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Keragaman Genetika Ramin [Gonystylus bancanus (Miq.) Kurz] dari Provinsi Riau Berdasarkan Profil Random Amplified Polymorphic DNA Kusumadewi, Yulita; Poerba, Yuyu S.; Partomiharjo, Tukirin
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3157

ABSTRACTGenetic Diversity of Ramin [Gonystylus bancanus (Miq.) Kurz] from Riau Province Based onRandom Amplified Polymorphic DNA Fingerprint. Gonystylus bancanus is a commercialtimber found only on peat swamp forests, scatteredly distributed in Sumatra and Kalimantan.Their existence is now under severe threat due to habitat conversion. One of the remainingnatural populations of ramin was in Riau Province, Sumatra. This study aimed to assessgenetic diversity of this species within their natural populations in Riau Province using RandomAmplified Polymorphic DNA (RAPD). RAPD profiles were obtained by performing PCRamplification using five arbitrary primers. One hundred and eleven putative loci of RAPD werescored and analysed using Popgene and NTSYS software. Eleven of RAPD bands werecommonly found in all populations and 16 bands were distinctively found in certain populations.These unique bands may serve as population diagnostic marker for such populations. Theaverage genetic diversity within population (0.1606) was lower than that of among populations(0.1894). Genetic differentiation (Gst) indicated that 95.56% of total genetic diversity in raminwas attributed to the differences among populations. The highest genetic diversity was foundin population 3 (He:0.1858) and 3 (I:0.2864), while the lowest genetic variation was observed inpopulation 1 (He: 0.1438) and 2 (I: 0.2201). Total genetic diversity for all population (Ht) was0.1982 with an average value of genetic diversity within populations (Hs) was 0.1606. The lowlevel of genetic diversity found in ramin with high population differentiation may suggest thatthese remaining populations was undergoing genetic bottleneck resulted from severe habitatfragmentation.Keywords: genetic diversity, populations, ramin, Gonystylus bancanus, RAPD.

Persilangan Pisang Liar Diploid Musa acuminata Colla var malaccensis (RIDL.) Nasution Sebagai Sumber Polen dengan Pisang Madu Tetraploid Poerba, Yuyu S.; Ahmad, Fajarudin; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Hybridization of wild diploid Musa acuminata Colla var malaccensis (Ridl.) Nasution as pollen source with teraploid Pisang Madu. Indonesia and South East Asia is the center of origin and genetic variability of bananas, specifically species Musa acuminata Colla. At least 15 varieties of wild Musa acuminata are found in Indonesia. Some of them have resistances for several diseases of bananas. One approach in banana breeding program is hybridizingbetween tetraploid parent and diploid parent of banana. This research was aimed to produce new triploid banana hybrid by crosses between tetraploid female parent âPisang Maduâ and wild diploid male parent Musa acuminata Colla var malaccensis (Ridl.) Nasution. From 302 crosses, 53.9% of crosses produced seeds. However, only 48.46% of seeds contained embryos, and only 1.27% of embryos grew and developed. Bunch weight and leaf length of hybridplants were not significantly different with female parent, tetraploid Pisang Madu. Plant height of the hybrids was not significantly different with male parent, wild diploid Musa acuminatavar malaccensis. Pseudostem diameter and leaf width of the hybrid plants were between female tetraploid parent and male wild diploid parent. RAPD profiles (DNA bands) of the hybrids were originated from the female parent, male parent and both parents were 38.46, 34.6, and 26.92%, respectively. The hybrids were confirmed to be triploids (3x=33).Keywords: banana, breeding, hybrids, tetraploid, wild diploid, Pisang Madu, Musa acuminata Colla var malaccensis (Ridl.) Nasution

Pembentukan dan Penampilan Pisang Rejang Hibrid Triploid Hasil Persilangan Pisang Rejang Mixoploid Dengan Pisang Rejang Diploid Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids

