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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Jurnal Ilmu Dasar BERKALA SAINSTEK Biosfer: Jurnal Tadris Biologi UNEJ e-Proceeding BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOEDUKASI BIOEDUSCIENCE Biota
Mahriani Mahriani
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Jember, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Environmental Science Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Cocoa extract has activity on selectively killing of breast cancer cells line tunjung-sari, ariza budi; Mahriani, Mahriani; Tiningrum, Gusti Agung Perias; Wahyudi, Teguh; Jati, Misnawi
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.04

Abstract

Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 μg/ml. The positive control was doxorubicin (0.5-16 μg/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 μg/ml (MCF-7) and 1893 μg/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was0,777 μg/ml (MCF-7) and 0,082 μg/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 μg/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 μg/ml, however, 3-fold content resulted in slightly higher LC50 at 780 μg/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property .
DIURETICS EFFECT OF AVOCADO LEAF (Persea americana Mill.) ETHANOL EXTRACT ON THE GASTRIC HISTOLOGY OF WISTAR MALE RATS (Rattus norvegicus) Dwi Erlinda; Mahriani Mahriani; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i1.3007

Abstract

Irregular urine output triggers kidney stone disease. One way to cure it is by using synthetic diuretic compounds. The use of synthetic diuretic drugs for a long time can cause side effects, namely gastric damage. Therefore, research is needed to determine the diuretic effect of ethanol extract of avocado leaves (Persea americana) and its effect on the histological structure of the gastric  of male rats. Avocado leaf extract is made by maceration method, using 70% ethanol and then evaporated. Wistar male rats were divided into 5 treatment groups namely negative control (aquadest), positive control (furosemide), avocado leaf extract dose of 50 mg / kg body weight, 100 mg / kg body weight, and 150 mg / kg body weight with oral administration for 15 days. The volume of urine  was measured for 24 hours, then the mice were sacrified on the 16th day. Gastric organs were taken for histological observation by the paraffin method and Hematoxylin and Eosin (H&E) staining. The results showed that the optimum avocado leaf ethanol extract was a dose of 100 mg/kg showed a diuretic effect but did not affect the histological structure of the gastric.
EFFECT OF PERIWINKLE (CATHARANTHUS ROSEUS) LEAF EXTRACT ON LIVER HISTOLOGY OF MICE (MUS MUSCULUS L) AFTER ASPARTAME INDUCE Mahriani Mahriani; Rosita Dewi Wulandari; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 2 (2022): Februari 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.5161

Abstract

Aspartame is an artificial sweetener in foods and beverages. Continuous use of aspartame will result in oxidative stress in the liver. So we need antioxidants from outside the body such as periwinkle leaves.This study aims to determine the effect of periwinkle leaves (Catharanthus roseus L) on aspartame-induced liver histology. This study used 24 male mice divided into 4 groups, namely negative control (K-) without treatment; Positive control (K +) treatment of aspartame; D1: treatment of aspartame and periwinkle extract doses of 0.42 mg/kg. D2: aspartame and periwinkle  extract doses of 0.84 mg/kgbb. Administration of aspartame dose of 0.2 mg /kgbb by intraperitoneal  for 14 days followed by gavage administration of periwinkle leaf extract for 12 days. The liver was taken on the 27th day by surgery, then made slide histology with Hematoxylin-Eosin staining. The results show that the administration of Catharanthus roseus leaf extract at a dose of 0.84 mg/kgbw in aspartame-induced mice was effective in reducing hepatocyte damage, namely hydrophilic degeneration and necrosis.
Efek Ekstrak Daun Seledri (Apium graveolens L.) Terhadap Struktur Histologi Ginjal Tikus (Rattus norvegi Nana Zaimatul Husna; Mahriani Mahriani; Hidayat Teguh Wiyono
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p10

