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BERITA BIOLOGI
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Articles 26 Documents
Search results for , issue "Vol 9, No 1 (2008)" : 26 Documents clear
KARAKTERISASI 17 FAMILI IKAN NILA {Oreochromis niloticus) GENERASI KE TIGA (G-3) BERDASARKAN METODE TRUSS MORFOMETRIKS Nuryadi, Nuryadi; Ariiin, Otong Zenal; Gustiano, Rudhy; Mulyasari, Mulyasari
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1189.01 KB) | DOI: 10.14203/beritabiologi.v9i1.806

Abstract

Objective of the study is to elucidate phenotype characters of nile tilapia (Oreochromis niloticus) of the third generation (G3)resulted from the selection breeding program in the Research Institute for Freshwater Aquaculture Bogor. Phenotypes of seventeen nile tilapia families, were observed using truss morphometric methods. The results showed that the composition average of standard lenght has low coefficient variation mean (10.42%) as well as coefficient variation of the truss measured characters (4.3- 13.3%). The highest index similarity of boddy shape within family was found in family 5 (79.3%), in contrast the lowest was in family 12 (32.3%). For index similarity between families, the highest score was in between family 12 and 17 (22.6).There were 8 characters enabled to differentiate the 17 families observed. They were Al, A3, A5, B2, C3, C5, C5, and D4. Cluster analyses recognized into 4 groups based on the phenotypic distance
RETRANSFORMATION AND EXPRESSION OF RECOMBINANT VIRAL PROTEIN OF JEMBRANA JSU AND JTat (JSU AND JTat) IN pGEX SYSTEM [Retransformasi dan Ekspresi Protein Virus Rekombinan JSU dan JTat Penyakit Jembrana dalam Sistem pGex] Margawati, Endang T; Utama, Andi; Indriawati, Indriawati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.793 KB) | DOI: 10.14203/beritabiologi.v9i1.802

Abstract

Genom virus penyakit Jembrana setidaknya memiliki 3 gen besar yang menyandi protein dan beberapa di antaranya diperlukan untuk replikasi virus. Protein JSU dan JTat diduga dapat menginduksi kekebalan yang protektif pada sapi Bali terhadap penyakit Jembrana sehingga keduanya sangat berpotensi untuk dipakai sebagai vaksin rekombinan. Penelitian ini dirancang untuk meretransformasi protein rekombinan JSU dan JTat ke dalam Escherichia coli menggunakan sistem pGEX. Konstruk JSU dan JTat dalam pGEX dikoleksi plasmidnya dengan metode miniprep dan kemudian diretranformasikan ke dalam E. coli strain BL21 dan DH5a. JSU dan JTat hasil retransformasi diekspresikan pada medium LB untuk skala produksi kecil dengan sistem pGEX. Hasil penelitian ini meminjukkan bahwa kedua JSU dan JTat hasil retransformasi ke dalam E. coli strain BL21 terlihat tumbuh lebih baik pada medium LB jika dibandingkan retransformasi ke dalam E. coli strain DH5a. Hasil retransformasi JSU dan JTat dikarakterisasi dan diidentifikasi dengan Western blotting dan tampak menunjukkan ukuran protein yang benar, yaitu protein rekombinan JSU berukuran 60kDa dan JTat berukuran 36,7kDa. Protein rekombinan JSU muncul dengan pita tunggal dan lebih jelas jika dibandingkan dengan protein JTat. Konsentrasi protein JSU sedikit lebih rendah (1,883 mg ml ) jika dibandingkan dengan JTat (l,981mg ml).Penelitian ini menunjukkan bahwa JSU pGEX masih tersimpan dan diekspresikan dengan baik, sementara JTat mungkin perlu dilakukan perakitan ulang untuk memantapkan ekspresinya.
INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

Abstract

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.
POPULASI POHON JENIS DIPTEROCARPACEAE DITIGA TIPE HUTAN PAMAH KALIMANTAN Herwint Simbolon
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.803

