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E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)
Published by Universitas Udayana
ISSN : 23016515     EISSN : -     DOI : -
Core Subject : Agriculture,
E-Jurnal Agroekoteknologi Tropika (JAT) adalah peer-reviewed, open access jurnal, diterbitkan oleh Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana. JAT menerbitkan hasil peneitian original dan review artikel dalam bidang Ilmu tanah dan lingkungan, Agronomi, hama dan penyakit tumbuhan, dan arsitektur lanskap. Penulis diharapkan mengimkan karya tulisnya yang belum pernah dipublikasikan, sedang di-review atau sedang dipertimbangkan untuk diterbitkan di jurnal atau media lain secara lengkap. Ruang lingkup JAT mencangkup, tetapi tidak terbatas pada bidang berikut: kimia dan kesuburan tanah, biologi tanah, fisika dan konservasi tanah, manajemen suberdaya lahan, pengendalian hama dan penyakit terpadu, hama tanaman, penyakit tanaman, biopestisida, bioteknologi tumbuhan, agronomi dan hortikultura, benih dan pemuliaan tanaman, ekofisiologi, dan arsitektur lanskap.
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Articles 8 Documents
Search results for , issue "Vol.3, No.3, Juli 2014" : 8 Documents clear
Uji Efektivitas Beberapa Teknik Ekstraksi dan Dry Heat Treatment terhadap Viabilitas Benih Tomat (Lycopersicum esculentum Mill.) I WAYAN GUNARTA; I GUSTI NGURAH RAKA; A.A.M. ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Efectivity Test for Some Extraction Techniques and Dry Heat Treatment on Tomato Seed (Lycopersicum esculentum Mill.) This experiment aimed to obtain the most effective extraction method and determine the effect of dry heat treatment to produce high quality tomato seeds. The design used was completely randomized design with 3 treatments and 9 replications. The treatments include: (1) Bl : fresh tomato seeds taken, immediately dried to 5% moisture content, (2) Ba: fresh tomato seeds taken, then soaked in water for 24 hours, dried to 5% moisture content, (3 ) Bh: fresh tomato seeds taken, then soaked in a solution of 2% HCl for 2 hours, then dried until the moisture content of 5%. Seeds obtained from each extraction technique is divided into two lots, one lot for Dry Heat Treatment (DHT) and the other one lot for Non Dry Heat Treatment (NDHT). The variables measured were the number of seed moisture content of 5% by weight of unity, purity of the seeds, the total viability, potential viability, vigor speed germination, and seedling growth vigor. The results showed the significant diference between extraction treatment to all of variables tested, while the granting of DHT on the seed does not provide a response to tomato seed quality, on all variables tested. Bh extraction treatment showed the best results with the highest values  on all of variables tested.   Key word : tomato seeds, extraction, dry heat treatment, viability and vigor.