Abstract

The kidneys are an important excretory organ for removing the rest of the body's metabolism. Kidney stones can affect kidney function. Celery contains flavonoid compounds that can improve the histological structure of the kidneys. This study aims to determine the effect of celery extract on the histological structure of the kidneys, namely the diameter of the glomerulus and tubule with protein deposits. This research is experimental with completely randomized design (CRD). The treatment group was divided into 4 groups, namely, negative control (K-), rats were only given food and drink; positive control (K+), rats induced Ethylene glycol (EG) 0.75% + Ammonium Chloride (AK) 2%, dose 1 (D1), rats induced EG 0.75% + AK 2% and celery extract 100 mg / kg, dose 2 (D2), rats induced EG 0.75% + AK 2% and celery extract 200 mg / kgBB. Celery extract was given for 14 days by gavage. Celery extract at doses of 100 and 200 mg / kgBB were able to improve the histological structure of the rats' kidneys induced by EG 0.75% and AK 2%. Keywords: celery, glomerular diameter, protein deposits
KAJIAN EKSPRESI PROTEIN BAX PADA GANGGUAN SPERMATOGENESIS PASCA PEMAPARAN 2,5-HEXANADIONE, PADA TIKUS PUTIH (RATTUS NORVEGICUS L) MAHRIANI -
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.594 KB)

Abstract

The disturbance of spermatogenesis after exposure with 2,5-HD (2,5-Hexanedione) can be observed through the increasing of death cell in apoptosis mechanism, that can be observed through the expression of Bax protein. Therefore, it had been conducted a research to study the expression of Bax protein against white mouse of Wistar strain exposured with 2,5-HD. A number of 56 white mouse were divided randomly into 8 groups, each group consists of 7 white mouse, two groups were used as pretest, three groups used as control treatment, and three groups used as treatment were exposured with 2,5-HD of 0,06 mg/WW during 2, 4, 6 week. The changes in the Bax protein expression observed in the slices of cross section of testis using imunohistochemical method. The research result shows that the longer duration of 2,5-HD exposure causes the more increase the expression of Bax protein at the sertoli cell and the germinal. The expression of Bax protein compared to the control groups increases into 132%, 112%, 108% and 123% respectively for the sertoli, spermatogonial, spermatocyt, and spermatid cells in the treatment were exposured with 2,5-HD during 6 weeks. It is needed a further research to complete information of apoptosis mechanism as the result of 2,5-HD exposure, by study the ratio of Bcl-2/Bax expression as the antiapoptosis and proapoptosis proteins.
Efek ekstrak etanol kedelai hitam (Glycine soja) terhadap ketebalan dermis mencit (Mus musculus L.) pasca unilateral ovariektomi Isna Kurotul Akyun; Susantin Fajariyah; Mahriani Mahriani
Jurnal Biologi Udayana Vol 23 No 2 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.843 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i02.p05

Abstract

Estrogen is a steroid hormone produced by the ovaries. Estrogen is a hormone that triggers collagensynthesis by fibroblast.Collagen has affects for thickness of dermis.Estrogen deficiency can lead disruptioncollagen synthesis, so has an impact on decline dermis thickness. Unilateral ovariectomy is the act ofremoval of one ovary in the female reproductive system and is a model for estrogen deficiency. Thecondition of estrogen deficiency can be overcome by giving phytoestrogens. Black soybean seed is one ofphytoestrogens source. The purpose of this study was to determine the effect of black soybean extract afterovariectomy on increasing on dermis thickness (Mus musculus L.). The dosage of black soybean ethanolextract used was 0.31 g / ml / day and 0.63 g / ml / day administered orally (gavage) for 20 days. The resultsshowed a dose of 0.31 g / ml / day may and 0.63 g / ml / day increase dermis thickness of mice.
Efek pemberian ekstrak kedelai hitam (Glycine soja) terhadap histologi femur pada mencit (Mus musculus) ovariektomi mahhriani mahriani; Resa Miftahatu Yuniar; Masrurotul Hasanah; Eva Tyas Utami
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p05

