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BERITA BIOLOGI
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Articles 2,125 Documents
PENGARUH Mangan (Mn) TERHADAP OKSIDASI KROMIUM TRIVALEN DAN PERTUMBUHAN Tagetes sp. Margareta Prameswati; Sri Kasmiyati; Sucahyo Sucahyo
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4190

Abstract

Chromium can be found in 2 oxidation states, trivalent chromium (Cr3+) and hexavalent chromium (Cr6+) in environment. Trivalent chromium can be oxidized by manganese oxide to hexavalent chromium (Cr6+). The aim of the study was to determine the effect of Mn on the oxidation of Cr³⁺and plant growth of Tagetes. The study was conducted experimentally using a completely randomized design. The Tagetes plants grown in media with 2 treatment factors, namely Cr3+ (CrCl3) with 3 concentrations of 0, 100 and 500 mg/l) and Mn (MnSO4) with 4 concentrations of 0, 1, 5 and 10 mg/l. The plant growth and Cr6+ content in roots and shoots were measured to determine the effect and to detect the occurrence of Cr3+ oxidation. The addition of Mn in the media of Tagetes containing Cr3+ increased the Cr3+ oxidation process, indicated by the detection of Cr6+ in the roots and shoots of Tagetes. The highest Cr6+ content was found in the roots and shoots of Tagetes sp. treated with Cr3+ 500 mg/l and Mn 10 mg/l at 0.059 and 0.035 g/l, respectively. The interaction of Cr3+ and Mn treatments significantly affected the growth of Tagetes plants. Cr3+ and Mn at high concentrations increased plant height, root length, root and shoot dry weight. The interaction of Cr3+ and Mn at high concentrations inhibited the increase in the number of leaves of Tagetes.
DIVERSITY OF NEPENTHES SPECIES IN NORTH SUMATRA PROVINCE Mansur Halik; Andi Salamah; Edi Mirmanto
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4389

Abstract

Sumatra is the second island after Borneo, which has the highest diversity of Nepenthes species, spread from the lowlands to mountain forests. There are 38 species of Nepenthes recorded on Sumatra Island, and 33 of them are endemic. The study was conducted in 2019 and 2022 to know the diversity of Nepenthes in North Sumatra Province. The results showed that 22 species of Nepenthes were recorded in North Sumatra Province, consisting of 12 highland species, 3 midland species and 7 lowland species. N. tobaica is a highland species with a very wide distribution, spread over eight regencies in North Sumatra with a very abundant population, while N. sumatrana, N. rigidifolia, N. jamban, N. lingulata, and N. naga have limited distribution with a very small population and are potentially extinct.
Efektivitas Antifungi Senyawa Non Atsiri Dari Ekstrak Curcuma aeruginosa Terhadap Colletotrichum capsici Pada Buah Cabai Merah Anella Retna Kumala Sari; Arrohmatus Syafaqoh Li'aini
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4059

Abstract

ABSTRAK            Penyakit antraknosa yang disebabkan oleh Colletotrichum capsici masih menjadi penyakit utama pada buah cabai merah. Selama ini, penggunaan Curcuma aeruginosa sebagai antimikroba patogen penyebab penyakit pada manusia lebih popular daripada sebagai antimikroba fitopatogen. Rimpang Curcuma diketahui mengandung senyawa atsiri dan non atsiri. Potensi senyawa atsiri Curcuma sebagai antimikroba telah banyak dilaporkan, namun masih sangat terbatas untuk senyawa non atsirinya.  Penelitian ini bertujuan untuk mengetahui efektivitas antifungi senyawa non atsiri dari ekstrak C. aeruginosa terhadap C. capsici. pada buah cabai merah. Senyawa non atsiri didapatkan dengan merendam rimpang C. aeruginosa menggunakan pelarut metanol kemudian didistilasi menggunakan rotary vacuum evaporator dan diidentifikasi menggunakan HPLC. Efektivitas antifungi senyawa non atsiri dari ekstrak rimpang C. aeruginosa diuji secara in vitro dan in vivo menggunakan Rancangan Acak Lengkap dengan 3 kali ulangan. Perlakuan yang diujikan yaitu konsentrasi senyawa non atsiri terdiri dari 0 ppm (kontrol) ; 4 ppm ; 6 ppm ; 8 ppm ; 10 ppm dan 12 ppm. Senyawa non atsiri dari ekstrak rimpang C. aeruginosa sangat efektif menghambat pertumbuhan C. capsici bahkan konsentrasi 12 ppm menunjukkan persentase penghambatan antraknosa hingga 100%. Hasil identifikasi HPLC menunjukkan bahwa senyawa non atsiri dari ekstrak rimpang C. aeruginosa mengandung kelompok Curcuminoid yang berpotensi sebagai fungisida nabati.Kata kunci : Senyawa Non Atsiri ; C. aeruginosa ; Antraknosa ; Cabai Merah   ABSTRACTAnthracnose disease caused by Colletotrichum capsici is still as major disease in chilli pepper fruit. During this time, utilization of C. aeruginosa as antimicrobial of pathogen caused disease to human is more popular than to crops. Curcuma has been known containing essential and non essential compound. Potential of essential compound from Curcuma as antipathogen has been reported widely, nevertheless it is still limited known for non essential compound as antipathogen. This research aimed to understand the antifungi effectivity of non essential compound from C. aeruginosa extract to C. capsici on chilli pepper fruit. Non essential compound was obtained by soaking C. aeruginosa rhizome into methanol solvent then distilated using rotary vacuum evaporator and identified with HPLC instrument. Antifungi effectivity of non essential compound from C. aeruginosa rhizome extract was experimented by using Completely Randomized Design with 3 replications. Treatments tested were various concentrations of non essential compound namely 0 (control) ; 4 ; 6 ; 8 ; 10 and 12 ppm. Non essential compound from C. aeruginosa rhizome extract highly effective to inhibit growth of C. capsici even 12 ppm concentration showed inhibition percentage up to 100%. HPLC identification result showed non essential compound from C. aeruginosa rhizome extract contains Curcuminoid group which potential to be used as organic fungicide.Keywords : Non Essential Compound ; C. aeruginosa ; Anthracnose ; Chilli Pepper Fruit
Response of Local Cowpea Varieties Southwest Maluku to Drought Stress on Germination Phase Ritha Lusian Karuwal; Hermalina Sinay
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4222

