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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 32 Documents
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Search results for , issue "Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA" : 32 Documents clear
ANALISIS SEKUEN DNA MITOKONDRIA CYTOCHROME OXIDASE I (COI) MTDNA PADA KUKANG INDONESIA (NYCTICEBUS SPP) SEBAGAI PENANDA GUNA PENGEMBANGAN IDENTIFIKASI SPESIES Wirdateti, Wirdateti; Indriana, Eka; Handayani, Handayani
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2322

Abstract

Slow lories is small size primates of the genus Nycticebus that are in endangered status. Habitat pressure andhigh trade as pet animals has made the population decline both quantitatively and qualitatively, therefore it isnecessary to save the slow lories through conservation action. Morphologically, the individuals are difficult tobe distinguished among the species, therefore making much harder in identification for conservation purposessuch as from confiscated individuals. Therefore molecular marker is needed through mitochondrial DNA usingCOI gene sequences analysis is needed for identification. This study used 24 samples of slow lories consistedof 2 from Kalimantan, 9 from Java and 13 from Sumatra. The result indicated that in the 660 bp there were 62variable sites with 15 haplotypes: 4 haplotypes of N. javanicus, 9 haplotypes N. coucang, and 2 haplotypes N.menagensis. Base on nucleotide variations at specific sites, it was formed haplotype differences among species.The average genetic distance between species showed that Kalimanatan population were closer to thepopulation of Sumatra (d= 0.042 ± 0.006) compared with Java population (d= 0.059 ± 0.009), whereas thegenetic distance within population showed the population of Java had low diversity (d= 0.002) with a meannucleotide differences 2.6. Thus Jawan slow loris (N. javanicus) should be conserved.Keywords: Nycticebus spp., molecular, COI, conservation, marker
PEMBENTUKAN DAN PENAMPILAN PISANG REJANG HIBRID TRIPLOID HASIL PERSILANGAN PISANG REJANG MIXOPLOID DENGAN PISANG REJANG DIPLOID Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Abstract

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids
OPSI PENDANAAN BIODIVERSITY DI INDONESIA Haryanto, Joko Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2318

Abstract

The contribution of biodiversity to economic development, social and ecological in Indonesia today and thefuture are already being felt very real. Unfortunately, as well as economic development approach to the greeneconomy that appears on the answers rampant deforestation and forest degradation, biodiversity atmospheretoday in Indonesia is under pressure and decrease impact on the species and environmental balance problemsand poverty. Besides the problem of public funding also be other problems. In this case, government role to bevery significant through the Transfer to the Regions. By using the methodology analysis desk study, it can berecommended that General Allocation Fund (DAU) is the main policy priority for funding biodiversity, throughthe addition of indicators of the Fiscal Needs (KbF). Other policy options are DAK mechanism throughimprovement criteria.Keywords: Biodiveristy, General Allocation Fund (DAU), Specific Allocation Fund (DAK), Forestry,Environment
PEMURNIAN PARSIAL DAN KARAKTERISASI AMILASE DARI BAKTERI LAUT ARTHROBACTER ARILAITENSIS LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it?s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
THE EFFECT OF BIO-VA MYCORRHIZAE INOCULATION ON BIOMASS OF TREES AND CARBON STOCK OF EIGHT FOREST TREE SPECIES IN BODOGOL PLANTATION-SUKABUMI, INDONESIA Sukiman, Harmastini; Heriyanto, NM.
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2309

