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Indonesian Journal of Tropical and Infectious Disease

IJTID
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Published by Universitas Airlangga

ISSN : 20851103 EISSN : 23560991 DOI : -

Core Subject : Health, Science,

Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

This journal is a peer-reviewed journal established to promote the recognition of emerging and reemerging diseases specifically in Indonesia, South East Asia, other tropical countries and around the world, and to improve the understanding of factors involved in disease emergence, prevention, and elimination. The journal is intended for scientists, clinicians, and professionals in infectious diseases and related sciences. We welcome contributions from infectious disease specialists in academia, industry, clinical practice, public health, and pharmacy, as well as from specialists in economics, social sciences and other disciplines.

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Articles 6 Documents

Search results for , issue "Vol. 7 No. 6 (2019)" : 6 Documents clear

IDENTIFICATION AND PREVALENCE OF GASTROINTESTINAL PARASITES IN BEEF CATTLE IN SIAK SRI INDRAPURA, RIAU, INDONESIA Suwanti, Lucia Tri
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Gastrointestinal (GI) parasites infection are one of the major constraints cattle farm in tropical countries including Indonesia and some of GI parasites in cattle have the potential to transmit to humans. This study was aimed to identify and determine the level of prevalence of gastrointestinal (GI) parasites in beef cattle in Siak Sri Indrapura District, Riau Province, Indonesia. This research was conducted on 100 beef cattle consisted of, respectively, 32, 34 and 34 cattles from Bungaraya, Sabak Auh, and Dayun sub-district. The characteristic of sample such as age and sex cattle, cage management, feed and drinking water were recorded. Native, sedimentation and sucrose flotation methods were used to find protozoa and eggs worm. We assessed GI parasites based on finding eggs worm, protozoan cyst and coccidial oocysts in stool samples and identification of GI parasite was based on the morphology and size of the eggs worm and cysts or oocysts of protozoan. The result showed that all of 100 feces samples that examined 100% positive infection for parasites. There were eleven types of gastrointestinal parasites that have been identified, 6 genera of protozoan and 5 genera of worms. The prevalence of gastrointestinal parasites in beef cattles in Siak Sri Indrapura District were Blastocystis sp. (100%), Entamoeba sp. (90%), Eimeria sp. (53%), Giardia sp. (7%), Balantidium coli (4%), Cryptosporidium sp. (2%) Oesophagustomum sp. (45%), Toxocara vitulorum (20%), Moniezia expansa (9%), Trichuris sp. (5%), and Fasciola sp. (4%). In conclusion, Siak Sri Indrapura Riau is an endemic GI parasite and this can threaten the health of livestock and potentially as a zoonotic transmission.

MTT FORMAZAN REPLACED WST-8 AS A BETTER SIMPLE SCREENING METHOD FOR DETECTION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY Tantular, Indah
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

We have previously developed the WST-8 method as a simple and rapid screening test for detection of glucose-6-phosphate dehydrogenase (G6PD) deficiency accomplished by the naked eye. However, it was little difficult to distinguish between faint orange colors developed by heterozygous females and pink colors of normal hemolyzed blood, since both have similar tones. To solve this problem, we established a new and simple screening method that utilizes another formazan substrate, MTT (3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2H tetrazolium bromide) in combination with a hydrogen carrier, 1-methoxy phenazine methosulfate. MTT formazan exhibits a purple color, thus allowing for the ability to easily distinguish the pink colors of hemolyzed blood. However, MTT has been reported to react with hemoglobin non-specifically and to interfere with the interpretation of the color reaction. In our examinations by mixing MTT with hemolyzed blood, we found that the non-specific reaction was very slow, and that the addition of a small amount of blood (5~10 Î¼l) into a reaction mixture (800 Î¼l) did not interfere with the reaction of G6PD activity. In this new MTT method, a strong purple color was generated in normal blood samples at 20~30 min after incubation, which could be distinguished by the naked eye from G6PD-deficient blood samples with less than 50% residual activity. In addition, quantitative measurement using a spectrophotometer was also possible despite the fact that MTT formazan is water-insoluble.

DETECTION OF HELICOBACTER PYLORI INFECTION IN CHRONIC GASTRITIS BIOPSY SPECIMEN USING WARTHIN-STARRY AND MODIFIED GIEMSA STAIN IN DR SOETOMO HOSPITAL SURABAYA Sandhika, Willy
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Helicobacter pylori is a bacteria that commonly cause chronic gastritis. Identification of its infection is essential for eradication treatment. Detection of H.pylori bacteria in gastric biopsy specimen by histology method is a diagnostic tool that widely accepted because it is superior to serology examination. Although the bacteria can be seen in routinely Hematoxylin-Eosin staining, Modified Giemsa and Whartin Starry stain was commonly used to see the bacteria more clearly. Whartin Starry stain gives more contrast to the bacteria but modified Giemsa stain is preferable at many centres because it is a cheaper and simpler method. This study want to find out whether there is difference result in detection of H.pylori using these two stains. Material and methods. Paraffin blocks from gastric biopsy patients with chronic gastritis were retrieved from Anatomic Pathology Laboratory Dr.Soetomo Hospital Surabaya in the year 2017. Thirty paraffin blocks were taken randomly and were made into microscopic slides for staining with Warthin-starry and modified Giemsa stain concomitantly. Results. Specimen with Whartin starry stain show 19 out of 30 were positive for H.pylori while modified-Giemsa stain found 16 out of 30 specimen were positive for H.pylori. Whartin Starry stain use silver reagent to coat the bacteria so it become more clearly visible. Conclusion. Detection of H.pylori Warthin-starry stain give more chance to obtain positive result because it use silver technique that coat the bacteria making it is more clearly visible in microscopic examination.