Deteksi Molekuler Cucumber Mosaic Virus (Cmv) pada Tanaman Gamal (Gliricidia Sepium) Sebagai Barier pada Pertanaman Cabai IDA BAGUS GDE PRANATAYANA; I GEDE RAI MAYA TEMAJA; KETUT YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Molecular Detection of Cucumber Mosaic Virus (CMV) In Gliricidia Plant (Gliricidia sepium) as Barrier at Chili pepper Crop The aims of the study are in order to symptoms characteristic identity the CMV infection on gliricidia plant and symptoms characteristic the CMV infection on chili pepper plant, and order to presence identity the CMV on gliricidia plant and presence the CMV on chili papper plant at Kerta village base on molecular analysis. The serology method was used to detect CMV virus is DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was used for molecular analysis. The study include 1) the observation of mosaic in field, 2) preservation of plant samples (chili pepper and gliricidia) infected with mosaic virus, 3) serology test with DAS-ELISA, 4) molecular detection with RT-PCR. The research proved that symptoms characteristic the CMV infection on gliricidia plant identical with symptoms characteristic the CMV infection on chili pepper plant and presence the CMV on gliricidia plant identical with presence the CMV on chili pepper plant. symptoms of mosaic characteristic in gliricidia and chili pepper found on the field among others, mosaic (striped), contract and roll with variations of symptoms that light green mosaic with chlorotic and dark green mosaic symptoms in gliricidia and chili pepper plants. DAS-ELISA test results showed gamal and chili pepper samples infected positive of Cucumber Mosaic Virus (CMV). RT-PCR technique successfully amplify the target DNA fragment size of 657 bp. Based on these studies, the farmers need to be aware of the existence of the plant used as a plant barrier on chilli crop, because gliricidia potential as alternative hosts of CMV.   Key word : Chili pepper, cucumber  mosaic virus, gliricidia, molecular
Uji Efektivitas Fungisida Alami dan Sintetis dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat yang Disebabkan oleh Fusarium oxysporum f.sp. lycopersici LASTRI APRIANI; DEWA NGURAH SUPRAPTA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT The Test of Natural and Synthetic Fungicides Effectiveness in Controlling Fusarium Wilt of Tomato Plants Caused by Fusarium oxysporum f.sp. lycopersici Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici is one of important diseases in tomato plants. The emersion of the disease can result in huge loss for farmers. The use of synthetic fungicides that has been widely utilized all this time by farmers to control diseases caused by Fusarium oxysporum f.sp. lycopersici has adverse environmental impacts, therefore it is required biological fungicide that is more environmentally friendly. This study is purposed to testing the effectiveness of some natural fungicides and synthetic fungicide, is like a betel leaf, Trichoderma harzianum fungus, Klebsiella pneumoniae, and benomyl for suppress F. oxysporum f.sp. lycopersici and restrained Fusarium wilt diseases . The result of this study shows that the biological fungicide of betel leaf extract can inhibit the pathogenic F. oxysporum f.sp. lycopersici by 76.11 % and the percentage of inhibition potency of other benomyl fungicides reaches 61.11 % , while Klebsiella pneumoniae is by 54.42 % , and 20.98 % for Trichoderma harzianum on PDA medium. The result of field test shows that the extract of betel leaf can suppress Fusarium wilt by 5 % , T. harzianum , benomyl and K. pneumoniae are able to suppress Fusarium wilt respectively by 2.5 %. The use of T. harzianum and K. pneumoniae as biological control has the same effectiveness with synthetic fungicide in order to suppress Fusarium wilt.   Keywords: fusarium wilt, fusarium oxysporum f.sp. lycopersici, biological fungicide
Daya Simpan Benih Tomat (Lycopersicum esculentum mill.) Hasil Beberapa Teknik Ekstraksi I NYOMAN RAGANATHA; I GUSTI NGURAH RAKA; I GEDE KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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AbstractTomato Seed (Lycopersicum esculentum Mill.) Storability of Some Extraction Techniques Results                 Shelf life tomato seeds produced by different seed extraction techniques studied using completely randomized design ( CRD ) with three treatments of seed extraction technique and nine replicates. Three seed extraction techniques used are: extraction technique with 2 % HCl, washed with water extraction techniques, and extraction techniques with immersion in water for 24 hours. The seeds are dried until the moisture content ranges from 5 %, then stored at room temperature. Observations before storage include: seed moisture content, the amount of seed moisture content of 5 % per 5 g of seeds, seed purity, germination and speed of germination. Observations during storage include: seed moisture content,  germination and speed of germination. The results showed that the extraction technique of soaking seeds with 2 % HCl able to produce seeds with the lowest percentage of impurities so that the absorption of moisture during the storage period was also the lowest. Extraction technique of soaking seeds with 2 % HCl able to maintain the viability of the seed germination above 80 % until the 12 -week storage period at speeds high enough to germinate. While the seeds of other extraction techniques which have a shorter shelf life. Further research on the storage technique that is able to maintain the viability and vigor of tomato seeds for a longer shelf life.   Key words : Tomato seeds, extraction, seed storage, viability, vigor
