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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ni Wayan Kurniani Karja
Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells Fahrudin, Mokhamad; Karja, Ni Wayan Kurniani; Suzuki, Tatsuyuki
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.341 KB)

Abstract

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling
Nuclear Maturation of Porcine Oocytes in vitro: Effect of the Cumulus-Oocyte Complexes Quality Karja, Ni Wayan Kurniani
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

The objective of this study was to examine the effect of the cumulus-oocyte complexes (COCs) quality on the ability of porcine oocytes to mature in vitro. Porcine COCs were collected from 2-6 mm follicles of slaughterhouse ovaries. The oocytes used for IVM were classified into three categories based on the compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The oocytes were then matured in vitro for 44 h. At 22 of maturation culture, most of the oocytes in all</div><div>groups were identified still at germinal vesicle (GV) stage and metaphase I (M-I) stage. After 44 h of culture, a greater proportion of Category I and II oocytes completed in vitro maturation through the second meiotic as compared with that of Category III oocytes (P<0.05). The proportion of oocytes remaining at M-I stage and the degenerative oocytes in Category III oocytes were significantly higher than those of oocytes in other groups (P<0.05). These data indicate that porcine oocytes with high quality cytoplasm and a cumulus cell complement have a much greater chance of maturing in vitro than that lower quality oocytes. The morphological grading of immature oocytes is an appropriate selection criterion for their developmental ability.
Developmental Competence of Early Stage Porcine Embryos Cultured in Medium with Different Energy Substrate in vitro Karja, Ni Wayan Kurniani; Kikuchi, Kazuhiro; Fahrudin, Mokhamad
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

To elucidate the effect of energy requirement during the early embryonic development on their developmentalability to develop to blastocyst stage, in vitro fertilized (IVF) porcine one-cell embryos were cultured in modifiedNorth Carolina State University (NCSU)-37 supplemented with different energy substrate. Result indicated that thecleavage rate of embryos in Pyr-Lac and Gluc-Pyr-Lact groups was significantly higher than in those in Gluc groupand Gluc-Rib group (P < 0.05). At Day 6 of culture, the highest proportion of embryos develop to the blastocyst stagewas obtained in the presence of pyruvate-lactate only. In the medium with glucose, the addition of pyruvate-lactateor ribose slightly increased the proportion of embryos develop to the blastocyst stage, however the value were notsignificantly different form those obtained in the presence of glucose only. The mean cell number in blastocystsderived from Pyr-Lac and Gluc-Pyr-Lact groups were significantly higher than those in the Gluc group (P < 0.05).These results indicated that the presence of glucose only, as energy substrate, during the first 2 days of in vitro culture(IVC) caused a decrease in development of in vitro produced (IVP) porcine embryos to the blastocyst stage and meancell number in blastocysts .Keywords: porcine embryos-energy substrate-in vitro culture
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro NI WAYAN KURNIANI KARJA; MOKHAMAD FAHRUDIN; KAZUHIRO KIKUCHI
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.946 KB) | DOI: 10.4308/hjb.16.1.25

Abstract

In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 µM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 µM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 µM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop. Key words: iodoacetate, glucose, early stage embryos, in vitro culture
Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro Feni Dwi Kartika Gulo; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.290

Abstract

Low nuclear maturation and fertilization rate is one obstacle in the in vitro embryo production which decrease embryo yield. This problem is presumable related with high production of reactive oxygen species (ROS) during maturation process. Glutathione (GSH) as an antioxidant is well known to overcome effect of ROS production. GSH synthesis in the cytosol part of the oocyte cytoplasm is influenced by cysteine availability. It is therefore, this research was conducted to evaluate the ability of cysteamine to provide cysteine availability as GSH precursor on the nuclear maturation and fertilization rate of sheep oocytes. Results of this experiment revealed additional cysteamine at 150 µm and 200 µm could significantly improve nuclear maturation rate. On the other side, although additional of cysteamine at 50 µm could not improve nuclear maturation rate, however 50 µm cysteamine in the maturation medium could significantly improve the fertilization rate. Based on those experiment results, it seems that the additional cysteamine might be improve not only GSH availability but also the oocyte quality which characterized the ability of pronuclear formation. This finding strongly suggested that additional cysteamine in the maturation medium could improve nuclear maturation and fertilization rate of sheep oocytes.
Kualitas Oosit Domba dari Ovarium setelah Penyimpanan pada Suhu dan Periode Waktu yang Berbeda Dhia Mardhia Engcong; Ni Wayan Kurniani Karja
Acta VETERINARIA Indonesiana Vol. 1 No. 2 (2013): Juli 2013
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7129.431 KB) | DOI: 10.29244/avi.1.2.45-50

