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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 10 Documents
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Search results for , issue "Vol 3, No 1 (2013)" : 10 Documents clear
Biomass production and antibacterial activity of Justicia gendarussa Burm. f. - A valuable Medicinal plant Sugumaran, P; Kowsalya, N; Karthic, Raju; Seshadri, S
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Rooting and biomass production of Justicia gendarussa has been achieved through a hydroponic system of cultivation. The obtained biomass of leaves, stem and root were examined for antibacterial activity against various human pathogenic organisms such as Staphylococcus aureus, Escherichia coli, Shigella sp., Pseudomonas sp. and Klebsiella pneumoniae. Methanolic extract of J. gendarussa root responded against E. coli. The growth of Shigella sp., Pseudomonas sp. and K. pneumonia were inhibited by leaf extract. The maximum inhibition zone against S. aureus was observed in stem extract treatment.
The Technic of Agroforestry at Communal Forest Areas in Pejarakan Village, District of Gerokgak, Regency of Buleleng, Province of Bali Ardhana, I putu Gede; Sunardi, Sunardi
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Abstract

Since 2003 year the technic of agroforestry with alley cropping systems has been implemented at the forest areas in Pejarakan Village, District Gerokgak, Regency of Buleleng Province of Bali. These activities are expected to support the progress of welfare for farmers, eventhough they have been neglected to preserve forest around village. After the research it was cleared that the result of activities didnt fulfilled to its expectation. The analysis about the compositions and structures of vegetations in this research shows that the dominant type of species which are mainly cultivated have character of Importance Value Index, the balance of species among Diversity Index are still low at all of the communal forest areas and even at the these slope sides. The composition and the structure of species were still few, and the vegetation itself was disturbed by people, in order to illegal loggings, cuttings top branches or twigs often occur, and these activities bring out obstacle growth of trees in communal forest areas. It is concluded that the technic of agroforestry at the communal forest areas has few compositions of species and structure of vegetations, so the diversity of species become very poor and not stable either for plants in forestry or for agricultural crops. The researcher could not determine that what species of vegetations superior at this area. So it necessary to enough time for intensive observations and research at this communal forest areas.
A Comparison Between Hyclone And Biowest: Effects To Human MSCs Proliferation, Morphology, And Osteogenic Differentiation Saskianti, Tania; Misami, Kanawa; Kato, Yukio
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Abstract

Mesenchymal stem cells (MSC) are expanded in a basal culture medium supplemented with fetal bovine serum (FBS) with or without additional growth factors. The serum contains basic components such as hormones and growth factors which provide robust MSC proliferation. However, the effects of different serum in the isolation and proliferation of primary MSC remains unclear. In this study we compared effect of serums on stimulating MSC proliferation and osteogenic differentiation. Therefore we evaluated the impact of Hyc1 and Bw1 containing medium on primary MSC morphology. Primary MSC grown in both serum containing medium retain the ability to differentiate into osteoblast. Bw1 containing serum showed slightly, unsignificanty higher potential as compared to Hyc1. However more detailed analysis on the composition of each serum on overall MSC morphology and proliferation must be further explored. The result of this study should form a basis for further studies examining specific substance needed in MSC proliferation and differentiation in more detail.
Survey on the use of synthetic Food Colors in Food Samples procured from different educational institutes of Karachi city. Saleem, Nida; Umar, Zahida Nasreen; khan, Seema Ismat
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Abstract

The present study was carried out to find the type of food colors added to various food products especially those vended at or near different educational institutes of Karachi city. Different types of food items were analysed for isolation and identification of the added synthetic food colors. The majority of branded food items contained permitted colors however some foods manufactured locally, contained non-permitted colors. About 11% branded and 44% unbranded food items, respectively, were found with not permitted colors for human consumption. Similarly, 4% branded and 30% unbranded beverages were found unfit due to the presence of prohibited colors. Incidences of the use of non-permitted food colors were higher in case of unorganized food makers. Constant vigilance is needed to ensure that the local manufacturers comply with the regulations of food colors not only in terms of non-permitted food colors but also about the control and limits of permitted food colors. Moreover, consumption of colored food items should also be controlled by making the society aware of the hazardous effects of food colors.
BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues fajri, wahyu nur laili; Rofi'i, Ahmad; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Abstract

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with α = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.
Exploratory Evaluation of Retranslocation and Bioconcentration of Heavy Metals in Three Species of Mangrove at Las Cucharillas Marsh, Puerto Rico Mejías, Carla Lorraine; Musa, Juan Carlos; Otero, Juan
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Abstract

