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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol. 5 No. 3 (2015)" : 9 Documents clear
Isolation and Characterization Compounds From Hexane and Ethyl Acetate Fractions of Peperomia pellucida L. Sri Hartati; Marissa Angelina; Indah Dewiyanti; Lia Meilawati
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.02

Abstract

      Peperomia pellucida was used traditionally in Indonesia for health treatment: wounds, boils, pimples, abscesses, abdominal pain, colic, gout, kidney, rheumatic pain, fatigue headache, furuncles, conjunctivitis and anti dermatogenic and also for dengue treatment. The isolation compounds from hexane and ethyl acetate fractions of Peperomia pellucida L. are  conducted by maceration of the dry herbs sample with methanol and partition with hexane, ethyl acetate, butanol and water.The hexane and ethyl acetate fractions were fractionated by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by the gradient. The structure was elucidated base on spectroscopy data of NMR proton and carbon for one and two dimension, LC-MS and FT-IR. The isolation founded three compounds are stigmasterol, analogue of pheophytin and b-sitosterol-D-glucopyranoside.
Bacteria as Potential Indicators of Heavy Metal Contamination in a Tropical Mangrove and the Implications on Environmental and Human Health Melanie De La Rosa- Acosta; Johannys Jiménez-Collazo; Marixa Maldonado-Román; Karlo Malavé-Llamas; Juan Carlos Musa-Wasil
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.01

Abstract

Heavy metal (HM) exposure has been associated with human health diseases like cancer, kidney and liver damage, neurological disorders, motor skills, low bone density and learning problems. With the beginning of the industrialization the heavy metals in high concentration contributes to put on risk the humans in the vicinity. Our study site is located in Cataño, Puerto Rico, a highly industrialization area that has a recreational park nearby, a rum distillery, two thermoelectric factories, and was impacted by CAPECO (oil refinery) explosion in 2009. Las Cucharillas marsh is part of The San Juan Bay Estuary System, considered as a critical wildlife area because of their location. This mangrove marsh has three of the four mangrove species found in PR Laguncularia racemosa, Avicennia germinans and Rhizophora mangle; species that have the capacity to phytoremediate HM. This study was aimed at seven different heavy metals: Arsenic (As), Cadmium (Cd), Chromium (Cr), Lead (Pb), Zinc (Zn), Mercury (Hg) and Copper (Cu). These metals at high concentrations are of human health concern due to their toxicity, persistence, bioaccumulative and biomagnification potentials. Contamination of surface sediments with HM affects the food chain, starting with marine organisms up to humans. The people who live near the contaminated area and the local fishermen are at high risk of exposure. Studies reveal that certain microorganisms can resist the toxicity of heavy metals even at high concentrations. Our study pretends to exploit the sensitive nature of some bacteria to HM and use them as bioindicators. The objective of this research is to assess the bacterial community on the mangrove marsh, identify these bacteria and correlate bacterial species with the type and concentration of the metals found on the site. Our preliminary results with the BIOLOG® identification were five bacteria that are: Carnobacterium inhibens, Cupriavidus gilardii, Enterococcus maloduratus, Microbacterium flavescens and Ralstonia pickettii. This study will continue with an assessment of the exposure of different concentrations of heavy metals to our identified bacteria and underlying the mechanisms of degradation, magnification and or bioconcentration of these heavy metals.
Cocoa extract has activity on selectively killing of breast cancer cells line ariza budi tunjung-sari; Mahriani Mahriani; Gusti Agung Perias Tiningrum; Teguh Wahyudi; Misnawi Jati
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.04

Abstract

Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 μg/ml. The positive control was doxorubicin (0.5-16 μg/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 μg/ml (MCF-7) and 1893 μg/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was0,777 μg/ml (MCF-7) and 0,082 μg/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 μg/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 μg/ml, however, 3-fold content resulted in slightly higher LC50 at 780 μg/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property .
Level Of 25(OH)D Serum, Expression of Interleukin 4 And Glucocorticoid Receptor of Mononuclear Cell In Steroid Resistance Nephrotic Syndrome Children Vonny Mariany Deckert; Loeki Enggar Fitri; Krsni Subandiyah
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.06

