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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 8, No 6 (2007)" : 30 Documents clear
MUTASI STRUCTURAL INTRON trnL (UAA) PADA SUKU MERANTI-MERANTIAN (DIPTEROCARPACEAE) Kusumadewi Sri Yulita
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.823

Abstract

tRNA " UAA (trnL) intron have been widely utilised for phylogenetic reconstruction in angiosperm families. Two main helical elements of secondary structure of trnL intron (stem loop regions P6 and P8) contained the most variable sequences across taxa.Detailed examination on the insertion-deletion events in trnL intron sequences of 110 species of Dipterocarpaceae has identified 4 stem loop structures within both loops.The first stemJoqp was found in P6 loop and three other were discovered in P8 loop.These structures featuring characteristic for genericJinfra-generic level depended upon which taxonomic classifications are foJJowed.One of these structures formed a large loop of 72 nucleotides and was a unique major stem loop in Dipterocarps family whose loop was the major structural mutation of trnL intron in this family.
PROTEIN TOKSININSEKTISIDAL DARI BAKTERI PATOGEN SERANGGA Photorhabdus luminescens HJ Alina Akhdiya; Etty Pratiwi; I Made Samudra
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.828

Abstract

Photorhabdus luminescens HJ is an entomopathogenic bacterium that has a high toxicity against Tenebrio molitor larvae.Toxicity assay of crude extra cellular protein precipitated using ammonium sulphate showed that the highest toxin activity was found in 70 % saturation. Purification of the toxin using Hi Prep 16/60 Sephacryl S-200 HR column exhibited one fraction of toxic protein and three fractions of non-toxic protein. Mortality of T. molitor larvae treated with 19.2 nanogram of toxic fraction was up to 80%. Denatured protein analysis using sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that the toxic fraction was composed of three proteins, which were 19.5, 42, and 66 kDa respectively. Based on toxin activity bioassay, this toxin type was an injectable toxin and presumably classified as Mcf toxin.
FISIOLOGI BUI DORMAN GEWANG (Corypha Man Lamarck) BP Naiola
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.836

Abstract

Gewang (Corypha utan Lamarck), a wild or semi-wild palm species distribute widely in Nusa Tenggara savanna with great potential(as building materials, drink and food), as traditionally have been exploited and utilized by local villagers for ages.To increase her potential should be promoted by plant domestication applied as on farm model such as agroforestry and social forestry. Seedlings availability is one of the basic need when comes to plant domestication. The only possibility of propagation in gewang is by generative phase (seeds). On the other hand, gewang seeds are known as orthodox, with low percentage of germination. This study shows that mature gewang seeds dropped under the mother trees are highly protected by tight tissues of endocarp and endosperm. Although the mature seeds in the nature seems ready to germinate, however there should be a dimensional balance (chemical, physical and physiological) or synchronization among components (endocarp/testa, endosperm and embryo) as a driving force to promote germination.In nature, the attainment of this synchronization/ dimensional balance of gewang seeds, are driven by external (both biotic and abiotic) factors as well such as fungi and climatic fluctuations. While artificial treatments like scarification, soaking, heating and acid are to accelerate the dimensional balance. Low embryo content per 100 seeds also responsible for low seed germination. It is suggested that low embryo content may be due to interactions among factors like low fertility of pollen grains, time incompatibility between gynoecium (stigma) and androecium (pollen grains) during pollen shed process and ineffectiveness of pollination due to great number of flowers.
PENGELOMPOKAN PLASMANUTFAH SPESIES PADI LIAR {Oryza spp.) BERDAS ARKAN PEUB AH KUANTITATIF TANAMAN Tintin Suhartini; Sutoro Sutoro
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.824

Abstract

Wild rice species are important gene sources for the rice improvement programs.The morphological characters is the easiest way to identify the crop specificity, and it is applicable for determining the relation among species.The grouping on wild rice species are determined by using cluster analysis through the principal component analysis which involved 16 characters of quantitative traits of wild rice species. The total sample of wild rice species was 89 accessions of 18 species.The research was done at green house level during 3 seasons from 2004 to 2005.All the accessions of wild rice were planted in pots consists of 10 kg soil by 3 replications for each accession.The result of analysis of the first principal component from quantitative characters (plant height,amount of grain fill per panicle, amount of grain total per panicle, panicle length, 1000 grains weight, grain shape, days of flowering, total internodes and awning length ) could explain that variability were 82 %.The result of cluster analysis involved the four of principal component with similarity level equal to 80 %, obtained 5 groups of wild rice species. The result of the clusters was Oryza sativa included at cluster I, while O. officinalis had 3 clusters that is cluster III, IV and V. While O. meyeriana and Oryza ridleyi joint at cluster II.Quantitative character was applicable for clustering the accessions of wild rice species as according to each genome.
PENGETAHUAN TRADISIONAL DAN PEMANFAATAN TUMBUHAN OLEH MASYARAKAT LOKAL PULAU WAWONII SULAWESI TENGGARA Mulyati Rahayu; Rugayah Rugayah
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.829

