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BERITA BIOLOGI
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Articles 2,125 Documents
PENGARUH PENAMBAHAN GLISEROL PADA PAKAN TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN NILA (Oreochromis niloticus) Lusi Herawati Suryaningrum; Mulyasari Mulyasari; Reza Samsudin
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2833

Abstract

The aim of this research was to determine the optimum percentage of glycerol addition into the diets of  Nile Tilapia (Oreochromis niloticus) on growth performance, feed convertion ratio, and survival rate. The research was conducted using 15 aquaria with the size of 60 x 50 x 45 cm3, each containing 25 O. niloticus with average initial weight of 4.63 ± 0.15 g. The experiment was conducted using a complete random design with five treatments and three replications. Isonitrogenous (31%) and isocaloric (17 MJ.kg-1) diets were provided for 60 days of rearing period. Five diets were formulated with glycerol content of 0% (G0); 5% (G5); 10% (G10); 15% (G15), and 20% (G20). Fish were fed twice daily with experimental diet at satiation level. The result showed that addition of 10% glycerol in the diet (G10) presented the highest values for weight gain (105.85 ± 0.40%), specific growth rate (3.44 ± 0.01%), protein retention (27.75 ± 0.02%), protein efficiency ratio (2.53 ± 0.15%), lipid retention (60.44 ± 0.03%), and feed convertion ratio (1.59 ± 0.01%) (P < 0.05). Survival rate was not affected by dietary containing glycerol (P > 0.05). Therefore, addition of 10% glycerol into the diet gave a significant increase on growth performance and feed convertion ratio without adverse effect on survival rate of Nile Tilapia.  
MENYELARASKAN ATURAN HPH Toto Iman Suparto
BERITA BIOLOGI Vol 6, No 2 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i2.1479

Abstract

Ada tiga hal penting yang tercakup dalam makalah berjudul "Kebijakan, Otonomi Daerah dan Era Pasar Bebas Dalam Pemanfaatan Hasil Hutan"yang ditulis oleh Aminuddin Ponulele dan A.Ngaloken Gintings.
Di Sela-Sela Laboratorium dan Plot Eksperimen MENGENAL HPLC: PERANANNYA DALAM ANALISA DAN PROSES ISOLASI BAHAN KIMIA ALAM Tri Murningsih; Chairul Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1161

Abstract

The development of method to explore natural chemical product initially from plants were started from the beginning and generously from time to time from the conventional one's until sophisticated equipments.The value of plants as food,cosmetic and medicines resources needs specific instruments and methods for determining the quantity as well as quality of the materials in plants.This paper describe in detail one of the sophisticated equipments commonly used for analyzing and isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).
HASIL DAN STABILITAS HASIL BIJI KEDELAI {Glycine max (L.) Merr.} GALUR HARAPAN DI LAHAN SAWAH Ayda Krisnawati; M Muchlish Adie; GWA Susanto
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.524

