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All Journal Jurnal Teknologi Dan Industri Pangan bionature AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Indonesian Community Journal
Joko Sulistyo
Universitas Ciputra Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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BIOPROSES PENGURAIAN LIMBAH CAIR BUANGAN INDUSTRI PANGAN DAN NON-PANGAN BERBASIS BIOSURFAKTAN Yati Sudaryati Soeka; Joko Sulistyo
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.35848

Abstract

Biosurfactants are microbial metabolites that may be utilized to replace synthetic chemical surfactant in overcoming the problem of industrial wastewater pollution. Indications of the presence of biosurfactant activity may be determined through measurement of emulsification and enzymatic activity of the tested microorganisms on their growth media, which is indicated by decreasing the BOD and COD of the tested growth media containing wastewater material as an inducer added to the microbial growth medium. This research was conducted to examine several potential microbial isolates that produce biosurfactants on growth media containing mixed waste water. The results showed that some of the microbial cultures tested showed the potential to produce biosurfactants as indicated by the emulsification and enzymatic activity of the tested microbial culture. Trial on mixed industrial wastewater showed that the tested biosurfactants were able to reduce the levels of BOD (440-1300 mg/l) and COD (1240-1760 mg/l) of the wastewater samples up to low BOD levels (82 mg/l) and COD (116 mg/l), respectively, thus that it meets the requirements of the established wastewater quality standards according to the Indonesian Standard regarding with the respective BOD (50-100 mg/l, 06-6989.9-2004 SNI) and COD (100-250 mg /l, 06-6989.2-2004 SNI).Key words : biosurfactant, emulsification, microbial enzyme, wastewater, BOD, COD.
Application of Bioprocessed SBE Based Biovigorant as Soil Enhancer Joko Sulistyo; Bambang Gunawan
AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian Vol 9 No 2 (2022)
Publisher : UNIVED Press, Dehasen University Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37676/agritepa.v9i2.2855

Abstract

One of efforts to accelerate the process of restoring degraded agricultural lands is to use soil enhancer or soil conditioner in order to create an environment that is conducive to environmental protection, support plant growth and production, development of soil biota, and increase soil resistance to erosion, thus the soil enhancer must have the ability to facilitate optimal supply of nutrients, water and air. This study used a completely randomized design consisting of several treatments, respectively; Control (no soil enhancer), Zeolite (inorganic soil enhancer), and Biovigorant based bioprocessed spent bleaching earth (SBE, organic soil enhancer). The results showed that the biovigorant met the requirements of environmental quality standards based on government regulation and fulfills the technical quality requirements as a soil enhancer with special functions. Although the use of biovigorant may improve the soil chemical properties, however, it does not have a significant impact on agronomic factors. The use of biovigorant did not accumulate heavy metals, such as AS, Hg, Pb, Cd, Cr, Ni towards soil, water, and plants, but has a comparable effect with zeolite regarding with chemical properties and plant growth. As an organic soil enhancer, biovigorant meets all the minimum technical requirements. The use of biovigorant in agricultural crops, especially maize, did not contribute negatively to the accumulation of the heavy metals in the surrounding soil and water, and furthermore, based on mass balance analysis, the biovigorant did not provide the potential for accumulation in the next growing season.
Preparation of Active Food Packaging and Coating Material Based on Bacterial Cellulose to Increase Food Safety Joko Sulistyo; Prasetyon Sepsi Winarno; Ika Yohanna Pratiwi; Lorent Permata Ridfan; Katherine Mahadewi Pranata; Raja Munirah Raja Chick
Jurnal Teknologi dan Industri Pangan Vol. 34 No. 1 (2023): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2023.34.1.48

