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All Journal Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia BERITA BIOLOGI ANNALES BOGORIENSES Bioma : Jurnal Biologi Indonesia Jurnal Litbang Kota Pekalongan

ROSSA YUNITA

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111, Indonesia Telp. (0251) 8337975

Author-ID : 425890

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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Articles

REGENERASI TUNAS DARI KALUS YANG TELAH DIBERI PERLAKUAN IRADIASI PADA PADI VARIETAS FATMAWATI [Shoot Regeneration of the Fatmawati Rice Variant Radiated Callie] Yunita, Rossa; Lestari, Endang G; Dewi, Iswari S
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Gamma ray mutative induction for increasing genetic variation has been applied for plant prime variety engineering. The materials are derrived from seed organ, shoot and calli. Calli is a group of actively dividing cell and have not been organized to form plant. The benefit of using calli explant is that the gamma ray could directly shot to DNA in the nuclear cell in such a way that there is higher opportunity for genetic change to occur. The problema of using calli explant are the difficulties in regenerating the calli into shoots, due to the deformation as a result of radiation process. Therefore, this research is aimed at obtaining the appropriate media formulation for shoot regeneration from Fatmawati-rice calli which has been irradiated with gamma ray. The reseach was conducted in BB-Biogen laboratory consisting of three experiments, such as : (1) calli iradiation with the dosage of 0; 5; 10; 15; 20; 25; 30; 35; 40; 45; 50; 55 and 60 Gy, (2) shoot regeneration at the MS + BA (0, 1, dan 3 mg/l) + IAA (0 dan 0,8 mg/l) media, and BA (0, 1, and 3 mg/l) + zeatin ( 0; 0,1; 0,2 and 0,3 mg/l) + IAA 0,8 mg/l and (3) Shoot induction at MS + IBA (0, 1, 2 and 3 mg/l) media. The result shows that the range of LD50 was obtained at the dosage of 30 Gy, the most apropriate media for shoot regeneration is MS + BA 3 mg/l + IAA 0,8 + zeatin 0,1 mg/l and media for root induction is IBA 1 mg/l.

INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.

Identification Of Aquatic Plant Species From Bantimurung Waterfall And Their Phytochemical Compounds Analysis Nugraha, Media Fitri Isma; Maharani, Fasya Hadaina; Novita, Hessy; Rajamuddin, Muh Alias L; Yunita, Rossa; Reflinur, Reflinur; Enggarini, Wening; Julzarika, Atriyon; Elya, Berna Elya
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

A total of 21 aquatic plant species were collected from Bantimurung waterfall areas, South Sulawesi, Indonesia, in October 2017. These plant materials were subjected to both species and phytochemicals identification. The aims of this study were to determine the species or taxonomic rank of Indonesian aquatic plants collected from Bantimurung waterfall, South Sulawesi, Indonesia and to identify their chemical compounds (phytochemicals) as a candidate for new herbal medicine. Plant genetic materials used in this study were collected from Bantimurung Bulusaraung waterfall and were then identified based on standard botanical techniques for species identification in the Herbarium Bogoriense, Research center for Biology Indonesian Institute of Science (LIPI), Cibinong, West Java. The samples were subjected to the phytochemistry screening such as alkaloids, flavonoids, tannins, saponins, glycosides, terpenoids and anthraquinone followed the procedures of Indonesian Materia Medika and Harborne. Results showed that all collected aquatic plant samples were able to be identified, including their species names. Phytochemical screening of each sample revealed the presence of glycoside in all of the tested species. However, no alkaloids, anthraquinones, and terpenoids were observed in those tested plant samples. Of the total 21 aquatic plants, 14 species contained flavonoids, 8 species contained phenol compound, and 10 species contained saponins. Among these species Donnax canniformis possessed good antioxidant activity, which correlated to its total phenolic and flavonoid contents. Our results would be beneficial for any future effort in the development of new herbal drugs derived from aquatic plants.