Abstract

Studi tentang pengaruh Bio-Mycorrhizae (BIO-VAM) pada biomasa dari delapan species tanaman dan stok karbonnyadilakukan dengan metoda destruksi sampel dari tanaman yang terpilih di Bodogol, Sukabumi, Jawa Barat. Data yangdidapat dari penelitian menunjukkan bahwa inokulasi Bio-VAM menunjukkan hasil yang positif terhadap biomasatanaman dan stok karbon. Volume biomasa tanaman tertinggi ditunjukkan oleh species tanaman umbrella tree (Maesopsisemenii. Eng) yakni 387,5 g per tanaman apabila diinokulasi dengan mikorisa dan 211,6 g per tanaman tanpa mikorisadiikuti dengan sengon buto (Enterolobium cyclocarpa Griseb), rasamala (Altingia excelsa Noronhae), manglid (Magnoliablumei Prantl), sawo duren (Chrysophyllum cainito L)., ki bereum (Adinandra dumosa Jack) bisbul (Diospyros blancoiDesr.) dan biomasa tanaman terendah ditunjukkan oleh species podocarp/ jamuju (Podocarpus imbricata R.Br) 82,76 gdengan inokulasi mikorisa dan 65,77 g tanpa inokulasi mikorisa. Karbon stok tertinggi ditunjukkan oleh tiga spesiestanaman yakni umbrella tree (Maesopsis emenii. Eng.) 3,23 ton C/ha dengan inokulasi mikorisa sementara tanaman kontrolmenunjukkan 1,76 ton C /ha, dan 11,85 ton CO2 /ha dan 6,46 CO2 /ha. Diikuti oleh sengon buto (Enterolobium cyclocarpaGriseb), rasamala (Altingia excelsa Noronha) Kontribusi karbon stok terendah ditunjukkan oleh podocarp (Podocarpusimbricata Blume) yakni 0,07 ton C/ha (dengan inokulasi BioVAM ) dan 0.05 ton C/ha (tanpa inokulasi BioVam) 0,26ton CO2 /ha dan 0,18 ton CO2. Tujuan dari penelitian ini adalah menganalisa pengaruh inokulasi Bio-VA Mycorrhizaeterhadap produksi biomasa tanaman dan stok karbon selama pertumbuhan tanaman.Kata Kunci: BIO-VAMycorrhizae, inokulasi, biomas tanaman hutan , stok karbon.
KAJIAN GEN AMELY GAJAH SUMATRA (ELEPHAS MAXIMUS SUMATRANUS) Zein, Moch Syamsul Arifin; Sulandari, Sri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2319

Abstract

Sumatran elephants (Elephas maximus sumatranus) is endemic of Asian elephants in Indonesia, an endangered animal andlisted on Appendix I of CITES. Therefore, information on the status and distribution of the population of Sumatranelephants, including the distribution of genetic diversity is necessary to facilitate the development of adequate conservationand management strategies. The purpose of this research was to use Amely gene to trace paternal lineage of Sumatranelephants based on Y chromosome variation. A total of 22 blood samples of male Sumatran elephants were collected inSumatra (Way Kambas , Seblat, Bentayan, Sugihan, dan Bukit Serelo Lahat). We amplified intronic regions of the Ylinkedgene (Amely) using published primer sequences (Amely-R2 and Amely-F2) and sequenced. Sequences generatedfrom this study, aligned with reference sequences available in the GenBank, namely Elephas maximus (AY823325.1),Loxodonta Africana (AY 823320.1; AY 823321.1), Loxodonta cyclotis (AY823322.1; AY8233223.1, AY 823324.1).Neighbour Joining tree of Sumatran elephants was performed using MEGA version 5.2.2. The analysis results of 22-maleSumatran elephants, indicating that no diversity (no variation) of the Y chromosome obtained among the population ofSumatran elephants. Sumatran and Asian elephants have the same haplotypes. Further results confirmed that the savannaelephants (Loxodonta africana ) and the forest elephants (Loxodonta cyclotis ) formed two (2) separate clades, which showstwo different species. Results obtained in this study may help to design future conservation programs for the species.Keywords: Sumatran elephant, Amely gene, Y chromosome, Genbank, haplotype
PRODUKTIVITAS DAN SERAPAN NUTRIEN HARIAN SPIROGYRA SP. DAN HYDRODICTYON SP. Pratiwi, Niken Tunjung Murti; Ayu, Inna Puspa; Nugraha, Bayu
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2325

Abstract

This research aimed to analyze the potency of primary productivity (PP) and daily nutrients uptake ofSpirogyra sp. and Hydrodictyon sp. The research was conducted using Randomized Block Design andCompletely Randomized Design. Randomized Block Design was used to determine the difference of PP ofboth filamentous microalgae in different time periods. Completely Randomized Design was used to determinethe difference of daily net primary productivity and total amount of nutrients utilization by both filamentousmicroalgae in the same light intensity. The observations showed that Spirogyra sp. dan Hydrodictyon sp. hassame PP (p>0,05), both in total (daily) and time periods. The morning PP of two filamentous microalgae weredifferent from the daylight (p<0,05), and the daylight has different value from the afternoon (p<0,05), but therewere similar value of PP between morning and afternoon (p>0,05). Spirogyra sp. and Hydrodictyon sp. hassimilar capability in uptaking nutrients daily and in time periods observations (p>0,05).Keywords: Hydrodictyon sp., nutrients, primary productivity, Spirogyra sp.
UJI TOLERANSI TANAMAN KENTANG HITAM (PLECTRANTHUS ROTUNDIFOLIUS (POIR.) SPRENG.) HASIL RADIASI SINAR GAMMA TERHADAP CEKAMAN KEKERINGAN Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2310