Abstract

Nephrotic syndrome (NS) is autoimmune disease and its steroid resistance status supposed correlate with 25(OH)D level and IL-4 expression. The aimed of this study was investigated 25(OH)D plasma level, IL-4 and GR expression of PBMC in steroid sensitive and resistant pediatric NS patients and the association of those parameters. 27 subjects were divided into three groups (control group, steroid resistant NS group, and steroid sensitive NS group). Peripheral blood mononuclear cells (PBMCs) isolated using Ficoll-Hypaque method. Plasma 25(OH)D level was measured using ELISA method. IL-4 and GR expression were measured using flowcytometry of PBMCs. This study showed that 25(OH)D level and GR expression were significantly different in control group compared to steroid resistant NS group (p<0.05). Plasma 25(OH)D level, IL-4 and GR expression were not correlated each other in NS patients (p>0.05). Plasma 25(OH)D level, IL-4 and GR expression were not contributed in steroid resistance in NS patients. However, GR expression has highest contribution in steroid resistance of NS patient (Wald score 1.198). Plasma 25(OH)D level and GR expression was lower in steroid resistant NS group. GR expression has a highest contribution in steroid resistance of NS patients.
A Field Survey To Investigate The Flora And Fauna of Dhauladhar Nature Park in North-Western Himalayan Region of India Sharma Kumar Neeraj
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.07

Abstract

Taxonomic studies on wildlife biodiversity has always been very fascinating along with the great purpose of providing valuable information on their present status concerning species number and future course of action required for their conservation. A study regarding survey of flora and fauna was carried out at Dhauladhar Nature’s Park of Gopalpur in district Kangra of Himachal Pradesh during the month of April 2015. The present study has revealed the presence of 09 mammals, 02 reptilia, 05 pheasants and 04 other birds belonging to 12 families along with 44 plant species belonging to 28 families. Further it is suggested that provisions for Himalayan snakes and butterflies, the other two imperative Himalayan fauna be created as soon as possible so that nature’s park can fulfill its  true purpose of conservation of Himalayan wild animals in real sense.
Spatial Variability of Fruit and Seedling Growth of Mindi (Melia azedarach L.) in Community Forest, West Java, Indonesia Laswi Irmayanti; Iskandar Zulkarnaen Siregar; Prijanto Pamoengkas
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.09

Abstract

Mindi (Melia azedarach L.) is mostly found in community forests of West Java. One of the important factors affecting productivity of community forests is the use of quality seed. Due to scarcity of seed sources, however, seed quality is not easily available. Currently, community forests serve also as ad hoc seed sources that are usually without appropriate quality assessment. This research was aimed to determine baseline information on spatial variability of mindi fruits collected from five seed sources, and seedling growths treated in seven different growing media consisting mixtures of soil, rice husk, sand and cattle manure. Five mindi populations were selected in the following villages: Nagrak (Bogor), Babakan Rema (Kuningan), Padasari (Sumedang), Sukakarya (Bogor) and Gambung (Bandung). The results showed that variability of seed dimension (CV=4.82-14.37%) and seedling growth (11.79-21.77%) were moderate. The mindi fruits from Nagrak were found to be the biggest in terms of size (1.22 ± 0.17 cm), weight (0.40 ± 0.04 g), and seed set (85.47 ± 15.09%). In addition, the seedling growth from Nagrak was also better than those of other villages showing its potential for future seed sources. Growing media containing cattle manure were observed consistently to be the best for seedling growth. The findings are useful in supporting scientifically the selection of seed sources from community forests.
The effect of Nigella Sativa extract on Alpha-ketoglutarate activity and histopathologic changes on Rat liver induced by Monosodium glutamate Ala Shukri Eshami; Karyono mitaroem; Hidayat Sujuti; Abdussalam Ashour
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.03