Perbanyakan Tanaman Pulai Pandak (Rauwolfia serpentina L.) dengan Teknik Kultur Jaringan Yunita, Rossa; Endang, Endang; Lestarai, Gati
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Due to over exploitation of its bark for medicinal herbs and made worse by problem in conventional breeding, Rauwolfia serpentina (Pulaipandak), has been considered rare and was currently reported to be an endangered species. Therefore, conservation measure is urgent to betaken. One of them is by in vitro propagation. In this research, in vitro propagation covers several activities, such as (1) shoot inductionwith the application of MS (Murashige and skoog) media enriched with ZPT 0.0; 0.1; 0.3 mg/l BAP combined with 0, 1, 2 mg/l 2ip, (2)shoot multiplication by using 0.0; 0.5; 1.0 mg/l BAP combined with 0.0; 0.1; 0.2 and 0.3 mg/l thidiazuron), (3) root induction IBA at theconcentration of 0.0; 0.5; 1.0; 1.5; 2.0; 2.5; 3.0 mg/l, and (4) acclimatization. The result showed that the best shoot induction for calli isthe in vitro stem by the application of MS + 0.3 mg/l BAP + 1 mg/l 2ip basic media. For shoot multiplication, the best media was MS + 0.5mg/l BAP + 0.1 mg/l ; while the best formula for root induction was MS + 1 mg/l IBA. The best media for plantlet acclimatization is compost+ soil mixture in 1:1 ratio.

PERBANYAKAN TANAMAN HIAS AIR TAWAR Alternanthera reinickii DAN Ludwigia sp SECARA IN VITRO GUNA MENDUKUNG AQUASCAPE Irawan, Ruhil; Yunita, Rossa; Isma N., Media Fitri; M., Benny Diyah
JURNAL LITBANG KOTA PEKALONGAN Vol 17 (2019)
Publisher : BAPPEDA Kota Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.704 KB)

Water plants are the hydrophytic plants that occupy the highest ecology in aquatic environments. Alternanthera reinickii and Ludwigia sp are freshwater ornamental plants that are in great deman d of aquascape lovers market. Plant tissue culture is a technique to grow the growing parts of plants, either in the form of cells, tissues or organs in aseptic conditions in vitro. The study was conducted on 15 January to 15 April 2018, at the Center for Biotechnology Research and Bogor Agricultural Genetic Resources. This research is done by using experiment method in laboratory. From the results of research on ornamental plants Alternanthera reinickii data obtained on the leaf paremeter giving regeneration substances grow the type of BAP with a concentration of 0.3 ml / l capable of producing the largest number of leaves. In shoot height parameters obtained the highest results using Kinetin treatment concentration 0.1 ml / l. In the parameter of shoot num ber of growth regulator substances BAP concentration of 0.5 ml / l resulted in the largest number of shoots. In the parameter of the root number of BAP treatment with the concentration of 0.3 ml / l shows the largest number of roots. In contrast to the ornamental plant Ludwigia sp BAP treatment of 0.1 ml / l concentration is able to produce the highest number of leaves In the parameters of shoot BAP treatment at low concentrations of 0.1 ml / l. on the parameters of BAP treatment buds concentration of 0.3 m l / l resulted in the largest number of shoots. In the parameter of the root number of BAP treatme nt, the concentration of 0.1 ml/l showed the highest number of roots. Keywords: Alternanthera reinickii, Ludwigia sp, Tissue culture, BAP, Kinetin

Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro Ariyanti, Febrina; Tumilisar, Christiani; Yunita, Rossa
Bioma Vol. 10 No. 1 (2014): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l. Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

Phytochemical analysis and antibacterial activity of Staurogyne sp. in fish disease bacteria Nugraha, Media Fitri Isma; Novita, Hessy; Rajamuddin, Muh Alias; Yunita, Rossa; Enggarini, Wening; Reflinur, Reflinur; Maharani, Fasya Hadaina; Elya, Berna
Torani Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

