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Pengendalian Penyakit Layu Fusarium pada Tanaman Cabai Besar (Capsicum annuum L.) dengan Kompos dan Pupuk Kandang yang dikombinasikan dengan Trichoderma sp. di Rumah Kaca SUTARINI, NI LUH WAHYU; SUMIARTHA, I KETUT; SUNITI, NI WAYAN; SUDIARTA, I PUTU; WIRYA, G.N.ALIT SUSANTA; UTAMA, MADE SUPARTHA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Utilization of Trichoderma sp. combined with compost and manure to controlling Fusarium wilt disease on long chilli (Capsicum annuum L.) in greenhouse This study aims to determine the effectiveness of Trichoderma sp. combined with compost and manure to controlling Fusarium wilt disease in long chilli (Capsicum annuum L.). Laboratory studies conducted at the Laboratory of Plant Pathology Faculty of Agriculture, University of Udayana and field research conducted in the greenhouse in the village Pancasari Sukasada Buleleng Subdistrict. The experimental design used was completely randomized designs (CRD) with five treatments were repeated 5 times, each treatment consisted of 5 polybag. The treatments used in this study are Po: control (soil + F. oxysporum f.sp. capsici treatment); P1: compost + soil + F. oxysporum f.sp. capsici; P2: compost + Trichoderma sp. + soil + F. oxysporum f.sp. capsici; P3: cow manure + Trichoderma sp. +  soil  + F. oxysporum f.sp. capsici; and P4: chicken manure + Trichoderma sp. + soil  + F. oxysporum f.sp. capsici The results showed that treatment of Trichoderma sp. able to inhibit the growth of F. oxysporum f.sp. capsici with a percentage of 86.05% when compared to the control treatment at 7 days after inoculation observation in vitro. Application of Trichoderma sp. in compost and manure (cow manure and chicken) were able to suppress Fusarium wilt disease in the greenhouse with the lowest percentage of disease were in P3 and P4 treatment by 4.0% in the observation of 16 week after planting compared with 48.0% of control. Further, the application of Trichoderma sp. in compost and manure (cow manure and chicken) have yields greater than the control (soil without Trichoderma sp.) Keywords: Trichoderma sp., F. oxysporum f.sp. capsici, long chili (Capsicum annuum L.), compost, cow manure, chicken manure
Pengaruh Serangan Penggerek Batang Padi terhadap Hasil Panen Tanaman Padi (Oryza Sativa L.) Di Subak Cemagi Let, Desa Cemagi, Kecamatan Mengwi, Kabupaten Badung RAMADHAN, MOCH BINTANG; SUDIARTA, I PUTU; WIJAYA, I NYOMAN; SUMIARTHA, I KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Rice stem borer is one of the pests that can cause a decrease in rice yield. In Indonesia there are four types of rice stem borer namely S. incertulas, S. innotata, C. suppressalis and S. inferens. This study aims to determine the effect of rice stem borer attack on rice crop yields. The study was conducted for three months in February to April 2019 located in Subak Cemagi Let, Cemagi Village, Mengwi District, Badung Regency, using a diagonal sampling method, the effect of rice stem borer attack on yield reduction. Observations were made on the percentage of rice stem borer infestation, rice stem borer type, wet weight of rice plants. Observation test shows that rice stem borer attack changes every week and affects the rice crop yields.