Karakterisasi Pisang Hibrid Tetraploid âGRNKâ Hasil Persilangan Pisang Triploid âGorohoâ dengan Pisang Iiar Diploid Musa acuminata Colla var. nakaii Nasution Poerba, Yuyu S.; Martanti, Diyah; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThe use of tetraploid banana in triploid banana breeding has been conducted through 4x x 2x crossing and/or its reciprocals, 2x x 4x crossing. However, the availability of tetraploid banana is naturally very limited, therefore artificially induced tetraploid banana is needed. The present research is aimed to generate tetraploid banana plants by crossing triploid banana âGorohoâ as female parent with wild diploid banana, Musa acuminata var. nakaii Nasution as male parent, and to characterize the tetraploid hybrid. 94 crossings were made by pollinating stigma of Pisang Goroho with polen of M. acuminata var. nakaii. Embrios resulted from hybridization were in vitro rescued and propagated. Hybrid plantlets were then acclimatized in the green house and seedlings were planted in the field for two cycles of banana reproduction. Ploidy identification, morphology and molecular characteristics were also conducted for two cycles of banana reproduction. Results of the experiment showed that 9 hybrid seeds were produced from 94 pollinations. However, only 2 hybrid embryos grew and developed into shoots and from two shoots only one shoot was survived. The one shoot were then in-vitro propagated to produce 21 hybrids planted in the field. After being evaluated for two plant reproductive cycles, all hybrid plants were tetraploids (2n=4x=44). The hybrid plants had drooping leaves, bigger fruit diameter compared to both parents, and blunt fruit tip. DNA (RAPD) profiles of tetraploid hybrid showed that 37.5% of the bands were from female parent, 37.5% from male parents, and 25% from both parents. The tetraploid hybrid âGRNKâ is expected to be useful in triploid banana breeding in the future.Key Words: Hybrid banana, tetraploid, Goroho, Musa acuminata Colla var. nakaii Nasution

ANALISIS KERAGAMAN GENETIK KENTANG HITAM Yulita, Kusumadewi Sri; Ahmad, Fajarudin; Martanti, Diyah; Poerba, Yuyu S.; Herlina, Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.

PENAMPILAN RANDOM AMPLIFIED POLYMORPHIC DNA PADA Azadirachta indica A. Juss DARI TAMAN NASIONAL BALURAN Ahmad, Fajarudin; Poerba, Yuyu S.
Jurnal Teknologi Lingkungan Vol. 11 No. 1 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Azadirachta indica A. Juss (Apocynaceae) is a large tree of the lowland tropical rainÃ‚Â forest of Southeast Asia that occurs in Thailand, the Malay Peninsula, on the island ofÃ‚Â Java (East Java) and Lesser Sunda Islands. Its economic value was in its wood (timber),Ã‚Â and as medicinal plant. The information on genetic diversity of the species is very limited.Ã‚Â Hence studies were initiated and genetic diversity estimated using RAPD markers inÃ‚Â 27 accessions of A.indica procured from three geographical regions of TN Baluran andÃ‚Â Balai Litbang Kehutanan. Seven selected Operon primers (10 mer) generated a totalÃ‚Â of 133 consistent amplification products ranging from 132 bp to 5.6 Kb. The clusterÃ‚Â analysis separated the 27 individuals into 2 clusters. The range of genetic dissimilarityvalue among samples was from 0.07 to 0.33, while genetic distance among populationsÃ‚Â was from 0.04 to 0.10. These values showed that A. indica from TN Baluran was notÃ‚Â genetically diverse population.Ã‚Â Key words: Azadirachta indica, genetic diversity, RAPD

PERSILANGAN PISANG LIAR DIPLOID MUSA ACUMINATA COLLA VAR MALACCENSIS (RIDL.) NASUTION SEBAGAI SUMBER POLEN DENGAN PISANG MADU TETRAPLOID Poerba, Yuyu S.; Ahmad, Fajarudin; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3076

Hybridization of wild diploid Musa acuminata Colla var malaccensis (Ridl.) Nasution as pollen source with teraploid Pisang Madu. Indonesia and South East Asia is the center of origin and genetic variability of bananas, specifically species Musa acuminata Colla. At least 15 varieties of wild Musa acuminata are found in Indonesia. Some of them have resistances for several diseases of bananas. One approach in banana breeding program is hybridizingbetween tetraploid parent and diploid parent of banana. This research was aimed to produce new triploid banana hybrid by crosses between tetraploid female parent ?Pisang Madu? and wild diploid male parent Musa acuminata Colla var malaccensis (Ridl.) Nasution. From 302 crosses, 53.9% of crosses produced seeds. However, only 48.46% of seeds contained embryos, and only 1.27% of embryos grew and developed. Bunch weight and leaf length of hybridplants were not significantly different with female parent, tetraploid Pisang Madu. Plant height of the hybrids was not significantly different with male parent, wild diploid Musa acuminatavar malaccensis. Pseudostem diameter and leaf width of the hybrid plants were between female tetraploid parent and male wild diploid parent. RAPD profiles (DNA bands) of the hybrids were originated from the female parent, male parent and both parents were 38.46, 34.6, and 26.92%, respectively. The hybrids were confirmed to be triploids (3x=33).Keywords: banana, breeding, hybrids, tetraploid, wild diploid, Pisang Madu, Musa acuminata Colla var malaccensis (Ridl.) Nasution

