of stearic acid crystals. Whereas, the measurement results of the Fourier Transform Infrared (FTIR) indicate the appearance of C-H functional groups at a wavelength value of 3600-2600 cm-1 which indicates the functionality of the stearic acid layer.

The Effect of Smoking Behavior in the Human Population Growth of Lung Cancer Patients Lu’luul Wardah; Trisilowati Trisilowati; Wuryansari Muharini Kusumawinahyu
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

This article discusses a model of lung cancer as the effect of smoking behavior on both active and passive smoker. There are four subpopulations in this model, namely susceptible subpopulation, active smoker subpopulation, passive smoker subpopulation, and subpopulation of lung cancer. Dynamical analysis is conducted to determine the equilibrium point, existence condition for equilibrium point, and analyze their stability. Based on analysis result, there are three equilibrium points. First equilibrium point shows that all subpopulations extinct. Second equilibrium point shows that only susceptible subpopulation can survive, and the last equilibrium point shows that all subpopulations can survive. First equilibrium point always exists while the others exist under certain condition. The stability of first equilibrium point can be reached when the intrinsic growth rate is less than the death rate. Whereas, the others equilibrium points will be stable under certain condition. Numerical simulation is performed to illustrate the analysis result. It is shown that numerical results are in accordance with analysis result. These numerical simulations also indicate that the rate of passive smoker plays important role in the growth rate of lung cancer.

Effect of Spirulina Extract on Diazinon Effect at the Channel Membrane Albumin and Vitelin Membranes Based on Strong Flows and Voltage Dian Rizqi Nur Amalia; Abdurrouf Abdurrouf; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

The use of pesticides in foods can be bad for human health, so the danger of pesticide use in food can damage cell metabolism or permeability of the cell membrane. This study aims to analyze the effect of Spirulina extract on the potential difference of egg cell membrane of albumin and vitellin chickens against anti channel blocker on diazinon. The contaminant used in this research is diazinon 600 EC. How to overcome the contamination of livestock products that can be harmful to health one of them using antioxidants. Thus, the administration of Spirulina extract can affect the potential response of egg cell membranes (Gallus Gallus domesticus) in the polluted food of diazinon. Potential rabbit egg membrane can be measured by using microelectrode associated with PicoScope 5000 series, then the data appears will be displayed through the PC. The results of this measurement showed that along with the increase of diazinon solution concentration, the potential value of egg cell membrane produced decreased. While the influence of Spirulina extracts in the egg (Gallus gallus domesticus) membrane that has been contaminated diazinon showed the potential value of membrane bigger/higher. This is because in the extract Spirulina has phenolic and flavonoid content that has the potential as an antioxidant that can be useful to reduce the level of free radical reactivity caused by diazinon.

Analysis of Voltage Difference Characteristics of Albumin Membranes and Vitelin Membranes of Chicken Eggs through Diazinon Effects Wira Dian Jauharah; Didik Rahadi Santoso; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

Cells are the most important structural and functional part of any organism. Cells are protected by cell membranes that envelop cells, and separate cells with their environment. The effectiveness of the work of a cell membrane can be disrupted for various reasons, one of which is pesticide contamination. Pesticides in cells can damage cell metabolism or cell membrane permeability. Therefore, this study aims to determine how big the effect of diazinon insecticides on the characteristics of different potential membrane vitelin and membrane albumin chicken egg cells. Measurements were made using the microelectrode connected with the picoscope 5000 series, then the data appears displayed through the PC. The results of membrane potential measurements showed that the greater the concentration of insecticide given, the potential value of chicken egg membrane increased. Potential Differential Values Membrane of eggs with different concentrations ranged in -145 mV - 249 mV on vitelin membrane, and -142,5 mV - 242,5 mV on albumin membrane

The effectiveness study of SnCl2.2H2O masking on the formation of QCM profile using wet etching technique with KOH solution Tri Andi Rusly; D.J. Djoko H. Santjojo; Setyawan P. Sakti; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

SnCl2.2H2O has been successfully utilized to produce isotropic profile on the surface of a SiO2 crystall by a wet etching procces. The etching process was carried out by using a 40% KOH solution. In this research, the wet etching method is carried out by optimizing the etch time which was varied for 0,5 hour, 1 hour, 1,5 hour. The profile was determined by applying a SnCl2.2H2O on the surface of a QCM. The mask was applied by using a spray coating technique. The SnCl2.2H2O material was chosen as the masking material because it has a high adhesive property to SiO2, does not react to the KOH etching solution and is corrosion resistance as well as low cost. The etching profile surface results and etch depth (Dz) were determined using topography measurement system (TMS 1200). The etching rate with 40% KOH during etching time of 30 minutes, 60 minutes, 90 minutes were 4.36 μm, 4.56 μm and 5.79 μm respectively. The optimum QCM surface isotropic profile was obtained at the etching rate of 4.56 μm / h by 60 minutes etching.

Optimization of Pesticide Analysis Method for Diazinon and Chlorantraniliprol Using High Performance Liquid Chromatography (HPLC) Marselina Theresia Djue Tea; Akhmad Sabarudin; Hermin Sulistyarti
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

Methods of obtaining diazinone and chlorantraniliprol in soil samples have been developed. Analytes were extracted using acetonitrile by using an ultrasonic solvent extraction method (USE). The discovery uses high performance liquid chromatography using a UV detector. The optimum conditions for the separation of both pesticides are Acetonitrile composition: Air (60:40), flow rate 0.4 mL / min, and at 220 nm wavelength. The more the amount of acetonitrile in the eluent mixture the faster the retention time in the column. Retention time respectively, chlorantraniliprol 10,525, and diazinon 21,029.

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