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INDONESIA
Biopropal Industri
Published by Kementerian Perindustrian Republik Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering
Arjuna Subject : -
Articles 3 Documents
 1 
Search results for , issue "Vol 12, No 2 (2021)" : 3 Documents clear
AKTIVITAS ANTIHIPERKOLESTEROLEMIA DARI YOGHURT UMBI GARUT (Maranta arundinacea Linn) DENGAN PERBEDAAN FORMULASI KULTUR BAKTERI (Antihypercholesterolemia Activity from Arrowroot (M. arundinacea Linn) Yoghurt with Different Bacterial Culture Formulation) Sitaresmi Yuningtyas; Anggraena Roesmiena Waty; Devi Ratnasari
Biopropal Industri Vol 12, No 2 (2021)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v12i2.6937

Abstract

Hypercholesterolaemia is one of the factors causing cardiovascular disease. An alternative to prevent hypercholesterolemia is by consuming arrowroot yoghurt which has ability as hypocholesterolemic probiotic. Lactobacillus plantarum and Lactobacillus fermentum is known to have hypocholesterolemic potency. The aim of this research is to get the appropriate  formulation of the culture to produce arrowroot yoghurt which can decrease total cholesterol level. Arrowroot is fermented using different lactic acid bacteria formula, comprising A (Lactobacillus plantarum : Lactobacillus fermentum), B (Lactobacillus plantarum), and C (Lactobacillus fermentum). Arrowroot yoghurt then evaluated with total of lactic acid, pH, lactic acid bacteria, organoleptic, and in vivo total cholesterol. The results showed that the best formula of arrowroot yoghurt using C culture based on total lactic acid, pH parameter, and total lactic acid bacteria. After the total cholesterol test, the results obtained that arrowroot yoghurt which fermented by Lactobacillus fermentum and positive control (simvastatin 0,18 mg/200 g body weight) can reduce total cholesterol levels with a decrease percentage of 30.36% and 27.13%. Therefore, arrowroot yoghurt which fermented by Lactobacillus fermentum had antihypercholesterolemic potency. Keywords: antihypercholesterolemia, arrowroot yoghurt, fermentation, probiotic bacteriaABSTRAKSalah satu faktor penyebab penyakit kardiovaskular adalah hiperkolesterolemia. Altenatif untuk mencegah hiperkolesterolemia adalah mengonsumsi yoghurt umbi garut probiotik hipokolesterolemik. Salah satu probiotik yang mempunyai potensi hipokolesterolemik adalah Lactobacillus plantarum dan Lactobacillus fermentum. Tujuan penelitian ini menentukan formulasi kultur yang tepat pada produksi bahan minuman probiotik dari umbi tanaman garut yang memiliki aktivitas menurunkan kadar kolesterol darah.  Umbi garut difermentasi menggunakan formulasi kultur yang berbeda, terdiri dari A (Lactobacillus plantarum: Lactobacillus fermentum), B (Lactobacillus plantarum), dan C (Lactobacillus fermentum). Yoghurt umbi garut dievaluasi total asam laktat, pH, total bakteri asam laktat, organoleptik, dan diuji penurunan kadar kolesterol total secara in vivo. Formula yoghurt umbi garut terbaik menggunakan kultur C (Lactobacillus fermentum) berdasarkan parameter nilai total asam laktat, pH, dan total bakteri asam laktat. Setelah dilakukan uji penurunan kadar kolesterol total, maka yoghurt umbi garut yang difermentasi oleh Lactobacillus fermentum dan kontrol positif (simvastatin 0,18 mg/200 g bobot badan) dapat menurunkan kadar kolesterol total dengan persentase penurunan sebesar 30,36% dan 27,13%. Oleh karena itu, yoghurt umbi garut yang difermentasi oleh Lactobacillus fermentum berpotensi sebagai agen antihiperkolesterolemia.Kata kunci: antihiperkolesterolemia, bakteri probiotik, fermentasi, yoghurt umbi garut
FORMULATION OF INULIN FIBER SUPPLEMENT PRODUCED BY HYDROLYZING ASPERGILLUS CLAVATUS-CBS5 INULINASE ENZYME USING LOW-FAT SHANK GELATIN AS CHOLESTEROL BINDER Agustine Susilowati; Aspiyanto -; Hakiki Melanie; Yati Maryati
Biopropal Industri Vol 12, No 2 (2021)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v12i2.7059

