Garuda - Garba Rujukan Digital

Cocoa extract has activity on selectively killing of breast cancer cells line tunjung-sari, ariza budi; Mahriani, Mahriani; Tiningrum, Gusti Agung Perias; Wahyudi, Teguh; Jati, Misnawi
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.04

Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 Î¼g/ml. The positive control was doxorubicin (0.5-16 Î¼g/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37Â°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 Î¼g/ml (MCF-7) and 1893 Î¼g/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was0,777 Î¼g/ml (MCF-7) and 0,082 Î¼g/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 Î¼g/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 Î¼g/ml, however, 3-fold content resulted in slightly higher LC50 at 780 Î¼g/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property .

DIURETICS EFFECT OF AVOCADO LEAF (Persea americana Mill.) ETHANOL EXTRACT ON THE GASTRIC HISTOLOGY OF WISTAR MALE RATS (Rattus norvegicus) Dwi Erlinda; Mahriani Mahriani; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i1.3007

Irregular urine output triggers kidney stone disease. One way to cure it is by using synthetic diuretic compounds. The use of synthetic diuretic drugs for a long time can cause side effects, namely gastric damage. Therefore, research is needed to determine the diuretic effect of ethanol extract of avocado leaves (Persea americana) and its effect on the histological structure of the gastric of male rats. Avocado leaf extract is made by maceration method, using 70% ethanol and then evaporated. Wistar male rats were divided into 5 treatment groups namely negative control (aquadest), positive control (furosemide), avocado leaf extract dose of 50 mg / kg body weight, 100 mg / kg body weight, and 150 mg / kg body weight with oral administration for 15 days. The volume of urine was measured for 24 hours, then the mice were sacrified on the 16th day. Gastric organs were taken for histological observation by the paraffin method and Hematoxylin and Eosin (H&E) staining. The results showed that the optimum avocado leaf ethanol extract was a dose of 100 mg/kg showed a diuretic effect but did not affect the histological structure of the gastric.

EFFECT OF PERIWINKLE (CATHARANTHUS ROSEUS) LEAF EXTRACT ON LIVER HISTOLOGY OF MICE (MUS MUSCULUS L) AFTER ASPARTAME INDUCE Mahriani Mahriani; Rosita Dewi Wulandari; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 2 (2022): Februari 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.5161

Aspartame is an artificial sweetener in foods and beverages. Continuous use of aspartame will result in oxidative stress in the liver. So we need antioxidants from outside the body such as periwinkle leaves.This study aims to determine the effect of periwinkle leaves (Catharanthus roseus L) on aspartame-induced liver histology. This study used 24 male mice divided into 4 groups, namely negative control (K-) without treatment; Positive control (K +) treatment of aspartame; D1: treatment of aspartame and periwinkle extract doses of 0.42 mg/kg. D2: aspartame and periwinkle extract doses of 0.84 mg/kgbb. Administration of aspartame dose of 0.2 mg /kgbb by intraperitoneal for 14 days followed by gavage administration of periwinkle leaf extract for 12 days. The liver was taken on the 27th day by surgery, then made slide histology with Hematoxylin-Eosin staining. The results show that the administration of Catharanthus roseus leaf extract at a dose of 0.84 mg/kgbw in aspartame-induced mice was effective in reducing hepatocyte damage, namely hydrophilic degeneration and necrosis.

THE EFFECT OF SOY TEMPEH FLOUR EXTRACT ON VAGINA HISTOLOGICAL STRUCTURE OF SWISS WEBSTER OVARIECTOMIZED MICE (MUS MUSCULUS) Mahriani Mahriani; Eva Tyas Utami; Dita Ayu Faradila
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Estrogen deficiency can changes the reproductive system especially in menopausal women, such as a decreasing of smooth muscle contraction, a thinning of vaginal structure, epithelial atrophy and a collagen decreasing. The disorders can be overcome by phytoestrogens treatment. Tempeh is a fermented soy product contains isoflavones that has estrogen-like structure with 17β-estradiol so that it can be able to bind estrogen receptors. Ovariectomized animal as animal test model has estrogen deficiency. Therefore, the purpose of this study to determine the effect of tempeh soy flour extract on vagina histological structure of Swiss Webster ovariectomized mice. The method used animals tested by treating 45 female Swiss Webster mice that were divided into five groups: negative control group (normal mice had not ovariectomized was not given tempeh flour extract), positive control (ovariectomized mice was not given tempeh flour extract), the treatment group (ovariectomized mice and was given tempeh flour extract D1 (0.21 g/ml / day), D2 (0.42 g/ml/day), and D3 (0,63 g/ml/day). Soy tempeh flour extract was given for 10, 20, and 30 days by gavage. The histological parameter were observed include tunica mucosa epithelium and tunica muscularis thickness of vagina. The result showed that tempeh flour extract increase epithelium thickness and tunica muscularis of the vagina ovariectomized mice. Treatment by taking longer time and giving higher dosage of tempeh flour extract could increase the ephitelium thickness of tunica mucosa and tunica muscularis vagina. The treatment of 0.63 g/ml/day dosage during 30 days showed the highest thickness average values of epithelium and tunica muscularis. The ephitelium thickness was 33.90 μm and the tunica muscularis dense was 90.20 μm.

