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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 14 Documents
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Search results for , issue " Vol 4, No 5 (1999)" : 14 Documents clear
REGENERASI TANAMAN DARI JARINGAN KOTILEDON +RUAS BENIH KEDELAI, {Glycine max L., KULTIVAR WILIS) DENGAN RADIASI SINAR GAMMA [Plant Regeneration from Cotyledons of Mature Soybean ( Glycine max L. Wilis Cultivar Using Gamma Rays] Hutabarat, Dameria; Ratma, Rivaie
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.161 KB) | DOI: 10.14203/beritabiologi.v4i5.1245

Abstract

Soybean Wilis cultivar was efficiently regenerated in vitro via somatic embryogenesis. Cotyledonary explants were excised from mature geminating seeds. Seeds were germinated on agar solution and on B5 medium enriched with 5 ppm BA, 0,25 pom IBA and 500 ppm casein hydrolyzate. Cotyledonary nodes from both germinating seeds were excised and cultured on B5 medium enriched with 5 ppm BA, 0,25 ppm IBA and 500 ppm casein hydrolyzate. Age of seedlings had a remarkable influence on shoot regeneration. Cotyledon from seeds germinated on agar solution with light gave better result in shoot regeneration compare with those germinated in darkness. The highest number of regenerants per explants (5 shoots) was produced by cotyledon from seeds germinated on 65 medium enriched with 5 PPM BA, 0,25 PPM IBA and 500 PPM casein hydrolyzate in darkness. The seeds of soybean were exposed to gamma-rays doses 10 Gy then germinated on S5 medium enriched with 5 PPM BA,0,25 PPM IBA and 500 ppmcase/n hydrolyzate did not improve the number of plant regeneration.Only 5-day-old seedlings from seeds were exposed to gamma-rays dose 30 Gy could improve the number of shoot regeneration, one of the cotyledonary node treated produced 21 regeneration shoots.
PENGUJIAN PERTUMBUHAN DAN DAYA HASIL BEBERAPA GALUR TANAMAN CABE MERAH {Capsicum annuunft.) HASIL PERLAKUAN COLCHICINE [Growth and Yield Potential Testing of Some Colchicine-treated Red Pepper (Capsicum annuumh.)Lines}. Witono, Joko R
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.721 KB) | DOI: 10.14203/beritabiologi.v4i5.1250

Abstract

This experiment aims to examine first generation (F1) of some lines of red pepper (taosicum annuum L) resulted by colchicine treatments,the examinatton covering growth and yield characters.The method was Randomized Complete Block Design (RCBD) with 16 lines resulted generation combination soaking time in colchicine solution (0, A 16, and 24 hours) and the red pepper varieties (Cipanas, Bengkulu, Jatilaba, and TIT super). The best growth character is 65, and the best yield character isG16.High heritability characters (height of plant, age of blossom, length of fruit, diameter of fruit, and weight of fruit) could be chosen for selection and plant breeding programs.
ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) [Genetic Variation Analysis of Ethanol Tolerance Yeast, Saccharomyces cerevisiae Dl by Using RAPD] Julistiono, Heddy; Yulineri, Titin; Hanjono, Sukamto
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.724 KB) | DOI: 10.14203/beritabiologi.v4i5.1240

Abstract

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.461 KB) | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.

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