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BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues fajri, wahyu nur laili; Rofi'i, Ahmad; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with Î± = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.

PENERAPAN PENDIDIKAN KARAKTER MELALUI PEMBELAJARAN IPS MENGGUNAKAN METODE PEMBELAJARAN DARING DI MTs NU MIFTAHUL ULUM LORAM KULON KECAMATAN JATI KABUPATEN KUDUS TAHUN 2020/2021 Hakim, Alief Lukman; Muthohar, Habib; Rofi'i, Ahmad
IJTIMAIYA: Journal of Social Science Teaching Vol 4, No 2 (2020): IJTIMAIYA : Journal of Social Science Teaching
Publisher : Program Studi Tadris IPS Fakultas tarbiyah IAIN Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/ji.v4i2.8581

Penelitian karakter ini berdasakan pada pembelajaran yang dilakukan di MTs NU Miftahul Ulum yang mana dalam pembelajran ini dilakukan daring. Proses pelaksanaan penelitiaan ini akan menggunakan metode kualitatif deskriptif untuk menghasilkan kenyataan karakter yang terbentuk dalam pembelajaran di Mts NU Miftahul Ulum. Penggalian ilmu peneliti menggunakan teknik wawancara dan pengamatan yang hasilnya menunjukan adanya signifikan pada karakter pembelajran yang dilakukan oleh MTs NU Miftahul Ulum. Bentuk dari pembelajaran yang dilaksanakan dalam masa daring bisa dilakukan untuk pendidikan karakter. Tidak hanya itu, proses pembelajaran ini daring masih bisa dilakukan seperti pada proses pembelajran secara konvensional.

PENERAPAN PENDIDIKAN KARAKTER MELALUI PEMBELAJARAN IPS MENGGUNAKAN METODE PEMBELAJARAN DARING DI MTs NU MIFTAHUL ULUM LORAM KULON KECAMATAN JATI KABUPATEN KUDUS TAHUN 2020/2021 Hakim, Alief Lukman; Muthohar, Habib; Rofi'i, Ahmad
IJTIMAIYA: Journal of Social Science Teaching Vol 4, No 2 (2020): IJTIMAIYA : Journal of Social Science Teaching
Publisher : Program Studi Tadris IPS Fakultas tarbiyah IAIN Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/ji.v4i2.8581

Penelitian karakter ini berdasakan pada pembelajaran yang dilakukan di MTs NU Miftahul Ulum yang mana dalam pembelajran ini dilakukan daring. Proses pelaksanaan penelitiaan ini akan menggunakan metode kualitatif deskriptif untuk menghasilkan kenyataan karakter yang terbentuk dalam pembelajaran di Mts NU Miftahul Ulum. Penggalian ilmu peneliti menggunakan teknik wawancara dan pengamatan yang hasilnya menunjukan adanya signifikan pada karakter pembelajran yang dilakukan oleh MTs NU Miftahul Ulum. Bentuk dari pembelajaran yang dilaksanakan dalam masa daring bisa dilakukan untuk pendidikan karakter. Tidak hanya itu, proses pembelajaran ini daring masih bisa dilakukan seperti pada proses pembelajran secara konvensional.

PENERAPAN PENDIDIKAN KARAKTER MELALUI PEMBELAJARAN IPS MENGGUNAKAN METODE PEMBELAJARAN DARING DI MTs NU MIFTAHUL ULUM LORAM KULON KECAMATAN JATI KABUPATEN KUDUS TAHUN 2020/2021 Hakim, Alief Lukman; Muthohar, Habib; Rofi'i, Ahmad
IJTIMAIYA: Journal of Social Science and Teaching Vol 4, No 2 (2020): IJTIMAIYA : Journal of Social Science and Teaching
Publisher : Program Studi Tadris IPS Fakultas tarbiyah IAIN Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/ji.v4i2.8581

Penelitian karakter ini berdasakan pada pembelajaran yang dilakukan di MTs NU Miftahul Ulum yang mana dalam pembelajran ini dilakukan daring. Proses pelaksanaan penelitiaan ini akan menggunakan metode kualitatif deskriptif untuk menghasilkan kenyataan karakter yang terbentuk dalam pembelajaran di Mts NU Miftahul Ulum. Penggalian ilmu peneliti menggunakan teknik wawancara dan pengamatan yang hasilnya menunjukan adanya signifikan pada karakter pembelajran yang dilakukan oleh MTs NU Miftahul Ulum. Bentuk dari pembelajaran yang dilaksanakan dalam masa daring bisa dilakukan untuk pendidikan karakter. Tidak hanya itu, proses pembelajaran ini daring masih bisa dilakukan seperti pada proses pembelajran secara konvensional.