Abstract

Soybean {Glycine max (L.) Merr.} varieties with consistently high yield productivity across environments are expected to maintain its production level per area.The objectives of this experiment are to determine the magnitude of G × E interaction and to identify the stability of eight soybean promising lines across locations. Materials consists of eight soybean promising lines (G100H/SHRW-60-38, SHRW-60/G100H-73, SHRW-60/G100H-68, SHRW-60/G100H-66, G100H/SHRW-34, SHRW-60/G100H-5, SHRW-60/G100H-70 and SHRW-60/G 100 H-75) and two check varieties (Kaba and Wilis). The experiments were done in 16 locations (Lampung Tengah, Yogyakarta, Ngawi, Mojokerto, Pasuruan, Malang, Banyuwangi and Lombok Barat, two locations each) during the period of 2009 to 2011. A randomized completely block design with four replicates was used in each location. AMMI analysis (Additive Main Effects and Multiplicative Interaction) was applied to assess the yield stability of those 10 genotypes, and then interpreted in biplot graphic of seed yield for principal components 1 (IPCA1) with the principal component 2 (IPCA2). Seed yield of the 10 soybean lines ranged from 2.63-3.02 t/ha, with 2.81 t/ha in average. The highest yield was obtained by G6 (SHRW-60/G100H-5), whereas G3 (SHRW-60/G100H-68) had the lowest seed yield.The combined analysis showed that lines, locations, and the interaction of lines and locations (G × L) were significantly different for seed yield.The first four Interaction Principal Component Axes (IPCA1, IPCA2, IPCA3 and IPCA4) were significant and accounted for 85.1% of the total GEI. Lines of G100H/SHRW-60-38 (G1), SHRW-60/G100H-66 (G4) and SHRW-60/G100H-5 (G6) were stable and high yielding, and therefore they are proposed to be released as new varieties. The results of this study also suggested that Kaba and Willis were used as specific-check varieties, due to its site-specific adaptability.
ANALISIS VEGETASI POHON DI HUTAN HUJAN TROPIK HARAPAN, JAMBI Muhammad Mansur; Teguh Triono; Ismail Ismail; Setyawan Warsono Adi; Enu Wahyu; Gofar Ismail
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1970

Abstract

Vegetation analysis of trees in Harapan Rainforest, PT.REKI, Jambi, was conducted on October-November 2009. Two 50m x 100m (0,5 ha) plots were established to obtain primary data for analysis. Plot one was made in the area of Medium Secondary Forest and plot two in the area of High Secondary Forest. Results shows that, the number of tree species were recorded from the two plots was 205 species from 550 individual trees with a total Basal Area (BA)= 64.75 m2. Plot one has 123 species from 284 individuals with BA = 10.82 m2 and Diversity Index (H')= -1.87. Bellucia axinanthera was recorded as the dominant species with Importance Value (IV)= 37.21%. Meanwhile, plot two has 126 species from 266 individuals with BA= 53.93 m2 and (H')= -1.97. Shorea acuminata was recorded as the dominant species with IV= 12.93%. Plot two (High Secunder Forest) has higher diversity of tree species than plot one (Medium Secunder Forest).The structure and composition of vegetation between plot one and plot two is different with similarity index for the two plots is only 21.46%.
PERTUMBUHAN BEBERAPA JENIS LEGUMINOSA TUMBUH-CEPAT DI LAPANGAN SETELAH SEMABSfYA DIINOKULASI DENGAN RHIZOBIUM S. DANIMIHARDJA; SAEFUDIN SAEFUDIN; F. SYARIF; N. SETYOWATMNDARTO
BERITA BIOLOGI Vol 3, No 8 (1988)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i8.1304

Abstract

S. DANIMIHARDJA,SAEFUDIN,F. SYARIF & N.SETYOWATMNDARTO. 1987. The growthof some fast-growing legume species in the field after seedling inoculation with Rhizobium.Berita Biologi 3(8): 377 - 381.Eleven species of fast growing legumes were grown in the field after their root seedlings were inoculated with two selected strains of Rhizobium sp.The results indicated that Acacia villosa was the fastest growing species (324.8 cm in height and 28.35 mm in diameter after 13 months of planting).The slowest one was Albizia procera (86.20 cm and 10.45 mm).The statistical analysis indicated that between species there were highly significant differences either in height or in diameter of the plants. Between treatments there was significant difference in diameter, but not in height.The interaction between treatments and species was highly significant difference in diameter, but not significant one in height. After 13 months of planting 6 species had already produced pods,namely Acacia villosa,Albizia lebbeck,Bauhinia acurninata, B. purpurea, Caesalpinia pulcherrima and 7 C.sappan.The death rates were ranging from 19% (in Albizia chinensis),30%(in A. falcatariaj, 46% (in A. procera),47% (in A. lebbeck), until 50% (in Acacia auriculiformis).
RETRANSFORMATION AND EXPRESSION OF RECOMBINANT VIRAL PROTEIN OF JEMBRANA JSU AND JTat (JSU AND JTat) IN pGEX SYSTEM [Retransformasi dan Ekspresi Protein Virus Rekombinan JSU dan JTat Penyakit Jembrana dalam Sistem pGex] Endang T Margawati; Andi Utama; Indriawati Indriawati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.802

