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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 34 Documents
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Search results for , issue "Vol 10, No 1 (2010)" : 34 Documents clear
PENGARUH PEMBERIAN JERAMI PADI TERHADAP PERTUMBUHAN TANAMAN PADI (Oryza sativa) DI TANAH SULFAT MASAM Arifin Fahmi
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2045

Abstract

The objective of the experiment was to investigate the effect of rice straw application on the growth of rice in two conditions of acid sulphate soils. An experiment was conducted in the glass house consisting of three treatment factors using the completely randomized design with three replications.The first factor was soil condition consisting of continuous application of rice straw and without application of rice straw during the last 5 years. The second factor was fresh application of rice straw consisting of application and without application, whereas the third factor was P application consisting of 3 rates of SP - 36 fertilizers.Parameters measured were plant height, tiller number and Fe toxicity score. Measurement were conducted at 2, 4, 6 and 8 weeks after planting. Plant dry weight and P concentration in plant tissue were determined at 8 weeks after planting. The result showed the main effect of rice straw application was significant decrease on the tiller number of the rice plant at 2, 4 and 6 weeks after planting, whereas significant on plant height was observed at 2 and 4 weeks after planting. Based on the scoring of degree of Fe toxicity, it was concluded that the rice plant did not exhibit any effect of Fe toxicity.
PENGARUH RADIASI DAN LOKASI TUMBUH TERHADAP PERTUMBUHAN DAN PENYAKIT HAWAR DAUN TALAS kv KETAN L Agus Sukamto; Saefudin Saefudin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2059

Abstract

Taro (Colocasia esculenta (L.) Schott) is an important tuber crop, grown widely in humid tropics and a source of carbohydrate for many people in Asia, Africa, and the Pacific islands. The leaves and stalks of taro are used as vegetable, as they contain a high protein that is useful for people diet in developing countries. Taro is underutilized plant that has potency as an alternative food.Plant propagation of taro is usually done by using its rhizome or stolon so that the genetic diversity is very limited. In vitro and irradiated techniques can increase its genetic diversity and multiply the plant that has superior quality and quantity.Taro "Ketan" grown tissue culture have been irradiated with gamma rays S Gy and 10 Gy were planted on two different environmental locations.There were many variants as the results of these treatments. Gamma rays and environmental locations caused genotype changes that were stolon number, plant height, corm fresh weight, and leaf blight disease.Gamma rays caused morphological changes that were orientation, undulation, outline of sinus, margin of lamina,and petiole color.
STRUKTUR DAN KOMPOSISI JENIS TUMBUHAN HERBA DAN SEMAI PADA HABITAT SATWA HERBIVOR DISUAKA MARGA S ATWA CIKEPUH,SUKABUMI, JAWABARAT Asep Sadili
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2050

Abstract

The research was conducted to study the structure and composition of herbaceous and seedling communities (understorey species)occurred on herbivore habitat within Cikepuh wildlife sanctuary. A series of 25 plots of 1 m x 1 m each with an interval of 5 m were used to establish a species-area relationship. The proportion (percentage) of canopy (leaf) coverage of each species occurred within the sampling plots to the sampled soil surface area was measured. A total of 54 species belonging to 47 genera and 29 families occurred in the 25 plots develoved, with the species diversity index (value) of 2.09 using the Shannon Wiener formula. Nine species were categorized as herbaceous plants (16.67 %) while the other 45 species were categorized as seedlings (83.33 %). The average density measured was 39 individuals/m or c. 386.400 individuals/ha. The most dominant family is Euphorbiaceae (possessing 5 diffrent species) while the most dominant species is Urochloa subquadripara (Poaceae) with and importance value of 96.08%.
PENGUJIAN PENCEMARAN DAGING BABI PADA BEBERAPA PRODUK BAKSO DENGAN TEKNOLOGI PCR: PENCARIAN SISTEM PENGUJIAN EFEKTIF Endang Tri Margawati; Muhamad Ridwan
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2055

Abstract

Entering globalization market, Indonesian government could not reject any import of food products from overseas. To anticipate the possibility of porcine contaminants into processed meat products of imported food such as meat or chicken ball, sausage, tin meat etc., it is important to apply laboratory research on such particular matter in regard to ethical and certain religious concern. This study was intended to identify the possibility of porcine contaminants into either processed meat products or fresh meat.A technique of polymerase chain reaction (PCR) was applied and PCR optimizing was conducted in advanced to obtain the right annealing temperature.Positive control of fresh pork meat was amplified to get porcine Leptin size which is 152bp fragment. Five samples of 4 meat balls and one fresh beef meat were individually collected for their DNA by either from minced or mashed after liquid nitrogen exposure then followed with a series of DNA extraction steps. PCR was assigned by using a specific primer of Leptin gene for porcine identification.Visualization of Leptin fragment was applied either on 1%, 2% of agarose gel or 10-20% gradient acrylamide gel.The result showed that all sample applied were not identified for containing porcine contaminants while positive control was on the right size of 152bp of Leptin gene. Specific primer used in this study was proved that there was not identified porcine Leptin gene on the negative control (fresh beef meat). This study suggests that a method of PCR is a simple analytical method for identification of porcine contaminants and visualization on 2% agarose gel is a cheaper and quicker method while by gradient acrylamide gel showing more clear band however this method is time consuming and expensive.
PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl Achmad Dinoto; Rita Dwi Rahayu; Aryani S Satyaningtijas
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2046

