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All Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ekayanti Mulyawati Kaiin
Research Center for Applied Zoology, National Research and Innovation Agency (BRIN)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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CHARACTERISTICS AND KINEMATICS OF RAM SPERMATOZOA MOVEMENT IN FERTILIZATION MEDIUM USING SERICIN Arindya Andrian; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

This study aimed to evaluate the effectiveness of sericin on quality and kinematics of spermatozoa movement in fertilization medium and substituted for BSA in PEIV. Spermatozoa preparation was done with and without swim-up. Each group was incubated in fertilization medium with BSA, sericin 0.05%, 0.1%, 0.5%, and evaluated after addition of fertilization medium,15, 30, 60 minute. The results showed that the progressive motility values, sericin 0,5% group were higher than other groups (P<0.05). Membrane integrity showed differences between treatments. The VCL value showed no difference except for 0 and 30 minute swim-up group, sericin 0.5% was higher (P<0.05). VAP, VSL, LIN, and STR had no difference. The ALH at 30 minute swim-up and 60 minute without swim-up, sericin 0.5% were higher than others (P<0.05). In conclusion, the addition of sericin 0,5% has potential to replace BSA, sericin 0.5% can maintain quality and show early signs of hyperactivation of spermatozoa.
CHARACTERISTICS AND KINEMATICS OF RAM SPERMATOZOA MOVEMENT IN FERTILIZATION MEDIUM USING SERICIN Arindya Andrian; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to evaluate the effectiveness of sericin on quality and kinematics of spermatozoa movement in fertilization medium and substituted for BSA in PEIV. Spermatozoa preparation was done with and without swim-up. Each group was incubated in fertilization medium with BSA, sericin 0.05%, 0.1%, 0.5%, and evaluated after addition of fertilization medium,15, 30, 60 minute. The results showed that the progressive motility values, sericin 0,5% group were higher than other groups (P<0.05). Membrane integrity showed differences between treatments. The VCL value showed no difference except for 0 and 30 minute swim-up group, sericin 0.5% was higher (P<0.05). VAP, VSL, LIN, and STR had no difference. The ALH at 30 minute swim-up and 60 minute without swim-up, sericin 0.5% were higher than others (P<0.05). In conclusion, the addition of sericin 0,5% has potential to replace BSA, sericin 0.5% can maintain quality and show early signs of hyperactivation of spermatozoa.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Asman Ramadhan Damanik; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Asman Ramadhan Damanik; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
Co-Authors Arindya Andrian Asman Ramadhan Damanik M Agus Setiadi Mohamad Agus Setiadi Ni Wayan Kurniani Karja