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All Journal JURNAL ANALIS LABORATORIUM MEDIK Bali Medika Jurnal Jurnal Media Sains Jurnal Ilmiah Medicamento Jurnal Kesehatan Terpadu Pena Dimas: Jurnal Pengabdian Masyarakat Multidisciplinary Indonesian Center Journal Bali International Scientific Forum
Sutrisna Putra, I Gusti Putu Agus Ferry
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Efektifitas ekstrak kulit batang kemuning (murraya paniculate (l) jack) sebagai penurun low density lipoprotein darah tikus wistar yang mengalami hiperkolesterolemia: The effectiveness of the bark extract of kemuning as a lowering of low density lipoprotein (ldl) in wistar rats with hypercholesterolemia Prihatiningsih, Diah; Putra, I Gusti Putu Agus Ferry Sutrisna; Juliantara, I K. Putra
Bali Medika Jurnal Vol 9 No 2 (2022): Special Issue Bali Medika Jurnal Vol 9 No 2 Oktober 2022
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v9i2.309

Abstract

Kolesterol tinggi merupakan masalah kesehatan yang serius. Penyakit ini disebabkan penumpukan kolesterol dalam bentuk low density lipoproptein  di pembuluh darah sehingga menimbulkan terjadinya penyumbatan pembuluh darah jantung. Penggunaan simvastatin  da;a, hyperlipidemia dapat menimbulkan efek samping pada penggunaan jangka panjang. Oleh karena itu perlu pengembangan obat lain yang berasal dari bahan herbal (tanaman). Salah Satu tanaman yang memiliki potensi sebagai penurun hiperkolesterolemia adalah kemuning. Tujuan dari penelitian ini adalah mengukur ekstrak kulit batang kemuning sebagai penurun Low Density Lipoprotein (LDL) pada tikus galur wistar yang mengalami hiperkolesterolemia.  Penelitian ini merupakan eksperimental, dengan rancangan One Group Pretest and Posttest Design untuk mengetahui efektifitas ekstrak kulit batang kemuning sebagai penurun low density lipoprotein pada tikus wistar yang hiperkolesterolemia.  Penelitian ini menggunakan enam kelompok, yaitu dua kelompok kontrol dan empat kelompok eksperimental, dengan randomisasi sederhana. Pada penelitian terdapat pretest sebelum diberi perlakuan, dengan demikian hasil perlakuan dapat diketahui lebih akurat karena dapat membandingkan antara keadaan sebelum diberi perlakuan dan setelah perlakuan. Sampel. Ekstrak kulit batang kemuning mengandung Flavonoid dengan  kandungan 892,35 mg/100 g QE , Fenol 2089,345/100 g GAE dan Tannin 1876,87 mg/100g GAE. Ekstrak kulit batang kemuning dapat menurunkan low density lipoprotein (LDL) pada tikus galur wistar yang mengalami hiperkolesterolemia, dengan penurunan terbaik pada  P3 yaitu sebesar 50,05% untuk pemberian ekstrak sebanyak 69 g/ 150 BB. Ekstrak kulit batang kemuning juga dapat menaikkan high density lipoprotein (HDL) setelah pemberian ekstrak kulit batang kemuning pada tikus galur wistar yang mengalami hiperkolesterolemia, dengan peningkatan terbaik ditunjukkan P3 yaitu sebesar 29,48%      Cholesterol is a serious health problem. This disease is caused by cholesterol in the form of low density lipoprotein in the blood vessels, causing the occurrence of heart blood vessels. The use of simvastatin and hyperlipidemia can cause side effects in long-term use. Therefore, it is necessary to develop other drugs derived from herbal ingredients (plants). One of the plants that has the potential to reduce hypercholesterolemia is yellow. The purpose of this study was to measure the bark extract of kemuning as a lowering of Low Density Lipoprotein (LDL) in Wistar rats with hypercholesterolemia. This research was experimental, with a One Group Pretest and Posttest Design to determine the effectiveness of the extract of the bark of kemuning as a lowering low density lipoprotein in hypercholesterolemic wistar rats. This study used six groups, namely two control groups and four experimental groups, with simple randomization. In the study, there was a pretest before being given treatment, with the treatment it can be known more accurately because it can compare between the conditions before being given treatment and treatment. Sample. Yellow stem bark extract contains flavonoids with 892.35 mg/100 g QE, 2089.345/100 g GAE phenols and 1876.87 mg/100 g GAE tannins. Yellow stem bark extract can reduce low density lipoprotein (LDL) in Wistar rats with hypercholesterolemia, with the best decrease in P3 which is 50.05% to provide extract as much as 69 g/150 BW. Kemuning bark extract can also increase high density lipoprotein (HDL) after presenting the yellow skin extract in Wistar strain rats experiencing hypercholesterolemia, with the best increase shown in P3 stems, which is 29.48%
OPTIMALISASI PENGETAHUAN PETUGAS AHLI TEKNOLOGI LABORATORIUM MEDIS DALAM PENANGANAN SPESIMEN FESES DENGAN PENAMBAHAN ETANOL Idayani, Sri; Trisnadewi, Ni Wayan; Pramesti, Theresia Anita; Lestari, Ni Kadek Yuni; Putra, I Gusti Putu Agus Ferry Sutrisna
PENA DIMAS: Jurnal Pengabdian Masyarakat Vol 3, No 2 (2025): Pena Dimas: Jurnal Pengabdian Masyarakat
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/penadimas.v3i2.27188

