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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Buletin Penelitian Tanaman Rempah dan Obat Manilkara: Journal of Bioscience
Fajarudin Ahmad
Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2584

Abstract

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.
ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
Identifikasi Tingkat Ploidi Tanaman Pisang Menggunakan Flow Cytometer di Jawa Tengah Herlina; Yuyu Suryasari Poerba; Tri Handayani; Fajarudin Ahmad; Diyah Martanti
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7573.2024

Abstract

In Indonesia, banana plants are known to have high genetic diversity, both cultivated and wild bananas. Banana plants are naturally known to have several levels of ploidy, namely diploid, triploid, and tetraploid. This study aims to identify the level of ploidy in banana plants explored in Central Java. The research method used flow cytometer, which analyzes the size of the plant genome, thus providing an overview of the level of ploidy and genetic diversity in banana plant populations. The research samples were banana plant leaves from various exploration locations in Central Java. The identification results show that of the 30 banana plant leaf samples that were successfully analyzed, it is known that the largest number of samples from banana plants that have triploid ploidy level (2n = 3x), namely 15 numbers, diploid ploidy level (2n = 2x) as many as 14 numbers, and found 1 cultivated banana with tetraploid ploidy level (2n = 4x). The results of ploidy level identification can provide guidance for researchers and plant breeders in selecting the right genotype for the development of superior varieties.
Co-Authors Albertus H. Wawo Diyah Martanti Herlina L. Agus Sukamto Tri Handayani Yuyu Suryasari Poerba Yuyu Suryasari Poerba