cover
Contact Name
Fiametta Ayu Purwandari
Contact Email
fiametta@ugm.ac.id
Phone
+6285712601130
Journal Mail Official
agritech@ugm.ac.id
Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
agriTECH
ISSN : 02160455     EISSN : 25273825     DOI : 10.22146/agritech
Core Subject : Agriculture,
Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).
Articles 1,392 Documents
Formulasi Bubuk Minuman Berbahan Dasar Beras Hitam (Oryza sativa L. indica) untuk Lansia Penyandang Diabetes Mellitus Tipe 2 Rianita Pramitasari; Mary Astuti; Yustinus Marsono
agriTECH Vol 38, No 1 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.082 KB) | DOI: 10.22146/agritech.12161

Abstract

The aim of this research was to get a formula of black rice powder beverage for elderly with type 2 diabetes mellitus. The formulation was done by mixing gelatinized black rice powder with black soybean protein extract, black rice anthocyanin extract, aspartame, and salt based on total energy calculation, taste, and anthocyanin content. The analysis included chemical composition (moisture, ash, total protein, total lipid, and carbohydrate content) of gelatinized black rice, black soybean protein extract, and total anthocyanin content. Nutritional composition, sensory, antioxidant, and recommended dietary allowance of beverage powder was examined. The result showed that each serving with weight 19.91 g/200 mL contained 100 mg of anthocyanin, 10.34 g of carbohydrate, 5.91 g of protein, and 0.77 g of fat. The beverage had 4.8 (neutral-rather like) of preference level based on sensory analysis in elderly.  The antioxidant activity based on DPPH radical scavenging ability was 93.33±0.95 % of and could suffice 3.6–4.52% of total energy/serving based on recommended dietary allowance. ABSTRAKPenelitian ini bertujuan untuk mendapatkan formula minuman bubuk berbahan dasar beras hitam untuk lansia penyandang diabetes mellitus (DM) tipe 2. Formulasi minuman bubuk dilakukan dengan mencampur bubuk nasi beras hitam dengan ekstrak protein kedelai hitam, ekstrak antosianin beras hitam, aspartam, dan garam dapur berdasarkan perhitungan energi total, cita rasa, dan kadar antosianin total. Analisis meliputi komposisi kimia (kadar air, abu, protein total, lemak total, dan karbohidrat) bubuk nasi beras hitam, ekstrak protein kedelai hitam dan kadar antosianin total. Pada minuman bubuk hasil formulasi dihitung komposisi nilai gizi, uji kesukaan, aktivitas antioksidan, dan Angka Kecukupan Gizi (AKG). Hasil penelitian menunjukkan bahwa satu takaran saji dengan berat 19,91 g/200 mL mengandung antosianin 100 mg/takaran saji, karbohidrat 10,34 g, protein 5,91 g, dan lemak 0,77 g. Minuman yang dihasilkan memiliki tingkat kesukaan sebesar 4,8 (netral-agak menyukai) berdasarkan uji sensoris pada lansia, memiliki aktivitas antioksidan 93,33±0,95% berdasarkan kemampuan penangkapan radikal DPPH, dan mampu mencukupi 3,68–4,52% kebutuhan energi total/takaran saji berdasarkan AKG lansia per hari.
Penerapan Fuzzy TOPSIS dalam Penentuan Lokasi Kawasan Pengembangan Rantai Pasok Bioenergi Kelapa Sawit Petir Papilo; Taufik Djatna; Yandra Arkeman; Marimin Marimin
agriTECH Vol 38, No 1 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.361 KB) | DOI: 10.22146/agritech.12528

Abstract

This study aims to obtain the best location of the target area of agro-industrial development of bioenergy in the supply chain. Through a systems approach, using fuzzy TOPSIS, an analysis of various key criteria was conducted, including availability of raw materials, land conditions and prices, the ease of access to transportation, the distance to the city center, the availability of labor at the target location, availability of infrastructure – such as water and electricity, the use of the location and orientation of multiplier effect. Based on the results of the analysis that has been done, from the five areas being targeted for locations of bioenergy agro-industry development in the province of Riau, including Industrial Area Kuala Enok; Palm Oil Region in Rokan Hulu; Pelintung Dumai Industrial Area; Palm Oil Region in Bagan Batu Rokan Hilir and Teknopolitan Region in Pelalawan, the one that has been voted the best location is Pelintung Dumai Industrial Area. ABSTRAKPenelitian ini bertujuan untuk memperoleh lokasi terbaik yang menjadi target kawasan pengembangan agroindustri bioenergi dalam satu mata rantai pasokan. Melalui pendekatan sistem, dengan menggunakan metode fuzzy TOPSIS, dilakukan analisis terhadap berbagai kriteria penting diantaranya ketersediaan bahan baku, kondisi lahan dan harga, kemudahan akses transportasi, jarak lokasi dengan pusat kota, ketersediaan tenaga kerja pada lokasi sasaran, ketersediaan infrastruktur seperti air dan listrik, orientasi pemanfaatan lokasi serta multiflier effect.  Berdasarakan hasil analisis yang telah dilakukan, dari lima target kawasan yang menjadi sasaran lokasi pengembangan agroindustri bioenergi di Provinsi Riau, diantaranya Kawasan Industri Kuala Enok, Kawasan Perkebunan Kelapa Sawit di Rokan Hulu, Kawasan Industri Pelintung Dumai, Kawasan Perkebunan Kelapa Sawit di Bagan Batu Rokan Hilir dan Kawasan Teknopolitan di Kabupaten Pelalawan, telah terpilih lokasi terbaik, yakni di Kawasan Industri Pelintung Dumai
Diskriminasi Kopi Lanang Menggunakan UV-Visible Spectroscopy dan Metode SIMCA Diding Suhandy; Meinilwita Yulia; Yuichi Ogawa; Naoshi Kondo
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.985 KB) | DOI: 10.22146/agritech.12720

Abstract

In this research, the use of ultraviolet-visible (UV-VIS) spectral information of coffee solution in the range of 190-700 nm to classify the type of coffee into lanang and regular. The spectral data of lanang and regular coffee were acquired using UV-Vis/NIR spectrometer (JASCO Corp., Tokyo, Japan). The multivariate data analysis method, SIMCA, was used to construct the classification models which worked with the individual PCA model for each class of coffee samples. SIMCA provided the classification of the samples into one or more classes. The performance of the developed SIMCA model for each class was then evaluated in terms of its sensitivity, specificity, and accuracy. The SIMCA classification method showed that it was possible to discriminate and separate the samples into two different classes (lanang and regular coffee) satisfactory value of accuracy, sensitivity and specificity. This result could open a development of a rapid and reliable method based on UV-Vis spectra for the authentication of lanang coffee in the near future.ABSTRAKPada penelitian ini kami menggunakan informasi yang terdapat dalam spektra ultraviolet-visible dari sampel larutan kopi pada panjang gelombang 190-700 nm untuk mengklasifikasi dua jenis kopi yaitu kopi lanang dan kopi biasa (kopi bukan lanang). Spektra kedua jenis kopi tersebut diambil menggunakan alat UV-Vis/NIR spektrometer (JASCO Corp., Tokyo, Jepang). Metode analisis data berpeubah banyak bernama SIMCA digunakan untuk membangun model klasifikasi jenis kopi dengan cara membangun model PCA pada setiap kelas yaitu kelas kopi lanang dan kelas kopi biasa. Model SIMCA yang dibangun kemudian digunakan untuk mengevaluasi apakah sebuah sampel termasuk ke dalam kelas tertentu atau termasuk ke dalam lebih dari satu kelas. Kualitas model klasifikasi kemudian dievaluasi menggunakan parameter accuracy, sensitivity dan specificity. Pada penelitian ini, hasil klasifikasi menggunakan model SIMCA menunjukkan bahwa proses diskriminasi kopi lanang dan kopi biasa menghasilkan nilai accuracy, sensitivity dan specificity yang sangat memuaskan. Hasil riset ini telah membuka kemungkinan pengembangan metode yang mudah dan cepat berbasis spektra UV-visible untuk proses uji keaslian kopi lanang.
Kombinasi Maltodekstrin dan Whey sebagai Bahan Penyalut padaKarakteristik MikroenkapsulOleoresinKayu Manis (Cinnamomum burmanii) Tanalyna Hasna; Baskara Katri Anandito; Lia Umi Khasanah; Rohula Utami; Godras Jati Manuhara
agriTECH Vol 38, No 3 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.02 KB) | DOI: 10.22146/agritech.12725

