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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 9 Documents

Search results for , issue " Vol 10, No 1 (2010)" : 9 Documents clear

STRUKTUR DAN KOMPOSISI JENIS TUMBUHAN HERBA DAN SEMAI PADA HABITAT SATWA HERBIVOR DISUAKA MARGA S ATWA CIKEPUH,SUKABUMI, JAWABARAT [Structure and Composition of Herbaceous and Seedling Communities on the Herbivore Habitat within Cikepuh Wildlife Sanctuary, Sukabumi, West Java] Sadili, Asep
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research was conducted to study the structure and composition of herbaceous and seedling communities (understorey species)occurred on herbivore habitat within Cikepuh wildlife sanctuary. A series of 25 plots of 1 m x 1 m each with an interval of 5 m were used to establish a species-area relationship. The proportion (percentage) of canopy (leaf) coverage of each species occurred within the sampling plots to the sampled soil surface area was measured. A total of 54 species belonging to 47 genera and 29 families occurred in the 25 plots develoved, with the species diversity index (value) of 2.09 using the Shannon Wiener formula. Nine species were categorized as herbaceous plants (16.67 %) while the other 45 species were categorized as seedlings (83.33 %). The average density measured was 39 individuals/m or c. 386.400 individuals/ha. The most dominant family is Euphorbiaceae (possessing 5 diffrent species) while the most dominant species is Urochloa subquadripara (Poaceae) with and importance value of 96.08%.

PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.

PENGETAHUAN LOKAL DAN PEMANFAATAN TUMBUHAN OLEH MASYARAKAT LOKAL PULAU KABAENA- SULAWESI TENGGARA [Local knowledge and Plant Utilization by Local People of Kabaena Island Southeast Celebes] Rahayu, Mulyati; Rugayah, Rugayah
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

An ethnobotanical research focusing on the local people Moronene of the Wumbu Buro village, eastern part of Kabaena island,South East Sulawesi, was carried out using semi structural interviews and field observation. The local people practiced a traditional agricultural system. The results indicated maize Zea mays L., rice Oryza sativa L. and some other vegetable plants becoming the first priority species planted before cultivating horticultural plants such as cashew Anacardium occidentale L., cacao Theobroma cacao L. and coconut Cocos nucifera L. A total of 65 plant species were used by the local people for their daily needs, such as for food, utensil, roof, waving, medicine & cosmetic purposes including a plant species used for harvesting the palm sugar.The indigenous knowledge owned by the local people in utilizing plant species is discussed.

PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl [The Change of Serum Cholesterol Level in Rats after Consuming Maltooligosaccharide Synthesized by Enzimatic Reaction of Bacillus licheniformis BLl Amylase] Dinoto, Achmad; Rahayu, Rita Dwi; Satyaningtijas, Aryani S
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.

KAJIAN SUPERPARASIT DAN PREFERENSI INANG BENALU Viscum articulation Burm. f. (Viscaceae) DIKEBUN RAYA PURWODADI DAN CIBODAS [Study on superparasite and host preference of the the mistletoe Viscum articulatum Burm. f. (Viscaceae) in Purwodadi and Cibodas Botanic Gardens, Java] Sunaryo, Sunaryo
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Superparasite is a parasitic plant that lives on other parasitic plant. Viscum articulatum Burm. f. (Viscaceae) is a parasitic species of mistletoe that grows on other parasitic species of mistletoes, thus a superparasite. In Purwodadi Botanic Gardens-LIPI, East Java V. articulatum grows on Dendrophthoe pentandra and Macrosolen tetragonus. In Cibodas Botanic Gardens-LIPI, West Java it grows on D. pentandra and M. cochinchinensis. Dendrophthoe pentandra is the most infected species.The infected mistletoes are regarded as the first stage hosts.The living collections at both Botanic Gardens parasited by the three mistletoes are regarded as the second stages host.Investation preference for first and second host stages is studied in this research.