Abstract

Bone is a dynamic structure and has the process to remodelling or regeneration. Bone remodelling includes the process of formation by osteoblasts and the process of resorption by osteoclasts. The balancing between formation and resorption are assisted by the estrogen hormone.Estrogen deficiency can lead the bone resorption. Ovariectomy is a model for estrogen deficiency by removing the ovaries. One way to overcome estrogen deficiency can be solved by offering phytoestrogens. This research have purpose to determine the effect of black soybean extract (Glycine soja) on the histology of the femur mice (Mus musculus) ovariectomized. In this research used a dose of black soybean extract as many as 0.42 g /ml/day and 0.63 g/ml/day, which are given orally for 30 days. The research is conducted with a completele randomized design with 4 treatments and 6 replications. Observations are made on the number of osteoblasts and the number of osteoclasts on the histological of the femur. The results showed that offering black soybean extract at a dose of 0.42 g/ml/day for 30 days have significantly increased the number of osteoblasts and have decreased the number of osteoclasts in the femur of ovariectomized mice.
THE EFFECT OF SOY TEMPEH FLOUR EXTRACT ON VAGINA HISTOLOGICAL STRUCTURE OF SWISS WEBSTER OVARIECTOMIZED MICE (MUS MUSCULUS) Mahriani Mahriani; Eva Tyas Utami; Dita Ayu Faradila
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Estrogen deficiency can changes the reproductive system especially in menopausal women, such as a decreasing of smooth muscle contraction, a thinning of vaginal structure, epithelial atrophy and a collagen decreasing. The disorders can be overcome by phytoestrogens treatment. Tempeh is a fermented soy product contains isoflavones that has estrogen-like structure with 17β-estradiol so that it can be able to bind estrogen receptors. Ovariectomized animal as animal test model has estrogen deficiency. Therefore, the purpose of this study to determine the effect of tempeh soy flour extract on vagina histological structure of Swiss Webster ovariectomized mice. The method used animals tested by treating 45 female Swiss Webster mice that were divided into five groups: negative control group (normal mice had not ovariectomized was not given tempeh flour extract), positive control (ovariectomized mice was not given tempeh flour extract), the treatment group (ovariectomized mice and was given tempeh flour extract D1 (0.21 g/ml / day), D2 (0.42 g/ml/day), and D3 (0,63 g/ml/day). Soy tempeh flour extract was given for 10, 20, and 30 days by gavage. The histological parameter were observed include tunica mucosa epithelium and tunica muscularis thickness of vagina. The result showed that tempeh flour extract increase epithelium thickness and tunica muscularis of the vagina ovariectomized mice. Treatment by taking longer time and giving higher dosage of tempeh flour extract could increase the ephitelium thickness of tunica mucosa and tunica muscularis vagina. The treatment of 0.63 g/ml/day dosage during 30 days showed the highest thickness average values of epithelium and tunica muscularis. The ephitelium thickness was 33.90 μm and the tunica muscularis dense was 90.20 μm.
THE EFFECT OF SOY TEMPEH FLOUR EXTRACT TO UTERINE HISTOLOGY OF OVARIECTOMIZED MICE Eva Tyas Utami; Mahriani Mahriani; Nidaul Hikmah
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Uterine is one of organs that affected by estrogen hormone. This hormone can influence to increase uterus cells proliferation. Decreasing ovum production cause decreasing of estrogen level. This situation is faced of menopause that affect on uterine development. Phytoestrogens can bind to estrogen receptor and produce estrogenic affects like endogeneous estrogen. Tempeh is known as soybean fermented and consists of high level of isoflavon as phytoestrogens. Phytoestrogens have structure like mammalian estrogen 17β-estradiol. The aim of this study was to know effects of tempeh soy fluor extracts on mice uterine strain swiss webster ovariectomized.. Dosages that used on this study are 0.21g/mL, 0.42 g/mL and 0.63g/mL. The dosages are treated by gavage during 10 and 20 days after ovariectomy.The result showed that tempeh soy fluor extracts can increase the average of uterine mass, endometrial and myometrium thickness during treatment on 10 and 20 days. The 0,63g/mL dosage increase the highest of uterine mass (0.043g), endometrium thickness (389.46μm) and myometrium thickness ( 71.30μm.).
Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure Mahriani Mahriani; Susantin Fajariyah; Eva Tyas Utami
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.196

Abstract

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.
Co-Authors Aminuyati ariza budi tunjung-sari ariza budi tunjung-sari, ariza budi Bella Dwi Arifianti Dewi, Efie Fadjrijah Eka Dita Ayu Faradila Dwi Erlinda Eva Tyas Utami Gusti Agung Perias Tiningrum Gusti Agung Perias Tiningrum, Gusti Agung Perias Hidayat Teguh Wiyono Indah Salsabila Kurnia Isna Kurotul Akyun Kartika Senjarini Kurnia, Indah Salsabila Masrurotul Hasanah Misnawi Jati Misnawi Jati Nana Zaimatul Husna Nidaul Hikmah Primasari, Nindita Fitria Resa Miftahatu Yuniar Rike Oktarianti Rosita Dewi Siti Agustina Wulandari Susantin Fajariyah Teguh Wahyudi Teguh Wahyudi Yenny Febriana Ramadhan Abdi