Abstract

In improving the quality of laboratory examinations, Medical Laboratory Technologists (ATLM) need information about specimen storage, including specimen handling. One way to diagnose Soil Transmitted Helminths (STH) Infections is through fecal examination. With the limited number of fecal samples, this activity is expected to improve the knowledge and skills of ATLM at the Denpasar Utara III Health Center, so it is important to have socialization and demonstrations regarding the handling of fecal specimens. The purpose of this activity is to improve the ability of ATLM in handling fecal specimens with the addition of ethanol to the quality of worm eggs. The method of implementing this community service is carried out by providing socialization in the form of education through lectures using powerpoint and LCD media and continued with demonstrations and evaluations in the form of the results of filling out the google form before and after education and demonstrations. ATLM officers were very enthusiastic in participating in the activity and actively asked questions both about handling fecal specimens and making ethanol solutions. In addition, the indicator of the success of this activity is an increase in the ability to carry out laboratory examinations, especially in parasite examinations, with an emphasis on proper specimen handling. By adding a diluted ethanol solution as needed to maintain the quality of the specimen in preventing damage to the specimen before further examination, so that the quality of the worm eggs obtained through microscopic examination will be better.
Pengaruh Variasi Dosis Dekstrometorfan Terhadap Kadar Serum Glutamic Pyruvic Transaminase dan Gamma Glutamyl Transferase Pada Tikus Wistar Iswari, Ni Wayan Gita; Putra, I Gusti Putu Agus Ferry Sutrisna; Widayanti, Ni Putu
Bali International Scientific Forum Vol. 1 No. 1 (2020): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v1i1.127

Abstract

Background: Dextromethorphan is an antitussive drug commonly found in cough medicine, besides that dextromethorphan has the function as an expectorant or cough suppressant. Dextromethorphan is widely misused by the community, especially adolescents. Objective: to determine the effect of variations in dextromethorphan doses on SGPT and GGT levels in wistar rats. Method: using an experimental research design using the pretest-posttest control group design method. The analysis technique uses SPSS One Way Annova analysis. Results: Giving doses of dextromethorphan can increase levels of SGPT and GGT, namely in group P1 of 5.68% and 14.06%, P2 of 16.67% and 26.82%, and P3 of 20.95% and 36.54 %. Conclusion: There are effects of variations in dextromethorphan doses on increasing levels of SGPT and GGT in Wistar rats. Suggestion: It is necessary to conduct examination parameters and need to do research with more varied and longer doses.
Pengaruh Ekstrak Batang Dan Daun Pakis Sayur (Diplazium Esculentum) Terhadap Kadar Hemoglobin, Hematokrit dan Jumlah Eritrosit Pada Tikus Putih Jantan yang Disuntikkan Natrium Nitrit (NaNO2) Sashmita, Geby; Putra, I Gusti Putu Agus Ferry Sutrisna; Widayanti, Ni Putu
Bali International Scientific Forum Vol. 1 No. 1 (2020): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v1i1.128

Abstract

Background: Anemia is a decrease in hemoglobin level, hematocrit value and the number of erythrocytes in the body due to a lack of iron intake in the body. One plant that is rich in iron is vegetable ferns (diplazium esculentum). This research was conducted at the Animal Unit Laboratory of the Faculty of Medicine, Udayana University. Objective: To determine the effect of vegetable ferns and stem extracts on hemoglobin levels, hematocrit values and the number of erythrocytes. Method: This study uses true experimental design. Results: The results of this study prove that stem fern extract and vegetable ferns can increase hemoglobin levels, hematocrit values and the number of erythrocytes in experimental male rats with a dose of 0.18 grams, 0.36 grams and 0.72 grams. Conclusion: There was a significant effect on male white mice with anemia treatment after being given stem and vegetable fern leaf extract, where the administration of vegetable fern extracts and ferns with a dose of 0.72 grams resulted in an increase in hemoglobin levels of 52.07%, a hematocrit value of 32, 91% and the number of erythrocytes is 29.41%.
Pengaruh Pemberian Variasi Dosis Rifampisin Terhadap Kadar Gamma Glutamyl Transferase dan Alkaline Phosphatase Pada Tikus Putih Galur Wistar Jeharu, Sofiana Agustin; Putra, I Gusti Putu Agus Ferry Sutrisna; Widayanti, Ni Putu
Bali International Scientific Forum Vol. 1 No. 1 (2020): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v1i1.129