KERAGAMAN GENETIK, HERTTABILITAS DAN KORELASI BEBERAPA KARAKTER AGRONOMI PADA GALUR F2 HASIL PERSILANGAN KACANG HIJAU (Vigna radiata (L.) Wilczek) [Genetic Variability, Heritability and Correlation of some Agronomic Characters in the F2 of Varietal crosses of Mungbean {Vigna radiata (L.) Wilczek)] Hakim, Lukman
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The F2 progenies of two crosses among three mungbean varieties were evaluated with their parents (Varsha, Park it and Local Belu)at Muara Experimental Station, Bogor during dry season of 2007. The experiment was conducted in a randomized complete block design with three replications. The seed of each F2 progenies and parents were planted in four rows of four meters long.Plant spacing was 40 x 20 cm, each will contained one plant. Among the character studies, days to maturity, seed yield per plant and plant height had the highest coefficient of variability, with the mean of 51.3%, 49.8%, and 49.2% respectively.Number of branches, pod length and number of seed per pod had the lowest coefficient of variability, and the mean were 19.1%, 18.8%, and 17.6%.The heritability estimates of nine agronomic characters studied ranged from IS.6 to 65.2%. Plant height and number of pods per planthad the highest heritability estimates with mean of 65.2% and 58.6% respectively for the two crosses. The mean heritabilityestimate for seeds yield per plant, pod length and number of seeds per pod were the lowest of 19.4%, 18.3% and 15.6% respectively. Based on the F2 data, selection of plant height and days to maturity had the highest expected genetic advance of 45.8 and 41.0%.The mean expected genetic advance for pod length and number of seeds per pod were the lowest of 19.4 and 17.4%.Correlation coefficient between grain yield with plant height, number of pods per plant and seed size were positively significant (r = 0.404, 0.613 and 0.422 respectively). Correlation coefficient between grain yield with other agronomic characters were small or negative. Therefore, plant height, number of pods per plant and seed size can be used as the selection criteria in selecting mungbean genotypes for high yield.

KAJIAN ETNOBOTANI PANDAN SAMAK (Pandanus tectorius Sol.) DIKABUPATEN TASIKMALAYA, JAWA BARAT Susiarti, Siti; Rahayu, Mulyati
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2058

Members of the genus Pandanus (Pandanaceae) are used by Indonesian people as food, medicinal and handycraft materials.Three species of Pandanus, namely pandan wangi (P. amaryllifolius), cangkuang (P. furcatus) and pandan samak (P. tectorius) are known in Tasikmalaya, West Java. Pandan samak which has several different local names is the cultivated ones.This species is well known as handycraft from Tasikmalaya and had been exported to other countries. Incomes from the handycraft products is the second biggest source after agricultural sector.

PENGARUH RADIASI DAN LOKASI TUMBUH TERHADAP PERTUMBUHAN DAN PENYAKIT HAWAR DAUN TALAS kv KETAN Sukamto, L Agus; Saefudin, Saefudin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Taro (Colocasia esculenta (L.) Schott) is an important tuber crop, grown widely in humid tropics and a source of carbohydrate for many people in Asia, Africa, and the Pacific islands. The leaves and stalks of taro are used as vegetable, as they contain a high protein that is useful for people diet in developing countries. Taro is underutilized plant that has potency as an alternative food.Plant propagation of taro is usually done by using its rhizome or stolon so that the genetic diversity is very limited. In vitro and irradiated techniques can increase its genetic diversity and multiply the plant that has superior quality and quantity.Taro "Ketan" grown tissue culture have been irradiated with gamma rays S Gy and 10 Gy were planted on two different environmental locations.There were many variants as the results of these treatments. Gamma rays and environmental locations caused genotype changes that were stolon number, plant height, corm fresh weight, and leaf blight disease.Gamma rays caused morphological changes that were orientation, undulation, outline of sinus, margin of lamina,and petiole color.

AKTIVITAS ANTIOKSIDAN DAN ANALISIS KIMIA EKSTRAK DAUN JUNGRAHAB (Baeckeafrutescens L.) Murningsih, Tri
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Extraction of Jungrahab (Baeckea frutescens L.) leaves was done by using organic solvent (ether). The antioxidant activity of the extract was investigated using l,l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Ascorbic acid (vitamin C) was employed as a positif control. By virtue of their hydrogen donating ability, the extract and ascorbic acid exhibited reducing power.The extract was found has activity in free radical scavenging activity against DPPH. The antioxidant activity was supposed to be due to the composition of certain constituens e.g. 2,6-di(/-buthyl)-4-hidroxybenzoie acid (BHT-acid), 5,7-dihydroxy-6-methylflavanone and a-tocopherol (vitamin E) within the extract.

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