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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Produksi Tanaman AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI IJOBI

Endang Gati Lestari

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar 3A, Bogor 16111

Author-ID : 306201

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Neuroscience

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Articles

INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.

MEKANISME TOLERANSI DAN METODE SELEKSI TUMBUHAN YANG TAHAN TERHADAP CEKAM AN KEKERINGAN Lestari, Endang Gati
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Water is the main parameter to determine whether the yield potential of a plant is obtained or not. Water deficit on the tissue causes the disruption of all chemical process in the plant metabolism resulted in the plant growth impediment.In order to acquire the drought tolerance variety, various efforts have been conducted. Among others, in addition to the selection and characterization of the available germ plasma along with its cross-breeding, the development of the drought tolerance plant is conducted through somaclonal varitype induction. In the cultivation of the drought tolerance plant, beside physiological mechanism and biochemistry related to the plant tolerant to drought, the procedure of the selective and optimal selection should be managed.Hence,the mastery of the optimum selection techniques, in a relatively short time, the new drought tolerance lines could be obtained.

PRODUKSI BIBIT KENCUR (Kaempferia galanga L.) MELALUI KULTUR JARINGAN Lestari, Endang Gati; Hutami, Sri
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

To increase domestic and international demand of Kencur (Kaempferia galanga L.) makes this plant potentially develop.Is traditionally used to keep the body warm, as analgetic and expectorant. In the attemp of providing adequately and qualitatively uniformed supply, in vitro experiment has been conducted at BB-Biogen (Indonesian Center for Agricultural Biotechnology and Genetik Resources Research and Development).The selected rhizomes was used as explant. The experiment was orthogonally arranged consisting of MS vitamin and B5, and BA ( 0, 3 and 5 mg/1) and thidiazuron 0,1 mg/1. This experiment comprised three activities, they were shoot initiation, shoot multiplication and acclimatization. The result showed that MS + BA 3 mg/1 + thidiazuron 0,2 mg/1 could induce shoot formation. From the applied media, it was shown that the addition of MS vitamin at the MS basic media and BA 3 and 5 mg/1 added with thidiazuron could result the most optimum shoot, leaves and roots and was not significantly different from the addition of B5 vitamin at basic media of MS + BA 3 and 5 mg/1, 6.9 shoot was averagely produced in this media. The shoot could generate such an adequate number of root that it could be directly acclimatized. The acclimatized plantlet in the green house uses the mixture of soil and manure with the ratio of 1:1 can optimally grow.

Antibacterial Activity of Green Meniran Extract (Phyllanthus niruri Linn) on The Growth of Salmonella typhimurium Nafisah, Aulia Erta; Octavia, Bernadetta; Lestari, Endang Gati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.215

Salmonella typhimuriumis a bacterium that causes digestive infections, gastroenteritis, and food poisoning, psickcaused byBacterial infection is a health problem in developing countries, including Indonesia. Green meniran (Phyllanthus niruri L.), is a herbaceous plant containing flavonoids, alkaloids, saponins, and tannins which have antibacterial activity which is expected to suppress the development of Salmonella sp. The aim of the study was to determine the ability of green meniran extract as an antibacterial to inhibit the growth of Salmonella typhimurium bacteria. The study used a completely randomized factorial design consisting of two factors, namely extract concentrations of 5%, 10%, 20%, 40%, 80%, equipped with a positive control (chloramphenicol) and negative control (aquades), as well as the age of the inoculum of the bacterial growth phase. Testing the antibacterial activity using the Kirby-Bauer disc diffusion method. The results showed that green meniran extract had antibacterial activity against the growth of Salmonella typhimurium bacteria. Concentrations of 5%, 10%, 20%, 40%, and 80% produced inhibition zone diameters of 6.7 mm, 8.5 mm, 9.9 mm, 12 mm, and 14.6 mm, respectively. The concentration of 80% green meniran extract is effective in inhibiting the growth of Salmonella typhimurium bacteria with an antibacterial effectiveness value of 58.95%.

Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.76

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability

Regenerasi Kedelai (Glycine max L. Merr) Hasil Iradiasi Sinar Gamma Nisa, Masayu Nessya Khoirun; Lestari, Endang Gati; Ashari, Sumeru
Produksi Tanaman Vol. 6 No. 12 (2018)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Produksi kedelai masih belum mencukupi kebutuhan masyarakat di Indonesia. Salah satu upaya meningkatkan produksi kedelai adalah dengan penggunaan varietas unggul dan ekstensifikasi pada lahan kering. Pemuliaan mutasi in vitro merupakan salah satu cara mendapatkan varietas unggul melalui perbaikan sifat yang diinginkan. Penelitian bertujuan untuk mengetahui media terbaik guna meregenerasi planlet kedelai hasil iradiasi sinar Gamma 4. Penelitian dilaksanakan pada bulan Februari hingga Agustus 2017 di laboratorium biologi sel dan jaringan dan rumah kaca Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Bogor. Penelitian menggunakan Rancangan Acak Lengkap dengan perlakuan iradiasi sinar gamma 4 Gray. Dari hasil analisis data kombinasi media MS+BA 0,5 mg/l+Kin 0,1 mg/l merupakan media regenerasi terbaik. Terdapatpengaruh dari perlakuan iradiasi sinar Gamma 4 Gray terhadap penurunan pertumbuhan kedelai varietas Grobogan, Wilis dan Dering 1. Serta didapatkan 14 planlet kedelai yang berhasil diaklimatisasi.