Abstract

Data of Dipterocarpaceae species have been extracted from 8 studied plots of 1-ha each, distributed in three types of natural forest in Kalimantan (those were 4 plots in mixed dipterocarps forest, 2 plots in peat swamp forest and 2 plots in heath forest types) for further population analysis. Number of Dipterocarpaceae species in mixed dipterocarps forest type was higher than of in two other forest types, however mean number of individual per species was higher in peat swamp and heath forest types than of in mixed dipterocarps forest type. Some of Dipterocarpaceae species are adapted to heath and peat swamp forest type conditions indicated by the number and distribution of population of the species in both forest types. The higher the population number of juvenile trees 5-10 cm in diameter, the lower population number of mature trees of diameter 50cm up, indicated the higher tree population the higher mortality rate. However, survivorship of tree will higher as the tree diameter higher. Survivorship of trees in mixed dipterocarp forest is higher than of in peat swamp and heath forest types.
POTENSI ANTIBAKTERIA EKSTRAK DAN FRAKSI LIBO (Piper miniatum BL) Sumarnie -H Priyono
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.808

Abstract

Libo (Piper miniatum Bl.) is traditionally used in Papua as spices and tonic. Besides as food flavor, it is commonly used as food natural preservative that is related to its antibacterial activity. But so far, there is no scientific report about antibacterial activity from the plant extract.The antibacterial activity test result in this study, showed that the four extracts (n-hexane, chloroform, ethyl acetate and methanol) of libo were active against Streptococcus mutants (BCC1863) and Staphylococcus aureus (ATCC 25923). The extracts were active only on high consentration againts Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922), meanwhile on Salmonella enteriditis (BCC 2754), Staptylococcus epidermidis strain Cowan (BCC 1820) and Bacillus subtilis (ATCC 10400) gave different antibacterial effect. The n-hexane extract has the highest antibacterial activity againts S. mutans (BCC 1863). Fractionation result of n-hexane extract, F3 and F4 at concentration 124.4|ig showed antibacterial effect against Listeria monocytogenes (M 19117) equivalent to erythromycin 10 \\g as standart antibiotic. While Fl and F2 at concentration 124.4|ig had antibacterial activity against E. coli (ATCC 25922) equivalent to erythromycin 10 ug.
KERAGAMAN AKTINOMISETES KEPULAUAN WAIGEO, KABUPATEN RAJA AMPAT, PAPUA DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSADAN PELARUT FOSFAT Arif Nurkanto
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.776

Abstract

Some Actinomycetes isolated from Waigeo Raja Ampat Regency Papua have been identified. Those isolates were also characterized for their cellulolitic and phosphate solubilizing ability. Microscopic identification was based on Miyadoh (1997) and Holt (1994) methods.Actinomycetes could be identified by microscopic observation on spores, chain spore, hypha, aerial hypha and its pigmentation.The cellulolitic ability was observed by clear zone ratio in CMC medium and phosphate solubilizing activity by the same approach in Vikoskaya medium.From 139 Waigeo's Actinomycetes isolates which had been deposited in LIPI Microbial Collection (LIPIMC) were identified as 10 genera (Actinomadura, Actinoplanes, Microbiospora, Micromonospora, Nocardia, Pseudonocardia, Saccharopolispora, Streptomices, Streptosporangium and Thermomonospora). As much as 57.5% phosphate solubilizing actinomycetes and 82.7 % cellulolitic actinomycetes were detected.
DAUR PATOLOGIS TEGAKAN HUTAN TANAMAN Acacia mangium Willd. Simon Taka Nuhamara; Soetrisno Hadi; Endang Suhendang; Maggy T Suhartono; Wasrin Syafii; Achmad Achmad
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.804

Abstract

Heart rot on Acacia mangium Willd. forest stand is critical especially for mechanical or construction wood based purposes. Failure on understanding the nature and the way it get established into the tree stem may cause high economic consequences.Anticipating such a worse condition, studies on cull factor in relation to age was initiated. Eventually the study is aimed at healthy cutting cycles of the clear and purposely stand establishment. The study had been carried out at BKPH Parung Panjang, KPH Bogor. Following the cull factor measurement technique combined with the pathological rotation estimation procedures, it was found that the healthy volume was 0.0623 m and the cull factor was 31.25 %.The figures were at the age of nine years. Therefore, the pathological rotation cycle for the A. mangium stand in the area could be fitted at eight years, as being adopted so far.This is true, when the plantation is established for the production of wood, provided that the tending operation is optimal. Applying the United States Department of Agriculture (USDA) Forest Health Monitoring (FHM) indices, the general performance of the A. mangium forest stand in Parung Panjang is found to be in healthy criteria. The damage indices for all stand ages investigated varied from 2.77 (lowest) to 5.16 (highest) as compared to the 21.18 value, the possible highest FHM tree index.
TOLERANSI SENGON BUTO (Enterolobium cyclocarpum Griseb) YANG DITANAM PADA MEDIA LIMBAH TAILING TERCEMAR SIANIDA DENGAN PERLAKUAN PUPUK Fauzia Syarif
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.809