Abstract

Genom virus penyakit Jembrana setidaknya memiliki 3 gen besar yang menyandi protein dan beberapa di antaranya diperlukan untuk replikasi virus. Protein JSU dan JTat diduga dapat menginduksi kekebalan yang protektif pada sapi Bali terhadap penyakit Jembrana sehingga keduanya sangat berpotensi untuk dipakai sebagai vaksin rekombinan. Penelitian ini dirancang untuk meretransformasi protein rekombinan JSU dan JTat ke dalam Escherichia coli menggunakan sistem pGEX. Konstruk JSU dan JTat dalam pGEX dikoleksi plasmidnya dengan metode miniprep dan kemudian diretranformasikan ke dalam E. coli strain BL21 dan DH5a. JSU dan JTat hasil retransformasi diekspresikan pada medium LB untuk skala produksi kecil dengan sistem pGEX. Hasil penelitian ini meminjukkan bahwa kedua JSU dan JTat hasil retransformasi ke dalam E. coli strain BL21 terlihat tumbuh lebih baik pada medium LB jika dibandingkan retransformasi ke dalam E. coli strain DH5a. Hasil retransformasi JSU dan JTat dikarakterisasi dan diidentifikasi dengan Western blotting dan tampak menunjukkan ukuran protein yang benar, yaitu protein rekombinan JSU berukuran 60kDa dan JTat berukuran 36,7kDa. Protein rekombinan JSU muncul dengan pita tunggal dan lebih jelas jika dibandingkan dengan protein JTat. Konsentrasi protein JSU sedikit lebih rendah (1,883 mg ml ) jika dibandingkan dengan JTat (l,981mg ml').Penelitian ini menunjukkan bahwa JSU pGEX masih tersimpan dan diekspresikan dengan baik, sementara JTat mungkin perlu dilakukan perakitan ulang untuk memantapkan ekspresinya.
DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Atit Kanti; I Nyoman Sumerta
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2215

Abstract

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.
NOTES ON THE MOLLUSCAN FAUNA OF PULAU PARI Subagjo Soemodihardjo
BERITA BIOLOGI Vol 1, No 4 (1974)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v1i4.1444

Abstract

Among the soores of islands that make up "Pulau Seribu",Pulau Pari is ecologically interesting. It is composed of five small islands united by a common., distantly located, outer reef.Collectively they offer a long beach line easily within our reach.The vast intertidal area, the uneven bottom topography and the nature of the bottom, give rise to different kind of habitats from which we may expect different biotic populations.
EKOLOGI HUTAN GAMBUT DITAMAN NASIONAL TANJUNG PUTING, KALIMANTAN TENGAH Edi Mirmanto; Ruddy Polosakan; Herwint Simbolon
BERITA BIOLOGI Vol 5, No 3 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i3.1116

Abstract

Ecological studies of peat-swamp forest was conducted in the Tanjung Puting National Park,Central Kalimantan. One-hectare permanent plot has been establishes in order to describe the forest structure and floristic composition. Within 1-ha plot 141 species of tree (dbh >10 cm) and saplings (dbh 5-10 cm) belongs to 84 genera and 43 families recorded. Glutta wallichii, Neoscortechinia philippinensis and Gonystyllus bancanus were dominant tree species, whereas Baccaurea racemosa was very dominant for sapling stage.Tree's density 2 2 was 728 /ha with basal area of 43.01 m /ha whereas density of sapling was 904 /ha with basal area of 3.8 m /ha. Most (30.7 %) of trees with dbh 10 - 30 cm and only 2.23 % of them reach up to dbh > 50 cm. There are three canopy layers that are I-layer was 26.8-33.6 m,II-layer was 17.8-26.8 m and Ill-layer was 9.8-17.6 m high.

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