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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 18 Documents

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Search results for , issue "Vol 17, No 1 (2018)" : 18 Documents clear

KARAKTERISASI GALUR HIBRIDA HASIL PERSILANGAN IKAN GURAMI (Osphronemus goramy Lac.) ASAL JAMBI, KALIMANTAN SELATAN DAN JAWA BARAT BERDASARKAN METODE TRUSS MORFOMETRIK [Hybrid Strain Characterization Result of Crossbred Giant Gouramy (Osphronemus goramy Lac.) Origin of Jambi, South Kalimantan and West Java Based on Morphometric Truss Method] suharyanto, suharyanto; Febrianti, Rita; Sularto, Sularto; Abimanyu, Ade Anom
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i1.2845

Giant gouramy is a native fish to Indonesia and widely distributed in Sumatra, Kalimantan and Java. The aim of this study was to characterize the phenotype and kinship of Giant Gouramy from crosses between strains Jambi, South Kalimantan, and West Java (Tasikmalaya). This Research was conducted at the Giant gouramy Hatchery of Research Institute for Fish Breeding, Sukamandi, West Java. Analysis of morphological diversity of all crosses between varieties performed through morphometric measurements that have been determined based on a point benchmark of 16 haracteristics of the truss. Measurement data of each character crosses which have beenrelativized by standard length were analyzed using discriminant analysis and hierarchical cluster analysis. The result showed that Giant gouramy has low diversity coefficient values, ranging from 4 to13%. The highest coefficient of variance (CV) was found in the character of C3 (the tip of the anal fin-base of the tail fin), which ranges from 18-33% and the lowest in the character B5 (the base of the pelvic fin-tip of the dorsal fin) in the range of 2-4%. Based on discriminant analysis, males Giant Gouramy have more character differences than females. The closest kinship relationship is in hybrid of Tasikmalaya and Jambi (TJ) strain with of South Kalimantan and Tasikmalaya ( KT) which has a similarity level at 89.41%. However, the farthest kinship is in pure line of Tasikmalaya (TT) and Jambi (JJ) with the three other strains namely South Kalimantan and Jambi (KJ), Tasikmalaya and Jambi (TJ) and South Kalimantan and Tasikmalaya (KT) with a value ofsimilarity of 53.93%.

PERTUMBUHAN IKAN BANDENG (Chanos chanos) ANTARA BENIH HSRT DAN GENERASI KEDUA (G-2) TERSELEKSI [Growth Performance of Milkfish, (Chanos chanos) Between Small Scale Hatcheries and of Selected Second- Generation (G-2) Sources] Kusumawati, Daniar; Jamaris, Zafran; Aslianti, Titiek
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Currently, there is a national concern regarding to the decrease of milkfish production from ponds in North Java due to a low quality ofÂ milkfish seed produced by small scale hatcheries in Bali, which is the main producer of milkfish seed. The quality of seed is strongly relatedÂ to the quality of eggs (fertility), while quality of eggs produced depends on the management of brood stocks carried out by theÂ hatcheries. Growth rate and growth hormone profiles are some of the biological aspects that could be used as a basis/benchmark to evaluateÂ quality level of milkfish seed reared in ponds. The aim of this experiment was to evaluate growth rate and growth hormone profileÂ performance between selected G-2 seed and small scale hatcheries seed as control population the growth rate. Research on grow-out ofÂ milkfish seed was conducted at the IMRAD ponds facility in Pejarakan, using milkfish seed produced by small scale hatcheries as well asÂ selected second-generation (G-2) seed, each with the density of 5000 seed/pond (1 pond=0.5 ha). The seeds were fed with dry pellet andÂ reared for 5 â 6 months. The results showed that the seed produced with standard operational procedure (SOP) by small scale hatcheriesÂ were having longer (F hit. = 13.68 > F tabel 1%) and heavier body washt (F hit. = 18.98 > F tabel 1%) better than selected G-2 seed andÂ small scale hatcheries seed without SOP with high growth hormone concentration (F hit. = 4.95 > F tabel 5%).