Identifikasi Somaklon Padi Gajahmungkur, Towuti dan IR 64 Tahan Kekeringan Menggunakan Polyethylene Glycol Lestari, Endang Gati; Mariska, Ika
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 2 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The drought stress tolerant and high yielding rice is needed in upland rice system. The changing global climate makes dry season longer, resulting in the reduction of rice production. There should be an effort to introduce new variety of high-yielding and drought tolerant rice. In this attempt, research was conducted to improve the genetic of Indica rice, particularly Gajahmungkur, Towuti and IR 64 varieties in order to find the somaclones with the characteristics above. As an approach, gamma-ray mutative induction was applied to be followed by selection in PEG. The regenerated shoot from the irradiated callus was then selected and acclimatisized in the greenhouse to obtain eighty three somaclones from the three varieties. PEG (molecular weight 6000) was applied to obtain the drought-tolerant somaclone. PEG was a selective agent used by which populations could be selected in a short time. Treatment with 20% PEG (equals to osmotic potential 1.2 Mpa) on the rice produced 16 somaclones from Gajahmungkur, 12 from Towuti and 18 from IR 64 putatively drought tolerant. Key words : Oryza sativa, drought tolerance, PEG

Inisiasi Tunas Ganda Tanaman Manggis Malinau melalui Kultur In Vitro untuk Perbanyakan Klonal Lestari, Endang Gati; Suhartanto, M. Rahmad; Kurniawati, Ani; Rahayu, Suci
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Mangosteen (Garcinia mangostana L.) is one of the most promising tropical fruits for export. The major constraint toincrease fruit production of the spesies is the long juvenile period. Seedless, sweet and juicy variety of mangosteen had beenfound in Malinau. In vitro propagation technique offers possibility to produce sufficient number of seedlings any time. Thisresearch was aimed at obtaining the appropriate media formula to enhance shoot proliferation. This research consisted ofshoot induction and multiplication and shoot elongation. The materials were the fresh mangosteen seeds from the Malinaumangosteen trees. The explant used in the trial was seeds which were divided into four slices. The use of 8 to 16 mg BA L-1combined with 0.2 mg thidiazuron L-1 resulted in the best shoot induction of 52 shoot buds per explant at the 6th week afterplanting with the mean height of 0.3 cm. Upon subculturing in to the similar media, the number of shoot tends to increase.For multiplication, low concentration of BA (2 to 4 mg L-1) and thidiazuron 0.05 mg L-1 were applied to increase the numbersof shoots. The total shoot number obtained in the media with 0.05 thidiazuron without BA was 11.25 and in the media with 2mg BA L-1 + 0.05 mg thidiazuron L-1 was 8.7 shoot explant-1. The result showed that the best media for shoot elongation wasMS + 1 mg BA L-1 + 2 mg kinetin L-1. The length of the shoots were in the range of 0.5-0.8 cm.Keywords: BA, Garcinia mangostana, in vitro culture, shoot multiplication, thidiazuron

Penampilan Galur Harapan Mutan Dihaploid Padi Tipe Baru di Sulawesi Selatan Dewi, Iswari Saraswati; Lestari, Endang Gati; ,, Chaerani; Yunita, Rossa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTSouth Sulawesi is known as one of national rice production centers. However, average productivity of rice varieties planted in that area (4.43 ton ha-1) is lower than those of rice productivity in Java (5.25 ton ha-1). The aims of this research were to evaluate agronomic characters and adaptation of 7 dihaploid mutant advanced lines of new plant type (DH-NPT) of rice at several locations in South Sulawesi. The research was conducted in 2012 at Maros, Gowa, Barru, and Pangkep. The experiments were conducted in randomized complete block design with 3 replications nested in locations. Treatment consisted of 7 DH-NPT of rice, i.e., BIO-MF115, BIO-MF116, BIO-MF125, BIO-MF130, BIO-MF133, BIO-MF151, BIO-MF153, and control varieties i.e., Fatmawati, Ciherang, and Inpari13. The results indicated that in general the lines had medium height (102.77-110.23 cm), moderate productive tiller (9-16 tiller per hill), moderate days to flower (50%), i.e., 73-76 days after sowing (DAS), earlier days to harvest (103-110 DAS), moderate panicle length (28.35-29.31 cm), large number of grain per panicle (> 250 grains) with moderate panicle fertility (63-70%), moderate 1,000 grain weight, i.e., 26.51-27.75 g, and high yield (7.51-8.09 ton ha-1). Four lines, i.e., BIO-MF116, BIO-MF130, BIO-MF151, and BIO-MF153 were stable and had wide adaptability. Other lines, i.e., BIO-MF125 and BIO-MF133 were sensitive to environmental changes, therefore they were classified as specifically adapted to favorable environment; while BIO-MF115 was not sensitive to environmental changes, and therefore it was adapted to non-favorable environment.Keywords: adaptation, agronomic characters, rice mutant

Co-Authors Ani Kurniawati Bernadetta Octavia Chaerani ,, Chaerani Ika Mariska Iswari Saraswati Dewi Mohamad Rahmad Suhartanto Muhamad Syukur Nafisah, Aulia Erta Nisa, Masayu Nessya Khoirun RAGAPADMI PURNAMANINGSIH ROHIM FIRDAUS Rosa Yunita ROSSA YUNITA Sri Hutami Suci Rahayu Sumeru Ashari Syarifah Iis Aisyah

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