Abstract

Sengon buto (Enterolobium cyclocarpum Griseb) is one of the potential plant species for land reclamation and possibly for phytoremediation because of its fast growing even on poor soil, and its ability to fertilize soil through nitrogen fixation. For phytoremediation purpose we need fast growing plant with high ability to absorp target contaminant. Fertilization is needed to improve plant growth. This experiment was carried out to study the tolerance of sengon buto (Enterolobium cyclocarpum Griseb) grown on cyanide contaminated tailing media on various level of NPK fertilizer.The sengon buto seed were planted in the tailing of gold mine media mixed with compost (4:1) in pots. Various levels of NPK fertilizer were applied i.e. 0, 2, 4, 6, 8 g/pot at 2 and 2.5 month after planting (MAP).The plant were harvested at 6 MAP. The result showed that NPK fertilizer increase plant growth at level 2 of 2 g/pot, however the value of the increment was not significantly different with control plant (0 g/pot). Cyanide content of NPK treated plants were higher than the untreated plants. The highest value of shoot/root cyanide content ratio was on the NPK untreated plants (4.34) followed by 2 g/pot treatment (3.59). It is means that sengon buto is potential for phytoremediation of tailing of gold mining.
POTENSI IKAN MUJAIR (Sarotherodon mossambica) SEBAGAI BIOAKUMULATOR PENCEMARAN PESTISIDA PADA LINGKUNGAN PERTANIAN Yulvian Sani; Indraningsih Indraningsih
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.800

Abstract

An analysis of pesticide contamination in farm sites was conducted to investigate the impacts of organochlorine contamination in environmental matrices leading to pesticide residue in animal products and to identify a bioaccumulator of freshwater (mujair) fish (Sarotherodon mossambica).The observation sites were selected in accordance to the presence of animal farms within the agricultural areas applying pesticides intensively, such as Bandung, Sukabumi and Bogor districts. Samples consisting of water, soils,animal feed, freshwater fish, poultry meats, eggs and weeds were collected from these areas. The study shows that some organochlorines - OC (lindan, endosulfan and DDT metabolites) were detected from all samples. Endosulfan and lindan appeared to be used extensively for horticulture activity (corn, chilly, cassava and tobacco) in these areas, where both pesticides could also be detected either from animal products (meats and eggs) or water, freshwater fish and soils collected from the same areas.The results reveals that there were positive correlation between OC contamination in the environment and residues in animal products. The weed of babadotan (Ageratum conyzoides) was found growing abundantly around the animal farms and was able to accumulate the pesticides. Furthermore, freshwater mujair fish (S. mossambica) appeared to reduce endosulfan contaminantion in water as indicated by an increase pesticide residues in its tissues.
KEANEKARAGAMAN FLORA CAGAR ALAM NUSABARONG, JEMBER - J AWA TIMUR Tukirin Partomihardjo; Ismail Ismail
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.805

Abstract

Flora of Nusa Barong Nature Reserve, Jember-East Java, was intensively surveyed in 2005. At least 357 specimens consist of 282 species belonging to 232 genera and 88 families have been collected during the survey. Those are includes four species of Pterydophytes and 278 species of Spermatophytes. Among them there were three protected species Corypha utan, Excoecaria agalloca and Protium javanicum. Based on the IUCN categorization status, there were five species under threatened condition, i.e.Agalia edulis (LR/nt), Casearia flavovirens (VU.Bl+2c), Cycas rumphii (NT decreasing), Intsia bijuga and Intsia palembanica,both under VU.Al.cd.The natural population of these last two species was drastically decreasing due to the international trade. Most of the species recognized as new records for the island since there was no complete flora record of the area. Further studies are needed to gain more complete biodiversity information of small island ecosystem.

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