PERANAN PESTISIDA BOTANI DALAM MENDUKUNG PERTANIAN ORGANIK Sudiarta, I Putu
AGRICA Vol. 3 No. 1 (2010): June
Publisher : Agriculture Faculty of Flores University

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Until recently the use of synthetic pesticides to control pest and plant diseases proved effective, but on the other hand, the excessive use of synthetic pesticides cause many negative effects, such as the development of pest and disease resistance, second pest explosion, death of natural enemies and pesticide residues in food and environment. One alternative that can be done to solve this problem is the use of botanical pesticides. Botanical pesticides are compounds produced as a plant defence response to disturbances and stimulation. These compounds generally are of secondary metabolites that have many functions, such as growth hormones (Auxin, gibberellins and cytokinins), anti-fungal or anti-bacterial, antibiotics, and toxic to animals and insects. The advantage of botanical pesticides is that they have toxicity similar to synthetic pesticides, but the botanical pesticides can be used to support sustainable organic agriculture.  Experiments in the utilization of botanical pesticides to control plant pest have been carried out. One example application is the use of botanical insecticides from ?Brotowali? leaf extract (Tinospora crispa) to control the diamond black caterpillars (Plutella xylostella)  on cabbage plants. In addition, the use of ?basil? oil (Ocimum tenuiflorum) has a real impact in population control of the fruit fly (Bactrocera dorsalis). In addition to pest control, botanical pesticides are also reported to effectively control plant diseases. Use of ?galangal? (Alpinia galanga) and papaya (Carica papaya) has a high ability to inhibit the growth of Ceratocystis sp. On PDA and fruits. The combination of ?betel? leaf extract (Piper betle) and ?galangal? (A galanga) can suppress the growth of banan wilt disease caused by Fusarium oxysporum and / or the Ralstonia solanacearum. The use of ?galangal? (A. Galanga) extract with a concentration of 5%, can also inhibit the growth of stem rot disease (F. oxisporum) on vanilla seedlings. In addition, the use of ?betel? ieaf P. Bettle in the field can suppress black rot disease on cocoa pods (cocoa black pd disease)
THE EFFECT OF TRANSFORMATIONAL LEADERSHIP, WORK ENVIRONMENT AND ORGANIZATION COMMITMENT TOWARD JOB SATISFACTION TO INCREASE EMPLOYEES’ PERFORMANCE Putu Sudiarta
Jurnal Ekonomi & Bisnis JAGADITHA Vol. 5 No. 1 (2018)
Publisher : Magister Manajemen, Program Pascasarjana, Universitas Warmadewa

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The Objective of this study is to identify the effect of transformational leadership, work environment and organizational commitment toward job satisfaction and employees’ performance administration of Warmadewa University, Denpasar Bali. The research approach used was deductive quantitative approach focusing on formal and substantive theory. Hypotheses were formulated to be tested so as to empirical social reality about the employees’ performance. Data collection methods used were questionnaires distributed using Likert scale at the rating interval between 1-5 scores. The sample of the research was 62 administration employees of Warmadewa University of Denpasar Bali as respondents involved in this research. The method of analysis used to determine the relationship between variables was structural model using the method PLS (Partial Least Square) with the aid of SmartPLS 2.0 software. Key research findings included: 1. Transformational leadership has a significant negative impact on job satisfaction, 2. Work environment has an important impact on job satisfaction, 3. Organizational commitment has an important impact on job satisfaction, 4. Transformational leadership has no significant impact on performance employees, 5. Work environment does not have an important impact on employees’ performance, 6. Organizational commitment has an important impact on employee performance, 7. Job satisfaction has an important impact on employees’ performance. Discussions on the results and research findings provided further with addressable suggestions, such as the importance of paying attention to transformational leadership patterns to align in encouraging job satisfaction and employees’ performance.
UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.) Ni Made Dwi Desiyanti; I Made Dira Swantara; I Putu Sudiarta
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.
MOLECULAR IDENTIFICATION OF FUNGI THE CAUSAL AGENT OF STRAWBERRY WILT DISEASE IN BALI Gusti Ngurah Alit Susanta Wirya; I Wayan Diksa Gargita; I Putu Sudiarta
International Journal of Biosciences and Biotechnology Vol 7 No 2 (2020)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The development of strawberry farming in Bali experiencing some obstacles that cause a decline in production, such as wilting disease. The disease was reported caused by the fungi base on morphological recognition. There are two fungi were recognized caused the strawberry wilt disease in Bali, they are from genus Verticillium and Fusarium. More specific information about causal agent of wilt disease in strawberry especially in Bali is needed. The one accurate identification is done through the molecular approach by analyzing DNA that encode the ribosomal DNA (rDNA). The 18S rDNA, including the internal areas of transcribed spacers (ITS), ITS1 and ITS4 have been widely used in phylogenetic studies. The amplification results of this area produce bands in different sizes that can be used to identify fungal species. Based on that the identification of strawberry wilt disease using molecular analysis was conducted. The 542 bp of Internal Transcribed Spacer (ITS) DNA was successfully amplified using PCR with pairing primers ITS 1 (5-TCCGTAGGTGAACCTGCGG-3’), and ITS 4 (5’-TCCTCCGCTTATTGATATGC-3’). The sequences of three isolates were successfully obtained through sequencing. Homology levels were tested between sequences and showed that Candi Kuning sequence and Gobleg sequence had 95% similarity with sequence of Fusarium oxysporum NRRL 13307 (U34571) from America. While Pancasari sequence have 94% similarity with sequence of Fusarium oxysporum NRRL 13307 (U34571) from America. Candi Kuning, Gobleg, and Pancasari sequences had the same 86% with sequence of Fusarium oxysporum isolate C34-294 Brazil (KJ439088) and had 89% similarity with sequence Fusarium oxysporum f.sp. fragariae China (KT833080). Homology levels were tested between sequences and showed that Candi Kuning sequence and Gobleg sequence had 95% similarity with sequence of Fusarium oxysporum NRRL 13307 (U34571) from America. While Pancasari sequence have 94% similarity with sequence of Fusarium oxysporum NRRL 13307 (U34571) from America. Candi Kuning, Gobleg, and Pancasari sequences had the same 86% with sequence of Fusarium oxysporum isolate C34-294 Brazil (KJ439088) and had 89% similarity with sequence Fusarium oxysporum f.sp. fragariae China (KT833080). Based on phylogeny analysis of Pancasari, Gobleg and Candi Kuning isolates were obtained in one group with Fusarium oxysporum identified in America and Brazil, and also in one group with Fusarium oxysporum f. sp. fragariae that identified in China.
MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE I Putu Sudiarta; Dwi Martiningsia; I Nyoman Wijaya
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.
IDENTIFICATION OF CITRUS WHITEFLY, HOST OF ENTOMOPATHOGENIC FUNGI (ASCHERSONIA PLACENTA) IN BALI INDONESIA Ni Putu Merthaningsih; I Putu Sudiarta; Gusti Ngurah Alit Susanta Wirya
International Journal of Biosciences and Biotechnology Vol 7 No 2 (2020)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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One of the pests of citrus is whitefly that, causes damage directly or/and indirectly to the citrus production. To control whitefly the farmer usually use chemical insecticide, however the utilization of chemical insecticide has been reported to haves many negative effect. To minimize the utilization of chemical insecticide, the environmentally friendly method is needed. One of the method is to utilize the natural enemies. Natural enemies are including, parasitiod, predator as well as insect pathogen (entomopathogen). In 2017 entomopathogenic fungi Aschersonia placenta was found to be associated with citrus whitefly in Bali Indonesia. However the species of whitefly has not been identified. In this research the identification of whitefly, the host insect of A. placenta was conducted based on morphological and molecular identification. Morphological identification of whitefly use puparial stage, started with sample preparation by Slide Mounting Protocol. The target of mitochondrial cytochrome c oxidase subunit I (mtCOI) gen was successfully amplified (700 bp) by PCR using forward primer LCO 5'GGTCAACAAATCATAAAGATATTGG3' and reverse primer HCO 5'TAAACTTCAGGGTGACCAAAAAATCA3'. The phylogenetic analysis using software ChromasPRO, Molecular Evolutionary Genetics Analysis (MEGA 5.05), PAUP, BioEdit, and TreeGraph2 was conducted. The result shows that the mtCOI sequence of P. minei from Bali (LC491421) has the highest percentage among others with MK421974 P. minei (score homology 96%). The morphological recognition and sequence analysis show that the species of citrus whitefly is Paraleyrodes minei.
SOSIALISASI DAN PENERAPAN TEKNOLOGI PEMELIHARAAN PARASITOID TELUR SEBAGAI AGEN HAYATI PENGENDALI HAMA PENGGEREK BATANG PADI KETUT SUMIARTHA; I PUTU SUDIARTA
Buletin Udayana Mengabdi Vol 6 No 1 (2007): Volume 6 No.1 - April 2007
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT This socialization and application conservation technology of egg parasitoid for biological control of the rice steam borer was conducted in order to give information for the farmer and application method of conservation technology egg parasitoid for biological control of the rice steam borer. It was conducted at August 14, 2006 at the subak building and agriculture area of Subak Lungatad, Banjar Jenah, East Peguyangan Village, West Denpasar District, Denpasar City, and Bali Province. Key Note Speakers come from Department of Plant Protection, Faculty of Agriculture, Udayana University (: Ir. I Ketut Sumiartha, MA.gr., I Putu Sudiarta, SP.MSi., Ir. I Wayan Susila, MS., Dr. G. N. Alit Susanta Wirya, SP., MA.gr., and Ir. I Gusti Ngurah Bagus, MP.), and also comes from Department of Agriculture Office Denpasar (I Wayan Swastika, SP., MSi.).The farmers like to follow the program and have understood about rice steam borer, egg parasitoids and applied method conservation technology of egg parasitoid. They hope those information can be accepted sustainable.