Abstract

Background: Tuberculosis is a contagious infectious disease caused by the bacterium Mycobacterium tuberculosis. Rifampicin is a first-line drug for the treatment of tuberculosis. Rifampicin has a bactericidal effect and the sterilizing effect is effective against intracellular and extracellular tuberculosis bacteria. In addition, one of the side effects of using rifampicin is hepatotoxicity. Objective: to determine the effect of rifampicin drug dosage on GGT and ALP levels in wistar rats. Methods: using an experimental study design with the pretest and posttest control group design method with the use of test animals 24 wistar rats divided into 4 groups. The technique of analyzing data using SPSS One Way Annova was followed by a Post-Hoc Test. Results: The results of the study showed an average increase in GGT levels in treatment groups 1, 2 and 3 was 29.5%, 40.9% and 55.4% and the mean increase in ALP levels in treatment groups 1, 2 and 3 was 27.5 %, 46.7% and 53.5%. The One Way ANOVA test results obtained p = 0.00 (p <0.05) followed by the Post Hoc test obtained α> 0.05. Conclusions: there was a significant effect on the levels of GGT and ALP after the administration of dose variations of the rifampicin drug in wistar strain white rats.
Perbandingan Hasil Creatinin Clirens Test (CCT) dan Estimasi Laju Filtrasi Glomerulus (eLFG) Pada Pasien Kemoterapi di Rumah Sakit Prima Medika Wijayanti, Sariati; Putra, I Gusti Putu Agus Ferry Sutrisna; Juliantara, I K. Putra
Bali International Scientific Forum Vol. 1 No. 1 (2020): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v1i1.130

Abstract

Background: Creatin clirens test (CCT) is a reliable examination to estimate LFG. To solve some weaknesses in the creatinine clirens test, we can use estimation of glomerulus filtration rate (eLFG). Estimation of glomerulus filtration rate (eLFG) can be used as the Cockcroft Gault (CG) formula, Modification of Diet in Renal Disease (MDRD), and Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI). Each formula has advantages and disadvantages. Objective: The aim of this study was to compare the results of CCT with eLFG (CG, MDRD, CKD-EPI) and the correlation between results of CCT and eLFG (CG, MDRD, CKD-EPI). Method: Research on the comparison of the results of creatinine clarity test (CCT) and estimation of glomerulus filtration rate (eLFG) is a type of comparative analytical study with a retrospective approach. Result: The results showed that there were no differences in the results of CCT and eLFG (CG, MDRD, CKD-EPI) (p > 0.05). In addition, there is correlation between CCT and eLFG (CG, MDRD, CKD-EPI) (r > 0.05). Conclusion: It can be concluded that there is no difference in the results of CCT and eLFG (CG, MDRD, CKD-EPI). In addition, there is a correlation between the results of CCT and eLFG (CG, MDRD, CKD-EPI).
CORRELATION BETWEEN HEMOGLOBIN A1c (HbA1c) LEVELS AND MICROALBUMINURIA IN DIABETES MELLITUS PATIENTS AT PRODIA SUNTER Putri, Maylina; Putra, I Gusti Putu Agus Ferry Sutrisna; Prihatiningsih, Diah
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 2 No. 4 (2025): Vol. 2 No. 4 Edisi Oktober 2025
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v2i4.1278

Abstract

Diabetes Mellitus (DM) is a disease caused by pancreatic failure in performing its function of producing insulin. A lack of insulin will lead to an increase in blood glucose levels, also known as hyperglycemia. Hemoglobin A1c and microalbuminuria are screening examinations used to detect the early onset of diabetic nephropathy. Diabetic nephropathy is defined as a clinical syndrome in diabetes mellitus patients characterized by persistent albuminuria, an irreversible decrease in glomerular filtration rate, and increased arterial blood pressure. The purpose of this study is to determine the relationship between Hemoglobin A1c and microalbuminuria in diabetes mellitus patients at Prodia Sunter. The research method used is quantitative analytic with a cross-sectional approach. The data collected were secondary data from the period of January–July 2024 with a total of 60 samples. Sampling was conducted using purposive sampling and met the inclusion criteria, namely patients over 60 years of age, Hemoglobin A1c value ≥7, diagnosed with diabetes mellitus, no history of thalassemia, and who had undergone Hemoglobin A1c and microalbuminuria examinations. The results of this study show a significant unidirectional positive relationship with a very strong correlation between Hemoglobin A1c levels and microalbuminuria in diabetes mellitus patients, with sig (2-tailed) = 0.000 and correlation coefficient value = 0.892. The relationship between Hemoglobin A1c levels and microalbuminuria is unidirectional positive, meaning that the higher the Hemoglobin A1c levels, the higher the microalbuminuria levels.
TINGKAT HEMOGLOBIN LANSIA: PERBEDAAN PENGUKURAN ANTARA DUA ALAT BERBEDA Prihatiningsih, Diah; Sutrisna Putra, I Gusti Putu Agus Ferry
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 1 No. 2 (2024): Vol. 1 No. 2 Edisi April 2024
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v1i2.105