PEMBENTUKAN DAN PENAMPILAN PISANG REJANG HIBRID TRIPLOID HASIL PERSILANGAN PISANG REJANG MIXOPLOID DENGAN PISANG REJANG DIPLOID Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids

KERAGAMAN GENETIKA RAMIN [GONYSTYLUS BANCANUS (MIQ.) KURZ] DARI PROVINSI RIAU BERDASARKAN PROFIL RANDOM AMPLIFIED POLYMORPHIC DNA Kusumadewi, Yulita; Poerba, Yuyu S.; Partomiharjo, Tukirin
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3157

ABSTRACTGenetic Diversity of Ramin [Gonystylus bancanus (Miq.) Kurz] from Riau Province Based onRandom Amplified Polymorphic DNA Fingerprint. Gonystylus bancanus is a commercialtimber found only on peat swamp forests, scatteredly distributed in Sumatra and Kalimantan.Their existence is now under severe threat due to habitat conversion. One of the remainingnatural populations of ramin was in Riau Province, Sumatra. This study aimed to assessgenetic diversity of this species within their natural populations in Riau Province using RandomAmplified Polymorphic DNA (RAPD). RAPD profiles were obtained by performing PCRamplification using five arbitrary primers. One hundred and eleven putative loci of RAPD werescored and analysed using Popgene and NTSYS software. Eleven of RAPD bands werecommonly found in all populations and 16 bands were distinctively found in certain populations.These unique bands may serve as population diagnostic marker for such populations. Theaverage genetic diversity within population (0.1606) was lower than that of among populations(0.1894). Genetic differentiation (Gst) indicated that 95.56% of total genetic diversity in raminwas attributed to the differences among populations. The highest genetic diversity was foundin population 3 (He:0.1858) and 3 (I:0.2864), while the lowest genetic variation was observed inpopulation 1 (He: 0.1438) and 2 (I: 0.2201). Total genetic diversity for all population (Ht) was0.1982 with an average value of genetic diversity within populations (Hs) was 0.1606. The lowlevel of genetic diversity found in ramin with high population differentiation may suggest thatthese remaining populations was undergoing genetic bottleneck resulted from severe habitatfragmentation.Keywords: genetic diversity, populations, ramin, Gonystylus bancanus, RAPD.

KARAKTERISASI PISANG HIBRID TETRAPLOID â€˜GRNKâ€™ HASIL PERSILANGAN PISANG TRIPLOID â€˜GOROHOâ€™ DENGAN PISANG IIAR DIPLOID MUSA ACUMINATA COLLA VAR. NAKAII NASUTION Poerba, Yuyu S.; Martanti, Diyah; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3393

ABSTRACTThe use of tetraploid banana in triploid banana breeding has been conducted through 4x x 2x crossing and/or its reciprocals, 2x x 4x crossing. However, the availability of tetraploid banana is naturally very limited, therefore artificially induced tetraploid banana is needed. The present research is aimed to generate tetraploid banana plants by crossing triploid banana ?Goroho? as female parent with wild diploid banana, Musa acuminata var. nakaii Nasution as male parent, and to characterize the tetraploid hybrid. 94 crossings were made by pollinating stigma of Pisang Goroho with polen of M. acuminata var. nakaii. Embrios resulted from hybridization were in vitro rescued and propagated. Hybrid plantlets were then acclimatized in the green house and seedlings were planted in the field for two cycles of banana reproduction. Ploidy identification, morphology and molecular characteristics were also conducted for two cycles of banana reproduction. Results of the experiment showed that 9 hybrid seeds were produced from 94 pollinations. However, only 2 hybrid embryos grew and developed into shoots and from two shoots only one shoot was survived. The one shoot were then in-vitro propagated to produce 21 hybrids planted in the field. After being evaluated for two plant reproductive cycles, all hybrid plants were tetraploids (2n=4x=44). The hybrid plants had drooping leaves, bigger fruit diameter compared to both parents, and blunt fruit tip. DNA (RAPD) profiles of tetraploid hybrid showed that 37.5% of the bands were from female parent, 37.5% from male parents, and 25% from both parents. The tetraploid hybrid ?GRNK? is expected to be useful in triploid banana breeding in the future.Key Words: Hybrid banana, tetraploid, Goroho, Musa acuminata Colla var. nakaii Nasution

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