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All Journal Arfak Chem: Chemistry Education Journal Jurnal Abdi Negeriku
Nur Jati Jagad
Institut Teknologi dan Kesehatan Muhammadiyah Kalimantan Barat
Humanities Chemistry Education Environmental Science Social Sciences Other

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EDUKASI PROBIOTIK SEBAGAI IMMUNOSTIMULANT DAN PENCEGAHAN DYSBIOSIS SERTA OPTIMALISASI POTENSI TUMBUHAN LOKAL SEBAGAI SUMBER PREBIOTIK HABITAT PROBIOTIK Hasria Alang; Anggraini Putri Utami; Nur Jati Jagad
Jurnal Abdi Negeriku Vol 5, No 1 (2026): Jurnal Abdi Negeriku
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jan.v5i1.83959

Abstract

Education about the role of probiotics and the optimization of prebiotics to support probiotics is necessary. Prebiotics are foods or drinks that, when consumed, will improve a person's quality of life. However, due to the perception of partners that probiotics are expensive, and partners' ignorance about the factors supporting probiotic growth, steps are needed to educate partners so they can understand prebiotics and probiotics, which ultimately will improve their health. The purpose of this community service activity is to educate partners about the benefits of probiotics and prebiotics, provide education and examples of probiotic and prebiotic sources that can be obtained from the surrounding environment. The method used is ABCD (Asset-Based Community Development), which utilizes and optimizes the potential of human resources and natural resources. The steps of the PKM activity include preparation, implementation, and evaluation. The results of the intervention showed that this activity increased partners' knowledge from 47% to 95%. This activity also showed high enthusiasm from partners, as seen from active discussions. The results of testimonials showed that partners hope that activities like this can be carried out regularly.
Isolation of endophytic bacteria and evaluation of fermented avicennia marina fruit filtrate as natural antimicrobials against staphylococcus aureus Galih Dwiki Ramanda; Anggraini Putri Utami; Yuyun Nisaul Khairillah; Nur Jati Jagad; M. Fiqri Baihaqi; Annisa Nasyawa Fahira
Arfak Chem: Chemistry Education Journal Vol. 9 No. 1 (2026): Arfak Chem
Publisher : Universitas Papua, Manokwari, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/accej.v9i1.1318

Abstract

Antimicrobial resistance (AMR) has become a major global health challenge, necessitating the exploration of alternative antimicrobial sources from natural products. This study aimed to determine the optimum fermentation time, characterize the colony morphology and Gram-staining characteristics of endophytic bacterial isolates, and evaluate their antimicrobial activity against Staphylococcus aureus. This study employed a quantitative laboratory experimental approach. Endophytic bacteria were isolated from Avicennia marina fruit through serial dilution and characterized based on colony morphology and Gram-staining characteristics. Antimicrobial activity was evaluated using the disk diffusion method. Five endophytic bacterial isolates (AM41–AM45) were successfully obtained, consisting of three Gram-positive and two Gram-negative isolates. Among them, isolate AM41 exhibited the highest inhibitory activity against S. aureus (19.71 mm), followed by AM45 (8.83 mm) and AM42 (8.00 mm), whereas the remaining isolates showed no inhibitory activity. Differences in antimicrobial activity were observed among the fermentation times tested. The largest inhibition zone was recorded after 72 h of fermentation (21.67 mm), followed by 96 h (20.07 mm), while shorter fermentation periods produced lower inhibitory activity. These findings indicate that endophytic bacterial isolates and fermented filtrates of A. marina fruit possess antimicrobial potential against S. aureus. Under the conditions tested, the highest antimicrobial activity of the fermented filtrate was observed after 72 h of fermentation
Co-Authors Anggraini Putri Utami Annisa Nasyawa Fahira Galih Dwiki Ramanda Hasria Alang M. Fiqri Baihaqi Yuyun Nisaul Khairillah