This study was aimed to identify the potential use of active compounds extracted from Staurogyne sp as an antibacterial agent to control disease-causing bacteria in fish. Staurogyne sp, an aquatic plant collected from Bantimurung, South Sulawesi were subjected to allelochemical compound. Plant extracts were tested to five bacteria including four types of bacterial pathogen, such as Aeromonas hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, and Flavobacterium columnare, and one bacterial sensing quoroum, Chromobacterium violaceum. Based on phytochemical analysis, Staurogyne’s extracts derived from both stem and root contained flavonoids, phenols, and antioxidants compounds with the concentration of 0.018 mgQE/g, 0.3471 mgGAE/g, and 1004,391 IC50 µg/mL, respectively. In addition, Staurogyne plant extracts derived from both their stem and root has also produced both saponins and glycosides compounds. Plant extracts derived from Staurogyne’s leaves revealed three major chemical compounds such as flavonoids (0.77 mgQE/g), phenol (0.0629 mgGAE/g), and glycosides (+). However, no antioxidants and saponin compounds were detected. Applying plant extracts as an antibacterial on five disease-causing bacteria causing diseases in aquaculture, such as Aeromonas Hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, Flavobacterium columnare, and Chromobacterium violaceum bacterials revealed that at concentration of 0.1 g the Staurogyne sp extracts did not influence all the bacterials growth. However, by application of plant extract at concentration of 0.2 g on the growth media,three out of 5 tested bacterials (Aeromonas hydrophilla, Edwardsiella ictaluri, and Streptococcus agalactiae) showed intermediate inhibition responses on bacterial growth. The growth of remaining two bacterials pathogen, Flavobacterium columnare and Chromobacterium violaceum at 0.2 g plant extract of Staurogyne sp was not affected. This study revealed that the use of active compound derived from Staurogyne sp would be potential to be used in inhibiting disease-causing bacterial in fish in future. However, optimum concentration of the plant extracts, in particular on the inhibition of the growth of disease-causing bacteria in fish is still needed to adjust.Key words: Antibacterial, aquatic plant, Staurogyne sp, fish disease

Study of aquatic plants and ecological- physics Tempe Lake, Sulawesi Selatan Nugraha, Media Fitri Isma; Julzarika, Atriyon; Radjamuddin, Alias; Reflinur, Reflinur; Yunita, Rossa; Enggarini, Wening; Novita, Hessy
Torani Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Aquatic plants are an indicator of the fertility of an aquatic region. The waters of Lake Tempe are the largest waters of the lake area in South Sulawesi. Lake Tempe is located in the western part of Wajo District, precisely in Tempe District, about 7 km from Sengkang City towards the banks of the Walanae River in southern Sulawesi. The area is about 13,000 ha with a maximum depth of 5.5 m and can reach more than 30,000 ha during floods, and during the dry season, the inundation area reaches only 1,000 ha with a maximum depth of 1 m, located above the continental and Australian and Asian plates. This lake is one of the tectonic lakes in Indonesia. Every year silting the lake occurs. The Tempe hydro vegetation and eco-physical research were carried out in October 2017. The purpose of this study was to record aquatic plant species that live in Tempe Lake and observe ecological changes and physical properties of Lake Tempe. Aquatic plants are expected to be able to filter lake water. The results obtained are physical conditions of sharp-smelling water, unpleasant taste, dark brown, and cloudy color. Chemical indicators of NH3-N waters (0.2976-0.0634), PO4-P (0.0172-0.0844) NO2-N (undetectable), NO3-N (1.7131-1.9335), Sulphate (27.761900 - 37.047620), DO (6.88-7.18) and pH (7.88-8.02). There are 14 species of aquatic plants found in these waters. The most dominant species is water hyacinth. In the case of Tempe lake water vegetation results in siltation of the lake area.Keywords: Aquatic plant, Biodiversity, Species, Tempe lake,

Co-Authors Ariyanti, Febrina Atriyon Julzarika Berna Elya Christiani Tumilisar, Christiani Endang Endang, Endang Endang G Lestari Endang Gati Lestari Gati Lestarai Irawan, Ruhil Isma N., Media Fitri Iswari S Dewi M., Benny Diyah Maharani, Fasya Hadaina Novita, Hessy Nugraha, Media Fitri Isma Radjamuddin, Alias Rajamuddin, Muh Alias Rajamuddin, Muh Alias L REFLINUR REFLINUR Wening Enggarini

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