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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 26 Documents
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Search results for , issue "Vol 9, No 1 (2008)" : 26 Documents clear
MENGESTIMASI NILAI KERUSAKAN TUMBUHAN INANG AKIVAT PEMARASITAN BENALU Sunaryo Sunaryo
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.810

Abstract

Benalu merupakan kelompok tumbuhan parasit yang termasuk ke dalam suku (famili) Loranthaceae.Beberapa pengarang membagi suku ini menjadi duaanak suku, yaitu Loranthoideae dan Viscoideae. Tetapi beberapa pengarang lain memisahkannya menjadi dua suku tersendiri, yaitu Loranthaceae dan Viscaceae[Barlow BA. 1967. Loranthaceae. In: Flora MalesianaSeries I, vol. 13,209-401. C Kalkman, DWKirkup, HPNootebom, PF Stevens and WJJO de Wilde (Eds.). Rijksherbarium/Hortus Botanicus, The Netherlands]. Suku Loranthaceae memiliki tidak kurang dari 940 jenis(spesies), yang termasuk dalam 70 marga [Anonymous2006. Taxonomy of Loranthaceae. http://www.parasiticplants/1 .htmll. Keseluruhan jenisnya bersifat hemiparasit/ parasit fakultatif, karena di dalamsiklus hidupnya dapat melakukan proses fotosintesamelalui keberadaan pigmen hijau daun.
PEMBUATAN STARTER UNTUK EKSTRAKSI MINYAK KELAPA MURNI MENGGUNAKAN MIKROBA AMILOLITIK Elidar Naiola
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.801

Abstract

The thirteen isolates of amylolitic microbes had been tested their ability to extract the oil from " coconut milk" and nine of them could break the emulsion and separated the oil from the water and protein. The aim of this study was to find a starter that can be used for producing the coconut oil by using molase and "gula aer" gewang (Corypha utan Lamk.) palm sugar as the substrates.The result suggest that by using the isolates (ferm. 1 and ferm.2), "gula aer"gewang can be used as a substrate without supplemented by organic nitrogen. The starter prepared with isolate ferm. 1 containing cells of microbe about 10.2 x 10 cell/ml and prepared with ferm.2 about 9.0 - 10.2 x 10 cell/ml. After 4 weeks the amount of the cells decreased to 0.98 x 10 cell/ml and 0.90 x 10cell/ml, respectively, The amount of microbes were stable until 12 weeks.The starter conducted the fermentation processes at 40°C for 16 hours and produced the coconut oil. The extracted oil content about 85% saturated fatty acids and 42% of them was lauric acid. Another chemical component of the extracted oil were Iodine numbers, peroxide numbers and free fatty acid (FFA), they were 5.98%, 2.51 Meq/kg and 0.41%, respectively.
KARAKTERISASI 17 FAMILI IKAN NILA {Oreochromis niloticus) GENERASI KE TIGA (G-3) BERDASARKAN METODE TRUSS MORFOMETRIKS Nuryadi Nuryadi; Otong Zenal Ariiin; Rudhy Gustiano; Mulyasari Mulyasari
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.806

Abstract

Objective of the study is to elucidate phenotype characters of nile tilapia (Oreochromis niloticus) of the third generation (G3)resulted from the selection breeding program in the Research Institute for Freshwater Aquaculture Bogor. Phenotypes of seventeen nile tilapia families, were observed using truss morphometric methods. The results showed that the composition average of standard lenght has low coefficient variation mean (10.42%) as well as coefficient variation of the truss measured characters (4.3- 13.3%). The highest index similarity of boddy shape within family was found in family 5 (79.3%), in contrast the lowest was in family 12 (32.3%). For index similarity between families, the highest score was in between family 12 and 17 (22.6).There were 8 characters enabled to differentiate the 17 families observed. They were Al, A3, A5, B2, C3, C5, C5, and D4. Cluster analyses recognized into 4 groups based on the phenotypic distance
RETRANSFORMATION AND EXPRESSION OF RECOMBINANT VIRAL PROTEIN OF JEMBRANA JSU AND JTat (JSU AND JTat) IN pGEX SYSTEM [Retransformasi dan Ekspresi Protein Virus Rekombinan JSU dan JTat Penyakit Jembrana dalam Sistem pGex] Endang T Margawati; Andi Utama; Indriawati Indriawati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.802

Abstract

Genom virus penyakit Jembrana setidaknya memiliki 3 gen besar yang menyandi protein dan beberapa di antaranya diperlukan untuk replikasi virus. Protein JSU dan JTat diduga dapat menginduksi kekebalan yang protektif pada sapi Bali terhadap penyakit Jembrana sehingga keduanya sangat berpotensi untuk dipakai sebagai vaksin rekombinan. Penelitian ini dirancang untuk meretransformasi protein rekombinan JSU dan JTat ke dalam Escherichia coli menggunakan sistem pGEX. Konstruk JSU dan JTat dalam pGEX dikoleksi plasmidnya dengan metode miniprep dan kemudian diretranformasikan ke dalam E. coli strain BL21 dan DH5a. JSU dan JTat hasil retransformasi diekspresikan pada medium LB untuk skala produksi kecil dengan sistem pGEX. Hasil penelitian ini meminjukkan bahwa kedua JSU dan JTat hasil retransformasi ke dalam E. coli strain BL21 terlihat tumbuh lebih baik pada medium LB jika dibandingkan retransformasi ke dalam E. coli strain DH5a. Hasil retransformasi JSU dan JTat dikarakterisasi dan diidentifikasi dengan Western blotting dan tampak menunjukkan ukuran protein yang benar, yaitu protein rekombinan JSU berukuran 60kDa dan JTat berukuran 36,7kDa. Protein rekombinan JSU muncul dengan pita tunggal dan lebih jelas jika dibandingkan dengan protein JTat. Konsentrasi protein JSU sedikit lebih rendah (1,883 mg ml ) jika dibandingkan dengan JTat (l,981mg ml').Penelitian ini menunjukkan bahwa JSU pGEX masih tersimpan dan diekspresikan dengan baik, sementara JTat mungkin perlu dilakukan perakitan ulang untuk memantapkan ekspresinya.
INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Rossa Yunita; Endang Gati Lestari
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

Abstract

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.
KERUSAKAN DINDING SEL Escherichia coli OLEH MINYAK ATSIRI TEMU KUNCI (Kaempferiapandurata) Miksusanti Miksusanti; Betty Sri Laksmi Jennie; Bambang Ponco; Gatot Trimulyadi
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.775

Abstract

Antibacterial activity of temu kunci (Kaempferiapandurata) essential oil against Escherichia coli Kl.l was analyzed. Activity of antibacterial essential oil was analyzed through it's ability to leak the Escherichia coli Kl.l cell wall and altering it.Minimum Inhibitory Concentration (MIC) of temu kunci essential oil is 0.11% (v/v). Further studies were conducted using the concentration of 1 MIC and 2 MIC.Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry(UV).Alteration of cell wall was analyzed with scanning electron microscopy (SEM).The optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm.The result showed that K. pandurata essential oil could leak the inorganic ion Ca 17-53%, and K* 9-43% from the bacteria and alter the cell wall of the bacteria.

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