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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 12, No 2 (2013)" : 30 Documents clear
ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
MORTALITAS DAN PROFIL HEMATOLOGI MENCIT YANG DIINFEKSI Trypanosoma evansi ISOLAT BANGKALAN, PEMALANG DAN PIDIE Didik T Subekti; Mutiara Febria; Febiola Rama Sari; Indri N Hartiyati
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.531

Abstract

Trypanosoma evansi known as trypanosomiasis causes or called surra in animals. Trypanosomiasis-associated death were generally due to severe anemia. Another report mentioned that a rapid death of mice can be attributed to the several reasons. One of those reasons is highly parasitaemia that leads to death of the host. However, there is another distinct report that high parasitemia in mice does not necessarily lead to anemia and clinical symptoms. This research was focused to study the relationship between trypanosomiasis-associated death with haematology profile, particularly the decrease of PCV (packed cell volume) value, anemia and virulence of T. evansi collected from several region of Indonesia. The experiments were performed by dividing mice into four groups. Three groups were infected by T.evansi according to the original isolates while another group were uninfected mice. Every two-day interval, all mice were observed their mortality and parasitaemia and also examined the PCV value and erythrocyte counts in peripheral blood. The experimental results shown that the degree of parasitemia in mice were not always related to mice mortality and anemia. Decreases of PCV value were related to the existence of parasitaemia but not with anemia. This study suggests that there is no relationship between the decreases of PCV value and anemia to the trypanosomiasis-associated death in mice.
PERAN GAMBUT TERHADAP NITROGEN TOTAL TANAH DI LAHAN RAWA Arifin Fahmi; Bostang Radjagukguk
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.536

Abstract

Peatland has important role in wetland ecosystem stability. Depletion and disappearance of peat layer lead to loss of potential source of nutrient and disruption of wetland ecosystem stability. The research aimed to study the influence of peat thickness and land hydrological condition on the total nitrogen (N) content in soil, the influence of rapidly or naturally the depletion and disappear of peat layer on total N content in soil, and the influence of hydrological condition on total N content in soil. Total N was observed in June of 2009 and 2010 (transition from wet to dry season), September of 2009 and 2010 (peak of dry season), and January of 2010 and 2011 (peak of wet season). The research was carried out on potential acid sulphate soil (A), peaty acid sulphate soil (B),shallow peat which all of peat layers wereremoved (C), shallow peat which peat layer was partially removed (D), shallow peat (E), moderate peat (F) and deep peat (G). The results showed that total N content increased with increasing of peat thickness, the depletion and disappearance of peat layer reduced total N content in soil, and declining in the groundwater level increased total N content in soil.
EVALUASI KUALITAS BEBERAPA GENOTIPE BAYAM (Amaranthus sp) PADA PENANAMAN DI JAWA BARAT Suwarni Tri Rahayu; Ali Asgar; Iteu M Hidayat; Kusmana Kusmana; Diny Djuariah
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.527

Abstract

Spinach is one of leafy vegetables species that contain lots of vitamins and minerals. This study aims to examine the quality of several genotypes of spinach grown at two highland sites of West Java (Cipanas and Lembang).This experiment was conducted using Split Plot design,with five lines and five replications. Three genotype were tested, namely B1 = By16 , B2 = By18 , and B3 = By21, as well as two commercial varieties on the market spinach (B4 = red Giti , B5 = Kusuma).Measurements included physical parameters (length and width of leaves,stem diameter, texture, and color) and chemical parameters (water content, vitamin C, and fiber). The experiment was conducted in the laboratory of Physiology Vegetable Research Institute, Lembang from March to September 2012.The results showed that the content of vitamin C and fiber in genotype B1 and B2 were not significantly different from the control genotype.Similarly, the results of measurements of the length and width parameters of the leaves on all the tested genotype was not significantly different from the control genotype. Average length and width leaves of the genotype tested in Cipanas significantly higher than those tested in Lembang. Spinach has a shelf life of 1 day at room temperature in the storage area of Lembang ( 21±2°C and 85% RH ).
CENDAWAN PENYEBAB ABORTUS DALAM ALAT REPRODUKSI SAPI BETINA Djaenudin Gholib; Riza Zainuddin Ahmad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.532