Abstract

The use of bacterial probiotic metabolite-based active-packaging and coatings is an innovative approach that has gained widespread attention worldwide. Additionally, its utilization can lead to improvements in qualities and properties of food products. This study was aimed to develop a food spoilage prevention system using active food packaging and coating material in preventing food spoilage while increasing its shelflife. The materials used were bacterial cellulose (BC) based bioplastics fortified with fermented soymilk extracts (FSME) using Lactobacillus acidophilus as the producer of the antimicrobial and antioxidant agents. Moreover, the applications of FSME containing probiotic bacterial metabolites are discussed to highlight their efficacy in enhancing the quality and shelf life of food products.The antimicrobial test showed that the FSME could inhibit the growth of pathogenic microbial cultures at minimum inhibitory concentration (MIC) of 10% (v/v) as shown by clear zones, around colonies of E. coli (14.33±0.58 mm), S. aureus (18.33±6.03 mm), S. Typhimurium (11.67±1.15 mm), L. monocytogenes (11.33±2.31 mm), and B. cereus (13.33±3.06 mm). Meanwhile the results of IC50 for antioxidant activity test (µg/mL) indicated that the FSME showed radical scavenging activity against DPPH at approximately 75.27±2.552 (2.5%, v/v), 55.00±0.791 (5.0%, v/v), 43.17±1.603 (7.5%, v/v) and 15.05±0.346 (10%, v/v), respectively. The shelflife of strawberries coated with the active food coating using the bioplastic fortified with FSME showed an increase in shelf life of 14 days at 4°C. The overall results indicated that the use of BC based bioplastics fortified with FSME can play an important role in preventing premature spoilage and increasing the shelf life of food products.
Some Properties of Oyster Mushroom Grown on Substrates Containing Paper Waste Joko Sulistyo
bionature Vol 24, No 2 (2023): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i2.45548

Abstract

ABSTRACTThis study was conducted to determine the activity of starter culture of oyster mushroom and influence of substrates containing paper waste materials used to grow the mushroom. The purpose of the study was to analyze nutritional value and antioxidant activity of the mushroom grown on the substrate contained paper waste. The substrate was prepared using paper waste with 5 different formulations were 0% (control), 20%, 30%, 40%and 50% paper waste. Proximate analysis, heavy metal content and antioxidant activity were carried out to test the quality of the mushroom. Overall, the mushroom grown on the substrates containing paper waste showed high water content, however, the mushroom grown on the substrate contained 50% paper waste showed the highest content of ash, protein, and crude fiber, while higher fat content showed by the mushroom grown on the substrate contained 50% paper waste compared to the mushroom grown on other substrate with different composition of paper waste. The mushroom grown on the substrate contained 50% paper waste indicated considerably high content of heavy metals such as lead, (3.5%), copper (20.8%) and cadmium (4.4%), respectively however, it showed the higher antioxidant activity (87.19%) as well compared to the mushrooms grown on the substrate containing a lower percentage of paper waste, thus giving a conclusion that there was probably positive correlation in between the heavy metals contained in paper waste with antioxidant activity. Keywords: paper waste, oyster mushroom, proximate analysis, antioxidant, heavy metals. ABSTRAKPenelitian ini dilakukan untuk menentukan aktivitas kultur starter jamur tiram dan menguji substrat yang mengandung limbah kertas yang digunakan untuk menumbuhkan jamur. Tujuan penelitian adalah untuk menganalisis nilai gizi dan aktivitas antioksidan dari jamur yang ditumbuhkan pada substrat mengandung limbah kertas. Substrat disiapkan menggunakan limbah kertas dengan 5 formula berbeda yaitu 0% (control), 20%, 30%, 40% dan 50%. Analisis proksimat, kandungan logam berat dan aktivitas antioksidan dilakukan untuk menguji kualitas dari jamur. Secara keseluruhan, jamur tiram yang ditumbuhkan pada substrat limbah kertas menunjukkan kadar air yang tinggi, namun jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kadar abu, protein, dan serat kasar yang tertinggi, sementara kadar lemak pada jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan lebih tinggi dibandingkan dengan jamur yang ditumbuhkan pada substrat dengan komposisi limbah kertas lainnya. Jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kandungan logam berat, masing-masing seperti timbal (3.5%), tembaga (20.8%) dan kadmium (4.4%) yang cukup tinggi, namun juga menunjukkan aktivitas antioksidan yang lebih tinggi (87.19%) dibandingkan dengan jamur yang ditumbuhkan pada substrat mengandung persentase limbah kertas yang lebih rendah, sehingga memberikan kesimpulan bahwa terdapat korelasi yang positif antara logam berat yang terkandung dalam limbah kertas dengan aktivitas antioksidan.Kata kunci: limbah kertas, jamur tiram, nilai gizi, analisis proksimat. Antioksidan, logam berat.
Biological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko; Joko Sulistyo
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.37 KB) | DOI: 10.14203/jbi.v3i3.3467