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik oosit domba dari ovarium yang disimpan pada suhu dan periode penyimpanan yang berbeda. Ovarium dari rumah potong hewan dibagi menjadi tiga kelompok. Ovarium disimpan dalam larutan NaCl 0,9 % pada suhu 4, 27-28, dan 37-38 °C selama 2, 5-7, dan 8-10 jam untuk masih-masing suhu penyimpanan. Pada setiap akhir periode penyimpanan, oosit dikoleksi dari ovarium dengan menggunakan metode penyayatan. Oosit kemudian diseleksi dan dibagi menjadi 4 (empat) kelompok yaitu kelompok A, B, C, dan D berdasarkan lapisan sel kumulus dan gambaran sitoplasmanya. Oosit dengan kualitas A yang dikoleksi dari ovarium 2 jam dan 5-7 jam (P>0,05) tidak berbeda, tetapi kemudian menurun setelah penyimpanan 8-10 jam (P
Kualitas Spermatozoa Domba yang Disentrifugasi sebelum Dibekukan dengan Pengencer Laktosa Pasca-Thawing Latifah Humairoh; Ni Wayan Kurniani Karja
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.994 KB) | DOI: 10.29244/avi.2.1.31-35

Abstract

Telah diketahui bahwa plasma semen dapat menurunkan motilitas spermatozoa selama prosespembekuan. Sentrifugasi adalah metode yang digunakan untuk memisahkan plasma semen padaspermatozoa. Oleh karena itu, dilakukan penelitian pada spermatozoa domba yang dibekukan dandiberi perlakuan sentrifugasi untuk memisahkan plasma semen dan spermatozoa domba. Terdapattiga perlakuan pada sampel spermatozoa domba yaitu kontrol (tanpa sentrifugas) dan sentrifugasidilakukan selama 5 menit dan 10 menit dengan kecepatan 3000 rpm. Ketiga sampel diekuilibrasi selama2 jam pada suhu 4 ºC, kemudian dibekukan dengan nitrogen cair dan di thawing pada suhu 37 ºC selama30 detik. Hasil yang didapat dengan menggunakan program analisis data SPSS dengan Anova dan ujilanjut Duncan menunjukkan bahwa tidak ada perbedaan nyata pada motilitas, viabilitas, dan integritasmembran plasma utuh (MPU) antara spermatozoa dari kelompok kontrol dan perlakuan setelahthawing (P>0,05). Dari data tersebut dapat disimpulkan bahwa keberadaan plasma semen selamaproses pembekuan tidak mempunyai pengaruh negatif terhadap kualitas spermatozoa domba pascapembekuan.
Daya Fertilisasi Spermatozoa Kauda Epididimis Domba dengan atau tanpa Swim Up sebelum Fertilisasi Nur’aisyah Amrah Safitri; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Mokhamad Fahrudin
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.208 KB) | DOI: 10.29244/avi.5.1.1-7

Abstract

Penelitian ini dilakukan untuk mengevaluasi penggunaan metode swim up untuk persiapan spermatozoa sebelum fertilisasi terhadap tingkat fertilisasi in vitro spermatozoa kauda epididimis pasca penyimpanan selama 48 jam. Kauda epididimis domba disimpan pada suhu 4 oC selama 0 hari (H-0), 1 hari (H-1) dan 2 hari (H-2), kemudian spermatozoa dikoleksi dan dibekukan. Spermatozoa ejakulat beku digunakan sebagai kontrol. Oosit yang telah matang difertilisasi secara in vitro dengan spermatozoa asal kauda epididimis pasca penyimpanan dan ejakulat menggunakan metode persiapan spermatozoa dengan dan tanpa swim up. Hasil penelitian menunjukkan bahwa spermatozoa asal kauda epididimis yang dikoleksi segera setelah kematian hewan (H-0) memiliki kemampuan yang sama dengan spermatozoa ejakulat (P>0,05). Tingkat fertilisasi spermatozoa kauda epididimis pasca penyimpanan selama 2 hari mengalami penurunan seiring bertambahnya waktu simpan. Penggunaan metode swim up dan tanpa swim up menunjukkan kemampuan fertilisasi yang sama pada spermatozoa ejakulat dan spermatozoa kauda epididimis yang disimpan. Dapat disimpulkan bahwa metode swim up tidak berpengaruh terhadap tingkat fertilisasi in vitro spermatozoa asal kauda epididimis yang disimpan pada suhu 4 °C selama 2 hari. Kemampuan fertilisasi spermatozoa asal kauda epididimis domba yang disimpan pada suhu 4 °C mengalami penurunan sampai hari kedua, namun spermatozoa tersebut masih mampu membuahi oosit secara in vitro.
Status Akrosom dan Kualitas Post-Thawed Spermatozoa pada Beberapa Rumpun Sapi dari Dua Balai Inseminasi Buatan Yulida Nofa; Ni Wayan Kurniani Karja; Raden Iis Arifiantini
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.416 KB) | DOI: 10.29244/avi.5.2.81-88