PENGEMBANGAN SISTEM MEKANIKA DAN DINAMIKA PADA ROBOT EDUKASI BERBASIS IOT DAN ESP32 Rofi'i, Ahmad; Purnomo, Fendik Eko; Nari, Muhammad Irwan; Kautsar, Syamsiar; Winardi, Aditya Wahyu; Putri, Salsabila Liandra; Afianah, Nuzula; Fanani, Nurul Zainal; Alfiansyah, Faisal Luthfi; Koesacep, HAP; Nafsiyah, Haniatun; Kindi, Muhammad
Transmisi: Jurnal Ilmiah Teknik Elektro Vol 27, No 1 Januari (2025): TRANSMISI: Jurnal Ilmiah Teknik Elektro
Publisher : Departemen Teknik Elektro, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/transmisi.27.1.64-69

Fokus penelitian adalah mengembangkan mekanika dan dinamika pada robot edukasi berbasis IoT dan ESP32. Selain juga untuk memperkenalkan robotika sebagai media yang efektif dalam pembelajaran. Robot ini memiliki sistem penggerak diferensial dan lengan pengangkat yang mirip dengan sistem forklift. Robot ini dikontrol menggunakan mikrokontroler ESP32 dan terhubung ke aplikasi Android melalui basis data cloud Firebase. Dengan memanfaatkan platform IoT, pelatihan dapat dilakukan secara daring, yang memungkinkan siswa untuk mengendalikan robot dari jarak jauh saat robot berada di lokasi pelatihan. Robot pengangkut dirancang untuk memberikan pengalaman belajar robotika yang interaktif dan langsung, yang memungkinkan siswa untuk memahami konsep dasar dalam robotika, pemrograman, dan sistem kontrol. Sistem penggerak diferensial menawarkan kemampuan manuver yang sangat baik, sementara lengan pengangkat memungkinkan robot untuk melakukan tugas-tugas seperti mengangkat dan memindahkan objek, yang menambahkan aspek fungsional pada proses pembelajaran. Hasil pengujian menunjukkan bahwa robot bekerja dengan baik di bawah kendali daring, dengan respons yang cepat dan akurat terhadap perintah dari aplikasi Android. Penggunaan Firebase memastikan komunikasi yang stabil dan waktu nyata antara robot dan aplikasi. Implementasi ini tidak hanya meningkatkan aksesibilitas pelatihan robotika tetapi juga memungkinkan pengembangan keterampilan dengan cara yang lebih fleksibel dan terjangkau. Studi ini menyimpulkan bahwa robot transporter berbasis ESP32 dengan kontrol daring melalui aplikasi Android dan basis data cloud Firebase dapat menjadi alat pembelajaran yang efektif dan inovatif. Ini mendukung pengembangan keterampilan teknis dan pemahaman praktis siswa tentang robotika, menyediakan landasan yang kuat untuk studi lebih lanjut dalam teknologi dan teknik. Kinerja robot yang menjanjikan dalam pengaturan kontrol daring menyoroti potensinya sebagai sumber daya pendidikan yang berharga dalam lingkungan pembelajaran modern

Optimazion Analysis of Temperature and Fermentation Controlled Chamber (TFCC) with Hybrid System to Control Fermentation on Tape Rofi'i, Ahmad; Purnomo, Fendik Eko; Afriansyah, Faisal Luthfi
IJIEM - Indonesian Journal of Industrial Engineering and Management Vol 5, No 3: October 2024
Publisher : Program Pascasarjana Magister Teknik Industri Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22441/ijiem.v5i3.23373

