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All Journal Vegetalika Agriprima : Journal of Applied Agricultural Sciences
Khiar Ayatina Hasbi
Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Optimasi Optical Density (OD) Dan Dosis Kanamisin Dalam Transformasi Gen Sucrose Phosphate Synthase (SoSPS1) Pada Anggrek Dendrobium sp. Arsy Chairiyah; Bambang Sugiharto; Purnama Okviandari; Parawita Dewanti; Khiar Ayatina Hasbi
Vegetalika Vol 14, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106229

Abstract

Genetic transformation in plants involves the insertion of isolated foreign genes into the plant genome. Sucrose Phosphate Synthase (SPS) is a key enzyme in sucrose biosynthesis, the primary sugar serving as an energy source and carbon transport molecule in most plants. Dendrobium orchids, which utilize Crassulacean Acid Metabolism (CAM), often exhibit a slow growth rate, thereby limiting their commercial potential. The insertion of the SPS gene into the orchid genome is expected to optimize the sucrose metabolic pathway, potentially influencing the rate of photosynthesis, carbon allocation, and growth characteristics of the orchid. Optimizing the optical density (OD) of the Agrobacterium suspension and the dosage of kanamycin is crucial for enhancing the success rate of the gene transformation process. This study aimed to determine the optimal concentrations of OD and kanamycin to achieve efficient SoSPS1 gene transformation in orchids. Three-month-old Protocorm-Like Bodies (PLBs) of Dendrobium Glory White were used as the explant source. The transformed explants were subsequently evaluated for the effectiveness of the kanamycin dosage, the percentage of explant shoots, the number of shoots per explant, the transformation efficiency, and phenotypic changes in the transformed plants. The results showed that a transformation efficiency of up to 40% was achieved using an Agrobacterium suspension at an OD600nm of 0.4. A lethal dose (LD50) of 100 mgL-1 kanamycin resulted in 34% of plantlets surviving post-selection. Transformation with the SoSPS1 gene in Dendrobium sp. induced distinct phenotypic plants including split leaves, swollen stems (pseudobulb), and increased shoot multiplication.
Perbedaan Karakter Agronomi dan Morfologi Tanaman Anggrek Dendrobium Transgenik pada Event T42 dan Wild type Khiar Ayatina Hasbi; Parawita Dewanti; Mohammad Ubaidillah
Agriprima : Journal of Applied Agricultural Sciences Vol 10 No 2 (2026): SEPTEMBER
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v10i2.870

Abstract

Tanaman transgenik hasil transformasi gen Sucrose Phosphate Synthase 1 (SoSPS1) telah diperoleh dan memiliki pertumbuhan yang berbeda dengan wild type namun belum dilakukan karakterisasi agronomi dan morfologi pada tanaman transgenik tersebut. Penelitian ini bertujuan untuk menganalisis pengaruh transformasi gen SoSPS1 terhadap karakter agronomi dan morfologi anggrek Dendrobium transgenik. Penelitian dilakukan melalui eksperimen dengan cara membandingkan planlet transgenik yang membawa gen SoSPS1 dan planlet non-transgenik berdasarkan karakter pertumbuhan vegetatif yang meliputi tinggi tanaman, jumlah daun, luas daun, jumlah akar, panjang akar, serta biomassa tanaman. Data dianalisis secara statistik untuk mengetahui perbedaan respons pertumbuhan antar perlakuan. Hasil penelitian menunjukkan bahwa transformasi gen SoSPS1 meningkatkan pertumbuhan planlet anggrek yang ditunjukkan oleh peningkatan tinggi tanaman yakni 128% , jumlah daun 46%, luas daun 162%, panjang akar 93% serta jumlah akar 60,6%, dan penurunan jumlah tunas 15,92% dibandingkan tanaman wild type. Peningkatan tersebut diduga berkaitan dengan meningkatnya aktivitas enzim SPS yang memacu akumulasi sukrosa sebagai sumber karbon sekaligus molekul sinyal yang mendukung fotosintesis, pembelahan sel, perkembangan sistem perakaran, dan pertumbuhan tajuk. Dengan demikian, transformasi gen SoSPS1 berpotensi meningkatkan vigor planlet anggrek melalui optimalisasi sintesis sukrosa sehingga dapat menjadi salah satu strategi dalam mendukung pengembangan bibit anggrek yang memiliki pertumbuhan vegetatif lebih baik.
Co-Authors Arsy Chairiyah Bambang Sugiharto Mohammad Ubaidillah Parawita Dewanti Purnama Okviandari