KAJIAN ETNOBOTANI RAMUAN PASCA MELAHIRKAN PADA MASYARAKAT ENGGANO [The Ethnobotanical Study of Postpartum Concoction on Enggano People] Royyani, Mohammad Fathi; Lestari, Vera Budi; Agusta, Andria; Efendy, Oscar
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research was aimed to discover the traditional knowledge of Enggano people when using plants as concoction after giving birth. Data was collected through in depth interview, open-ended, and literature studies. The results showed that Enggano people used specific plants as concoction in childbirth process. There were two types of the concoction. The first was used after giving birth to 40 days, jarak pagar or tiarak (Jatropha curcas L.). The second was used after 40 days to 60 days or after recovery, such as dukung anak (Phyllanthus niruri Linn.), kumis kucing (Orthosiphon stamineus Benth.), temu lawak (Curcuma xanthoriza Roxb.), alang-alang (Imperata cylindrica (L) P. Beauv.), banana (Musa sp.), kencur (Kaempferia galanga L.), turmeric (Curcuma longa L.), and ginger (Zingiber officinale Roscoe). Results from our literature studies confirmed the efficacy of plants used by Enggano people is scientifically acceptable. Further study on phytochemical aspects of these medicial plants is therefore required to be carried out.

AKTIVITAS ANTIBIOFILM BAKTERI Escherichia coli OLEH BAKTERIOFAG SECARA IN VITRO [Escherichia coli biofilm in vitro eradication by bacteriophage] Triana, Evi
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Several Escherichia coli strains are pathogenic. Excessive and noncompliant use of antibiotics and disinfectants may cause bacteria to build resistance mechanisms. Forming biofilms cause eradicatation more difficult. An effective cleaning action required antibiofilm and antimicrobial agents that have different mechanisms with antibiotics and disinfectants. Bacteriophages are potential candidates because they meet these requirements. Bacteriophages produce specific polysaccharide lyase enzymes capable of degrading biofilm extracellular polymeric matrix. Study was aimed to determine concentrations of specific bacteriophage showing Escherichia coli antibiofilm activity was conducted. The results of this study showed that the most effective concentrations bacteriophage EC RTH 04 to prevent, inhibit, and degrade Escherichia coli EC 3 biofilms were 106, 102, dan 102 respectively.

KERAGAMAN IKTIOFAUNA MUARA SUNGAI CIMANUK, INDRAMAYU, JAWA BARAT [Ichthyofaunal Diversity of Cimanuk Estuary, Indramayu, West Java] Tampubolon, Prawira ARP; Ernawati, Yunizar; Rahardjo, M.F.
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Cimanuk river, which the estuary formed a delta, is a habitat for many fishes that occupied the water in northern coast of Ja va. The estuary is essential for supporting the fish life cycle. The aim of this study was to asscess the diversity of ichthyo fauna in Cima nuk River estuary. The fishes were collected in three months from July to September 2013 at three locations: Pagirikan, Pabean Ilir and Song. Total fish collected were 1,826 individuals, consisted of 103 species from 41 families and 14 orders. Most of them were from Family Ambassidae,Leiognathidae, Scianidae, Gobiidae, and Ariidae.

KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING [Genetic Characterization of African Catfish Revealed by RAPD Fingerprinting Markers] Nugroho, Estu; Putera, Sabara
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 â 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 â 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.

KEANEKARAGAMAN, PERSEBARAN DAN POLA TATA RUANG TUMBUHAN EPIFIT PADA HUTAN BEKAS TEBANGAN DI KIYU, PEGUNUNGAN MERATUS, KALIMANTAN SELATAN [Diversity, Distribution and Spatial Patterns of Epiphytic Plants at The Logged Over Forest in Kiyu Forest, Meratus Mountain, South Kalimantan] Sadili, Asep; Royyani, Mohammad Fathi
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A quantitative study of epiphytic plants was conducted at the logged over forest in Kiyu, Meratus Mountain, Hulu Sungai Tengah, SouthÂ Kalimantan. This study used a plot of 10 m x 500 m (Â± 0,5 ha), and divided into 50 subplot of 10 m x 10 m. The results showed that thereÂ were nine species of six genera from three families of epiphytic plants found within the studied plot, with A grostophyllum bicuspidatum asÂ the dominant species. The diversity index (Hâ) was of 1,85. The diversity of epiphytic plant in these plots was well represented based on theÂ results of analysis of species area curve. Spatial distribution pattern of all epiphytic species was homogeneus, except forÂ A. bicuspidatum. The number of epiphytic host plants observed was 22 individuals from six species of six genera, and five families, withÂ Saurauia nudiflora (Acthiniaceae) was the dominant host plant.