THE EFFECT OF SOY TEMPEH FLOUR EXTRACT TO UTERINE HISTOLOGY OF OVARIECTOMIZED MICE Eva Tyas Utami; Mahriani Mahriani; Nidaul Hikmah
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Uterine is one of organs that affected by estrogen hormone. This hormone can influence to increase uterus cells proliferation. Decreasing ovum production cause decreasing of estrogen level. This situation is faced of menopause that affect on uterine development. Phytoestrogens can bind to estrogen receptor and produce estrogenic affects like endogeneous estrogen. Tempeh is known as soybean fermented and consists of high level of isoflavon as phytoestrogens. Phytoestrogens have structure like mammalian estrogen 17β-estradiol. The aim of this study was to know effects of tempeh soy fluor extracts on mice uterine strain swiss webster ovariectomized.. Dosages that used on this study are 0.21g/mL, 0.42 g/mL and 0.63g/mL. The dosages are treated by gavage during 10 and 20 days after ovariectomy.The result showed that tempeh soy fluor extracts can increase the average of uterine mass, endometrial and myometrium thickness during treatment on 10 and 20 days. The 0,63g/mL dosage increase the highest of uterine mass (0.043g), endometrium thickness (389.46μm) and myometrium thickness ( 71.30μm.).

Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure Mahriani Mahriani; Susantin Fajariyah; Eva Tyas Utami
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.196

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.

THE IMMUNOGLOBULIN (IgG) ANALYSIS OF IMPLANTATION MICE (Mus musculus L.) POST OVARIECTOMY AFTER TREATMENT OF BLACK SOYBEAN FLOUR EXTRACT (Glycine soja) Yenny Febriana Ramadhan Abdi; Mahriani Mahriani; Kartika Senjarini; Rike Oktarianti
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.21099

Black soybean is one type of plant that has estrogenic activity, it can act as a source of natural exogenous estrogen. Decreased estrogen hormone in the body due to ovariectomy and menopause, it affect on immune response (IgG). The purpose of this study was to determine the effect of black soybean extract on immune response (IgG) of implantation mice post ovariectomy. Twenty eight mice were grouped into 4 groups, group 1 (negative control), group 2 (positive control with the administration estradiol concentration 50 ppm), group 3 and 4 were treatment group with the administration of black soybean flour extract doses of 0.31 g/ml and 0.63 g/ml. The data were analyzed using One Way Anova and Duncan Multiple Range Test (DMRT) (p<0,05). The treatment with administration of black soybean flour extract for 10 days in implantation mice post ovariectomy showed that a dose of 0.31 g/ml and dose of 0.63 g/ml had an effect on increasing significantly the immune response (IgG), i.e 0,12987and 0,12996 respectively.

Pengaruh Paparan Asap Rokok Elektrik terhadap Histologi Paru Mencit (Mus musculus L.) Strain Balb/c Jantan Eva Tyas Utami; Bella Dwi Arifianti; Mahriani; Susantin Fajariyah
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Electric cigarette is a type of cigarette that change the liquid containing nicotine and other ingredients in the form of steam. This research aimed to identify the effect of exposure to e-cigarette smoke towards the thickness of bronchioles mucosa and the alveolar septa on male Balb'C mice (M. musculus). Methods: This research was an experimental design used 24 male divided into four groups, i.e., control dan three treatment groups. Treatment groups were given different volume of e-cigarettes liquid, i.e., 1 ml; 2 ml; and 4 ml. The exposure of e-cigarette was done in 4 weeks, respectively. Results: Electric cigarette exposure with different fluid volumes leads to an increase in the bronchioles mucosa's thickness and the alveoli septa. Increasing the volume of fluid used results in an increase in bronchioles mucosal thickness, but decreases the alveoli septa's thickness, resulting in destruction and dilatation of the alveoli. Conclusion: Exposure to e-cigarette smoke with a liquid volume of 1 ml affects the increase in the average thickness of the bronchial mucosa and alveoli septa. An increase in the average thickness of the bronchioles mucosa occurs along with increased fluid volume in exposure to e-cigarette smoke.

Cocoa extract has activity on selectively killing of breast cancer cells line ariza budi tunjung-sari; Mahriani Mahriani; Gusti Agung Perias Tiningrum; Teguh Wahyudi; Misnawi Jati
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.04

Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 Î¼g/ml. The positive control was doxorubicin (0.5-16 Î¼g/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37Â°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 Î¼g/ml (MCF-7) and 1893 Î¼g/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was0,777 Î¼g/ml (MCF-7) and 0,082 Î¼g/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 Î¼g/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 Î¼g/ml, however, 3-fold content resulted in slightly higher LC50 at 780 Î¼g/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property .

EFFECT OF PERIWINKLE (CATHARANTHUS ROSEUS) LEAF EXTRACT ON LIVER HISTOLOGY OF MICE (MUS MUSCULUS L) AFTER ASPARTAME INDUCE Mahriani Mahriani; Rosita Dewi Wulandari; Susantin Fajariyah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 8 No. 2 (2022): February 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.5161

Aspartame is an artificial sweetener in foods and beverages. Continuous use of aspartame will result in oxidative stress in the liver. So we need antioxidants from outside the body such as periwinkle leaves.This study aims to determine the effect of periwinkle leaves (Catharanthus roseus L) on aspartame-induced liver histology. This study used 24 male mice divided into 4 groups, namely negative control (K-) without treatment; Positive control (K +) treatment of aspartame; D1: treatment of aspartame and periwinkle extract doses of 0.42 mg/kg. D2: aspartame and periwinkleÂ extract doses of 0.84 mg/kgbb. Administration of aspartame dose of 0.2 mg /kgbb by intraperitoneal Â for 14 days followed by gavage administration of periwinkle leaf extract for 12 days. The liver was taken on the 27th day by surgery, then made slide histology with Hematoxylin-Eosin staining. The results show that the administration of Catharanthus roseus leaf extract at a dose of 0.84 mg/kgbw in aspartame-induced mice was effective in reducing hepatocyte damage, namely hydrophilic degeneration and necrosis.

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search