Pengaruh Penggunaan Mulsa Plastik terhadap Hasil Tanaman Cabai Rawit (capsicum frutescens l.) di Luar musim di Desa Kerta I GEDE PUTU DARMAWAN; I DEWA NYOMAN NYANA; I GUSTI ALIT GUNADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Influence of Plastic Mulch on Crop Chili Pepper (Capsicum frutescens L.) Off  Season in the Kerta village. Chili is one of the important horticultural commodities in Indonesia and has a high economic value. To maintain its supplies, cultivation technology that able to produce the commodities continuously is needed by applying technology in the off-season cultivation. This study aims to determine the effect of plastic mulch on chili crop in the off-season. The experimental design was used a randomized block design with 3 treatments and 9 replications. The treatment given is the use of mulch consisting of silver plastic mulch (MP), black plastic mulch (MH), and without mulch/control (K). The variables observed in this study, ware, plant height, number of branches, soil temperature, symptoms of viral diseases, and yield per hectare. The results showed the plastic mulch give good results on chili crop in the off-season. However, the highest results of the chili plants was in the silver plastic mulch treatments with plant height (95.64 cm), number of branches (13.11 piece), and yield (12.31 ton/ha).   Keywords: chili pepper, off season, plastic mulch, yield
Pengaruh Infeksi Beberapa Jenis Virus terhadap Penurunan Hasil pada Tanaman Cabai Rawit (Capsicum frutescens L.) I WAYAN SUKADA; I MADE SUDANA; I DEWA NYOMAN NYANA; GEDE SUASTIKA; KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Effect of Infection of Some Viruses to the Decresing Yield on Rawit Pepper (Capsicum frutescens L.) This study aims to identify the types of viruses that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, and to know the influence of some kind of viruses infection on yield decline. To verify the type of virus is done by taking a sample of the pepper plant leaf showing symptoms of the virus that has been demonstrated through serological and molecular tests. The results of this study indicate that the mosaic symptomatic chilli pepper plants were infected by some kind of virus such as CMV, TMV, and ChiVMV. Chilli pepper shows asymptomatic yellow were infected by the PepYLCV and chlorosis were infected by Polerovirus. The result of fruit chili harvest were showed the higher yields on the healthy plants with average 16.01 tonnes/ha, while the chilli that showed chlorosis symptomps were 8.42 tonnes/ha, and yellow symptomps were 3.07 tonnes/ha. The lowest were in the plants that shows mosaic symptomp with average 2.52 tonnes/ha. The virus infection caused the losses of yield of 47.40% in chlorosis symptomatic plants, 80.82% in the yellow symptomatic plant and 84.25%, in the mosaic symptomatic plants compared to healthy plants. High yield loss in the mosaic symptomatic plants were caused by the highest infection on chiilli pepper that infected by some kind of viruses.   Keywords : Chili pepper , mosaic, chlorosis, and yellow
Isolasi dan Identifikasi Agrobacterium tumefaciens dari Tanaman Wortel (Daucus carota L.) YOLANDA HASSIAN MANALU; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch’s postulates. The result of this study determined that carrot’s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch’s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
Isolasi dan Identifikasi Agrobacterium Tumefaciens pada Tanaman Mawar (Rosa sp.). NADIAH SILITONGA; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens At Plant roses (Rosa sp.). Agrobacterium tumefaciens caused crown gall tumor in many of dicotyledonous plants. The purpose of this study tried to isolate and identify the A. tumefaciens from rose plant. Various techniques were used such as selection media, colonies shape and color, Koch's postulate test, DNA isolation and agarose gel elektroforesis. The results of this study showed that A. tumefaciens can be isolated through culturing in LB medium and selection AB medium that was specific for A. tumefaciens. The isolate caused crown gall tumor on carrot slice 3 weeks after inoculation by using a modified Koch’s postulate test. The characteristics of colony formed in this research are round shaped, cream coloured with pink tint, smooth edge, and convex elevation. DNA isolation and its running in agarose gel electroforesis showed positive result.   Key words : Agrobacterium tumefaciens, AB medium and Agarose gel electroforesis.

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