Abstract

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.461 KB) | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Soeka, Yati Sudaryati; Naiola, Elidar; Sulistyo, Joko; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.43 KB) | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
DEVELOPMENT OF PURE CULTURE STARTER FOR KECAP, AN INDONESIAN SOY SAUCE [Pengembangan Ragi Kecap Khas Indonesia Dengan Biakan Murni] Joko Sulistyo; Sulistyo, Joko; Nikkuni, Sayuki
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.719 KB) | DOI: 10.14203/beritabiologi.v7i6.863

Abstract

In order to prevent aflatoxin contamination during the production of kecap, an Indonesian soy sauce, a white-spored mutant K-IA strain induced from an aflatoxin-negative koji mold was applied to koji making process of kecap. While kecap koji making process took 9 days by conventional method without inoculum, the use of K-IA strain as a starter shortened the process to 3 days at room temperature. The koji prepared with this starter contained 2.3 x 107 cfu/g of Aspergillus sp. K-IA as the dominant speciesand some contaminants from the environment such as other species of Aspergillus (2.0 x 10 cfu/g) and Mucorales (1.4 x 10s cfu/g). The mash prepared with kecap koji inoculated with this starter contained higher formol nitrogen (FN) and water-soluble nitrogen (WN) than those prepared with koji inoculated without any starter.These results demonstrated that the use of this starter did not only contribute to the prevention of aflatoxin contamination but also improved the conventional kecap fermentation process.
ANALISA ASAM ORGANIK DAN ASAM AMINO PADA NATTO YANG DIFERMENTASI OLEH 7 STRAIN BACILLUS NATTO Joko Sulistyo; SULISTYO, JOKO
BERITA BIOLOGI Vol 3, No 6 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.539 KB) | DOI: 10.14203/beritabiologi.v3i6.1326

Abstract

JOKO SUUSTYO. 1986. Analysis of organic acid and amino acid in natto fermented by 7 strains of Bacillus natto. Berita Biologi 3 (6): 277 - 282. The ability of seven strains of Bacillus natto produced a number of organic and amino acid components in natto were investigated. Raw, submerced and autoclaved soybeans for natto contained 1.15% 0.46% and 0.77% in organic acid and 0.19%, 0.30% and 0.30% in amino acid, while after fermentation those range were 0.36 - 0.90% in organic acid and 2.86 -6.08% in amino add. During submercing, autoclaving and fermenting, citric acid component decreased down very sharply to about one hundredth of starting level. While acetic,iso-butiric and iso-valeric components increased up to 84.92%, 96.60% and 95.87%, respectively. Mean while, glutamic acid, iso-leucine,leucine andphenylalanine components of amino acid increased up to 84.36%, 90.52%, 91.67% and 95.88%, respecti
Pelatihan Pengembangan Inovasi Bioaktivator Untuk Meningkatkan Kualitas Pangan Organik Berbasis Sistem Pertanian Organik Joko Sulistyo; Irra Chrisyanti Dewi; Adrie Oktavio
I-Com: Indonesian Community Journal Vol 4 No 2 (2024): I-Com: Indonesian Community Journal (Juni 2024)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33379/icom.v4i2.4335

Abstract

One effort to produce organic food products is to increase the productivity of organic farming using bioactivators to create a conducive environment to support the growth and fertility of soil microbiota, protect the surrounding environment, and increase soil fertility. Organic food is managed in such a way as to realize sustainable productivity. Therefore, the provision of bioactivators as decomposers of organic matter in the soil can facilitate the maximum presence of nutrients, water and air. Trial of the use of bioactivators consisting of a consortium of microorganisms as agents for decomposing organic matter in soil. The research results show that bioactivators can repair the chemical, physical, and microbiological properties of soil. The use of bioactivators does not cause the heavy metals accumulation in water, soil, and plant growth. As a decomposer of soil organic matter, bioactivators meet the minimum of technical requirements. The use of bioactivators as decomposers of soil organic matter to support organic agricultural commodities so as not to have a negative impact on the accumulation of heavy metals in soil, water and the surrounding environment.
Co-Authors Adrie Oktavio Bambang Gunawan Evi Triana Ika Yohanna Pratiwi Irra Chrisyanti Dewi Joko Sulistyo Katherine Mahadewi Pranata Lorent Permata Ridfan Naiola, Elidar Napitupulu, Rostiati NR Nikkuni, Sayuki Prasetyon Sepsi Winarno Raja Munirah Raja Chick Widiasih, Lutfi Erlita Yati Sudaryati Soeka Yati Sudaryati Soeka