Abstract

The study on cases of abortion in cow caused by fungi have never been reported so far in Indonesia. The aim of this research is to get prevalence of cows have not and have aborted, both have reproductive disorder, and health cattles associated with percentage of fungi isolated.The study included both field survey and laboratory examination of samples. The samples of vaginal fluid were collected by using cotton swabs in cow which had suffered abortion or cow had repeated breeding, as well as freeze dried semen for artificial insemination (AI), and also examining the occurrence of abortion in cattle. The samples were collected in Sukabumi and Bandung, and then cultured in Sabouraud Dextrose Agar by stretch method, followed by incubation at 37oC. Number of samples collected consisted of 56 samples of cattle which had suffered from abortion and cow that had reproductive problems; 38 samples as control; five samples of freeze dried semen. There were no cases of abortion observed when field survey was conducted, therefore organ samples were not available for pathological and histophathological examination. Further examination on growth rate of yeast showed 20% of yeast presented in cows with abortion, 17% in cows with reproductive problem and 14% in normal cattle. On the other hand,there were samples that did not show any growth of yeast recorded f rom cows with abortion (15%)cattle with reproductive problem (7%) and normal cows (27%). Meanwhile, only twowith 2 yeast growth observed in semen samples. It was concluded that samples from cattles with reproductive problems (with and without aborted experiences) showed higher number of growth of yeasts compared with normal cow (P<0,05). Freeze dried semen were having risk of contamination by yeasts. There was no different on growth rate of fungi between reproductive problem and with abortion cow (P>0,05).
KEMAMPUAN BEBERAPA JAMUR TANAH DALAM MENGURAIKAN PESTISIDA DELTAMETRIN DAN SENYAWA LIGNOSELULOSA YB. Subowo
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.537

Abstract

Some of soil fungi capable in degrading pesticide deltamethrin, therefore they can be used as fertilizer in organic farming. As a biofertilizer ,fungus also must be able to provide nutrients for plants. The purpose of the study was to obtain fungal isolates that have the ability to decompose pesticides deltamethrin and lignocellulose compounds, dissolved inorganic phosphate compounds and produce growth hormone IAA (Indole Acetic Acid) . The fungal isolates will then be used in the manufacture of bio-fertilizers. Soil samples were put into mineral medium containing deltamethrin, fungus that grew on this medium would then be isolated. The ability of fungus in decomposing deltamethrin was observed using GC. While the ability of fungi to decompose lignocellulose, to dissolve inorganic phosphate compounds and to produce IAA was observed using a spectrophotometer. The results showed that the fungus Aspergillus niger TR1 had the ability to degrade 90.68% of deltamethrin 500 ppm after 1 h incubation and to degrade 0.46% of Poly R - 478 by after 30 min incubation. In addition, this fungus had cellulase activity of 0.029 units/ml and were able to dissolve inorganic phosphate compounds and produce IAA.
SUBSTITUSI TEPUNG BUNGKIL KEDELAI DENGAN TEPUNG DAUN LAMTORO DAN PENGARUHNYA TERHADAP EFISIENSI PAKAN DAN PERTUMBUHAN IKAN NILA Titin Kurniasih; Rosmawati Rosmawati
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.528

Abstract

The high cost of fish feed is a main problem in aquaculture industries, and the effort to improve the cost efficiency of feed is the main focus for nutritional research program all over the world. Research is needed to identify less expensive and more sustainable ingredients within fish feeds, while maintaining nutritional quality equal to those based mainly on fish meal and soybean meal. Leucaena leaf meal (LLM) is a potential alternative plant protein source because of its high protein content and low cost compared to soybean meal. The Leucaena leaf was soaked in freshwater for 3 days prior to use. This study was a 60-day trial and aimed to examine the replacement of soybean meal with LLM at various level in practical feed for Nile tilapia. This trial used a Completely Randomized Design which was carried out in 15 aquarias sized of 60 x 60 x 50 cm. Triplicate group of nile tilapia (initial body weight 12,04 ± 0,38 g fish-1 ) were fed five isonitrogenous and isocaloric feeds. The control feed was containing soybean meal, whereas in other four feeds, LLM directly replaced soybean meal with the inclusion level of 8, 16, 24 dan 31 % within the feed formulation. Result showed that the growth performance and feed efficiencies of feed containing 8, 16, 24 and 31 % LLM on the formulation were significantly lower compared to control feed. LLM was able to consumed by nile tilapia although there was a tendency of decrease of the feed consumption with the increase of LLM inclusion. It suggested that the additional treatment is needed to improve the quality of LLM, and to increase the inclusion level of LLM in feed.
PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL Evi Tahapari; Raden Roro Sri Pudji Sinarni Dewi
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.533

