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All Journal Biology, Medicine, & Natural Product Chemistry Strada Journal of Pharmacy
Ardhi Broto Sumanto
Universitas Strada Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Investigation of the Anticancer Potential of Vernonia amygdalina Methanol Extract: A New Hope for MDA-MB-231 Breast Cancer Therapy Ivan Junius Mesak; Ardhi Broto Sumanto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.885-893

Abstract

MDA-MB-231 is a breast cancer cell line derived from metastatic adenocarcinoma and classified as a triple-negative breast cancer (TNBC) subtype. This breast cancer subtype is highly aggressive, has a high recurrence rate, and offers limited treatment options due to the absence of estrogen, progesterone, and HER-2 receptor expression. Therefore, the development of advanced therapeutic strategies is urgently needed to inhibit the metastasis of TNBC cancer cells. This study aimed to investigate the potential of the methanol extract of Vernonia amygdalina leaves as a natural chemotherapeutic agent for metastatic breast cancer therapy, with a focus on inducing cell death through apoptosis mechanisms. This study was an experimental research that began with the collection and identification of raw materials, followed by the preparation of simplicia, extraction processes, and phytochemical screening. Subsequently, cytotoxicity testing was conducted using the MTT assay, cell cycle analysis was performed using the PI-staining assay, and apoptosis was assessed using the Annexin V/PI-staining assay, all of which were analyzed through flow cytometry. The methanol extract of V. amygdalina demonstrated a moderate cytotoxic effect with an IC50 value of 109.36 µg/mL. The extract induced S-phase cell cycle arrest in a dose-dependent manner, indicating its ability to reduce cell viability by inhibiting DNA replication. In the apoptosis assay, the highest percentage of cell death (3.12%) was observed at the concentration of 54.5 µg/mL, suggesting that this dose produced the strongest apoptotic response among the treatments tested. The methanol extract of V. amygdalina leaves shows potential as a natural chemotherapeutic agent for metastatic breast cancer. The extract can induce cancer cell death through apoptosis, indicating its promise for further development as a supportive anticancer therapy.
Anti-Acne Activity Evaluation Of Cyperus rotundus L. Rhizome Fraction Gel Against Staphylococcus aureus And Propionibacterium acnes Musdalifah Qadariah; Juvita Herdianty; Luluk Aniqoh Meliana Putri; Ardhi Broto Sumanto
Strada Journal of Pharmacy Vol. 7 No. 2 (2025): October
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjp.v7i2.203

Abstract

Acne is an inflammatory skin condition often caused by bacteria such as Propionibacterium acnes and Staphylococcus aureus. The increasing incidence of antibiotic resistance encourages the search for alternatives from natural ingredients. Nut grass rhizome (Cyperus rotundus L.) is known to have antibacterial compounds. This study aimed to formulate a gel from the fractions of nut grass rhizome, test its activity against acne-causing bacteria, and identify the most potent fraction. This study employed a laboratory experimental design. The nut grass rhizome was extracted with 70% ethanol and then fractionated by liquid-liquid partitioning using n-hexane, ethyl acetate, and water as solvents. Each fraction was formulated into a gel at a 5% concentration, and its antibacterial activity was tested using the well diffusion method. All gel formulas met the physical quality evaluation requirements. The ethyl acetate fraction (F2) exhibited the strongest antibacterial activity, with an average inhibition zone of 19.48 ± 0.99 mm (moderate category) against Staphylococcus aureus and 25.86 ± 0.65 mm (strong category) against Propionibacterium acnes. The nut grass rhizome fraction gel preparation possesses anti-acne activity. The ethyl acetate fraction proved to be the most potent, showing efficacy against Propionibacterium acnes (inhibition zone 25.86 mm) that was statistically equivalent to the positive control (p = 0.767).
Co-Authors Ivan Junius Mesak Juvita Herdianty Musdalifah Qadariah Putri, Luluk Aniqoh Meliana