Abstract

The aim of this study was to investigate the influence using combination of maltodextrin and whey (1:3, 2:2 and 3:1)  as wall material due to quality characteristic of cinnamon (Cinnamon burmanii) oleoresin microencapsulan, including yield, water content, solubility in water, microstructure appeareance, cinnamaldehyde content, and residue of solvent. First of all, the oleoresin was reached from extraction using ethanol 96 % for 4 hours. Then the oleoresin emultion was dried using spray drying method. The experimental design used in this research was Completely Randomized Design (CRD) with one factor (rasio of wall material). The result showed that combination of wall material had significant effect to yield (14,98 - 22,69%), water content (6,20 - 7,04%), residue of solvent (00,50 - 0,63%), cinnamaldehyde content (26,18 - 43,90%), and microstructure appeareance, but unsignificantly affected to solubility in water (92,85 - 95,24%).
Pengembangan Rosella Ungu (Hibiscus sabdariffa) sebagai Minuman Isotonik Berpotensi Antioksidan dan Mampu Meningkatan Kebugaran Tubuh Setyaningrum Ariviani; Gusti Fauza; Cristiva Pawestri
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.37 KB) | DOI: 10.22146/agritech.12739

Abstract

Exercise induces more body fluid loss and free radicals formation. Therefore, the body requires an intake of isotonic drink that rich in antioxidant. Plasma antioxidant has an effect on the human physical fitness. Having high level of anthocyanin and vitamin C, dark red roselle (Hibiscus sabdariffa L) has antioxidant capacity. However, the application of dark red roselle extract as an isotonic drink has not yet been reported. This research was aimed to develop red dark roselle as an isotonic drink with high level of antioxidant and ability to increase human physical fitness in exercise. This study was conducted in two stages. The first stage was extraction of dark red roselle with different solvent. The extract with highest antiradical activity was used for isotonic drink formulation. In the second stage, the antioxidant capacity and its effectiveness on improving physical fitness in exercise were evaluated on the isotonic drink with the highest level of consumer preference. The result showed that extraction technique using distilled water gave an extract with the highest antiradical activity. The isotonic drink containing 50% dark red roselle extract showed the highest consumer preference level. The drink showed to have potentially antiradical and capability for increasing physical fitness in exercise with the score of PFI (physical fitness index) reached 52.3 indicating a medium level of physical fitness.             ABSTRAKOlahraga menginduksi kehilangan cairan tubuh dan pembentukan radikal bebas yang lebih banyak. Oleh karena itu, tubuh membutuhkan asupan minuman isotonik yang kaya antioksidan. Antioksidan plasma berpengaruh pada kebugaran tubuh manusia. Rosella ungu (Hibiscus sabdariffa L) memiliki kapasitas sebagai antioksidan karena memiliki kadar antosianin dan vitamin C yang tinggi. Aplikasi ekstrak rosella ungu sebagai minuman isotonik belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk mengembangkan rosella ungu sebagai minuman isotonik yang berpotensi antioksidan dan mampu meningkatkan kebugaran tubuh pasca stress fisik. Penelitian ini dilakukan dalam beberapa tahap. Ekstraksi rosella ungu menggunakan pelarut yang berbeda dilakukan pada tahap pertama. Ekstrak dengan aktivitas antiradikal tertinggi digunakan untuk formulasi minuman isotonik. Pada tahap terakhir, minuman isotonik dengan tingkat kesukaan konsumen tertinggi dievaluasi kapasitas antioksidan dan efektivitasnya dalam peningkatan kebugaran tubuh pasca stress fisik. Hasil penelitian menunjukkan bahwa teknik ekstraksi menggunakan air sebagai pelarut menghasilkan ekstrak dengan aktivitas antiradikal tertinggi, yaitu mencapai ekuivalen 93,16 ± 3,94 mmol BHT/L. Minuman isotonik dengan penggunaan 50% ekstrak rosella ungu menunjukkan tingkat kesukaan konsumen tertinggi. Minuman isotonik rosella ungu terbukti berpotensi sebagai antiradikal dan mampu meningkatkan kebugaran tubuh pasca stress fisik dengan skor IKJ (indeks kesegaran jasmani) mencapai 52,3 yang mengindikasikan tingkat kebugaran sedang.
Desain dan Uji Kinerja Mesin Pemarut Sagu Tipe TPB 01 Ahmad Thoriq; Agus Sutejo
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5160.16 KB) | DOI: 10.22146/agritech.12789