Abstract

Striped catfish (Pangasianodon hypophthalmus) spawning success is strongly influenced by environment.Striped catfish spawning generally occurs during the rainy season while in the dry season is difficult to find mature female. This research was conducted to improve reproduction performance of female striped catfish during dry season through combination injection of hormone pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG).The treatments were A : control (no hormone injections), B : injection of PMSG 10 IU/kg + HCG 10 IU/kg and C : injection of PMSG 20 IU/kg + HCG 10 IU/kg.Injections were done at interval of two weeks as many as six times. The research was conducted during dry season in June-October 2012 with rainfall 0.20- 14.40 mm/years. The results showed combination injection of PMSG + HCG could improve female reproduction performance. The best treatment was shown on C treatment with values of OSI (ovi somatic index), fecundity, fertilization rate, hatching rate and larva production was 12.18 %, 523 000 eggs/female, 79.65 %, 69.93 % and 290 000 larvae/female respectively. As for control treatment (A) the value of OSI, fecundity, fertilization rate, hatching rate and larval production, was 6.45 %, 197 000 eggs/female, 48.55 %, 35.59 % and 34 000 larvae/female, respectively
POLA PENYEBARAN, KELIMPAHAN DAN ASOSIASI BAMBU PADA KOMUNITAS TUMBUHAN DI TAMAN WISATA ALAM GUNUNG BAUNG JAWA TIMUR Siti Sofiah; Dede Setiadi; Didik Widyatmoko
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.538

Abstract

One of bamboo forests which are located in a conservation area in Indonesia is Mount Baung Natural Tourism Park, East Java. Bambooforest is a uniqueness/distinctiveness in this area. Study of bamboo ecology in Mount Baung Natural Tourism Park was very important inconservation purposes. The objectives of the research were to assess the distribution pattern, association and abundance of bamboo species at Mount Baung Natural Tourism Park. A systematic quadrat method was used in this study. The distribution pattern of bamboo was calculated using Morisita Index by calculating the Chi-square formula while plant association was calculated using the contingency table. The results indicated that: (1) there were 11 plant species (bearing the Importance Value Index >10%) associated with bamboo, and showing a clumped distribution pattern, (2) bamboo supported the highest importance value, in which Bambusa blumeana was the dominant. The population structure of bamboo in this area showed pre-reproductive phase, indicating the dominance of young stage (D clump < 5m).
KOMPOSISI JENIS DAN CADANGAN KARBON DI HUTAN TROPIS DATARAN RENDAH, ULU GADUT, SUMATERA BARAT Adi Bejo Suwardi; Erizal Mukhtar; Syamsuardi Syamsuardi
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.529

Abstract

Tropical lowland forest is one type of forest ecosystem that dominated most of Sumatra areal and retained carbon terrestrial within.Tropical lowland forest has the highest risk of damage than other forest types. The aimed of study is to determine species composition and carbon stock in tropical lowland forest, Ulu Gadut, West Sumatra. This study was conducted on June to October 2012 at 1 ha Pinang-Pinang permanent plot. Tree biomass was measured by using the non destructive sampling method. All trees with stem diameter at breast height (dbh) = 8 cm were measured diameter and were recorded the species of trees.As much as 852 individuals of trees, which were consisting of 45 families and 155 species with DBH = 8 cm were found in Pinang-Pinang permanent plot. Nephelium juglandifolium Blume, Swintonia schwenckii (T. & B.) Kurz,Syzygium sp.,Microcos florida (Miq.) Burret, Palaquium sp.,Cleistanthus glandulosus Jabl., Hopea dryobalanoides Miq., Mastixia trichotoma Blume, Calophyllum soulattri Burm. f. and Shorea maxiwelliana King were dominant based on -1 -1 Importance Value Index(IVI). Trees biomass and carbon stock in the study site are around 482.75 ton ha and 241.38 ton C ha respectively.

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