POTENSI VEGETASI DAN DAYA DUKUNG UNTUK HABITAT GAJAH SUMATERA (Elephas maximus sumatranus) DI AREAL PERKEBUNAN SAWIT DAN HUTAN PRODUKSI KECAMATAN SUNGAI MENANG, KABUPATEN OGAN KOMERING ILIR [Vegetation Potency and Carrying Capacity for Sumatran Elephant (Elephas maximus sumatranus) Habitat at Palm Oil Plantation and Forest Production Area in Sungai Menang Districts, Ogan Komering Ilir Regency] Setiasih, Garsetiasih; Rianti, Anita; Takandjandji, Mariana
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Forest land converted into palm oil plantations have caused habitat fragmentation of elephant and land degradation. These lead to land use conflict between human and elephant. The conflict often caused the elephant killed and destructed agricultural land. The study was aimed to estimate potential use and carrying capacity of elephant habitat. Data collection of undergrowth vegetation were analyzed using twelve square plots of 1 x 1m, the distance between the plot of 50 m, tree vegetation of seedlings size 1 x 1 m, saplings 5 x 5 m, and trees 20 x 20 m, the distance between the plot of 200 m and of 1000 m lenght. Vegetation used as elephants feed were observed using purposive sampling and systematically procedure. The analysis showed that biomass of plants producing elephant fodder in Tambang Besi were of Cyperus rotundus (3600.26 kg/ha), Cynodon dactylon (346.74 kg/ha), Melaleuca leucadendron (255.21 kg/ha), and Melastoma malabatricum (156.40 kg/ha). While, the highest biomass in Tebing Penigasan plot is Cyperus rotundus (3575 kg/ha), and in Barak Gajah Plot is Isachne globusa (4013.33 kg/ha). The carrying capacity of elephants habitat of Tambang Besi, Tebing Penigasan, and Barak Gajah plots are 0.78, 0.29, and 0.41 individual/ha/day, respectively.Â

PENGARUH SUMBER PUPUK ORGANIK TERHADAP PENAMPILAN TEBU (Saccharum officinarum L.) PADA TATA TANAMBARIS GANDA BENIH GANDA [Effect of Organic Fertilizer Resources on Sugarcane (Saccharum officinarum L.) Performances in Double Rows Double Seeds Planting Arrangement] Busro, Djumali; Mulyaningsih, Sri; Basuki, Teger
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Increased productivity of sugarcane in upland area can be done through the implementation of the integration of manure with a high dose of inorganic fertilizer in the double rows and twice plant populations of single row (âdouble rows double seedsâ) planting arrangement. Limited availability of manure and inorganic fertilizer entails the replacement of manure with green manure fertilizer. The research wasÂ aimed to obtain a green manure as replacement manure that can be intercropped in the double rows double seeds planting arrangement. The experiment was done in the Asembagus Experimental Station, Situbondo, East Java on January to December 2014 for plant cane (PC) and from January to December 2015 for ratoon cane (RC). Treatments were arranged in a Randomized Block Design time for replication. The treatment includes (1) the four rows of Crotalaria juncea, (2) two rows of Crotalaria juncea, (3) four rows of peanuts in width between rows of sugar cane, (4) manure, and (5) no organic fertilizer. The results showed that the integration of organic fertilizer with a low dose of inorganic fertilizers increases productivity and sugar yield 5.20 to 41.78% and 33.33 to 69.90%, respectively in PC and 7.33 to 32.67% and 5.74 to 29.61%, respectively in RC from no organic fertilizer. Four rows of Crotalaria juncea could replace the role of manure in improving the productivity and sugar yield for PC and two rows of Crotalaria juncea for RC.

KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING Estu Nugroho; Sabara Putera
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i1.2869

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 – 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton 0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 – 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.

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