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Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 5 No 1 (2016)" : 5 Documents clear
KELIMPAHAN POPULASI DAN PERSENTASE SERANGAN LALAT BUAH YANG MENYERANG TANAMAN BUAH-BUAHAN DI BALI Ni Kadek Nita Karlina Astriyani; I Wayan Supartha; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The study was conducted at the Field and in the Laboratory . Laboratory research was conducted at the Laboratory Integrated Pest and Disease Control Management, Faculty of Agriculture, Udayana University, Bali with altivial 30 meters above sea level from January to March 2014. In field, study was conducted by sampling the fruit flies attacking fruit with Purposive random sampling.The results showed that there were 6 species found in the market and fruits’ center in Bali, namely Bactrocera papayae Drew & Hancock, Bactrocera carambolae Drew & Hancock,Bactrocera umbrosa Fabricius, Bactrocera cucurbitae Coquillete, and Bactrocera albistrigatade Maijere (Diptera: Tephritidae). The spesies fruit flies attack in mango, orange, long chili, rawit chili, watermelon, cucumber, star fruit, sapodilla, nephelium, jackfruit, rose-apple and guava. The abundance of fruit flies population had a positive relationship with the persentage of fruit flies’ attack.
DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR I Gede Agus Adi Chandra; I Dewa Nyoman Nyana; I G N Alit Susanta Wirya; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.
MANFAAT BEBERAPA JENIS MIKROBA YANG DIISOLASI DARI KAYU LARU (Peltophorum pterocarpum.) DAN MUR SEBAGAI STARTER DALAM PEMBUATAN LARU DAN SOPI DI PULAU TIMOR Regina I. M BanoEt; I Made Sudana; I.G.N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The traditional knowledge of farmers in producing alcohol from palm sap can be used as a basis to produce high-value economic products such as medical alcohol, industrial alcohol and ethanol. Food products and traditional fermented beverage are one of the main sources to obtain the potential microbe for microbial fermentation industry. Information on the wood of laru role as agents of microbial cultures and mur for a starter in the making of laru and sopi has not been much identified.The research was conducted in three stages. The first stage of the research, namely the microbial isolation of seven basic materials of making laru and sopi. The identification of microbes by using the API 20C AUX kits and a selection of microbes which have the ability to perform fermentation. The second stage of the research is the production test and gin laru utilize the selection and identification of microbes of, to find the best treatment combination that produces the highest alcohol content. The third stage of the research is the identification of molecular of microbes of culture results that have the best fermentation activities. The first and the third stages were exploratory studies by using the experimental design in the laboratory. The data were collected by means of direct observation. The theoretical analysis was made to describe the experimental results. The second stage of the testing of making laru and sopi to determine the percentage of alcohol content produced. The second stage was an experimental study, designed in the basic experimental design of RAL. The obtained data were analyzed with analysis of variance (ANOVA) to determine the real level of treatment effects, followed by LSD test to determine the level of significant differences between treatments. Each treatment combination was repeated four times. Two controls were used i.e; nira, palm sap without addition of a starter, and a solution of sugar with addition of the laru wood starter. The study was conducted in Labotory of Microbiology and Analytical Chemistry of The Food and Drug Administration Center for Kupang, East Nusa Tenggara.The experimental results showed that the microbes of laru wood and Mur are from the group of yeast. For the making of effective laru and sopi which to be sources of the microbes is red Mur and white Laru. The microbial species were identified as Saccharomyces cerevisiae. The resulting alcohol content on the microbes fermentor of Saccharomyces cerevisiae in the test of making red laru was 8.43%, white laru was 7.05%, sopi and nira/palm wine were 21.44%, and sopi from the aer sugar solution was 23.20%. Saccharomyces cerevisiae can also be combined with wood of laru as a starter in the making of laru and sopi. The combination of the two was the best treatment, resulted in the highest alcohol content at 8.90% of red laru, white laru at 8.40%, sopi from nira at 23.38%, and sopi from aer sugar solution at 23.91%.
KERAGAMAN Liriomyza spp. (DIPTERA;AGROMYZIDAE) YANG BERASOSIASI DENGAN BERBAGAI JENIS TANAMAN SAYURAN DAN TUMBUHAN LIAR DI TIMOR LESTE Americo Alves Brito; I Wayan Supartha; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Field research was conducted in Distrit Ainaro, Aileu, Bobonaro, Dili and Ermera. Maintenance and identification of Liriomyza and parasitoids was conducted in laboratory of Plant Protection, Department of Plant Protection of Agriculture, Ministry of Agriculture and fisheries Timor Leste. The purpose of the study to determine the diversity and abundance of leaf miner populations of Liriomyza spp. and parasitoids on vegetable crops in Timor Leste. The results showed there were two Liriomyza species, associated with host plants in the lowlands to the highlands of Timor Leste. The Both species are Liriomyza sativae (Blanchard) and Liriomyza sp. (Blanchard) The highest population abundance of Liriomyza sativa on tomatoes, red beans, Cucurbita maxima dan Cucumis sativus. while the highest populations of Liriomyza sp. on petsay crop.
STRATEGI PENGENDALIAN PAPAYA RINGSPOT VIRUS (PRSV) PENYEBAB PENYAKIT BERCAK BERCINCIN PADA PEPAYA I Gede Rai Maya Temaja; I Putu Sudiarta; Ni Nengah Darmiati; Ni Made Puspawati
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

ELISA test results of symptomatic leaves from Tabanan, Bali showed that papaya ringspot virus (PRSV) as causing agents of ring spot disease on papaya in Bali. The objective of the study were to determine the control strategies of ringspot disease on papaya in the field. Control strategies of ringspot disease tested in this study were the use of metallic gray plastic mulch and plant barrier. Research result showed that plant barrier can provide a buffer zone to protect papaya that are planted within the confines of the buffer.

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