Abstract

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.
AKTIVITAS ANTIOKSIDAN DAN ANALISIS KIMIA EKSTRAK DAUN JUNGRAHAB (Baeckeafrutescens L.) Tri Murningsih
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2060

Abstract

Extraction of Jungrahab (Baeckea frutescens L.) leaves was done by using organic solvent (ether). The antioxidant activity of the extract was investigated using l,l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Ascorbic acid (vitamin C) was employed as a positif control. By virtue of their hydrogen donating ability, the extract and ascorbic acid exhibited reducing power.The extract was found has activity in free radical scavenging activity against DPPH. The antioxidant activity was supposed to be due to the composition of certain constituens e.g. 2,6-di(/-buthyl)-4-hidroxybenzoie acid (BHT-acid), 5,7-dihydroxy-6-methylflavanone and a-tocopherol (vitamin E) within the extract.
PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 Enung Sri Mulyaningsih; Hajrial Aswidinnoor; Didy Sopandie; Pieter BF OuwerkerV; Inez Hortense Slamet Loedin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
KAJIAN SUPERPARASIT DAN PREFERENSI INANG BENALU Viscum articulation Burm. f. (Viscaceae) DIKEBUN RAYA PURWODADI DAN CIBODAS Sunaryo Sunaryo
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2056

Abstract

Superparasite is a parasitic plant that lives on other parasitic plant. Viscum articulatum Burm. f. (Viscaceae) is a parasitic species of mistletoe that grows on other parasitic species of mistletoes, thus a superparasite. In Purwodadi Botanic Gardens-LIPI, East Java V. articulatum grows on Dendrophthoe pentandra and Macrosolen tetragonus. In Cibodas Botanic Gardens-LIPI, West Java it grows on D. pentandra and M. cochinchinensis. Dendrophthoe pentandra is the most infected species.The infected mistletoes are regarded as the first stage hosts.The living collections at both Botanic Gardens parasited by the three mistletoes are regarded as the second stages host.Investation preference for first and second host stages is studied in this research.
KERAGAMAN GENETIK, HERTTABILITAS DAN KORELASI BEBERAPA KARAKTER AGRONOMI PADA GALUR F2 HASIL PERSILANGAN KACANG HIJAU (Vigna radiata (L.) Wilczek) Lukman Hakim
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2047

Abstract

The F2 progenies of two crosses among three mungbean varieties were evaluated with their parents (Varsha, Park it and Local Belu)at Muara Experimental Station, Bogor during dry season of 2007. The experiment was conducted in a randomized complete block design with three replications. The seed of each F2 progenies and parents were planted in four rows of four meters long.Plant spacing was 40 x 20 cm, each will contained one plant. Among the character studies, days to maturity, seed yield per plant and plant height had the highest coefficient of variability, with the mean of 51.3%, 49.8%, and 49.2% respectively.Number of branches, pod length and number of seed per pod had the lowest coefficient of variability, and the mean were 19.1%, 18.8%, and 17.6%.The heritability estimates of nine agronomic characters studied ranged from IS.6 to 65.2%. Plant height and number of pods per planthad the highest heritability estimates with mean of 65.2% and 58.6% respectively for the two crosses. The mean heritabilityestimate for seeds yield per plant, pod length and number of seeds per pod were the lowest of 19.4%, 18.3% and 15.6% respectively. Based on the F2 data, selection of plant height and days to maturity had the highest expected genetic advance of 45.8 and 41.0%.The mean expected genetic advance for pod length and number of seeds per pod were the lowest of 19.4 and 17.4%.Correlation coefficient between grain yield with plant height, number of pods per plant and seed size were positively significant (r = 0.404, 0.613 and 0.422 respectively). Correlation coefficient between grain yield with other agronomic characters were small or negative. Therefore, plant height, number of pods per plant and seed size can be used as the selection criteria in selecting mungbean genotypes for high yield.
PENGETAHUAN LOKAL DAN PEMANFAATAN TUMBUHAN OLEH MASYARAKAT LOKAL PULAU KABAENA- SULAWESI TENGGARA Mulyati Rahayu; Rugayah Rugayah
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2052

Abstract

An ethnobotanical research focusing on the local people Moronene of the Wumbu Buro village, eastern part of Kabaena island,South East Sulawesi, was carried out using semi structural interviews and field observation. The local people practiced a traditional agricultural system. The results indicated maize Zea mays L., rice Oryza sativa L. and some other vegetable plants becoming the first priority species planted before cultivating horticultural plants such as cashew Anacardium occidentale L., cacao Theobroma cacao L. and coconut Cocos nucifera L. A total of 65 plant species were used by the local people for their daily needs, such as for food, utensil, roof, waving, medicine & cosmetic purposes including a plant species used for harvesting the palm sugar.The indigenous knowledge owned by the local people in utilizing plant species is discussed.

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