Abstract

Sago processing conducted by the people of Papua have used grater, but it lacks of attention on food safety because one of iron part (corrosion) having contact to the sago. However, it may affect the quality of sago starch. This study aimed to design and to test the performance of machine grater use stainless steel grade eyes. The method used was through the engineering design approach consists of problem identification, formulation and refinement of the design concept, design analysis and work drawing, machinery manufacturing, functional testing and performance testing. The results showed that the designed sago grater machines has a capacity of 649.38 kg/h at a speed of 1400 rpm rotary cylinder grate, and the sago pith loss rate of 6.71%. ABSTRAKPengolahan sagu yang dilakukan kelompok masyarakat di Pulau Papua hanya menggunakan mesin pemarut. Namun mesin pemarut yang digunakan kurang memperhatikan faktor keamanan pangan karena ada bagian alat yang kontak dengan empulur sagu yang terbuat dari besi yang mudah berkarat. Hal ini tentunya akan mempengaruhi mutu dan kualitas pati sagu yang dihasilkan. Penelitian ini bertujuan untuk melakukan desain dan uji kinerja mesin pemarut sagu dengan mata parut stainless steel. Metode yang digunakan pada penelitian ini yaitu melalui pendekatan rancangan teknik yang terdiri atas indentifikasi masalah, perumusan dan penyempurnaan konsep desain, analisis desain dan gambar teknik, pembuatan mesin, uji fungsional, dan uji kinerja. Hasil penelitian menunjukkan bahwa mesin pemarut sagu hasil rancangan memiliki kapasitas 649,38 kg/jam pada kecepatan putar silinder parut 1400 rpm, dan tingkat kehilangan empulur sagu sebesar 6,71%.
Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent Fatma Zuhrotun Nisa; Mary Astuti; Sofia Mubarika Haryana; Agnes Murdiati
agriTECH Vol 39, No 1 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.981 KB) | DOI: 10.22146/agritech.12813

Abstract

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves’ extract with water extraction was lower than ethanol 70% and methanol.
Kinetika Fotodegradasi Klorofil, Tokoferol, dan Karotenoid dalam Minyak Sawit Merah Dewi Fortuna Ayu; Nuri Andarwulan; Purwiyatno Hariyadi; Eko Hari Purnomo
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.026 KB) | DOI: 10.22146/agritech.12855