The aim of this research is to determine analysis for optimizing tape fermentation using a Temperature and Fermentation Controlled Chamber (TFCC) with a Hybrid System. The resulting numerical model was analyzed using a non-linear differential method using Runge-Kutta. The thing that was analyzed was the change in the rate of yeast concentration, the rate of change in the concentration of ethanol and glucose resulting from the fermentation process. TFCC with Hybrid System Technology is equipped with an automated system accompanied by sensors that detect food quality and can be directly observed through the LCD screen integrated in the device. The research method used is experimental research by designing a tool that can be used to produce/cook fermented foods while monitoring pH, alcohol, glucose levels and temperature levels which are integrated in a set of tools called the TFCC with Hybrid Systems Technology . From the numerical explanation, it can be seen that when t is small, the rate of yeast development in the fermentation process will be small, the number of yeast cells is still small. As a result, the growth rate will be slow. This can be seen from the graph of the increase in X(t) and P(t) as well as the graph of S(t) which is still slow. The greater the time t, the rate of yeast consumption is also greater because the number of yeast cells is also increasing. As a result, the growth rate of ethanol and sugar is also large. This can be seen from the graph of an increase in X(t) and P(t) as well as a graph of a faster decline in S(t). When the maximum consumption rate is reached, then the values of X(t), S(t) and P(t) are constant. The amount of concentration in the resulting ethanol is close to the initial concentration of sugar produced.

Development of Robotics Learning Modules based on IoT and ESP32 Rofi'i, Ahmad; Afianah, Nuzula; Kautsar, Syamsiar; Pratama, Aditya Wahyu; Winadi, Aditya Wahyu; Purnomo, Fendik Eko; Putri, Salsabila Liandra; Tyagita, Dicky Adi; Nari, Mochammad Irwan; Afriansyah, Faisal Lutfi
International Journal of Technology, Food and Agriculture Vol. 2 No. 3 (2025): October (In Progress)
Publisher : P3M Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/tefa.v2i3.5885

This study aims to develop a transporter robot as a learning tool for elementary and secondary school students to introduce them to robotics. The robot features a differential drive system and a lifting arm akin to a forklift system. The robot is controlled using an ESP32 microcontroller and connected to an Android application through a Firebase cloud database. Utilizing an IoT platform, the training can be conducted online, allowing students to control the robot remotely while it remains at the training location. The transporter robot is designed to provide an interactive and hands-on learning experience in robotics, enabling students to grasp fundamental concepts in robotics, programming, and control systems. The differential drive system offers excellent maneuverability, while the lifting arm allows the robot to perform tasks such as lifting and moving objects, adding functional aspects to the learning process. Test results indicate that the robot performs well under online control, with fast and accurate responses to commands from the Android application. The use of Firebase ensures stable and real-time communication between the robot and the application. This implementation not only enhances the accessibility of robotics training but also enables the development of students' STEM skills in a more flexible and affordable manner. The study concludes that an ESP32-based transporter robot with online control via an Android application and Firebase cloud database can be an effective and innovative learning tool. This tool aims to enhance students’ STEM skills -especially in programming, control systems, and mechanical design- through interactive, real-world applications.

Analisis Desain Incinerator Pirolysis Fuel Reactor (IPFR) Limbah Plastik Sebagai Bahan Bakar Alternatif Wahyu Pratama, Aditya; Rofi'i, Ahmad; Afianah, Nuzula; Putri, Salsabila Liandra
Jurnal Teknik Terapan Vol. 2 No. 1 (2023): April
Publisher : P3M Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jteta.v2i1.5

In Indonesia, plastic waste has become an important problem at this time, because it causes environmental pollution problems. With this, further action is needed regarding the treatment of waste utilization, one of which is by converting waste into liquid fuel. Some types of rubbish that we often encounter are plastic bottles such as plastic cups, plastic bottle caps, children's toys and margarines and others, which is one of the types of PP (polypropylene) waste. The development of current plastic waste treatment technology is to convert plastic waste into fuel oil by utilizing plastic waste to be processed as plastic fuels such as premium fuel, diesel and kerosene. Polypropylene type plastic is the type of plastic that is most widely used in daily life because it has good mechanical properties with low density, heat and moisture resistance, and has good dimensional stability. Some of the technology used to convert plastic waste, among others, by using the method of high temperature (Thermal Cracking). In this study, researchers designed a Thermal cracking device, namely an Incinerator, a plastic waste smelter with high temperature, where the modification of this tool is to use used fuel oil or residual oil. In this study, the researcher analyzed the burning time, the amount of waste mass needed for the melting process in the Incinerator. In this research, a fuel equivalent of 1.5 liters of premium fuel was produced with 10 kg of plastic waste burned in the incinerator with a burning time of 125 minutes of the pyrolysis process, with a combustion temperature of 180-250 degrees Celsius.

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