Abstract

The examination of hemoglobin levels in the elderly is crucial as iron deficiency or anemia is often found in this age group. Various methods of hemoglobin examination are used in healthcare facilities. This study aims to identify the differences in hemoglobin levels using the Dirui BCC-3600 and Quick Check devices in the elderly population at Puskesmas Biromaru. This research is descriptive-comparative in nature. The sample consisted of 30 patients undergoing hemoglobin examination at the Puskesmas Biromaru laboratory between March 29th and April 30th, 2023. The sampling method used was accidental sampling, with venous blood samples from 30 respondents. The results of the study showed that the average hemoglobin level using the Dirui BCC-3600 device was 12.86 g/dL, while with the Quick Check device it was 11.77 g/dL. Normality testing was conducted using the Shapiro-Wilk test, which yielded a p-value of 0.023. A p-value < 0.05 indicates non-normal data distribution, thus followed by the Mann-Whitney test. The Mann-Whitney test resulted in a significant value (p) = 0.011. In conclusion, there is a significant difference in hemoglobin levels using the Dirui BCC-3600 and Quick Check devices in the elderly.
Pengaruh Waktu Penundaan Pemeriksaan dan Suhu Penyimpanan Specimen Darah Edta terhadap Jumlah Trombosit dan Platelet Distribution Width (PDW) Putra, I Gusti Putu Agus Ferry Sutrisna; Prihatiningsih, Diah
Jurnal Kesehatan Terpadu Vol. 9 No. 2 (2025): Jurnal Kesehatan Terpadu
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pemeriksaan jumlah trombosit merupakan uji penyaring penting dalam membantu penegakan diagnosis. Namun, penundaan pemeriksaan darah utuh sering terjadi di berbagai fasilitas pelayanan kesehatan, sehingga dapat memengaruhi stabilitas sel darah. Penelitian ini bertujuan untuk mengetahui pengaruh waktu penundaan dan suhu penyimpanan terhadap jumlah trombosit dan Platelet Distribution Width (PDW). Penelitian ini merupakan eksperimen laboratorium dengan rancangan posttest only control group design. Sampel darah dari 20 responden dibagi menjadi enam kelompok perlakuan berdasarkan kombinasi waktu penundaan (segera, 1 jam, 3 jam, dan 6 jam) dan suhu penyimpanan (18–27 °C dan 2–8 °C). Data dianalisis menggunakan uji general linear model.Hasil menunjukkan bahwa suhu penyimpanan berpengaruh signifikan terhadap nilai trombosit dan PDW. Selain itu, interaksi antara waktu penundaan dan suhu juga menunjukkan perbedaan yang signifikan. Penyimpanan pada suhu ruang menyebabkan penurunan jumlah trombosit dan peningkatan PDW seiring lamanya waktu penundaan, sedangkan penyimpanan pada suhu 4–8 °C menunjukkan perubahan yang minimal. Temuan ini menegaskan bahwa waktu dan suhu penyimpanan berperan penting dalam menjaga keandalan hasil pemeriksaan trombosit dan PDW.
Co-Authors Aprilianti, Ni Kadek Dwi Dewi Puspita Apsari Dewi Puspita Apsari Diah Prihatiningsih I K. Putra Juliantara Iswari, Ni Wayan Gita Jeharu, Sofiana Agustin Lestari, Ni Kadek Yuni Ni Luh Putu Ari Sukmayanti Ni Putu Widayanti Ni Wayan Trisnadewi, Ni Wayan Pramesti, Theresia Anita Putri, Maylina Putu Gede Subhaktiyasa Sashmita, Geby Setiawati, Ni Putu Dinda Sita, A.A. Sagung Ranya Sri Idayani, Sri Sukmayanti, Ni Luh Putu Ari Sukmayanti, Ni Luh Putu Ari Utami, A. A Sagung Ranya Sita Damara Wijayanti, Sariati