Abstract

The formulation of low-fat shank gelatin and inulin hydrolysate as fructooligosaccharides (FOS) was conducted to obtain dietary fiber supplement as cholesterol binder. FOS was obtained through the hydrolysis of Aspergillus clavatus CBS5 fungi inulinase enzyme on commercial inulin. This study aimed to evaluate the ability of low-fat shank gelatin as a cholesterol binder in its application as inulin fiber supplement with a comparison of commercial gelatin. Application in preparing supplement was carried out at gelatin concentration of 30% (v/w hydrolysate) fortified on inulin fiber with 20, 40, 60, 80 and 100% (v/w total fiber). The experiment showed that fat reduction in crude shank gelatin tends to decrease composition and gel strength. A high concentration of inulin fiber would increase supplement composition on total solids, total sugar and reducing sugar and produced optimization of Soluble Dietary Fiber (SDF), Insoluble Dietary Fiber (IDF) and Cholesterol Binding Capacity (CBC). Based on optimum inulin fiber concentration and CBC, the best supplement formulation was achieved at inulin fiber of 60% with compositions of reducing sugar 99.5 mg/mL, total sugar 322.18 mg/mL, total solids 28.4%, SDF 8.33% (dry weight), IDF 21.783% (dry weight), CBC pH 7 (20.62 mg/g) and CBC pH 2 (16.65 mg/g). In this condition, shank gelatin increased CBC pH 2 by 11.56% and CBC pH 7 by 13.71% compared to commercial gelatin.Keywords: inulin fiber, formulation, supplement, gelatin, CBC ABSTRAKFormulasi gelatin ceker ayam rendah lemak yang dihasilkan melalui proses reduksi lemak dan hidrolisat inulin sebagai fructooligoccharides (FOS) dilakukan untuk memperoleh suplemen serat sebagai pengikat kolesterol. FOS diperoleh melalui hidrolisis enzim inulinase fungi Aspergillus clavatus CBS5 pada inulin komersial. Penelitian ini bertujuan untuk mengetahui kemampuan gelatin ceker ayam rendah lemak sebagai pengikat kolesterol dalam aplikasinya sebagai suplemen serat inulin dengan gelatin komersial sebagai pembanding. Aplikasi dalam pembuatan suplemen dilakukan pada konsentrasi gelatin 30% (v/w hidrolisat) yang difortifikasi pada serat inulin dengan konsentrasi masing-masing 20, 40, 60, 80 dan 100% (v/wtotal fiber). Hasil penelitian menunjukkan bahwa reduksi lemak pada gelatin ceker ayam cenderung menurunkan komposisi dan kekuatan gel. Semakin tinggi konsentrasi serat inulin akan meningkatkan komposisi suplemen terhadap total padatan, total gula dan gula reduksi, namun menghasilkan optimisasi SDF, IDF dan CBC. Berdasarkan efesiensi penggunaan serat inulin dan CBC maksimal, formulasi suplemen terbaik dicapai pada serat inulin 60% yang menghasilkan formula suplemen dengan komposisi gula reduksi 99,5 mg/mL, total gula 322,18 mg/mL, total padatan 28,4%, IDF 21,783 % (berat kering), SDF 8,33 % (berat kering), CBC pH7 20,62 mg/g dan CBC pH2 16,65 mg/g. Pada kondisi optimum dengan serat inulin 60%,  penggunaan gelatin ceker ayam meningkatkan CBC pH2 sebesar 11,56% dan CBC pH7 sebesar 13,71% dibandingkan dengan penggunaan gelatin komersial.Kata kunci: serat inulin, formula, suplemen, gelatin, CBC
POTENSI AKTIVITAS ANTIMALARIA DARI EKSTRAK AIR DAUN JUNG RAHAB (BAECKEA FRUTECENS) (Potency of Antimalarial Activity from Aqueaous Extract of Jung Rahab Leaves (Baeckea frutecens)) Yatri Hapsari; Eris Septiana; Fauzy Rachman; Syamsiah Syamsiah; Wien Kusharyoto; Leny Heliawati; Bustanussalam Bustanussalam; Siti Irma Rahmawati; Fauzia Nurul Izzati; Partomuan Simanjuntak
Biopropal Industri Vol 12, No 2 (2021)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v12i2.7074

Abstract

Malaria is a disease caused by Plasmodium parasite transmitted by female Anopheles mosquito. Jung rahab plant (Baeckea frutecens) is known have an ability to inhibit the growth of Plasmodium falciparum. However, the inhibition of the parasite growth is still unknown. This research aimed to determine fractionation of aqueaous extract of jung rahab leaves that is contain antimalaria compound with a potential inhibition activity. Jung rahab plant was extracted using decoctition. The analysis included phytochemical screening, column chromatography fractionation, antimalaria activity, and compound identification by using UV-Vis, FT-IR, and GCMS. Jung rahab aqueous extract fraction resulted a potential antimalaria activity with IC50 105,9 ppm and fractionation result based on UV-Vis, FT-IR, and GCMS as pyrogallol.Keywords: antimalarial, β-hematin, Baeckea frutecens, extraction, IC50ABSTRAKMalaria merupakan penyakit yang disebabkan oleh parasit Plasmodium yang ditransmisikan oleh nyamuk Anopheles betina. Daun jung rahab (Baeckea frutecens) diketahui memiliki kemampuan untuk menghambat pertumbuhan Plasmodium falciparum. Akan tetapi, mekanisme penghambatan pertumbuhan parasit tanaman ini masih belum diketahui. Penelitian ini bertujuan untuk mencari fraksi lain dari ekstrak air daun jung rahab yang mengandung senyawa antimalaria dengan aktivitas penghambatan yang lebih tinggi. Ektraksi daun jung rahab dilakukan dengan metode dekoktasi, sedangkan analisis yang dilakukan adalah skrining fitokimia, fraksinasi menggunakan kromatografi kolom, uji aktivitas antimalaria, dan identifikasi senyawa hasil fraksinasi dengan UV-Vis, FT-IR, dan GCMS. Berdasarkan hasil penelitian ini, fraksi ekstrak air daun jung rahab memiliki aktivitas antimalaria potensial dengan nilai IC50 sebesar 105,9 ppm dan hasil fraksinasi senyawa berdasarkan UV-Vis, FT-IR, dan GCMS sebagai pirogalol.Kata kunci: antimalaria, β-hematin, Baeckea frutecens, ekstraksi, IC50­

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