TEKNIK GRAFTING DAN BUDIDAYA HORTIKULTURA PADA KELOMPOK TANI MODEL KAWASAN RUMAH PANGAN LESTARI DI DESA GETASAN- KABUPATEN BADUNG I N. Suarsana; I. W. Suardana; I K.M. Budiasa; I P. Sudiarta; I G. Wijana
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Guna mengurangi penyakit-penyakit pada tanaman tomat maka perlu dilakukan penyambungan dengan tanaman terong sehingga hortikultura tersebut tahan terhadap penyakit. Tujuan kegiatan ini adalah meningkatkan pengatahuan grafting (penyambungan) dan budidaya hortikultura dengan sistim polibag. Metode yang digunakan adalah pendekatan dengan advokasi (penyuluhan) dan metode edukasi (pelatihan) grafting (teknik penyambungan). Hasil kegiatan menunjukkan bahwa kelompok tani Model Kawasan Rumah Pangan Lestari (MKRPL) mampu melakukan teknik budidaya hortikultura mulai pemilihan bibit dan persyaratanya, syarat pertumbuhan, teknik penyediaan media tanam (tanah dan pupuk), penyemaian bibit, dan cara bertanam dengan sistim polibag. Kelompok MKRPL mampu menguasai teknik grafting (penyambungan) dan melakukan penyambungan tanaman terong pada bagian bawah dan tanaman tomat pada bagian atas dengan hasil baik. Dapat disimpulkan bahwa kelompok tani Model Kawasan Rumah Pangan Lestari (MKRPL) mampu melakukan grafting (penyambungan) dan budidaya hortikultura dengan sistim polibag dan dapat memanfaatkan lahan kosong untuk diisi dengan tanaman hortikultura sehingga lebih produktif.
Co-Authors - Hamid Agung, I Dewa Agung Putra AGUS PUTRA SEDANA Ali Nurmasyah Americo Alves Brito Anak Agung Ayu Agung Sri Sunari Anak Agung Sagung Kendran ANANDA RIZKI NURANI ARIYANTA, I PUTU BAWA Chiharu Hongo Damastra, Garda Bagus Desak Putu Sudarmini Devi, Komang Saraswati Devi, Putu Shinta DEWA AYU DIAH PUSPITASARI Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DINA RAHMAWATI Dinarkaya, Shah Mahapati Dwi Martiningsia Dwi Martiningsia DWI SUGIARTA DWI WIDANINGSIH Eka Wijayanti, Febri G.A.M.K. Dewi GARGITA, I WAYAN DIKSA Gede Suastika Gede Suastika GREG LUTHER GREGORY C. LUTHER Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Alit Susanta Wirya GUSTI NGURAH GEDE DHARMA PUTRA PUTRA Hamid, - Harianja, Shara Yulita Hartha , I Komang Gede Suweca I DEWA MADE PUTRA WIRATAMA I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I G. Wijana I GEDE PUTU WIRAWAN I GEDE SILA ADNYANA I Gst Agung Putu Raka Agung I GUSTI AYU DEVI VALENIA SARI I Gusti Ngurah Bagus I Gusti Ngurah Kade Mahardika I Ketut Mangku Budiasa I KETUT PURNA YASA I KETUT SUMIARTHA I Komang Candra Giri Prayoga I KOMANG VIDIA DHARMA TARO PUTRA I Made Arimbawa I Made Dira Swantara I Made Sudana I MADE SUDARMA I Made Sukewijaya I MADE SUPARTHA UTAMA I MADE WINANTARA I MADE YESTA SANTIATMA I NYOMAN DARMA YASA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I PUTU DHARMA I PUTU WIRYA SUPUTRA I Wayan Arta Wijaya I WAYAN RUSMAN I Wayan Suardana I Wayan Supartha I Wayan Susila I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari IDA BAGUS GDE PRANATAYANA Ida Bagus Kade Suardana JOKO MARIYONO JOKO MARIYONO KETUT AYU YULIADHI Klett, Katrina Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi Made Mika Mega Astuthi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga MARIA ULFA ELLA ELLA Masahiro Shishido MOCH BINTANG RAMADHAN NI KADEK NINA ARI SUCI Ni Kadek Nita Karlina Astriyani Ni Luh Gede Sudaryati NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE DWI ANTARI Ni Made Dwi Desiyanti Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE WINDA UTARI NI NENGAH DARMIATI Ni Putu Merthaningsih Ni Putu Noviyanti Ni Wayan Korniasih NI WAYAN SUNITI NYOMAN SEMADI ANTARA Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna R. SRINIVASAN RAMADHAN, MOCH BINTANG S.K. Widyastuti SANJAYA, I GUSTI NGURAH PRABU WIRA Selangga, Dewa Gede Wiryangga SONIA ASHA HASARI Sri Kayati Widyastuti SUGIARTA, DWI Sumartayasa, I Wayan Andi SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Yuliadhi , Ketut Ayu Zhao, Tiejun