Heavy metal contamination in the coastal area of Cataño, Puerto Rico accountable to anthropogenic sources is of great concern due to the risk posed over the surrounding communities and adjacent ecosystems. Estuarine ecosystems are widely recognized for the presence of mangroves. This type of flora is recognized for their many beneficial properties for example, the ability to purge aquatic ecosystems where they stand. Exploratory analyses on the phytoaccumulative capacity of three mangrove species for ten metals (Hg, Al, As, Cd, Cr, Cu, Fe, Pb, Mg, Zn) were performed in this research. Random soil samples and both green and senescent leaves of Rhizophora mangle, Laguncularia racemosa and Avicennia germinans from three different sites of Peninsula La Esperanza were analyzed in order to apply the retranslocation efficiency (RT%) and bioconcentration factor (BCF) concepts. After calculating the RT% and the BCF, comparison analyses among the three mangrove species were performed. In general, the results showed low RT% values for Avicennia in comparison with Rhizophora and Laguncularia. BCF values confirmed RT% results for Avicennia, showing higher heavy metal concentrations in its senescent leaves in contrast with the other species. Therefore, these preliminary results suggest that Rhizophora and Laguncularia act better as phytoremediators for heavy metals in polluted areas due to their ability to accumulate lower concentrations in senescent leaves; preventing further contamination in surrounding ecosystems by encapsulating the pollutants instead of exporting them.
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Wulandari, Dian Ratri; Habibi, Muhammad; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
Alteration of Coral Reef Community Structure Caused by Athropogenic Activity in Coastal Area of Giliketapang Island, Probolinggo, East Java, Indonesia Puspitasari, Lutfiatul; Samino, Setijono; Retnaningdyah, Catur
Journal of Tropical Life Science Vol 3, No 1 (2013)
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The aim of this research was to know the effect of anthropogenic activity around the coastal Giliketapang Island to community structure of coral reef. This research used belt transect method which consist of 18 transects in three location (harbour,near the forest and transition areas)at 50-80 m from the coast, with plot size of 2x2m2. Abundance of coral reefs were observed base on the coverage. There are found 11 families of coral reefs. The percentage of coral damage was decrease and the number of species was increase at the far zone from coast. This is also affect the coverage value of coral reefs. Coral coverage at the harbour and near the forest was 53. 06 % and 65. 36 % respectively, whereas in the transition area was 38. 38%. Co-domination between Porites lobata and Chyphastrea microphthalma occurred at the harbour, while Porites mayeri and Acropora spp. co-dominate in near the forest location. Poritidae family dominate in the transition area. Anthropogenic activities at transition location cause severed damage of coral reefs.
Formaldehyde And Benzo(Α)Pyrene induced Cells Necrotic in Mice Liver Soni, Ahmad; Widyarti, sri; Suwondo, Aris
Journal of Tropical Life Science Vol 3, No 1 (2013)
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Formaldehyde and benzo(α)pyrene are compounds that harmful for health. Misapplication of this compound have an impact in the form of organ damage in the body. This study aims to determine the impact of the treatment of the combined exposure of formaldehyde and benzo(α)pyrene to cell necrosis in the liver of mice (Mus musculus). Treatment of formaldehyde dose of 25 mg/kg BW to mice were given ​​orally every day for 60 days. Treatment of benzo(α)pyrene via intraperitoneal injection at a dose of 250 mg/kg BW were given after 30 days of incubation with four times injection with one day interval. Liver organ histological preparations were made through the HE staining. Observations were made by using a microscope for liver organ preparations. The data obtained that is the percentage of cells necrosis and necrotic foci. This research used Completely Randomized Design (CRD) with 95% confidence interval. Liver organ preparations observations indicate that the percentage of necrosis in the untreated control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, combination of formaldehyde 25 mg/kg BW with BaP in a row that is equal to 14.43% ± 0.91; 26.05% ± 3.75; 49.38% ± 2.66; 51.86 ± 1.73. The mean of necrotic foci in liver organ formed in the untreatment control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, and the combination of formaldehyde 25 mg/kg BW with BaP in a row, equal to 1.3 ± 0,07; 1.63 ± 0.61; 2 ± 0.51, and 3.4 ± 0.76. This suggests that the combined treatment had the highest level of toxicity compared with other treatments.
Accumulation of Calcium Crystal in Rat (Rattus norvegicus) Kidney Rosidah, A'liyatur; Widyarti, Sri; Rahayu, Sri
Journal of Tropical Life Science Vol 3, No 1 (2013)
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