INCIDENCE OF DENGUE HEMORRHAGIC FEVER (DHF) IN SEMARANG COASTAL AREA: EPIDEMIOLOGY DESCRIPTIVE CASE AND BIONOMIC VECTOR Martini, Martini
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

North Semarang sub-district is located on the coast of the Java Sea. The coastal area is characterized by high salt content on both the ground and the water compare to other areas. The high salt content environment should have limited the breeding of Dengue Hemorrhagic Fever (DHF) vectors; yet, quite high incidents of DHF cases are reported taken place in North Semarang coastal area. The aim of this study was to describe the epidemiology of DHF incidence, characteristic of cases, and bionomics vector in the coastal area of North Semarang sub-district. This study was applied descriptive observational design to analyze samples consisting of 62 dengue cases and 184 houses. The research variables consisted of coordinate of DHF cases, water salinity, House Index (HI), Container Index (CI), and Aedes species. Data were processed using SPSS in a bivariate manner; while, mapping was analyzed spatially using ArcGIS 10.3. A total of 184 houses were surveyed and 55 cases of DHF were identified. Most cases occurred in 6 -16 year age group (47.3%), water salinity ranged from 2-3%, indicating that the water in the coastal area tended to be brackish water. The results of the Pearson Correlation test showed that there was no relationship between HI and Incidence Rate (IR) of DHF in North Semarang District. Aedes aegypti was identified in a positive container, otherwise Aedes albopictus was not found. DHF cases mostly occurred in school age groups, and were distributed in all villages near or far from the beach. DHF vector could breed in areas with little brackish water, so that dengue transmission might occur in this area.

PREVALENCE, INTENSITY AND RISK FACTORS OF SOIL TRANSMITTED HELMINTHS INFECTIONS AMONG ELEMENTARY SCHOOL STUDENTS IN NGIS VILLAGE, KARANGASEM DISTRICT, BALI Arta Suryantari, Sang Ayu
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Soil Transmitted Helminths (STH) infections is one of health issues in Indonesia that has environment and social basis. It is classified as neglected disease. The Indonesian government already has eradication program, but it is not supported by evaluation and monitoring program. The purpose of this study is to determine the prevalence and relation of each risk factors related to STH infections in elementary school in Ngis village, Karangasem regency, Bali. The study was done by analytical description using cross sectional study. Samples were selected from population based on inclusion and exclusion criteria. Primary data about suspected risk factors were collected using questionnaire. Diagnosis was established using Kato-Katz modification method. Data was analyzed using chi-square with confidence interval 95% or p value â‰¤0.05 categorized as significant. 138 students enrolled in this study, the median age is 9 (6-13) years. The prevalence of STH infections is 10.1% with 78.6% is single infection of Trichuris trichiura and 21.4% mixed infections. The proportion of STH infections in males is higher than female but it is statistically insignificant. STH infections have significant relationship with some risk factors such as not washing hand after defecation, not washing hand after playing with soil, barefoot, not cutting nails and consuming anti-helminthic routinely. The highest risk factor of STH infections in Ngis village is not having available and proper latrine. (OR=33.9; 95%CI=5.749-199.769). The prevalence of STH infection is quite high with mild to moderate intensity and risk factors namely low hygiene and limited latrines. The implementation of monitoring and evaluation can be an effort to control risk factors and stop the STH transmission chain.

DIVERSITY, DOMINANCY, AND PERIODICITY OF MOSQUITOES IN FILARIASIS ENDEMIC AREAS IN SAMBOREJO VILLAGE TIRTO DISTRICT PEKALONGAN REGENCY Wibawaning Astuti, Raden Roro Upiek Ngesti
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 6 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Vector-borne mosquito diseases are still as a public health problem in the world, including in Indonesia. Many of mosquitoes species are significantly as vectors of patogen, such as virus, bacteria, protozoan, and helminths due to human health. Samborejo Village is one of filariasis endemic areas and it is still in a high mf-rate. Each of mosquito species has a differential of distribution, bioactivities pattern, and type of habitat of their breeding sites with others. The objective of this study was to determine the diversity, dominancy, and periodicity pattern of mosquitoes during night time in Samborejo Village Tirto Distric Pekalongan Regency. Mosquitoes collections were done by landing method, from 6 pm - 6 am of in an hour period of collection, for biting and resting activities and also for indoor and outdoor collection respectively. Mosquitoes were then identified and the diversity was analized by Shannon-Wienner Index. The total number of each species was served in percent. Totally there were 339 collected mosquitoes, consisting of 165 (48.67%) females and 174 (51.33%) males. Of all, there were 4 species identified which were Culex quinquefasciatus (92.1%), Culex tritaeniorhynchus (0.6%), Culex vishnui (1.8%), and Aedes aegypti (5.5%). Samborejo Village showed in low diversity with the index of 0.338, and Cx. quinquefasciatus to be the dominant species in this area. Culex quinquefasciatus also became the frequent species in each period of collection for indoor and outdoor, and it showed the indoor active biting at 9 pm, 01 am, and 03 am; furthermore, the outdoor active biting was at midnight (00) and at 03 am. However, Aedes mosquitoe was showed active biting in earlier, it was at 6 pm, 7 pm, and at 02 am.

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