Abstract

Tujuan dari penelitian ini adalah untuk mengevaluasi kualitas dan status akrosom spermatozoa semen beku pasca-thawing beberapa rumpun sapi dari dua Balai Inseminasi Buatan (BIB). Semen beku dari sapi limousin, ongole, simental dan brahman yang di-thawing di dalam air hangat (37 oC) selama 30 detik dan kemudian dievaluasi motilitas spermatozoa, viabilitas, dan membran plasma utuh  (MPU). Evaluasi Status akrosom spermatozoa menggunakan pewarnaan Trypan Blue - Giemsa (TBG) dan Coomassie Blue (CB). Hasil penelitian menunjukkan bahwa motilitas (51 ± 1,87%) dan MPU spermatozoa (72,53 ± 1,68%) dari sapi ongole lebih tinggi daripada rumpun sapi  yang lainnya pada BIB A (P<0,05). Namun, dari BIB B, motilitas dan MPU dari limousin lebih tinggi dari rumpun lainnya (P <0,05) dengan persentase masing-masing 51 ± 1,58% dan 72,60 ± 0,62%. Spermatozoa dengan TAU (95,40 ± 0,79%) dari rumpun sapi ongole menggunakan CBB G250 pada BIB B lebih tinggi dibandingkan 3 rumpun sapi lainnya (P<0,05). Akrosom spermatozoa dapat dievaluasi menggunakan TBG dan CBB G250. Namun, TBG lebih kompleks dan untuk mendapatkan hasil yang memuaskan membutuhkan jangka panjang.
Efektivitas Insulin-Like Growth Factor-I (IGF-I) dalam Media Maturasi In Vitro Pada Pematangan Inti dan Fertilisasi Oosit Sapi Bali . Hasbi; Sri Gustina; Ni Wayan Kurniani Karja; Iman Supriatna; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.643 KB) | DOI: 10.29244/avi.6.1.24-29

Abstract

Penelitian ini bertujuan mengetahui efektivitas insulin-like growth factor–I (IGF-I) pada tingkat pematangan inti dan fertilisasi oosit sapi bali. Penelitian ini dibagi dalam dua tahap. Tahap I, oosit dimatangkan secara in vitro dalam media 199 dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I. Tahap II, oosit dimatangkan dalam media seperti pada penelitian tahap I, kemudian difertilisasi secara in vitro untuk mengamati pembentukan pronukleus. Hasil penelitian tahap I menunjukkan bahwa tidak terdapat perbedaan jumLah oosit yang mencapai tahap metaphase II (MII) dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I dalam media maturasi. Berturut-turut adalah 80,6±7,6%; 81,5±8,6%; 87,5±6,9%; dan 84,1±12,4%. Penelitian tahap II menunjukkan bahwa tingkat fertilisasi pada penambahan 100 ng/mL IGF-I dalam media maturasi nyata lebih tinggi (P<0,05) dibandingkan dengan kontrol dan penambahan 50 ng/mL IGF-I, yaitu berturut-turut 78,3±6,6%, 67,1±8,9%, dan 64,6±6,0% untuk dosis 100, 0, dan 50 ng/mL. Akan tetapi, peningkatan dosis pemberian IGF-I menjadi 150 ng/mL tidak meningkatkan tingkat fertilisasi yaitu 73,5±9,3%. Dari hasil penelitian disimpulkan bahwapenambahan IGF-I dalam media maturasi tidak mampu meningkatkan jumLah oosit yang mencapai tahap MII, namun penambahan 100 ng/mL IGF-I dapat meningkatkan jumLah oosit yang terfertilisasi.
Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin