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All Journal Journal of Moeslim Research Technik Research of Scientia Naturalis
Schneider, Anna
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Chemistry Control & Systems Engineering Electrical & Electronics Engineering Engineering Physics

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ISOLATION AND CHARACTERIZATION OF NOVEL ANTIBIOTIC-PRODUCING ACTINOBACTERIA FROM THE RHIZOSPHERE OF INDONESIAN MANGROVE FORESTS Triwahyuningsih, Nike; Mei, Chen; Schneider, Anna
Research of Scientia Naturalis Vol. 2 No. 5 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/scientia.v2i5.2567

Abstract

The rise of antimicrobial resistance is a global health crisis, demanding the urgent discovery of novel antibiotics. Indonesian mangrove forests, as a unique and underexplored ecosystem, represent a promising frontier for bioprospecting novel microorganisms. The plant rhizosphere, a zone of intense microbial activity, is particularly rich in actinobacteria, a phylum renowned for its prolific production of bioactive secondary metabolites. This research aimed to isolate and characterize novel antibiotic-producing actinobacteria from the rhizosphere of Indonesian mangrove plants. Rhizosphere soil samples were collected, and actinobacteria were isolated using selective media. All isolates were screened for antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans via the agar well diffusion method. The most potent isolate was subsequently characterized using morphological, biochemical, and 16S rRNA gene sequencing. From 72 distinct isolates, 15 displayed antimicrobial activity. One isolate, designated MGR-17, demonstrated exceptionally potent, broad-spectrum inhibition against all tested pathogens. Based on polyphasic taxonomy, MGR-17 was identified as a potentially novel species of the genus Streptomyces. In conclusion, the rhizosphere of Indonesian mangroves is a fertile source for discovering unique actinobacteria capable of producing novel antibiotics, and the discovery of Streptomyces sp. MGR-17 underscores this potential.
ARCHITECTURAL ENGINEERING IN THE DIGITAL ERA: PARAMETRIC DESIGN AND STRUCTURAL RATIONALIZATION Prabawasari, Veronika Widi; Takahashi, Haruto; Baharuddin, Faizal; Schneider, Anna
Journal of Moeslim Research Technik Vol. 3 No. 2 (2026)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/technik.v3i2.3624

Abstract

Architectural engineering in the digital era is increasingly shaped by parametric design methodologies that enable complex form generation and performance-driven optimization. Rapid advancements in computational tools have transformed design processes, yet a persistent gap remains between architectural exploration and structural rationalization, often resulting in inefficiencies and post-design adjustments. This study aims to develop an integrated computational framework that aligns parametric design with structural performance, ensuring that architectural forms are both innovative and structurally feasible. A computational design-based methodology was employed, combining parametric modeling, finite element analysis, and algorithmic optimization across representative architectural typologies. Iterative workflows were implemented to establish continuous feedback between geometric parameters and structural responses. Results indicate that integrated parametric-structural models achieve higher structural efficiency, reduced material consumption, and improved deformation control compared to conventional and non-integrated approaches. Statistical analysis confirms significant performance improvements, while case-based validation demonstrates strong alignment between simulated and expected structural behavior. Findings further reveal that real-time integration enhances design adaptability and decision-making efficiency. This study concludes that the integration of parametric design and structural rationalization represents a robust and scalable paradigm for contemporary architectural engineering, offering significant implications for sustainability, performance optimization, and interdisciplinary collaboration.
Co-Authors Faizal Baharuddin Mei, Chen Nike Triwahyuningsih Takahashi, Haruto Veronika Widi Prabawasari