Abstract

The objective of this research was to assess the photodegradation kinetics of chlorophyll, tocopherol, and carotenoid in red palm oil (RPO) during storage under flourescent light intensities of 5000, 10000, and 15000 lux. Photodegradation was followed by measuring the changes of chlorophyll, tocopherol, and carotene contents in RPO filled in transparent bottles stored in incubator box (31.60±0.69 oC) with controlled light intensity. As reference, pure RPO was filled in dark and transparent bottles then was stored at roomy temperature (31.46±1.04 °C) and lighting (476.25-484.89lux). The result showed that photodegradation of chlorophyll followed first order kinetics with two distinct photodegradation periods. First, rapid photodegradation period during the first 6 hours of storage with k values of 3.81x10-2, 4.45x10-2, 5.64x10-2 day-1, followed by a slower photodegradation period at prolonged storage more than 6 hours, with k values of 1.41x10-2, 3.01x10-2, 4.59x10-2 day-1 under light intensities of 5000, 10000, and 15000 lux, respectively. Photodegradation of tocopherol and carotene also followed first order kinetics. Photodegradation of tocopherol had the highest rate constant (k value of 9.10x10-2, 12.02x10-2, 17.33x10-2 day-1), meanwhile carotene had the lowest rate constant (k value of 0.80x10-2, 1.40x10-2, 1.98x10-2 day-1) under light intensities of 5000, 10000, and 15000 lux, respectively. The light intensity coefficient (zi) as dependence indicator of k on the changes of light intensity were measured. The zi values ofchlorophyll, tocopherol, and carotene were 20000, 33333, and 25000 lux, respectively; indicated that the degradation rate of chlorophyll was the most sensitive to the changes of light intensity. ABSTRAKPenelitian ini bertujuan untuk mengkaji laju fotodegradasi klorofil, tokoferol, dan karoten dalam minyak sawit merah (MSM) selama penyimpanan dalam kondisi terpapar cahaya fluoresen pada intensitas 5000, 10000, dan 15000 lux. Fotodegradasi dikaji dengan mengukur perubahan kadar klorofil, tokoferol, dan karoten MSM pada botol transparan yang disimpan dalam kotak inkubator (31,60±0,69 oC) dengan intensitas cahaya dipertahankan konstan. Sebagai pembanding, MSM murni dalam botol gelap dan transparan disimpan pada suhu (31,46±1,04 °C) dan pencahayaan normal laboratorium (476,25-484,89 lux). Hasil penelitian menunjukkan bahwa klorofil mengalami fotodegradasi mengikuti model reaksi ordo pertama dalam dua periode; yaitu periode fotodegradasi cepat selama penyimpanan 6 jam pertama dengan nilai konstanta laju (k) 3,81x10-2, 4,45x10-2, 5,64x10-2 hari-1, diikuti periode fotodegradasi lambat pada penyimpanan yang lebih lama dengan nilai k 1,41x10-2, 3,01x10-2, 4,59x10-2 hari-1 masing-masing pada intensitas cahaya 5000, 10000, dan 15000 lux. Fotodegradasi tokoferol dan karoten juga mengikuti model reaksi ordo pertama. Fotodegradasi tokoferol berlangsung dengan laju paling tinggi (nilai k 9,10x10-2, 12,02x10-2, 17,33x10-2 hari-1), sedangkan fotodegradasi karoten berlangsung dengan laju paling rendah (nilai k 0,80x10-2, 1,40x10-2, 1, 98x10-2 hari-1) masing-masing pada perlakuan intensitas cahaya 5000, 10000, dan 15000 lux. Konstanta intensitas cahaya (zi) sebagai indikator ketergantungan nilai k terhadap perubahan intensitas cahaya untuk klorofil, tokoferol, karoten berturut-turut adalah 20000, 33333, dan 25000 lux. Hal ini mengindikasikan bahwa laju degradasi klorofil paling sensitif terhadap perubahan intensitas cahaya.
Optimasi Proses Pembuatan Bubuk Oleoresin Lada (Piper nigrum) Melalui Proses Emulsifikasi dan Mikroenkapsulasi Firdaus Syafi'i; Christofora Hanny Wijaya; Budi Nurtama
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.64 KB) | DOI: 10.22146/agritech.12856

Abstract

The aim of this study was to improve the quality of pepper product by optimize process of emulsification and microencapsulation. The experimental design used in this study was Response Surface Methodology (RSM). The results showed that the selected emulsifier was arabic gum that had the highest solubility in water (99.78%). The optimum point of emulsification occured at 15% of concentration arabic gum and 4 minutes of homogenization time with the result was solubility in water 99.80%, and emulsion stability 97.78%. The optimum point of microencapsulation process occured at 3:1 of maltodextrin and sodium caseinate ratio, 10% of coating material concentration, and 180°C of drying temperature with the result was essential oil content 1.04%, solubility in water 98.18%, surface oil 0.20%, water content 2.45%, oil recovery 77.07%, and the yield of microcapsule 69.87%.ABSTRAKPenelitian ini bertujuan untuk memperbaiki kualitas mutu produk oleoresin lada melalui optimasi proses emulsifikasi dan mikroenkapsulasi. Rancangan percobaan yang digunakan adalah Response Surface Methodology (RSM). Hasil penelitian menunjukkan bahan pengemulsi yang terpilih adalah gum arab yang memiliki kelarutan tertinggi dalam air (99,78%). Titik optimum proses emulsifikasi terjadi pada konsentrasi gum arab 15% dan lama homogenisasi 4 menit yang memberikan nilai kelarutan dalam air 99,80% dan stabilitas emulsi 97,15%. Titik optimum proses mikroenkapsulasi terjadi pada rasio maltodekstrin dan natrium kaseinat (3:1), konsentrasi bahan penyalut 10%, dan suhu inlet pengeringan 180°C yang memberikan nilai kadar minyak atsiri 1,04%, kelarutan dalam air 98,18%, kadar surface oil 0,20%, kadar air 2,45%, oil recovery 77,07%, dan rendemen 69,87%.
Aktivitas Antioksidan Ekstrak Rumput Laut Cokelat (Sargassum polycystum) Kun Cahyaningrum; Amir Husni; Siti Ari Budhiyanti
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (826.562 KB) | DOI: 10.22146/agritech.12857

