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Articles 34 Documents
Search results for , issue "Vol 10, No 4 (2011)" : 34 Documents clear
DIVERSITAS DAN HILANGNYA JENIS-JENIS IKAN DISUNGAI CILIWUNG DAN SUNGAI CISADANE Renny Kurnia Hadiaty
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.767

Abstract

The fish research in Indonesian waters has been begun since 16 century ago. Most of the research collected fish around Batavia.Many new species was described and the type specimens deposited at the museums in Europe or America.The study of fish diversity and the lost of fish species was conducted at River Ciliwung and R. Cisadane in 2009. The aim of this study is to describe the recent fish diversity in both river drainages, then make a comparison with the number of species recorded based on the references data.The differences between the two datas show the number of species lost. The result of the study showed a total of 20 species of 13 families were collected in R. Ciliwung, while from the references data there were about 187 species, so the fish diversity lost in the Ciliwung river about 92.5%. The fish research in R. Cisadane were collected 39 fish species of 24 families. There were 135 species of 38 families of the references data, so the fish diversity lost in R. Cisadane is about 75.6%.
PENGARUH PERENDAMAN BENIH DALAM CaO DAN PEMUPUKAN P DAN K TERHADAP PENGENDALIAN KERACUNAN BESI PADA TANAMAN PADI DI LAHAN SULFAT MASAM POTENSIAL Ani Susilawati; Izhar Khairullah
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.757

Abstract

Tidal land large enough sour sulfate and the potential for agricultural expansion. Obstacles encountered primarily stress iron poisoning. It has the potential to cause a decline in rice yield research aims to study the effect of the influence of seed treatment and fertilizer P and K to control iron toxicity in acidic sulfate potential land. This research was carried out in KP Belandean on MK 2007. Randomized block design with 3 replications. Rice varieties used were Batanghari, planted on plot measuring 4 mx 9 m with a spacing of 20 cm x 20 cm. Package combined treatment of seeds and fertilizer P and K: 1. (25-90-75), 2. (50-90-75), 3. (75-90-75), 4. (100-90-75), 5. (125-90-75), 6. (75-30-75), 7. (75-60-75), 8. (75-120-75), 9. (75-150-75), 10. (75-90-25), 11. (75-90-50), 12. (75-90-100), 13. (75-90-125), 14. (0-90-75), 15. (0-0-0) kg / ha% CaO-kg / ha P2O5-K2O. The result showed that by giving a dose of phosphate fertilizer 90 kg/ha P2O5 and potassium at a dose of 100-125 kg/ha K2O CaO combined with the provision of 75 % of the weight of the seed, is the combination to control iron poisoning.
KERAGAMAN GENETIKA POPULASI PELAHLAR (Dipterocarpus littoralis (Bl.) Kurz) DIPULAU NUSAKAMBANGAN BERDASARKAN PROFIL ENHANCED RANDOM AMPLIFIED POLYMORPHIC DNA Kusumadewi Sri Yulita; Tukirin Partomihardjo
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.772

Abstract

Dipterocarpus littoralis is a commercial timber species endemic to Nusakambangan island. Their existence is under severe threat due to habitat conversion and illegal logging. This study aimed to assess genetic diversity of this species within and among populations using E-RAPD. Three arbitrary RAPD primers were modified by addition of two nucleotides at their 5' termini.E-RAPD profiles were obtained by performing PCR amplification using the modified primers. Forty-five putative loci of E-RAPD were scored and analysed using POPGENE software. Some of the RAPD bands (OPA 9E-200 and 850 bp) were distinctively found for population 2, thus it served as population diagnostic marker for this population. Genetic diversity within population (0.1540) was higher than that of among populations (0.0418). Genetic differentiation (Gst) indicated that 21.35% of total genetic diversity in D. littoralis was attributed to the differences among populations. The highest genetic diversity was found in population 2(He:0.1923; 1:0.3158), while the lowest genetic variation was observed in population 1 (He: 0.0828; 1: 0.1209). Total genetic diversity for all population (Ht) was 0.1958 with an average value of genetic diversity within populations (Hs) was 0.1540. This fact suggested high level of genetic diversity found on these relic populations.
IDENTIFIKASI 26 ISOLAT BAKTERI ENDOFITIK DAN FILOSFER PADI DENGAN ANALISIS SEKUEN16S RDNA Nurul Hidayatun; Dwi N Susilowati; K Mulya K Mulya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.763

Abstract

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.
KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN) Sumaryam Sumaryam; Kusyairi Kusyairi; Sri Oetami; Hari Suprapto; Garry Cores de Vries
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.768

Abstract

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.
UJI RESISTENSI BAKTERI TERHADAP HgCl, YANG DIISOLASIDARI TANAH PENAMBANGAN EMAS DIPONGKOR, JAWA BARAT Hartati Imamuddin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.758

Abstract

Pollution of heavy metals mercury (Hg) is a serious problem especially in gold mining areas. This study aims to isolate the bacteria in the gold mining of Pongkor (West Java) and testing the bacterial resistance to mercury (Hg). The results showed that of the 5isolates tested, qualitatively only 2 isolates (P3 and P5) that resistant to HgCI2, each at a concentration of 30 and 40 ppm.Furthermore, up to 70 ppm concentration of HgCl2, P5 bacteria can still grow well even require a longer time lag phase in period.
PARTENOKARPI: BUAH TANPABIJI - APA, MENGAPADAN BAGAIMANA L Agus Sukamto
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.773

Abstract

Fruit growth and development occurred in plant for producing the offspring. Pollination and fertilization will form embryo and seed, which produce and supply plant growth regulators (PGR) for fruit growing. The role of seed can be exchanged with supply of POR exogenously and produce seedless fruit. Seedless fruit could be produced from triploid plant by crossing between tetraploid x diploid plants, in vitro culture of endosperm/irradiated pollen and genetic engineering or treated diploid plant by using PGR, pollen stress, chemical agent/ antibiotic and environment/ plant control. The benefit of triploid plant is quicker growth and produce of seedless fruits directly, whereas diploid plant could produce seedless fruits after fruit induction with special treatment. The other advantages of seedless fruit on certain plants are increased yield, fruit size/ weight,carotene and total sugar contents, decreased harvest period, yield fluctuation and blossom-end rot. Seedless fruit is preferential of consumer and give a higher price than the seeded fruit. Recently, seedless fruits of tomato, grape, citrus, cucumber and watermelon have been produced in commercial industries
PATOGENISITAS Salmonella enterica SEROTIPE enteritidis ISOLAT LOKAL PADA ANAK AYAM DAN MENCIT Ani Kusumaningsih
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.764

Abstract

This study was to identify the pathogenicity of local isolates of Salmonella enterica serotipe enteritidis (S. enteritidis/SE) in experimental chickens and mice. Thirty six local isolates of S. enteritidis isolated from chickens, eggs and human was inoculated 8 intra peritoneally (ip) to each 5 chickens and mice per group. Each group received 0.2 ml of broth culture containing 2 x 10Â CFU of S. enteritidis. Chickens and mice were observed daily at 1, 2, 3, 4, 5, 6 and 7 days after inoculation for clinical signs and mortality. The result showed that the overall mortality rate of chicken and mice were 78.4% and 63.9% respectively. The highest mortality rate was in the first day after inoculation, 51.1% for chickens and 40.0% for mice, however the lowest mortality rate was in the sixth day (0.0%) after inoculation in chickens and in the seventh day (0.6%) in mice. The bacteria of S. enteritidis can be isolated from various organs (liver, heart and spleen) and caeca tonsil of every dead chickens and mice. The study showed that local isolates of S. enteritidis isolated from chickens, eggs, and human were very pathogen for experimental chickens and mice.
STUDI PERKEMBANGAN GONAD IKAN BETOK (Anabas testudineus Bloch) DENGAN RANGSANGAN HORMON Suriansyah Suriansyah; Agus Oman Sudrajat; Muhammad Zairin Jr
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.769

Abstract

The purpose of this study was to evaluate the effect of water in oil in water (w/o/w) emulsion containing 17a-methyltestosterone on gonad development of climbing perch. The results showed administration of 200 ug/kg 17o-methyltestosterone in w/o/w emulsion enhanced gonad development of climbing perch. In this treatment, testosterone concentration increased but estradiol-17p concentration decreased. Both hormones ranged 10,55-15,13 ng/dl and 0.96-5.66 ng/dl, respectively. This treatment also accelerated the development of egg diameter which were eggs e" 0.20 mm became 71.00% after 60 days, and development of egg nucleus position into germinal vesicle breakdown (GVBD) in day 60 was about 17.50%.
STATUS INFEKSI VIRUS INFLUENZA PADA BEBERAPA SPESIES HEWAN SEBELUM WABAH AVIAN INFLUENZA H5N1 PADA UNGGAS DI INDONESIA Indrawati Sendow; RM Abdul Adj id; Paul Selleck
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.760

Abstract

After outbreak of Avian Influenza HPAI in chicken in mid 2003 in Indonesia, there was a question whether Avian Influenza HPAI was already presence in animals before the outbreak. A retrospective study was conducted to gain information on the presence of Influenza A virus infection in a range of animal species that could be infected by the virus. A total of 1529 animal sera, from 8 species from 12 different provinces which were stored at the Bbalitvet (Research Institute for Veterinary Science) Serum Bank were tested against matrix antigen of Influenza A using the Agar Gel Immunodiffusion (AGID) test. The results indicated that only 0.6% of animal tested which consisted of 4% of duck sera and 0.4% of pig sera were reacted in the AGID test with weak reaction. Those sera were then tested against Influenza A group viruses using an Enzyme Linked Immunosorbent Assay (ELISA), indicated that Influenza A viruses were not detected in either duck and pig positive sera. Those sera which were also tested by HI test against antigens of HI, H3 and H7, also indicated that none of those sera were reacted. In addition, 134 lung of pigs from an abattoir were collected for virus isolation. The viral isolation on chicken embryonated eggs resulted in 12 samples that contained viruses with agglutinated goose and chicken red blood cells. Identification of viruses isolated was done by agglutination test and ELISA. The results showed that none of those isolates were Influenza Type A virus. This study showed that influenza A virus group infection was not detected in animal species sampled before outbreak of AI H5N1 in 2003 in Indonesia.

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