Abstract

Wawonii is one of the small islands located at S.E. Celebes. Plant Diversity and its utilization by the local people have not been recorded yet.Ethnobotanic study has been conducted in several location (Lansilowo, Wawolaa, Lampeapi and Boboloi Districts) visited in 2003-2005, using semi structural interviews and open-ended method. The result of trie study showed that community of this island consist of several ethnics (Bajo, Menui, Muna, Buton, Tolaki. Kolesusu, Jawa and Wawonii).Wawonii is the origin and majority ethnic in this island. About 200 species of plant have been used for their daily need such as for food, utensil, medicine and cosmetic, waving, energy and others.Indigenous knowledge of the local people about the species plant utilization will be discussed.
JAMUR ARBUSKULA PADA BAMBU DIJAWA Kartini Kramadibrata; Hadi Prastyo; Agustin Widya Gunawan
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.837

Abstract

An attempt to produce arbuscular fungi (AF) spore in pot culture from bamboo soil samples collected from Meru Betiri National Park, Jember, East Java and Purwakarta, West Java was carried out by used of Pueraria phaseoloides as a host plant. The result showed nine species of AF i.e. Acaulospora foveata associated with Dendrocalamus asper and Gigantochloa apus, A. scrobiculata associated with D. asper and G. apus, A. tuberculata associated with Bambusa vulgaris, D. asper, Schizostachyum zollingeri, G.manggong and G. apus. Glotnus etunicatum associated with B. vulgaris, D. asper, G. manggong and G. apus while G. fuegianum associated with B. vulgaris, D. asper, S. zollingeri, Glomus cf. formosanum and G. geosporum, associated with D. asper and G. apus. G. mosseae associated with S. zollingeri and G. apus and Scutellospora calospora associated with G. manggong.The association of some species of AF with several species of bamboo in Java found in this study is apparently new record.
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Yati Sudaryati Soeka; Elidar Naiola; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
PENGAMATAN INFEKSI JAMUR PATOGEN SERANGGA Metarhizium anisopliae (Metsch. Sorokin) PADA WERENG COKLAT Y Suryadi; Triny S Kadir
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.830

Abstract

Observation on infection of fungus entomopathogen Metarhizium anisopliae on insect brown plant hopper was carried out using scanning electron microscope (SEM).The infection of M. anisopliae on insect bodies was shown by conidia sporulation on divergent chain with conidia size of 1.6 x 7.8 urn. Based upon SEM observation, the results revealed that fungus hyphae of M.anisopliae was found on insect body i.e. on cuticle as well as on segment between abdomen, legs and insect facet eyes.In epizootic condition, the cadavers were shown can act as source of fungus dissemination on healthy insect when environment (temperature and humidity) was suitable for primary infection.
KERAGAMAN FENOTIPE RAPD Santalum album L. DIPULAU TIMOR BAGIAN TIMUR Yuyu S Poerba; Albert H Wawo; KS Yulita
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.838

Abstract

Santalum album L. (sandalwood/cendana) is known as one of medicinal and aromatic tree species in Indonesia. The species is valued for its quality light wood timber and for its medicinal properties.The species has been overexploited and is considered as vulnerable plant species.The present study aimed to assess genetic diversity and to estimate genetic relationship among 58 accessions of plant germplasm collection using Random Amplified Polymorphic DNA (RAPD).Two RAPD primers generated 34 scorable bands with 97.06% of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method.The range of genetic dissimilarity value among species was from 6% to 91%, while the range of genetic distance between populations was from 1.89% and 26.88%.These values showed that 5.album from Eastern part of Timor was genetically diverse populations.Within the 12 populations, there were 9 banding patterns recorded from primer OPA 16 and 12 banding patterns from primer OPB 12, suggesting that OPB 12 was more sensitive than that of OPA 16 to show variation within the sample used.
KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Otong Zenal Arifin; Estu Nugroho; Rudhy Gustiano
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.826

Abstract

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).

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