Abstract

The purpose of this study was to determine antioxidant activity of polyphenol and phlorotannin extracts from brown algae Sargassum polycystum. Polyphenol was extracted with ethanol 96% then tested total phenolic content, while phlorotannin was extracted with methanol, chloroform, aquabidest, and ethyl acetate then tested total phlorotannin content. After crude polyphenol and crude phlorotannin extracts were obtained, the studied compounds were extracted using column chromatography. The extracts were then analyzed with HPLC (High Performance Liquid Chromatograpys). Antioxidant activity was analyzed by radical scavenging activity (RSA) and ferrous ion chelating ability (FIC) for four samples, crude polyphenol, crude phlorotannin, polyphenol, and phlorotannin. The result of total phenolic content was 1.18±0.67 mg GAE/g dry sample and total phlorotannin content was 0.61±0.27 mg PGE/g dry sample. Based on RSA and FIC analysis, phlorotannin (IC50 1.12±0.02 mg/mL and 1.34±0.01 mg/mL) showed the highest activity followed by crude phlorotannin (IC50 1.20±0.01 mg/mL and 1.47±0.10 mg/mL), polyphenol (IC50 1.23±0.01 and 1.55±0.02 mg/mL), and crude polyphenol (IC50 1.27±0.01 mg/mL and 1.63±0.02 mg/mL). The results of chromatographic analysis showed that S. polycystum extract contains phenolic compounds similar to the phloroglucinol.ABSTRAKPenelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak polifenol dan florotanin dari rumput laut cokelat Sargassum polycystum. Polifenol diekstrak menggunakan pelarut etanol 96% kemudian diuji kandungan total fenolik, sedangkan florotanin diekstrak dengan menggunakan pelarut metanol, kloroform, akuades, dan etil asetat kemudian diuji kandungan total florotanin. Setelah memperoleh ekstrak polifenol dan florotanin, kedua senyawa tersebut dipurifikasi dengan kromatografi kolom. Hasil kromatografi kolom dianalisis menggunakan HPLC (High Performance Liquid Chromatography). Uji aktivitas antioksidan dilakukan dengan metode radical scavenging activity (RSA) dan ferrous ion chelating ability (FIC) terhadap empat sampel, yaitu ekstrak polifenol, ekstrak florotanin, polifenol, dan florotanin. Kandungan total fenolik yang diperoleh adalah 1,18±0,67 mg GAE/g ekstrak kering, sedangkan kandungan total florotanin diperoleh 0,61±0,27 mg PGE/g ekstrak kering. Berdasarkan uji RSA dan FIC, florotanin (IC50 1,12±0,02 mg/ mL dan 1,34±0,01 mg/mL) memiliki aktivitas tertinggi diikuti ekstrak florotanin (IC50 1,20±0,01 mg/mL dan 1,47±0,10 mg/mL), polifenol (IC50 1,23±0,01 dan 1,55±0,02 mg/mL), dan ekstrak polifenol (IC50 1,27±0,01 mg/mL dan 1,63±0,02 mg/mL). Hasil analisis kromatografi menunjukkan ekstrak S. polycystum mengandung senyawa fenolik relatif seperti senyawa floroglusinol.

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