Abstract

         Monosodium glutamate (MSG) is a commonly used food additive and found in most soups, fish and processed meat. The use of MSG in food is growing. Irrational fear had increased in the last few years due to the adverse reactions and toxicity of MSG, which effect on the liver.  Nigella sativa is used as traditional medicine for the treatment of many diseases, it has been extensively investigated in recent years, traditional medicine for the treatment of many disease due to its notable pharmacological properties. NS can inhibit oxidative stress. The present study was undertaken to investigate different Doses of Nigella sativa on alpha KGDH activity and liver histology of MSG induced rat. The animals (n=30) Were grouped  A as (control), B treated with MSG 1g/kg, C MSG treated with NS 0.01g/kg, D MSG treated with NS 0.02g/kg, E MSG treated with NS 0.04g/kg and F treated with NS 0.02g/kg then we investigated the effect alpha KGDH activity by ELISA method and liver histopathology by light microscope.  alpha KGDH activity were significantly increased in treatment MSG compared with treatment negative control, MSG + NS 0.1g/kg, MSG + NS 0.02g/kg, MSG + NS 0.04g/kg, and NS 0.02g/kg. The histological changes in group B and C showed disturbed liver architecture, hemorrhage in the central veins, areas of necrosis, vacuolation and increased inflammatory cells infiltration while group D showed central vein is normal and vacuolation of cytoplasm, group E showed more normal liver architecture as shown less vacuolation in cytoplasm, central vein is normal, and group F showed no pathologic changes.These finding showed that administration of MSG increase alpha KGDH and induced damage in liver tissue. Nigella sativa extract can reduce alpha KGDH and prevent liver damage induced by MSG.Keywords :  Monosodium glutamate, Alpha-ketoglutarate Dehydrogenases, Nigella sativa and liver damage.
Modulation of Granulocyte Cells Development by VipAlbumin® Administration in BALB/C Mice with Diabetes Mellitus Andi Rizki Adi Pradana; Mansur Ibrahim; Muhammad Sasmito Djati; Muhaimin Rifa'i
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.05

Abstract

Diabetes mellitus is a metabolic disease that is caused either by the decrease of insulin secretion frompancreatic β cells or the insensitivity of target cells against insulin. High glucose levels (hyperglycemia condition)can trigger the formation of free radicals, the main cause of diabetes micro and macrovascular complications. Theformation of free radicals and AGE (advanced glycation end-products) is assumed to became the key factor in thedecline of granulocyte cell production as well as the disruption of these cells functional activity. The purpose ofthis research was to determine the role of VipAlbumin® in inhibiting the adverse effects of increased blood glucoselevels, which highly influence the production of granulocyte. This study was divided into in vitro and in vivostage. BALB/C mice were used as experimental animals at in vivo stage and induced to undergo diabetes through100 mg/kg BW streptozotocin (STZ) injection at the age of 5 days. VipAlbumin® administered orally for 14 days,which began when mice reached the age of 14 weeks. The administration of VipAlbumin® divided into 3 dosesi.e. 0,01664 mg/gr BW (1st dose), 0,416 mg/gr BW (2nd dose), and 10,4 mg/gr BW (3rd dose). The further step wasa flowcytometric analysis to see the development of granulocyte cells relative amount, which were isolated fromthe bone marrow. The result of this analysis shows that VipAlbumin® administration, particularly at the 2nd and3rd dose, were able to modulate granulocyte cells development in the bone marrow.
Recent status of Banteng (Bos Javanicus) conservation in East Java and its perspectives on ecotourism planning Luchman Hakim; Dodit Ari Guntoro; Joko Waluyo; Dian Sulastini; Lugi Hartanto; Nokukazu Nakagoshi
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.08

Abstract

The aims of this article are to examine the recent status of  Banteng Bos javanicus conservation in East Java, identify the roots of conservation problems and propose the non-consumptive and sustainable uses of Banteng by implementing ecotourism. Recently, Banteng population distributes in Alas Purwo, Meru Betiri, and Baluran National Parks.  The population in Alas Purwo and Meru Betiri were relatively stable yearly. Rapid population decrease found in Baluran National Park. The roots of threats may be categorized into two factors, socio-economic and ecological factors. Socio-economic problems lead to the increase of habitat disturbance, poaching, and illegal hunting. Ecological aspect was ranging from invasion of exotic plant species, competitors, predators, drought, forest fire and vegetation changes. Lack of habitat management also recognized as an important factor to drive Bos javanicus decline and extinction. Ecotourism in the national park may become one of the significant and effective stimuli to support Banteng conservation.

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