Abstract

Drought stress is one of the agricultural problem, especially in dry land. Respone to drought stress of local cowpea varieties Southwest Maluku on germination phase had not been conducted. The aim of this study was to evaluate response of local cowpea varities to drought stress on germination phase. The research was using seven of local varieties (KM1, KM3, KM4, KM6, KM7, KM8, KM9) from Kisar island and three cultivars from ILETRI Malang. Treatment of drought stress were consist of watering period every twice days (P0) and five day (P1) was replicated three times. Sprout number, final germination percent, sprout length, sprout weight, vigor index and prolin content in root were observed. Data were analyzed using ANOVA, followed by DMRT at 5%. The results showed that treatment of every five day inhibit germination at all varieties. KM1, KM4, KM6 varieties has the highest value on all parameter. There are positive correlation which significant between of the observation parameter. Principal component analysis to clustering in two main cluster. Early selection of drought tolerance at germination phase very need to breeding plant in effort of the suistainble development and utilization.
AKTIVITAS DAN KARAKTERISASI MIKOSIN ANTI Zygosaccharomyces rouxii FNCC 3006 DARI ISOLAT KHAMIR OSMOFILIK ASAL NEKTAR BUNGA DAN MADU Sovia Nur Janah; Miftahul Ilmi
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4095

Abstract

Makanan berkadar gula tinggi (40-70%) dapat terkontaminasi oleh Zygosaccharomyces rouxii yang toleran terhadap tekanan osmotik, asam, alkohol, serta tahan terhadap bahan preservatif makanan. Salah satu cara menghambat pertumbuhan Z. rouxii adalah dengan memberikan mikosin anti Z. rouxii. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas dan karakterisasi mikosin anti Z. rouxii dari isolat khamir osmofilik. Penapisan aktivitas antagonistik dari isolat khamir osmofilik dilakukan dengan metode Colony Picking dan penapisan aktivitas ekstrak kasar dilakukan dengan Disk Diffusion Assay. Zona jernih yang terbentuk diukur untuk menentukan aktivitasnya dengan menggunakan indeks penghambatan (IP). Setelah itu dilakukan pengukuran berat kering sel dan karakterisasi mikosin menggunakan SDS PAGE. Hasil penelitian menunjukkan terdapat 10 isolat khamir osmofilik yang menghasilkan aktivitas antagonistik terhadap Z. rouxii yaitu isolat AP 1, PS 1.1, LW 1.1, BK 3.2, MR 3.2, AP 3, KS 1, dan TL 2.2 yang diisolasi dari madu, isolat BL 8.21 dan BL 8.22 yang diisolasi dari nektar bunga. Aktivitas ekstrak kasar mikosin anti Z. rouxii paling tinggi yaitu isolat LW 1.1 dengan nilai IP 1,28.  Isolat paling efektif untuk menghambat pertumbuhan dari Z. rouxii adalah isolat LW 1.1, diperoleh nilai perbandingan zona hambat dengan berat kering yaitu 442,099. Isolat LW 1.1 menghasilkan anti-Z. rouxii dengan berat molekul antara 12,88 kDa sampai 72,92 kDa. Disimpulkan bahwa isolat LW 1.1 dapat dijadikan sumber penghasil mikosin anti-Z. rouxii yang dapat dimanfaatkan sebagai agen biokontrol dalam produk makanan.

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