Abstract

Plectranthus rotundifolius (Poir.) Spreng. is one of the potential root crops to become an alternative food. The aim of thisstudy was to determine drought resistant status of Plectranthus rotundifolius irradiated by gamma irradiation. Thisexperiment was conducted in the Green House using a Completely Randomized Design with 2 factors and 5 replications.The first factor was plant number consisted of 7 accessions (D116, D69, M343, D40, M95, D3, and Klefa Imut/KI). Thesecond factor was the level of field capacity (FC) consisted of 100% FC, 60% FC, and 20% FC. The observed parameterwere vegetative and generative growth stage, stomatal conductance, leaf water potential, and chlorophyll content. The resultshowed that the resistance level of the 7 accessions can be devided into 3 categories: 1) The plants that were susceptible todrought i.e. D116, D69, M343, and KI; 2) The plants that were semi-tolerant to drought i.e. D40 and M95; and 3) The plantthat was tolerant to drought i.e. D3.Keywords: Plectranthus rotundifolius, gamma irradiation, drought stress
STRUKTUR DAN KOMPOSISI JENIS-JENIS POHON DI TAMAN NASIONAL GUNUNG RINJANI BAGIAN SELATAN, LOMBOK, NUSA TENGGARA BARAT Mansur, Muhammad
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2320

Abstract

Plant ecology study has been conducted in the southern part of Rinjani Mountain National Park on April 2015 to determinethe structure and composition of tree species. Four plots, 30 x 30 m each were established at four locations, those are; Pal-Belanda (1046 m asl), Pengorot-1 (929 m asl), Pengorot-2 (820 m asl), and Sebau (1134 m asl). The results showed thatthere are two types of forest in the sites, namely the secondary forest (Pal-Belanda, Pengorot-1 and Pengorot-2) and primaryforest (Sebau). From all plots (0.36 ha) we found 36 species of trees (trunk diameter > 5 cm) of 449 individuals, 28 genusand 21 families with a total Basal Area of 16.73 m2 (46.47 m2/ha). Shannon Diversity Index (H') in the Pengorot-2 recorded1,15, followed Pengorot-1 (1.13), Pal-Belanda (1.03) and Sebau (0.72). Saurauia pendula recorded as the dominant speciesin all site with Important Value Species (IVS) of 245% followed Dendrocnide microstigma (IVS = 162%), Platea excelsa(IVS=148.2%), Syzygium glomeruliferum (IVS=72%) and Symplocos cochinchinensis (IVS=49.3%). Myrtaceae andEuphorbiaceae were the common families in the sites. Structure and composition of tree species in the Pal-Belanda,Pengorot-1 and Pengorot-2 were relative similar (Similarity Index 70.3% and 69.4%) but different than in Sebau area(Similarity Index 24.9%).Keywords: Structure, composition, tree diversity, Rinjani, Lombok
BIOACUSTICS OF HYLARANA CELEBENSIS (PETERS, 1872) (ANURA: RANIDAE)FROM SULAWESI Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2306

Abstract

Hylarana celebensis (Peters, 1872) is an endemic frog to Sulawesi, the species being a member of family Ranidae.  The presence of the frog in its habitat is easily detected from its advertisement call; males usually call in a chorus, they call to each other in a large group. Bioacoustics of calls that a typical individual male of H. celebensis has not been described in detail, although it is very easy to find this species in freshwater swamps, permanent ponds, or slow-flowing waters in the lowland areas. The purpose of the bioacoustic analysis on H. celebensis?s calls that were recorded at Bahodopi area is to build a reference collection to be compared with H. celebensis?s bioacoustics to the other regions in Sulawesi. Because of the wide distribution of this frog in Sulawesi; population genetic structure of H. celebensis may also follow the population genetic structure of Ingerophrynus celebensis. Calls of H. celebensis have two types of calls, i.e. pure tone and pulse; however, pure tones have three variation, namely pure tone type 1, pure tone type 2 and pure tone type 3; however pulsed call has only one type. Keywords: Anura, Hylarana celebensis, bioacoustics, Sulawesi. 

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