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All Journal International Journal of Health, Economics, and Social Sciences (IJHESS) Jurnal Kolaboratif Sains
Mahdalena Sy. Pakaya
Dosen program studi S1 Farmasi UNG
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Isolation and Characterization of L-Asparaginase Producing Endophytic Fungi from Plants of the Amaryllidaceae Family Asri Setiani Kadir; A. Mu’thi Andy Suryadi; Mahdalena Sy. Pakaya; Faramita Hiola; Wiwit Zuriati Uno
International Journal of Health, Economics, and Social Sciences (IJHESS) Vol. 8 No. 3: 2026
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/ijhess.v8i3.11124

Abstract

L-asparaginase is an enzyme that catalyzes the conversion of L-asparagine into L-aspartate and inhibits the growth of leukemia cells that depend on asparagine. This study aims to isolate and characterize endophytic fungi from plants of the Amaryllidaceae family, and to evaluate the ability of these isolates to produce the L-asparaginase enzyme. This study employed an experimental method conducted in a pharmaceutical microbiology laboratory. Endophytic fungi were isolated using surface sterilization techniques, followed by cultivation on Potato Dextrose Agar (PDA) media. Enzyme production was performed in liquid fermentation, whereas enzyme purification was conducted using ammonium sulfate fractionation with concentrations of 0–20%, 20–40%, 40–60%, 60–80%, and 80–100%. The activity of L-asparaginase enzyme was tested using the Nesslerization method, with Asparagus used as an inducer. The findings showed that several endophytic fungal isolates produced L-asparaginase, as indicated by a yellowish-brown color in the Nessler test. The purified fractions exhibited varying enzyme activities at different levels of ammonium sulfate saturation. Therefore, endophytic fungi from plants of the Amaryllidaceae family have potential as alternative sources of the L-asparaginase enzyme, which can be further developed in pharmacy and biotechnology.
Perbandingan Produksi Enzim Selulase Dari Aspergillus niger Dengan dan Tanpa Menggunakan Penginduksi Carboxymethyl Cellulose (CMC): Comparison of Cellulase Enzyme Production from Aspergillus niger With and Without Using Carboxymethyl Cellulose (CMC) Inducer Aldevi Trisnawati Adam; Mahdalena Sy. Pakaya; Faramita Hiola; La Ode Aman; Wiwit Zuriati Uno
Jurnal Kolaboratif Sains Vol. 8 No. 9: September 2025
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v8i9.8536

Abstract

Enzim selulase merupakan enzim yang mampu mendegradasi selulosa dengan produk utamanya yakni glukosa, selobiosa dan xilooligosakarida, selulase memiliki sistem enzim yang tersusun dari endo-1,4-?-glukanase, ekso-1,4-?-glukanase dan ?-D-glukosidase. Penelitian ini bertujuan untuk memproduksi enzim selulase dari Aspergillus niger dengan menggunakan penginduksi berupa CMC. Proses produksi dilakukan dengan menggunakan metode fermentasi cair dengan dan tanpa penambahan inducer. Aktivitas enzim selulase diuji secara kualitatif dilihat berdasarkan pembentukan zona hidrolitik. Hasil penelitian menunjukan bahwa enzim selulase yang diproduksi menggunakan inducer CMC membentuk zona hidrolitik lebih besar dibandingkan dengan tidak menggunakan inducer dengan nilai indeks aktivitas enzim secara berturut-turut sebesar 2,5 mm dan 1,93 mm. Dapat disimpulkan bahwa Aspergillus niger dapat menghasilkan enzim selulase lebih banyak dengan penambahan inducer CMC dibandingkan dengan tidak menggunakan inducer
Co-Authors A. Mu’thi Andy Suryadi Aldevi Trisnawati Adam Asri Setiani Kadir Faramita Hiola La Ode Aman Wiwit Zuriati Uno